pECMV-3×FLAG-UBE2O
$99.00
In stock
SKU
PL85296
NM_022066.3;E2-230K
100μg liquid plasmid(Instantly):$120
| name | pECMV-3×FLAG-UBE2O | another name | apECMV-3×FLAG-UBE2O |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F： | |
| 3′ sequencing primer | BGH： | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCCACCAT GGACTACAAA GACCATGACG GTGATTATAA AGATCATGAC ATCGACTACA AGGATGACGA TGACAAGCTT GGTACCGAGC TCGGATCCAC TAGTCCAGTG TGGTGGAATT CTATGGCGGA TCCCGCAGCC CCCACGCCCG CAGCTCCCGC TCCAGCCCAG GCCCCGGCTC CAGCCCCGGA GGCAGTCCCG GCCCCAGCCG CAGCCCCCGT CCCGGCGCCG GCGCCCGCCT CGGACTCGGC CTCCGGGCCG TCCTCGGACT CCGGCCCAGA AGCCGGCTCG CAGCGCCTGC TGTTTTCTCA CGACCTGGTG TCGGGCCGTT ACCGTGGCTC CGTGCACTTC GGGCTGGTGC GCCTCATCCA CGGCGAGGAC TCGGACTCGG AGGGCGAGGA GGAGGGCCGC GGGAGCTCGG GGTGCTCCGA GGCCGGGGGC GCGGGCCACG AGGAGGGCCG GGCCAGCCCC CTGCGCCGCG GCTACGTGCG CGTCCAGTGG TACCCGGAGG GCGTCAAGCA GCATGTGAAG GAGACCAAGC TGAAACTAGA GGACCGTTCT GTGGTGCCCC GAGATGTGGT CCGGCACATG CGATCCACCG ACAGTCAGTG TGGCACGGTG ATCGACGTCA ACATCGACTG TGCCGTCAAG CTCATCGGCA CCAACTGCAT CATCTATCCC GTCAACAGCA AGGACCTGCA GCACATCTGG CCCTTCATGT ATGGGGACTA CATTGCCTAT GACTGCTGGC TGGGGAAGGT CTACGACTTG AAGAACCAGA TCATCCTGAA GCTATCCAAC GGCGCCAGGT GCTCCATGAA CACGGAAGAT GGCGCCAAGC TCTACGACGT CTGCCCGCAC GTCAGCGACT CGGGTCTCTT CTTCGATGAT TCCTATGGCT TCTACCCAGG CCAGGTGCTC ATTGGCCCTG CCAAGATCTT CTCCAGCGTC CAGTGGCTGT CAGGTGTCAA GCCCGTGCTC AGCACCAAGA GCAAGTTCCG AGTGGTGGTG GAAGAGGTGC AGGTTGTAGA GTTGAAAGTT ACATGGATTA CCAAGAGTTT CTGTCCAGGG GGCACGGACA GCGTCAGCCC CCCACCCTCT GTCATCACCC AGGAAAACCT AGGCAGGGTG AAGCGTCTCG GATGCTTTGA CCATGCTCAG CGGCAGCTTG GGGAGCGCTG TCTGTATGTC TTCCCAGCCA AGGTAGAGCC AGCCAAGATT GCCTGGGAAT GTCCAGAAAA AAACTGCGCC CAGGGGGAGG GCTCTATGGC CAAGAAGGTG AAGCGCCTGT TGAAGAAGCA GGTTGTGCGG ATCATGTCAT GCTCCCCAGA CACCCAGTGT TCCCGGGACC ATTCCATGGA AGACCCAGAC AAGAAGGGGG AATCCAAAAC CAAGAGCGAA GCGGAGTCTG CCAGCCCTGA GGAGACGCCC GATGGCTCTG CCAGTCCAGT GGAGATGCAG GACGAGGGTG CAGAGGAGCC CCACGAGGCA GGAGAGCAGC TGCCCCCATT CCTGCTAAAA GAAGGCAGAG ATGACAGGCT GCACTCGGCA GAGCAGGACG CAGATGATGA GGCTGCTGAT GACACGGACG ACACCAGTTC GGTGACCTCC TCTGCCAGCT CCACCACTTC CTCCCAGAGC GGCAGCGGCA CGAGTCGCAA AAAGAGCATC CCCTTGTCCA TCAAGAACTT AAAGCGCAAA CACAAGAGGA AGAAGAATAA AATCACTCGA GACTTCAAGC CAGGGGACAG GGTGGCAGTG GAGGTGGTGA CCACGATGAC CTCAGCCGAC GTGATGTGGC AGGATGGCTC CGTGGAATGC AACATCCGCT CCAACGACCT CTTCCCTGTG CACCACCTGG ACAACAACGA GTTCTGCCCT GGAGACTTCG TGGTAGATAA GCGAGTCCAG AGCTGTCCAG ACCCTGCTGT CTACGGTGTG GTACAGTCTG GGGACCACAT CGGCCGTACC TGCATGGTGA AGTGGTTCAA GCTGAGGCCG AGTGGGGACG ACGTGGAGCT GATTGGAGAA GAGGAAGATG TGAGTGTTTA CGACATTGCT GACCACCCTG ACTTTAGGTT CCGTACAACT GACATCGTCA TCCGCATCGG CAATACTGAG GATGGGGCTC CTCACAAGGA GGATGAGCCA TCGGTGGGCC AGGTGGCCCG TGTGGACGTC AGCAGCAAGG TGGAGGTGGT GTGGGCTGAC AACTCAAAGA CCATCATCCT GCCCCAGCAC TTGTACAACA TAGAGTCTGA GATTGAGGAG TCAGACTACG ATTCGGTAGA AGGCAGCACC AGCGGGGCAT CCTCGGATGA ATGGGAAGAT GATAGTGACA GCTGGGAGAC GGACAATGGG CTGGTGGAGG ACGAGCACCC CAAGATAGAG GAGCCCCCCA TCCCACCCCT GGAGCAGCCG GTGGCCCCTG AGGACAAGGG AGTGGTGATC AGTGAAGAGG CAGCCACAGC TGCCGTCCAG GGGGCTGTGG CCATGGCTGC CCCCATGGCC GGGCTGATGG AGAAGGCTGG CAAGGACGGG CCACCCAAGA GCTTCCGGGA GTTGAAAGAG GCCATCAAGA TCCTGGAGAG CCTCAAGAAC ATGACTGTGG AGCAGCTGCT GACGGGCTCG CCCACCTCTC CGACTGTGGA GCCTGAGAAG CCAACTCGGG AGAAGAAGTT TCTGGATGAC ATCAAGAAGC TACAGGAAAA CCTCAAGAAG ACCCTGGACA ATGTGGCCAT TGTAGAGGAG GAGAAGATGG AAGCAGTGCC CGACGTAGAG CGCAAGGAGG ACAAGCCCGA GGGGCAGTCA CCTGTGAAGG CTGAGTGGCC CAGCGAAACC CCGGTGCTGT GCCAGCAGTG TGGCGGCAAG CCTGGCGTCA CCTTCACCAG CGCCAAGGGC GAGGTCTTCT CCGTACTGGA GTTTGCACCC TCAAATCATT CTTTTAAGAA AATTGAGTTC CAGCCTCCAG AAGCCAAGAA GTTCTTCAGC ACAGTGCGGA AGGAGATGGC GCTGCTGGCT ACCTCACTGC CTGAGGGCAT CATGGTCAAG ACTTTTGAAG ATAGAATGGA CCTCTTCTCA GCTCTCATCA AGGGCCCCAC TCGAACCCCC TACGAGGATG GCCTCTACTT GTTTGACATC CAGCTCCCCA ACATCTACCC AGCCGTGCCC CCCCACTTCT GCTACCTCTC CCAATGCAGT GGCCGCCTGA ACCCCAACCT GTATGACAAT GGGAAGGTGT GTGTCAGCCT CCTGGGCACC TGGATTGGAA AGGGGACAGA GAGGTGGACA AGCAAGTCCA GCCTTCTCCA GGTGCTCATC TCCATCCAAG GTCTGATCCT GGTAAATGAA CCATACTACA ACGAAGCCGG CTTCGACAGT GACCGAGGCC TGCAGGAAGG CTATGAAAAC AGTCGCTGTT ACAATGAGAT GGCGCTGATC CGCGTGGTGC AGTCCATGAC CCAGCTGGTG CGGCGGCCCC CCGAGGTCTT TGAGCAGGAG ATCAGGCAAC ACTTTAGCAC TGGTGGCTGG CGGCTGGTGA ACCGTATCGA GTCCTGGCTG GAAACCCATG CCCTGCTGGA GAAGGCCCAG GCACTGCCCA ACGGGGTGCC CAAGGCCAGC AGCTCGCCAG AGCCCCCAGC TGTAGCCGAG CTGTCAGACT CCGGCCAACA AGAACCTGAG GATGGAGGGC CAGCCCCAGG AGAGGCCTCC CAGGGCTCAG ACTCAGAGGG CGGTGCCCAG GGCCTGGCCT CAGCTAGCAG GGACCACACA GACCAGACTT CGGAGACCGC ACCAGACGCA TCGGTGCCAC CCAGTGTGAA ACCAAAGAAG CGGAGAAAGA GCTACCGGAG CTTCTTACCT GAGAAGAGTG GCTACCCTGA CATCGGCTTC CCCCTCTTCC CACTTTCCAA GGGTTTCATC AAGAGCATCC GGGGTGTCCT GACGCAGTTC CGGGCTGCCC TGCTAGAGGC AGGCATGCCG GAGTGCACAG AGGACAAGTA GGATATCCAG CACAGTGGCG GCCGCTCGAG TCTAGAGGGC CCGTTTAAAC CCGCTGATCA GCCTCGACTG TGCCTTCTAG TTGCCAGCCA TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC TTGACCCTGG AAGGTGCCAC TCCCACTGTC CTTTCCTAAT AAAATGAGGA AATTGCATCG CATTGTCTGA GTAGGTGTCA TTCTATTCTG GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG GAGGATTGGG AAGACAATAG CAGGCATGCT GGGGATGCGG TGGGCTCTAT GGCTTCTGAG GCGGAAAGAA CCAGCTGGGG CTCTAGGGGG TATCCCCACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTAATT CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCTG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGTTCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGTATACC GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTC
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