DYKDDDDK tag Recombinant monoclonal antibody (Binds to FLAG? tag epitope) proteintech 80801-2-RR
$189.00
In stock
SKU
80801-2-RR
3Flag, Ag2329, Flag, DDDDK, DDDDK tag
| Host / Isotype: Rabbit / IgG | Class: Recombinant |
| Reactivity: Recombinant Protein And More (1) | Immunogen: CatNo: Ag2329 Product name: Recombinant DYKDDDDK tag protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: flag of Sequence: DYKDDDDK Predict reactive species |
| Applications: WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA | |
| Formulation: PBS, Azide, Glycerol | GenBank Accession Number: AB_3670488 |
| Conjugate: Unconjugated | Gene Symbol: Unconjugated |
| Tested Applications: Positive WB detected in | Gene ID (NCBI): Liquid |
| Application: Western Blot (WB) | RRID: Protein A purfication |
| Dilution: WB : 1:5000-1:50000 | Conjugate: FLAGTAG |
| Tested Reactivity: Recombinant Protein | Form: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Host / Isotype: Rabbit / IgG | Background Information: Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal DDDDK vectors provide an Ek cleavage site for removal of the fusion tag. The DDDDK peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the DDDDK peptide is more likely to be accessible to antibodies. A DDDDK-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on. This antibody is a rabbit recombinant antibody raised against 3xFlag (3xDYKDDDDKT) sequence and recognizes the (1x) and (3x)DYKDDDDK peptide and detects DDDDK-tagged proteins. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology. |
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