pECMV-Bmpr2-m-FLAG
$84.00
In stock
SKU
PL85237
100μg liquid plasmid(Instantly):$105
| name | pECMV-Bmpr2-m-FLAG | another name | apECMV-Bmpr2-m-FLAG |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Kan |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | pFLAG-CMV-R：GCACTGGAGTGGCAACTT | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GGAATGCACT TCCAGGGCCA GGAGAGGCAC TGGGGAGGGG TCACAGGGAT GCCACCCGGG ATCTGTTCAG GAAACAGCTA TGACCGCGGC CGGCCGTTTA AACCTTATCG TCGTCATCCT TGTAATCCAG GATATCATTT GCTGCCAGAT CCTCTTCTGA GATGAGTTTC TGCTCGAGCG GCCGCGTACG CGTCAGACAA TTCATTCCTA TATCTTTAGA CACTGTGGTC GTGGCAGTGC CTCCCTCTGC AAGGTACACA GCAGTGCTAG ATTTAGAATG GACTGCCCTG TCACTGCCAT TGTTGTTGAC CTCACAGTCC AGCGGCTCGG TGGAGATGAC CCAGGTGGAC GGGCGCCACC GCTTAAGAGA GTATGGAGTT TTCACACGTC TCTTGATCTT TTCACCTGAT CCTGATTTGC CATCTTGTGT TGACTCACCT ATCTGTATAC TGCTGCCATC CAGGATATTT GTGGCTGAAA GATCCAGAGA ATTGGGCCTC TGTGCTCTTC TGGGCTTTGA TGGCCCAGGA TCTGCAGCTG TGCTTGTAAC ACCTTGTGTA AGGATATCTT GTTCTGAACA TGGATTGCTG TTGTTGTTAT TGGAATTTGT TCGGCCACCT TCTAATGGCC GTTCCCTCCT ATCTACCAAA CGATCCAGAA CCCCTTCATC ATGGCCAGCC TGTTGCTCTC GTCTCAGTAA AGGTTCATGC TCATCAGGAC TGGAATTGAT ATTCAGCCTG GTATCCTCAC CAATAAATTG ATTCTGCAGC ATTTCTTGGG ACCTATGTGC CACTATGTTG GCTGCCTGGC CAGCTGGCAC TGCGCCATTG GCATACTGGG TTGTGGCAGC ATGGGAATTA ACATTGTGGC TTCTACCTGC CACACCATTC ATAGTTACTG TCACCACATG AGGCTCTGCT GCATTGATTG TATTCATCTT GGCAACTCCA GTTTCTACTT GTTTCAAGTT TGATTTGTGC TTGTTGCCAA ATTTTAGCCG GGGTTCTTTT GTTGAATTTT TGGTGTTCAA AGGCAAACTA GTAGGTCTCT TAGGAAGGTT CTGTTGCTTA GGGAGAGGAT AGATCTGAGC AGGTGGAACA TCAGGAATTA AACATGCTTG CCCATTTGCA GCCTGTGTGA AGTCCTGTTG TCCAGTCACT TCCACTGCGA GCTTTATGAG TGGATAAAGC AAGCTAGAAC TGGTACTGCT CAATGGGTCT GGCCCACTGA ACTGCTTCAG AGAATGCTCC ATGAGATTCT CATCAGAGCT TTCCTTGAGG TTCTTATCAA CTTCTTTTGG ATCTAGCTTA TTAGTCTCCA AGTCTTCTTC TGTCAGCTGT AAGCAGACAG GGGTTGGCCC GATTGACTGT GCAACATTTG TGGCGTGCAA ATGTGTCTCA TCTGGGTATG GCATCTCAGA TATAGTGGTC ATGCCAGTAC TTGGAGTGAG GCCGGTGGTG TTTGTGGTGG TTGTGTTTGT GGACAGGCTT GTGACGCTTG TTTCTGGGCT AGGGATTCGA GCTTGTGCTT GCTGTCGTTC ATAATTAATT GAATTTCGAT TCTTTTCTCC TATTGTCAAT GGTGTGCTGG ACATCGAATG CTCAGAGGAA ATATTCTTCA CAATGCTGTC AGTATGATGG ATAGAGTCTT CAATATATGA GGAAGAGGAA TAATCTGGGT AAGGCCCGAT TTTTGGCACA CGCCTATTAT GTGACAGGTT GCGTTCATTC TGCATAGCAG TTGACATTGG GTTGACCGTT GGGCTCACAG ACTTGTTTCT CTCCCATATC ATCATGAGTT CAGCCATCCT CTCCTCAGCA CACTGTGCAG TGAGCCGAGC CTCTGCATCC TGGTCCCAGC AGTCTTCAAT TGTTTCCTTG AGTGACCTCA CTGCCAGGCT ATTTTCTTTC CAGGCTTCTG GGAACTTGGG TCTCTGCTTC TCTCTGGACA CAAGAACCTG CATATCCTCA AATGTGGGAT GGTTTCCAAC TTCTGTCTGA AAAGCCATCT GGTAATCTGG TACAGATTCA CCTGGGAAGA GGTCTGTACA CCTCATAAAC ACCTCCCAGT AGATGAGTCC AAGCGCATAC ATGTCCACTT GCTTCAGAGC TGACTCACAG TCCCTCAGGT TCACAGCTCC TTCTAGCACT TCTGGTGCCA TATAGCGAAT TGTGCCAACC TCACTTATAG CCGCATTATC TTCTTCCCCT GGGCGCACCA GCCGATTTCC AGTTAGCCTC ATGGATAAAC CAAAGTCACT GATAACACAC GCGCCGTCAT TCTTTACCAG GACATTCCTG CTGTTTAAAT CTCGGTGGGA GATTGCGGGT TTATAATGAT CTCCTCGTGG TAATTCTGTG TGAAGATAAG CCAGTCCTCT AGTCACAGAA TGAGCCAGAC GGCAAGAGCT TACCCAATCA CTTGTGTGGA GACTCAGATA TTTGCACAGA GATCCATTGG GATAATACTC CATCACAAGC AAATACTCCA TGCGGCCGTC TGCAGTGAGC CTCTCGTCTC CAACTATGAA GCGAGCAATG TTGTCATGTT CCATCAAAGG CACTCTGTAA ATGTTTTTTT CATTTATAAA ATTCTGACGG TTTGCAAAAG AAAATACTTT TACAGCAACT GGACGCTCAT CCAAGGAACC TTTATATACT GCTCCGTATC GACCCCGTCC AATCAGCTCC AGCAGCTTCA GGTTATCCAG GTCAAGGGAG GGCTCTGCTG CTGCCGCCTC CATCATGTTC ATGCTGTGAA GACCCTGTTT CCGGTCTCCT GTCAACATTC TGTATCCAAA ACATAAGGCG ACTATCAAAA CAGCTAACAC AGAAACTGAT GCCAAAGCAA TGATTATCGT TTCATCTCGA TTAAATGAAT GAGGTGGACT GAGTGGTGTT GTGTCAGGGG GTGGAAAGTT CTCAGTAAAG TTGACATTAC ATAAATCTGT ACTACAGCAG CAAAAGCGGT ACGTTCCATT CTGAATTGAG GGTGGGGTGG TAGTTACTAC ACACTCTTCA TAGTGGCACT CTTGGGGATC ACCGATGTGA GACCAACATC CTTGTTTCAC AAGATTGATG TCCCCTTTTG ATTTCTCCCA CAGACCATAA CACGTGCTCC CTTTGGAACA TAATATTGTC CCATTTTCAT GAGAGATTCG ACTCTCACCT ATCCCAAGAT CTTGTTGATA TGGATCTTTA AATGCACACA GCCGTTCTTG ATTCTGAGAA GCAGCCGTAG TGCTGACCAG CAGGACGGCC CATAGCAGCC AGGGCACCCG AAAGGGCCGA TGCAGCGAGG AAGTCATGGC GATCGCGGCG GCAGATCTCC TCGGTACCGG ATCCAGTCGA CGAATTCCCG GCCGCCCTAT AGTGAGTCGT ATTACAAAAT TCTGACGGTC ACTAAACGAG CTCTGCTATT ACTCCCATG
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