pECMV-Slc6a4-m-FLAG
$84.00
In stock
SKU
PL85222
100μg liquid plasmid(Instantly):$105
| name | pECMV-Slc6a4-m-FLAG | another name | apECMV-Slc6a4-m-FLAG |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Kan |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | pFLAG-CMV-R：GCACTGGAGTGGCAACTT | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ATGCCTTCAG GGCAGGAGAG GCACTGGGGA GGGGTCACAG GGATGCCACC CGGGATCTGT TCAGGAAACA GCTATGACCG CGGCCGGCCG TTTAAACCTT ATCGTCGTCA TCCTTGTAAT CCAGGATATC ATTTGCTGCC AGATCCTCTT CTGAGATGAG TTTCTGCTCG AGCGGCCGCG TACGCGTCAC AGCATTCATG CGGATGTCCC CACACGGAAT TTCCGTTGGT GTTTCAGGAG TGATACTTTT AATAATGCGC TCCTTAAGTG TCCCTGGAGT GCTGATCAGC CGATAAATGA TGTATATAGG GATGCAGATG ACAGACGATG TTCCTATGCA GTAGCCCAAG ATGATACTCC AGTGGGGATA ATTGTATTGG AAAAGCCGGA GTTGGGGTGG ACTCATCAAA AAACTGCAAA TGATGAACAG GAGAAACAGA GGGCTGATGG CCACCCAGCA GATCCTCCAA AACCATCCGG GGCTGAAGCC CAGCATTTCC TTCACGTCGC TGCAGAACTG AGTGATTCCA TAGAACCAAG ACACGACGAC GGCCTCGATG AGAGCCACGG TGAGCACTGC TGGCCCCGTG GCGTACTCCT CCAGCAGGGT CACCACATAC GCCCCTCCTG ATGTCAGTGT GAGCAGGGAT CCCAAGATGC AAGTGATGAC CACGATGAGC ACAAACCATT CCCTGCGCTT GGCCCAGATG TGAGGAAACT CATCCAACAC AGCTGTGATC ACACCTTCCA GGCCTGCGAA CGTACTATCC AAACCCAGCG TGATTAACAT GAGGAAGAAG ATGATGGCAA AGAATGTGGA TGCTGGCATG TTAGCTATTG CCTCCGCATA TGTGATGAAA AGGAGGCTGG GGCCCGCGTC TTTGGCCACC TCGGACACGT CTTCGTTCCT CATCTCAGCC ATGTAGCCAA GCACCGTGAA GATGACAAAG CCAGAGACGA AGCTCGTCAT GCAGTTCACC ACACTGGTCA CCAGGGCATC TTGGTAACAG TTGTTGTTGA ACTTGTTGTA GCTAGCAAAC GCCAGGAGAA CCCCAAACCC CGGGCCAAGA GAGAAAAAGA TCTGAGCCGC AGCATCAACC CACACCCCTG TCTCCAAGAG TTTCTGCCAG TTGGGTTTCA AGTAAAAGAC AACCCCTCTC CAGGCTCCAG GAAGGGTGGC TCCCCTCACC AGCAGGACAG AAAGGACAAT GTAAGGGAAG GTGGCTGTCA CCCACACCAC CTTGCCAGAC GTTTTGACTC CTTTCCAGAT GCTGAAGTAG ATAATGGTGA AGATGAGCAT GATGCAGAGA GCCAGCTGCC AGCTGATGGT CCCCAGGTCC TGGAGTCCCT TTGACTGATG GATCTGCAGG ACATGGCGCA AGTAAAACTC CTCAGCAGGT GACGTGGAAT GGAGTGTCCA GGTGATGTTG TCCTGGGCGA AGTAGTTGGT GCAGTTGCCA GTGTTCCAAG AGTTCTTGCA GCTGGTCCAG GGCAGCTGGT CCGTGAAGGA GGAGATGAGG TAGTAGAGCG CCCAGGCTAT GATGGTGTTA TAGTAGGAGG CGATATAAAA GGCAATGATG CAGATGGCAT AGCCAATGCC TTTGAAAATC GGGCAGATCT TCTTCCATAT AGAAATGCAC CCATTTCGGT GGTACTGGCC CAGGGCGAGC TCCATGTAGA AGAGTGGGAT CCCCCCAAAG ATGGCCATGA TGGTGTAAGG GAGGAGGAAG GCCCCTCCAC CATTCTGGTA GCATATGTAG GGAAAACGCC AGATGTTGCC CAGGTCCACG GCATAGCCAA TGACAGACAG GAGGAAATCC ATCTTCTTGC CCCAGGTCTC CCGTTCCCCT TGGTGAATCT CAGCCACCAG GGTGGTGGTG GCAGCTGGCG TAGAGTGTGG CGCTTCATCC CCAGCGCTTG TACTGGGAAC TGCGGAGTAC CCATTGGATA TTTGTCCAGG CCCTGCCTTG TCTGCTGGTG TGGGGACACC CTTCTGCAGA ACACCATTTT CTTGGCAGTC CTCTTTGTCC TTACACTCTG ACAGCACTTT CTGAGAATTC AAAGGTGTGG TCTCCATGGC GATCGCGGCG GCAGATCTCC TCGGTACCGG ATCCAGTCGA CGAATTCCCG GCCGCCCTAT AGTGAGTCGT ATTACAAAAT TCTGACGGTT CACTAAACGA GCTCTCTATA GCTCGACCCA
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