pECMV-C3-m-FLAG
$128.00
In stock
SKU
PL85223
100μg liquid plasmid(Instantly):$149
| name | pECMV-C3-m-FLAG | another name | apECMV-C3-m-FLAG |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Kan |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | pFLAG-CMV-R：GCACTGGAGTGGCAACTT | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGGACCAGCT TCAGGGTCCC AGCTACTAGT GCTACTGCTG CTGTTGGCCA GCTCCCCATT AGCTCTGGGG ATCCCCATGT ATTCCATCAT TACTCCCAAT GTCCTACGGC TGGAGAGCGA AGAGACCATC GTACTGGAGG CCCACGATGC TCAGGGTGAC ATCCCAGTCA CAGTCACTGT GCAAGACTTC CTAAAGAGGC AAGTGCTGAC CAGTGAGAAG ACAGTGTTGA CAGGAGCCAG TGGACATCTG AGAAGCGTCT CCATCAAGAT TCCAGCCAGT AAGGAATTCA ACTCAGATAA GGAGGGGCAC AAGTACGTGA CAGTGGTGGC AAACTTCGGG GAAACGGTGG TGGAGAAAGC AGTGATGGTA AGCTTCCAGA GTGGGTACCT CTTCATCCAG ACAGACAAGA CCATCTACAC CCCTGGCTCC ACTGTCTTAT ATCGGATCTT CACTGTGGAC AACAACCTAC TGCCCGTGGG CAAGACAGTC GTCATCCTCA TTGAGACCCC CGATGGCATT CCTGTCAAGA GAGACATTCT GTCTTCCAAC AACCAACACG GCATCTTGCC TTTGTCTTGG AACATTCCTG AACTGGTCAA CATGGGGCAG TGGAAGATCC GAGCCTTTTA CGAACATGCG CCGAAGCAGA TCTTCTCCGC AGAGTTTGAG GTGAAGGAAT ACGTGCTGCC CAGTTTTGAG GTCCGGGTGG AGCCCACAGA GACATTTTAT TACATCGATG ACCCAAATGG CCTGGAAGTT TCCATCATAG CCAAGTTCCT GTACGGGAAA AACGTGGACG GGACAGCCTT CGTGATTTTT GGGGTCCAGG ATGGCGATAA GAAGATTTCT CTGGCCCACT CCCTCACGCG CGTAGTGATT GAGGATGGTG TGGGGGATGC AGTGCTGACC CGGAAGGTGC TGATGGAGGG GGTACGGCCT TCCAACGCCG ACGCCCTGGT GGGGAAGTCC CTGTATGTCT CCGTCACTGT CATCCTGCAC TCAGGTAGTG ACATGGTAGA GGCAGAGCGC AGTGGGATCC CGATTGTCAC TTCCCCGTAC CAGATCCACT TCACCAAGAC ACCCAAATTC TTCAAGCCAG CCATGCCCTT TGACCTCATG GTGTTCGTGA CCAACCCCGA TGGCTCTCCG GCCAGCAAAG TGCTGGTGGT CACTCAGGGA TCTAATGCAA AGGCTCTCAC CCAAGATGAT GGCGTGGCCA AGCTAAGCAT CAACACACCC AACAGCCGCC AACCCCTGAC CATCACAGTC CGCACCAAGA AGGACACTCT CCCAGAATCA CGGCAGGCCA CCAAGACAAT GGAGGCCCAT CCCTACAGCA CTATGCACAA CTCCAACAAC TACCTACACT TGTCAGTGTC ACGAATGGAG CTCAAGCCGG GGGACAACCT CAATGTCAAC TTCCACCTGC GCACAGACCC AGGCCATGAG GCCAAGATCC GATACTACAC CTACCTGGTT ATGAACAAGG GGAAGCTCCT GAAGGCAGGC CGCCAGGTTC GGGAGCCTGG CCAGGACCTG GTGGTCTTGT CCCTGCCCAT CACTCCAGAG TTTATTCCTT CATTTCGCCT GGTGGCTTAC TACACCCTGA TTGGAGCTAG TGGCCAGAGG GAGGTGGTGG CTGACTCTGT GTGGGTGGAT GTGAAGGATT CCTGTATTGG CACGCTGGTG GTGAAGGGTG ACCCAAGAGA TAACCATCTC GCACCTGGGC AACAAACGAC ACTCAGGATT GAAGGAAACC AGGGGGCCCG AGTGGGGCTA GTGGCTGTGG ACAAGGGAGT GTTTGTGCTG AACAAGAAGA ACAAACTCAC ACAGAGCAAG ATCTGGGATG TGGTAGAGAA GGCAGACATT GGCTGCACCC CAGGCAGTGG GAAGAACTAT GCTGGTGTCT TCATGGATGC AGGCCTGGCC TTCAAGACAA GCCAAGGACT GCAGACTGAA CAGAGAGCAG ATCTTGAGTG CACCAAGCCA GCAGCCCGCC GCCGTCGCTC AGTACAGTTG ATGGAAAGAA GGATGGACAA AGCTGGTCAG TACACTGACA AGGGTCTTCG GAAGTGTTGT GAGGATGGTA TGCGGGATAT CCCTATGAGA TACAGCTGCC AGCGCCGGGC ACGCCTCATC ACCCAGGGCG AGAACTGCAT AAAGGCCTTC ATAGACTGCT GCAACCACAT CACCAAGCTG CGTGAACAAC ACAGAAGAGA CCACGTGCTG GGCCTGGCCA GGAGTGAATT GGAGGAAGAC ATAATTCCAG AAGAAGATAT TATCTCTAGA AGCCACTTCC CACAGAGCTG GTTGTGGACC ATAGAAGAGT TGAAAGAACC AGAGAAAAAT GGAATCTCTA CGAAGGTCAT GAACATCTTT CTCAAAGATT CCATCACCAC CTGGGAGATT CTGGCAGTGA GCTTGTCAGA CAAGAAAGGG ATCTGTGTGG CAGACCCCTA TGAGATCAGA GTGATGCAGG ACTTCTTCAT TGACCTGCGG CTGCCCTACT CTGTAGTGCG CAACGAACAG GTGGAGATCA GAGCTGTGCT CTTCAACTAC CGTGAACAGG AGGAACTTAA GGTGAGGGTG GAACTGTTGC ATAATCCAGC CTTCTGCAGC ATGGCCACCG CCAAGAATCG CTACTTCCAG ACCATCAAAA TCCCTCCCAA GTCCTCGGTG GCTGTACCGT ATGTCATTGT CCCCTTGAAG ATCGGCCAAC AAGAGGTGGA GGTCAAGGCT GCTGTCTTCA ATCACTTCAT CAGTGATGGT GTCAAGAAGA CACTGAAGGT CGTGCCAGAA GGAATGAGAA TCAACAAAAC TGTGGCCATC CATACACTGG ACCCAGAGAA GCTCGGTCAA GGGGGAGTGC AGAAGGTGGA TGTGCCTGCC GCAGACCTTA GCGACCAAGT GCCAGACACA GACTCTGAGA CCAGAATTAT CCTGCAAGGG AGCCCGGTGG TTCAGATGGC TGAAGATGCT GTGGACGGGG AGCGGCTGAA ACACCTGATC GTGACCCCCG CAGGCTGTGG GGAACAGAAC ATGATTGGCA TGACACCAAC AGTCATTGCG GTACACTACC TGGACCAGAC CGAACAGTGG GAGAAGTTCG GCATAGAGAA GAGGCAAGAG GCCCTGGAGC TCATCAAGAA AGGGTACACC CAGCAGCTGG CCTTCAAACA GCCCAGCTCT GCCTATGCTG CCTTCAACAA CCGGCCCCCC AGCACCTGGC TGACAGCCTA CGTGGTCAAG GTCTTCTCTC TAGCTGCCAA CCTCATCGCC ATCGACTCTC ACGTCCTGTG TGGGGCTGTT AAATGGTTGA TTCTGGAGAA ACAGAAGCCG GATGGTGTCT TTCAGGAGGA TGGGCCCGTG ATTCACCAAG AAATGATTGG TGGCTTCCGG AACGCCAAGG AGGCAGATGT GTCACTCACA GCCTTCGTCC TCATCGCACT GCAGGAAGCC AGGGACATCT GTGAGGGGCA GGTCAATAGC CTTCCTGGGA GCATCAACAA GGCAGGGGAG TATATTGAAG CCAGTTACAT GAACCTGCAG AGACCATACA CAGTGGCCAT TGCTGGGTAT GCCCTGGCCC TGATGAACAA ACTGGAGGAA CCTTACCTCG GCAAGTTTCT GAACACAGCC AAAGATCGGA ACCGCTGGGA GGAGCCTGAC CAGCAGCTCT ACAACGTAGA GGCCACATCC TACGCCCTCC TGGCCCTGCT GCTGCTGAAA GACTTTGACT CTGTGCCCCC TGTAGTGCGC TGGCTCAATG AGCAAAGATA CTACGGAGGC GGCTATGGCT CCACCCAGGC TACCTTCATG GTATTCCAAG CCTTGGCCCA ATATCAAACA GATGTCCCTG ACCATAAGGA CTTGAACATG GATGTGTCCT TCCACCTCCC CAGCCGTAGC TCTGCAACCA CGTTTCGCCT GCTCTGGGAA AATGGCAACC TCCTGCGATC GGAAGAGACC AAGCAAAATG AGGCCTTCTC TCTAACAGCC AAAGGAAAAG GCCGAGGCAC ATTGTCGGTG GTGGCAGTGT ATCATGCCAA ACTCAAAAGC AAAGTCACCT GCAAGAAGTT TGACCTCAGG GTCAGCATAA GACCAGCCCC TGAGACAGCC AAGAAGCCCG AGGAAGCCAA GAATACCATG TTCCTTGAAA TCTGCACCAA GTACTTGGGA GATGTGGACG CCACTATGTC CATCCTGGAC ATCTCCATGA TGACTGGCTT TGCTCCAGAC ACAAAGGACC TGGAACTGCT GGCCTCTGGA GTAGATAGAT ACATCTCCAA GTACGAGATG AACAAAGCCT TCTCCAACAA GAACACCCTC ATCATCTACC TAGAAAAGAT TTCACACACC GAAGAAGACT GCCTGACCTT CAAAGTTCAC CAGTACTTTA ATGTGGGACT TATCCAGCCC GGGTCGGTCA AGGTCTACTC CTATTACAAC CTCGAGGAAT CATGCACCCG GTTCTATCAT CCAGAGAAGG ACGATGGGAT GCTCAGCAAG CTGTGCCACA GTGAAATGTG CCGGTGTGCT GAAGAGAACT GCTTCATGCA ACAGTCACAG GAGAAGATCA ACCTGAATGT CCGGCTAGAC AAGGCTTGTG AGCCCGGAGT CGACTATGTG TACAAGACCG AGCTAACCAA CATAGAGCTG TTGGATGATT TTGATGAGTA CACCATGACC ATCCAGCAGG TCATCAAGTC AGGCTCAGAT GAGGTGCAGG CAGGGCAGCA ACGCAAGTTC ATCAGCCACA TCAAGTGCAG AAACGCCCTG AAGCTGCAGA AAGGGAAGAA GTACCTCATG TGGGGCCTCT CCTCTGACCT CTGGGGAGAA AAGCCCAACA CCAGCTACAT CATTGGGAAG GACACGTGGG TGGAGCACTG GCCTGAGGCA GAAGAATGCC AGGATCAGAA GTACCAGAAA CAGTGCGAAG AACTTGGGGC ATTCACAGAA TCTATGGTGG TTTATGGTTG TCCCAACACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT
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