pECMV-Celsr2-m-FLAG
$84.00
In stock
SKU
PL85216
100μg liquid plasmid(Instantly):$105
| name | pECMV-Celsr2-m-FLAG | another name | apECMV-Celsr2-m-FLAG |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Kan |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | pFLAG-CMV-R：GCACTGGAGTGGCAACTT | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GCGGAGCCGG GCTGCCAGCG CCCCCCTCCC AACGCCGCTG CTGCCGCTGC TACTGCTGTT GCTGCTGCTG CCACCGTCGC CACTACTGGG AGATCAAGTG GGGCCCTGTC GTTCTCTGGG ATCCGGGGGA CGCAGCTCCT CTGGGGCCTG CGCCCCCGTG GGCTGGCTCT GCCCAGCCTC TGCTTCGAAC CTCTGGCTCT ACACCAGCCG CTGCAGAGAA TCGGGCATTG AGCTGACCGG CCACCTGGTG CCCCACCACG ATGGCCTGAG GGTTTGGTGT CCAGAATCAG GGGCTCATAT CCCTCTTCCG CCATCCTCTG AAGGCTGCCC CTGGAGCTGT CGTCTCCTGG GTATCGGAGG ACACCTTTCT CCACAAGGCA CGCTGACCCT GCCTGAAGAG CACCCTTGCT TAAAGGCCCC ACGGCTCAGA TGCCAGTCTT GCAAGCTGGC ACAGGCCCCA GGGCTCAGGG CTGGGGAAGG CTCACCAGAG GAATCTCTGG GTGGGCGCAG GAAAAGGAAT GTGAATACAG CTCCCCAGTT CCAGCCTCCC AGCTACCAGG CCACAGTGCC TGAGAACCAG CCTGCTGGTA CCTCTGTTGC ATCCTTAAGA GCCATTGATC CAGATGAGGG TGAGGCGGGT CGACTTGAGT ACACCATGGA TGCCCTCTTT GATAGCCGCT CCAATCATTT CTTCTCCTTG GACCCAATCA CCGGTGTTGT CACCACAGCT GAGGAGCTGG ATCGGGAGAC CAAGAGCACC CACGTCTTCA GGGTCACTGC ACAGGATCAC GGTATGCCCC GACGGAGTGC CTTGGCCACG CTTACCATCT TGGTGACCGA CACCAACGAT CACGACCCTG TTTTTGAGCA GCAAGAGTAC AAGGAGAGCC TCAGGGAGAA CCTGGAGGTT GGCTATGAGG TGCTTACAGT CAGGGCCACC GACGGCGACG CCCCTCCCAA TGCCAACATT CTGTACCGCC TGCTGGAGGG GGCCGGAGGC AGCCCCTCAG ACGCCTTTGA GATCGATCCT CGCTCGGGGG TGATCCGAAC CCGCGGCCCT GTAGACCGGG AGGAAGTGGA ATCCTACAAG TTAACAGTGG AGGCGAGTGA CCAGGGCCGG GACCCAGGCC CGCGGAGTTC CACAGCCATT GTTTTCCTGT CGGTGGAGGA TGATAATGAC AACGCCCCCC AGTTTAGCGA GAAGCGTTAT GTGGTCCAGG TGCGGGAGGA TGTGACCCCA GGAGCCCCAG TTCTCCGAGT CACCGCCTCC GATAGAGACA AGGGCAGCAA TGCCCTAGTG CACTACAGCA TCATGAGTGG CAATGCTCGG GGCCAGTTCT ATCTGGATGC TCAGACTGGA GCCCTGGATG TGGTAAGCCC ACTCGACTAT GAGACAACCA AAGAGTATAC GCTTCGGATC CGGGCTCAGG ACGGTGGCCG TCCTCCACTC TCCAATGTCT CTGGCCTAGT AACAGTGCAA GTCCTAGACA TCAACGATAA CGCCCCCATC TTTGTCAGCA CCCCTTTCCA GGCCACCGTC CTAGAGAGTG TCCCTTTAGG CTACCTTGTT CTGCACGTCC AGGCAATTGA TGCTGACGCT GGTGATAACG CCCGCCTCGA GTATAGCCTG GCAGGGGTTG GGCACGACTT TCCCTTCACC ATTAACAATG GCACAGGCTG GATCTCTGTG GCCGCAGAGT TGGATCGGGA AGAGGTTGAT TTCTACAGCT TTGGTGTAGA GGCCCGGGAC CATGGCACCC CAGCGCTCAC GGCCTCAGCC AGTGTCAGTG TAACCATCCT GGACGTCAAT GACAACAACC CAACCTTCAC GCAGCCAGAG TACACCGTTC GGCTCAACGA GGACGCCGCC GTGGGCACCA GTGTGGTGAC CGTGTCAGCC GTGGATCGAG ACGCTCACAG TGTCATTACC TACCAGATCA CCAGCGGCAA CACCCGCAAC CGCTTCTCTA TCACCAGCCA AAGTGGTGGT GGACTGGTCT CCCTTGCCTT ACCGCTAGAC TACAAACTCG AGCGGCAGTA TGTGCTGGCA GTGACTGCCT CAGATGGCAC AAGGCAGGAC ACAGCTCAGA TAGTAGTGAA TGTCACCGAT GCCAACACCC ACCGTCCCGT CTTCCAGAGC TCCCACTACA CAGTCAACGT TAATGAAGAC CGGCCAGCAG GCACCACAGT GGTGCTGATC AGCGCCACGG ATGAGGACAC GGGGGAGAAT GCCCGAATCA CCTACTTTAT GGAGGATAGC ATCCCCCAGT TCCGAATCGA CGCGGACACT GGGGCTGTCA CCACCCAGGC TGAGCTGGAC TACGAGGACC AGGTGTCTTA TACCCTGGCC ATCACTGCTC GGGACAATGG CATTCCCCAG AAGTCTGACA CCACCTATTT GGAGATCCTG GTGAATGACG TGAACGACAA CGCCCCCCAG TTCCTCAGAG ATTCCTACCA GGGCAGCGTC TACGAGGACG TGCCGCCCTT CACCAGCGTC CTGCAGATCT CAGCCACGGA TCGAGACTCC GGTCTGAATG GCAGGGTTTT CTACACCTTC CAAGGAGGAG ATGATGGGGA CGGTGACTTT ATTGTAGAGT CGACATCGGG CATTGTGCGC ACACTGCGGA GGCTGGATCG TGAGAACGTG GCCCAGTACG TCCTGCGGGC CTATGCGGTG GACAAGGGCA TGCCACCAGC CCGCACACCC ATGGAAGTGA CAGTTACTGT CCTGGATGTG AATGACAACC CCCCTGTCTT TGAACAGGAT GAGTTTGACG TATTTGTGGA AGAGAACAGC CCCATCGGGC TGGCCGTGGC CCGGGTCACA GCCACTGACC CAGATGAAGG GACCAACGCA CAGATCATGT ACCAGATTGT GGAGGGCAAT ATCCCTGAGG TCTTTCAGTT GGATATATTC TCTGGCGAGC TAACTGCCCT GGTAGATTTG GACTATGAGG ACCGGCCTGA ATATGTCCTG GTCATCCAGG CTACGTCTGC TCCCTTGGTG AGCAGGGCTA CTGTCCATGT CCGCCTCCTT GACCGCAATG ATAACCCGCC CGTGCTGGGC AACTTTGAGA TTCTCTTTAA CAACTATGTC ACCAACCGCT CCAGCAGCTT CCCCGGTGGT GCCATAGGCC GTGTGCCTGC CCACGACCCC GATATCTCAG ACAGCCTGAC GTACAGCTTC GAGCGAGGAA ATGAACTCAG CTTGGTCCTG CTCAATGCCT CCACCGGTGA GCTGAGACTG AGCCGGGCAC TGGACAACAA CCGGCCCCTG GAAGCCATCA TGAGTGTGCT GGTGTCAGAT GGTGTCCACA GTGTGACAGC CCAGTGCTCG CTACGTGTCA CCATCATCAC GGACGAGATG CTCACACACA GCATCACGCT GCGCTTGGAA GACATGTCTC CAGAACGCTT TCTCTCACCA CTGCTGGGAC TCTTCATTCA GGCCGTGGCA GCCACGTTGG CCACACCCCC GGATCATGTG GTGGTCTTCA ATGTGCAAAG GGATACTGAT GCCCCAGGCG GCCATATCCT CAACGTAAGC CTGTCGGTGG GCCAGCCTCC AGGACCCGGG GGTGGGCCAC CCTTTCTGCC TTCAGAGGAT CTCCAGGAGC GCCTGTACCT CAACCGCAGC CTGCTCACCG CCATCTCGGC GCAGCGCGTG CTCCCCTTCG ACGACAACAT TTGCCTGCGG GAGCCCTGCG AGAATTACAT GCGATGTGTG TCTGTGCTGC GGTTCGACTC CTCTGCGCCC TTCATCGCTT CCTCTTCGGT GCTCTTCCGG CCCATCCACC CTGTCGGGGG TCTGCGCTGT CGCTGCCCAC CAGGCTTCAC AGGCGACTAC TGCGAGACCG AGGTGGACCT CTGTTACTCA AGACCTTGTG GACCCCATGG GCGCTGCCGC AGCCGAGAGG GTGGCTATAC CTGCCTCTGT CTGGATGGCT ACACGGGCGA GCACTGTGAA GCGAGTACCC ACTCAGGCCG TTGTACTCCA GGTGTCTGCA AGAACGGGGG TACCTGTGTC AACCTGTTGG TGGGAGGTTT CAAATGTGAC TGCCCCTCCG GGGACTTTGA GAAACCCTTC TGCCAGGTGA CCACACGCAG CTTCCCTGCC CGCTCCTTCA TCACCTTCCG TGGCCTGCGC CAGCGCTTCC ACTTCACCCT GGCCCTCTCG TTTGCTACCA AGGAGCGTAA CGGGCTACTG CTGTACAATG GGCGCTTCAA TGAGAAGCAC GACTTTGTGG CTCTCGAGGT GATCCAGGAA CAGGTGCAGC TCACCTTCTC TGCAGGGGAA TCGACCACCA CCGTGTCTCC GTTTGTGCCG GGAGGGGTCA GTGATGGCCA GTGGCACACA GTGCAGCTGA AGTACTATAA CAAGCCACTG TTGGGTCAGA CAGGGCTTCC CCAAGGTCCA TCTGAGCAGA AGGTAGCCGT GGTGTCCGTG GATGGCTGTG ACACAGGGGT GGCTCTGCGC TTTGGAGCTA TGCTGGGCAA CTACTCCTGT GCTGCCCAGG GCACCCAAGG AGGCAGCAAG AAGTCTCTGG ACCTGACAGG GCCCTTGTTG CTGGGTGGGG TGCCGGATCT GCCCGAGAGC TTCCCCGTCC GAATGCGGCA CTTTGTGGGC TGCATGAAGG ACCTCCAGGT GGATAGCCGG CACATCGACA TGGCCGACTT CATTGCCAAC AATGGCACTG TGCCTGGCTG CCCCACCAAG AAGATCGTGT GCGACAGCAG CATTTGCCAC AACGGTGGCA CCTGTGTGAA CCAGTGGAAT GCGTTCAGCT GCGAGTGTCC ACTAGGCTTC GGGGGCAAGA GCTGCGCCCA GGAAATGGCC AACCCCCAGC GTTTCCTGGG CAGCAGCCTT GTGGCCTGGC ATGGCCTCTC TCTGCCCATC TCTCAGCCCT GGCACCTCAG CCTCATGTTC CGCACACGCC AGGCAGATGG CGTCCTGCTG CAGGCCGTCA CCAGGGGGCG CAGCACCATC ACCCTGCAGC TTCGGGCAGG CCACGTAGTG CTAAGTGTGG AGGGCACAGG GCTCCAGGCG TCATCTCTGC GTCTTGAGCC AGGCCGAGCC AATGATGGTG ACTGGCATCA TGCACAGCTG GCACTGGGAG CCAGCGGGGG CCCTGGCCAT GCCATTCTGT CCTTTGACTA TGGGCAACAG AAGGCAGAGG GTAATCTGGG CCCTCGGCTG CATGGACTGC ACCTGAGCAA TATTACAGTC GGGGGAGTTC CTGGGCCAGC CAGCGGAGTG GCCCGTGGCT TCCGGGGCTG TTTGCAGGGT GTCAGGGTAA GCGAGACACC TGAGGGTATC AGCAGTCTAG ATCCCAGCCG CGGGGAGAGC ATCAATGTGG AGCCAGGCTG TAGCTGGCCA GATCCCTGTG ACTCGAATCC ATGCCCTACC AACAGCTACT GCAGCAATGA CTGGGACAGC TATTCTTGTA GCTGTGTTCT AGGTTACTAT GGTGACAACT GTACAAATGT GTGTGACCTG AACCCATGCG AGCACCAGTC CGTGTGTACC CGAAAACCCA ATACACCCCA CGGCTACATC TGCGAGTGTT TACCAAATTA CCTTGGGCCA TATTGTGAGA CCAGGATTGA CCAACCTTGC CCCCGTGGCT GGTGGGGACA CCCCACATGT GGTCCATGCA ACTGTGATGT CAGCAAAGGC TTTGACCCAG ATTGCAACAA GACAAGTGGG GAGTGCCACT GCAAGGAGAA TCACTACCGG CCCCCCGGCA GCCCCACTTG TCTCTTGTGT GACTGTTACC CCACTGGTTC TTTGTCCCGA GTCTGTGACC CCGAGGACGG CCAGTGTCCG TGCAAGCCTG GAGTCATTGG GCGTCAGTGT GACCGCTGTG ACAACCCTTT TGCTGAGGTC ACCACCAATG GCTGTGAAGT GAATTACGAC AGCTGCCCAC GGGCCATAGA GGCTGGGATC TGGTGGCCCC GCACGCGGTT CGGGCTACCT GCTGCTGCCC CCTGCCCCAA AGGCTCCTTT GGGACTGCTG TGCGCCACTG TGATGAGCAC AGGGGTTGGC TCCCCCCAAA CCTCTTCAAC TGCACGTCAG TCACCTTCTC AGAGCTAAAG GGCTTCGCTG AGCGGCTGCA GAGGAACGAA TCAGGCCTGG ACTCAGGACG CTCCCAGAGG CTAGCCCTGC TCCTGCGCAA TGCCACCCAG CACACCTCTG GCTACTTCGG CAGTGATGTC AAGGTGGCCT ACCAGCTGGC CACACGGCTC TTGGCTCATG AGAGTGCCCA GCGGGGCTTT GGGCTATCCG CCACACAGGA TGTACACTTC ACGGAGAATC TGCTGAGGGT GGGCAGCGCC CTCCTGGATG CAGCCAACAA GAGGCACTGG GAACTGATCC AGCAGACAGA GGGTGGCACC GCCTGGCTGC TCCAGCACTA TGAGGCTTAC GCCAGCGCCC TTGCCCAGAA TATGCGGCAC ACCTACCTAA GCCCCTTCAC CATCGTCACA CCCAACATTG TCATCTCTGT AGTGCGCCTG GATAAGGGGA ACTTTGCTGG GACCAAGCTG CCCCGTTATG AGGCATTACG TGGGGAGCGC CCCCCAGATC TTGAGACCAC AGTCATTTTG CCAGAATCTG TCTTCAGAGA GATGCCCTCC ATGGTGAGGT CTGCAGGACC TGGTGAAGCC CAGGAGACGG AGGAGCTGGC CCGGCGGCAG CGGAGGCACC CAGAACTGAG TCAGGGAGAG GCAGTGGCCA GTGTCATCAT CTACCACACC CTGGCTGGAC TGCTGCCCCA CAACTATGAC CCCGATAAGC GTAGCCTGAG AGTCCCCAAG CGCCCCGTCA TCAACACCCC TGTGGTGAGC ATCAGCGTTC ACGACGACGA GGAACTCCTG CCGCGGGCTC TGGACAAGCC AGTCACCGTG CAGTTCCGGC TGCTGGAGAC CGAGGAGCGG ACCAAGCCCA TCTGTGTCTT CTGGAACCAT TCAATCCTGG TCAGTGGCAC TGGCGGCTGG TCCGCCCGAG GCTGCGAAGT CGTCTTCCGT AACGAGAGCC ACGTCAGCTG CCAGTGCAAC CATATGACAA GCTTTGCTGT GCTTATGGAT ATGTCACGAC GAGAGAACGG TGAGATCTTG CCACTGAAGA CCCTGACATA CGTGGCCCTT GGAGTCACCT TGGCTGCCCT GATGCTCACC TTCCTCTTCC TCACCCTCCT CCGTGCCCTC CGCTCCAACC AGCACGGCAT CCGACGCAAC CTCACAGCTG CCCTGGGCCT GGCCCAGCTG GTCTTTCTCC TGGGCATCAA CCAGGCTGAC CTCCCTTTTG CCTGTACAGT CATCGCTATC CTGCTGCATT TCCTGTACCT CTGCACCTTC TCCTGGGCTC TGCTGGAGGC CTTACACCTG TACCGCGCGC TCACAGAGGT GCGCGACGTC AATGCCAGTC CCATGCGTTT CTACTACATG CTGGGCTGGG GCGTCCCTGC TTTCATCACA GGCCTCGCTG TGGGCTTGGA TCCGGAAGGC TATGGGAACC CTGACTTCTG CTGGCTCTCT GTCTATGATA CCCTCATCTG GAGCTTTGCT GGACCAGTGG CCTTTGCTGT TTCGATGAGT GTCTTCCTGT ACATCCTGTC GGCCCGAGCC TCCTGTGCTG CCCAACGGCA GGGCTTCGAG AAGAAAGGGC CTGTCTCGGG CCTGCGCTCC TCCTTCACGG TCCTCCTGCT GCTGAGTGCC ACGTGGCTGC TGGCACTGCT CTCTGTCAAC AGTGACACTC TGCTCTTCCA CTACCTCTTC GCTGCCTGCA ATTGTGTCCA GGGCCCCTTC ATCTTCCTCT CCTACGTGGT GCTCAGCAAG GAGGTCCGGA AAGCACTCAA GTTCGCCTGC AGCCGGAAGC CCAGTCCCGA CCCTGCCTTG ACCACTAAGT CTACCCTGAC CTCGTCCTAT AACTGCCCCA GCCCCTACGC AGACGGGAGG CTGTACCAGC CTTACGGAGA TTCGGCTGGC TCGTTGCACA GTGCCAGCCG CTCCGGCAAG AGTCAGCCCA GCTACATCCC CTTCTTGCTG AGGGAGGAGT CCACGCTGAA CCCTGGCCAG GTTCCCCCTG GCCTAGGGGA CCCAAGCGGC CTGTTCTTGG AAGGCCAAGC CCAACAACAT GATCCCGACA CGGACTCTGA CAGTGACCTG TCCCTGGAAG ATGACCAGAG TGGTTCCTAT GCCTCCACCC ACTCATCAGA CAGCGAGGAA GAGGAGGAGG AGGCCGCCTT CCCTGGCGAG CAGGGCTGGG ACAGCCTGCT GGGTCCTGGA GCTGAGAGAC TGCCCCTTCA CAGTACCCCC AAGGATGGGG GTCCGGGTTC TGGGAAGGTC CCTTGGCTGG GAGACTTTGG GACCACAACA AAGGAGAATA GTGGTAGTGG GCCCCTTGAA GAGCGGCCAC GGGAGAATGG AGATGCCCTA ACTCGGGAAG GGTCTCTGGG ACCCCTTCCG GGCCCTTCTA CCCAACCTCA CAAAGGCATC CTCAAGAAGA AGTGTCTGCC CACCATCAGC GAAAAGAGCA GCCTCCTAAG GCTGCCCCTG GAGCAGGGCA CAGGGTCTTC TCGGGGCTCC TCCATCAGTG AGGGCAGTCG GCACGGACCT CCTCCCCGCC CACCTCCGCG CCAGTCTCTG CAGGAACAGC TGAATGGGGT CATGCCTGTC GCCATGAGCA TCAAGGCAGG CACGGTGGAT GAGGACTCCT CTGGCTCTGA ATTTCTCTTC TTTAACTTCC TGCATACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
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