Phospho-AKT (Ser473) Polyclonal antibody proteintech 28731-1-AP
$449.00
In stock
SKU
28731-1-AP
AKT (Ser473), p AKT, p AKT (Ser473), p-AKT, p-AKT (Ser473)
| Host / Isotype: Rabbit / IgG | Class: Polyclonal |
| Reactivity: Human, Mouse, Rat And More (3) | Immunogen: Peptide Predict reactive species |
| Applications: WB, IHC, IF/ICC, ELISA | Observed Molecular Weight: 58 kDa |
| Formulation: PBS, Azide, Glycerol | GenBank Accession Number: AKT1 |
| Conjugate: Unconjugated | Gene Symbol: 207 |
| Tested Applications: Positive WB detected in | Gene ID (NCBI): AB_2881201 |
| Application: Western Blot (WB) | RRID: Unconjugated |
| Dilution: WB : 1:1000-1:5000 | Conjugate: Liquid |
| Tested Reactivity: Human, Mouse, Rat | Form: Antigen affinity purification |
| Host / Isotype: Rabbit / IgG | Background Information: Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Activated TNK2 phosphorylates it on Tyr-176 resulting in its binding to the anionic plasma membrane phospholipid PA (PubMed:20333297). This phosphorylated form localizes to the cell membrane, where it is targeted by PDPK1 and PDPK2 for further phosphorylations on Thr-308 and Ser-473 leading to its activation (PubMed:9512493). Ser-473 phosphorylation by mTORC2 favors Thr-308 phosphorylation by PDPK1 (PubMed:21464307, PubMed:8978681). AKT1 Thr-308 and Ser-473 is Phosphorylated by IKBKE and TBK1 (PubMed:15718470, PubMed:18456494, PubMed:20481595, PubMed:8978681). Ser-473 phosphorylation is enhanced by interaction with AGAP2 isoform 2 (PIKE-A) (PubMed:14761976). Ser-473 phosphorylation is enhanced in focal cortical dysplasias with Taylor-type balloon cells (PubMed:17013611). Ser-473 phosphorylation is enhanced by signaling through activated FLT3 (By similarity). Ser-473 is dephosphorylated by PHLPP (PubMed:28147277) and Ser-473 by PP2A phosphatase (PubMed:21329884). |
Reviews
Write Your Own Review