LC3B-Specific Polyclonal antibody proteintech 18725-1-AP
$449.00
In stock
SKU
18725-1-AP
LC3, MAP1LC3B, ATG8F, Autophagy-related ubiquitin-like modifier LC3 B, LC3B
| Host / Isotype: Rabbit / IgG | Class: Polyclonal |
| Reactivity: Human, Mouse, Rat And More (3) | Immunogen: Peptide Predict reactive species |
| Applications: WB, IHC, IF/ICC, FC (Intra), IP, ELISA | Observed Molecular Weight: 15 kDa |
| Formulation: PBS, Azide, Glycerol | GenBank Accession Number: NM_022818 |
| Conjugate: Unconjugated | Gene Symbol: LC3B |
| Tested Applications: Positive WB detected in | Gene ID (NCBI): 81631 |
| Application: Western Blot (WB) | RRID: ENSG00000140941 |
| Dilution: WB : 1:500-1:3000 | Conjugate: AB_2137745 |
| Tested Reactivity: Human, Mouse, Rat | Form: Unconjugated |
| Host / Isotype: Rabbit / IgG | Background Information: LC3B, also named as MAP1LC3B, MAP1A/1BLC3, belongs to the MAP1 LC3 family. It is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. In cell biology, autophagy, or autophagocytosis, is a catabolic process involving the degradation of a cell's own components through the lysosomalmachinery. It is a major mechanism by which a starving cell reallocates nutrients from unnecessary processes to more-essential processes. Two forms of LC3, called LC3-I (17-19kd) and -II(14-16kd), were produced post-translationally in various cells. LC3-I is cytosolic, whereas LC3-II is membrane bound. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. The amount of LC3-II is correlated with the extent of autophagosome formation. LC3-II is the first mammalian protein identified that specifically associates with autophagosome membranes. MAP1LC3 has 3 isoforms MAP1LC3A, MAP1LC3B and MAP1LC3C. MAP1LC3A and MAP1LC3C are produced by the proteolytic cleavage after the conserved C-terminal Gly residue, like their rat counterpart, MAP1LC3B does not undergo C-terminal cleavage and exists in a single modified form. This antibody is specific to LC3B. |
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