CD146/MCAM Monoclonal antibody proteintech 66153-1-Ig
$449.00
In stock
SKU
66153-1-Ig
CD146, 4D8A9, MUC18, Melanoma-associated antigen MUC18, Melanoma-associated antigen A32
| Host / Isotype: Mouse / IgG1 | Class: Monoclonal |
| Reactivity: human | Immunogen: CatNo: Ag11855 Product name: Recombinant human CD146, MCAM protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 26-350 aa of BC056418 Sequence: GEAEQPAPELVEVEVGSTALLKCGLSQSQGNLSHVDWFSVHKEKRTLIFRVRQGQGQSEPGEYEQRLSLQDRGATLALTQVTPQDERIFLCQGKRPRSQEYRIQLRVYKAPEEPNIQVNPLGIPVNSKEPEEVATCVGRNGYPIPQVIWYKNGRPLKEEKNRVHIQSSQTVESSGLYTLQSILKAQLVKEDKDAQFYCELNYRLPSGNHMKESREVTVPVFYPTEKVWLEVEPVGMLKEGDRVEIRCLADGNPPPHFSISKQNPSTREAEEETTNDNGVLVLEPARKEHSGRYECQGLDLDTMISLLSEPQELLVNYVSDVRVSP Predict reactive species |
| Applications: WB, IHC, IF/ICC, IF-P, ELISA | Observed Molecular Weight: 646 aa, 72 kDa |
| Formulation: PBS, Azide, Glycerol | GenBank Accession Number: BC056418 |
| Conjugate: Unconjugated | Gene Symbol: CD146 |
| Tested Applications: Positive WB detected in | Gene ID (NCBI): 4162 |
| Application: Western Blot (WB) | RRID: AB_2881549 |
| Dilution: WB : 1:2000-1:20000 | Conjugate: Unconjugated |
| Tested Reactivity: Human | Form: Liquid |
| Host / Isotype: Mouse / IgG1 | Background Information: CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988). This antibody detects a band at approximately 120 kDa that corresponds to the molecular weight of glycosylated CD146. Treatment of lysates of HepG2 cells and L02 cells with PNGase F, which removes oligosaccharides from N-linked glycoproteins, led to a down-shift of the detected band. |
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