pHP13-P43
$128.00
In stock
SKU
PL85215
100μg liquid plasmid(Instantly):$149
| name | pHP13-P43 | another name | apHP13-P43 |
| Insert Size (bp) | Plasmid host | Bacillus subtilis | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Empty vector | Prokaryotic resistance | Chl |
| Selective marker | Fluorescent labeling | ||
| Promoter | p43 | Replicon | rep60 |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TATATTTTAT AGGATTGGTT TTTAGGAAAT TTAAACTGCA ATATATCCTT GTTTAAAACT TGGAAATTAT CGTGATCAAC AAGTTTATTT TCTGTAGTTT TGCATAATTT ATGGTCTATT TCAATGGCAG TTACGAAATT ACACCTCTTT ACTAATTCAA GGGTAAAATG GCCTTTTCCT GAGCCGATTT CAAAGATATT ATCATGTTCA TTTAATCTTA TATTTGTCAT TATTTTATCT ATATTATGTT TTGAAGTAAT AAAGTTTTGA CTGTGTTTTA TATTTTTCTC GTTCATTATA ACCCTCTTTA ATTTGGTTAT ATGAATTTTG CTTATTAACG ATTCATTATA ACCACTTATT TTTTGTTTGG TTGATAATGA ACTGTGCTGA TTACAAAAAT ACTAAAAATG CCCATATTTT TTCCTCCTTA TAAAATTAGT ATAATTATAG CACGAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT ATGACCATGA TTACGCCAAG CTTGCGGCTT CCTTGTAGAG CTCAGCATTA TTGAGTGGAT GATTATATTC CTTTTGATAG GTGGTATGTT TTCGCTTGAA CTTTTAAATA CAGCCATTGA ACATACGGTT GATTTAATAA CTGACAAACA TCACCCTCTT GCTAAAGCGG CCAAGGACGC TGCCGCCGGG GCTGTTTGCG TTTTTGCCGT GATTTCGTGT ATCATTGGTT TACTTATTTT TTTGCCAAAG CTGTAATGGC TGAAAATTCT TACATTTATT TTACATTTTT AGAAATGGGC GTGAAAAAAA GCGCGCGATT ATGTAAAATA TAAAGTGATA GCGGTACCAT TATAGGTAAG AGAGGAATGT ACACATGCTG CAGGTCGACG GATCCCCGGG AATTCACTGG CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT AATCGCCTTG CAGCACATCC CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC GATCGCCCTT CCCAACAGTT GCGCAGCCTG AATGGCGAAT GGCGACTAAC GGGGCAGGTT AGTGACATTA GAAAACCGAC TGTAAAAAGT ACAGTCGGCA TTATCTCATA TTATAAAAGC CAGTCATTAG GCCTATCTGA CAATTCCTGA ATAGAGTTCA TAAACAATCC TGCATGATAA CCATCACAAA CAGAATGATG TACCTGTAAA GATAGCGGTA AATATATTGA ATTACCTTTA TTAATGAATT TTCCTGCTGT AATAATGGGT AGAAGGTAAT TACTATTATT ATTGATATTT AAGTTAAACC CAGTAAATGA AGTCCATGGA ATAATAGAAA GAGAAAAAGC ATTTTCAGGT ATAGGTGTTT TGGGAAACAA TTTCCCCGAA CCATTATATT TCTCTACATC AGAAAGGTAT AAATCATAAA ACTCTTTGAA GTCATTCTTT ACAGGAGTCC AAATACCAGA GAATGTTTTA GATACACCAT CAAAAATTGT ATAAAGTGGC TCTAACTTAT CCCAATAACC TAACTCTCCG TCGCTATTGT AACCAGTTCT AAAAGCTGTA TTTGAGTTTA TCACCCTTGT CACTAAGAAA ATAAATGCAG GGTAAAATTT ATATCCTTCT TGTTTTATGT TTCGGTATAA AACACTAATA TCAATTTCTG TGGTTATACT AAAAGTCGTT TGTTGGTTCA AATAATGATT AAATATCTCT TTTCTCTTCC AATTGTCTAA ATCAATTTTA TTAAAGTTCA TTTGATATGC CTCCTAAATT TTTATCTAAA GTGAATTTAG GAGGCTTACT TGTCTGCTTT CTTCATTAGA ATCAATCCTT TTTTAAAAGT CAATATTACT GTAACATAAA TATATATTTT AAAAATATCC CACTTTATCC AATTTTCGTT TGTTGAACTA ATGGGTGCTT TAGTTGAAGA ATAAAAGACC ACATTAAAAA ATGTGGTCTT TTGTGTTTTT TTAAAGGATT TGAGCGTAGC GAAAAATCCT TTTCTTTCTT ATCTTGATAC TATATAGAAA CAACATCATT TTTCAAAATT AGGTCAAAGC CTTGTGTATC AAGGGTTTGA TGGTTCTTTG ACAGGTAAAA ACTCCTTCTG CTATTATTAA GGTGTCGAAT CAAAATAATA GAATGCTAGA GAACTAGCTC AGAAGGAGTT TTTTTGTTGA TTTATTCATC TGAAAATGAT TATAGCATCC TCGAAGATAA AACCGCAACA GGTAAAAAGC GGGATTGGAA GGGGAAAAAG AGACGGACGA ACCTCATGGC GGAGCATTAC GAAGCGTTAG AGAGTAAGAT TGGGGCACCT TACTATGGCA AAAAGGCTGA AAAACTAATT AGTTGTGCAG AGTATCTTTC GTTTAAGAGA GACCCGGAGA CGGGCAAGTT AAAACTGTAT CAAGCCCATT TTTGTAAAGT GAGGTTATGT CCGATGTGTG CGTGGCGCAG GTCGTTAAAA ATTGCTTATC ACAATAAGTT GATCGTAGAG GAAGCCAATA GACAGTACGG CTGCGGATGG ATTTTTCTCA CGCTGACGAT TCGAAATGTA AAGGGAGAAC GGCTGAAGCC ACAAATTTCT GCGATGATGG AAGGCTTTAG GAAACTGTTC CAGTACAAAA AAGTAAAAAC TTCGGTTCTT GGATTTTTCA GAGCTTTAGA GATTACCAAA AATCATGAAG AAGATACATA TCATCCTCAT TTTCATGTGT TGATACCAGT AAGGAAAAAT TATTTTGGGA AAAACTATAT TAAGCAGGCG GAGTGGACGA GCCTTTGGAA AAAGGCGATG AAATTGGATT ACACTCCAAT TGTCGATATT CGTCGAGTGA AAGGTAAAGC TAAGATTGAC GCTGAACAGA TTGAAAACGA TGTGCGGAAC GCAATGATGG AGCAAAAAGC TGTTCTCGAA ATCTCTAAAT ATCCGGTTAA GGATACGGAT GTTGTGCGCG GTAATAAGGT GACTGAAGAC AATCTGAACA CGGTGCTTTA CTTGGATGAT GCGTTGGCAG CTCGAAGGTT AATTGGATAC GGTGGCATTT TGAAGGAGAT ACATAAAGAG CTGAATCTTG GTGATGCGGA GGACGGCGAT CTGGTCAAGA TTGAGGAAGA AGATGACGAG GTTGCAAATG GTGCATTTGA GGTTATGGCT TATTGGCATC CTGGCATTAA AAATTACATA ATCAAATAAA AAAAGCAGAC CTTTAGAAGG CCTGCTTTTT TAACTAACCC ATTTGTATTG TGTTGAAATA TGTTTTGTAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGCG ATTCACAAAA AATAGGCACA CGAAAAACAA GTTAAGGGAT GCAGTTTATG CATCCCTTAA CTTACTTATT AAATAATTTA TAGCTATTGA AAAGAGATAA GAATTGTTCA AAGCTAATAT TGTTTAAATC GTCAATTCCT GCATGTTTTA AGGAATTGTT AAATTGATTT TTTGTAAATA TTTTCTTGTA TTCTTTGTTA ACCCATTTCA TAACGAAATA ATTATACTTT TGTTTATCTT TGTGTGATAT TCTTGATTTT TTTCTACTTA ATCTGATAAG TGAGCTATTC ACTTTAGGTT TAGGATGAAA ATATTCTCTT GGAACCATAC TTAATATAGA AATATCAACT TCTGCCATTA AAAGTAATGC CAATGAGCGT TTTGTATTTA ATAATCTTTT AGCAAACCCG TATTCCACGA TTAAATAAAT CTCATTAGCT ATACTATCAA AAACAATTTT GCGTATTATA TCCGTACTTA TGTTATAAGG TATATTACCA
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