pECMV-Crebbp-m-FLAG
$157.00
In stock
SKU
PL85208
100μg liquid plasmid(Instantly):$178
| name | pECMV-Crebbp-m-FLAG | another name | apECMV-Crebbp-m-FLAG |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Kan |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | pFLAG-CMV-R：GCACTGGAGTGGCAACTT | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGCCGAGAAC TTGCTGGACG GACCGCCCAA CCCCAAACGA GCCAAACTCA GCTCGCCCGG CTTCTCCGCG AATGACAACA CAGATTTTGG ATCATTGTTT GACTTGGAAA ATGACCTTCC TGATGAGCTG ATCCCCAATG GAGAATTAAG CCTTTTAAAC AGTGGGAACC TTGTTCCAGA TGCTGCGTCC AAACATAAAC AACTGTCAGA GCTTCTTAGA GGAGGCAGCG GCTCTAGCAT CAACCCAGGG ATAGGCAATG TGAGTGCCAG CAGCCCTGTG CAACAGGGCC TTGGTGGCCA GGCTCAGGGG CAGCCGAACA GTACAAACAT GGCCAGCTTA GGTGCCATGG GCAAGAGCCC TCTGAACCAA GGAGACTCAT CAACACCCAA CCTGCCCAAA CAGGCAGCCA GCACCTCTGG GCCCACTCCC CCTGCCTCCC AAGCACTGAA TCCACAAGCA CAAAAGCAAG TAGGGCTGGT GACCAGTAGT CCTGCCACAT CACAGACTGG ACCTGGGATC TGCATGAATG CTAACTTCAA CCAGACCCAC CCAGGCCTTC TCAATAGTAA CTCTGGCCAT AGCTTAATGA ATCAGGCTCA ACAAGGGCAA GCTCAAGTCA TGAATGGATC TCTTGGGGCT GCTGGAAGAG GAAGGGGAGC TGGAATGCCC TACCCTGCTC CAGCCATGCA GGGGGCCACA AGCAGTGTGC TGGCGGAGAC CTTGACACAG GTTTCCCCAC AAATGGCTGG CCATGCTGGA CTAAATACAG CACAGGCAGG AGGCATGACC AAGATGGGAA TGACTGGTAC CACAAGTCCA TTTGGACAAC CCTTTAGTCA AACTGGAGGG CAGCAGATGG GAGCCACTGG AGTGAACCCC CAGTTAGCCA GCAAACAGAG CATGGTCAAT AGTTTACCTG CTTTTCCTAC AGATATCAAG AATACTTCAG TCACCACTGT GCCAAATATG TCCCAGTTGC AAACATCAGT GGGAATTGTA CCCACACAAG CAATTGCAAC AGGCCCCACA GCAGACCCTG AAAAACGCAA ACTGATACAG CAGCAGCTGG TTCTACTGCT TCATGCCCAC AAATGTCAGA GACGAGAGCA AGCAAATGGA GAGGTTCGAG CCTGTTCTCT CCCACACTGT CGAACCATGA AAAACGTTTT GAATCACATG ACACATTGTC AGGCTGGGAA AGCCTGCCAA GTTGCCCATT GTGCATCTTC ACGACAAATC ATCTCTCATT GGAAGAACTG CACACGACAT GACTGTCCTG TTTGCCTCCC TTTGAAAAAT GCCAGTGACA AGCGAAACCA ACAAACCATC CTGGGATCTC CAGCTAGTGG AATTCAAAAC ACAATTGGTT CTGTTGGTGC AGGGCAACAG AATGCCACTT CCTTAAGTAA CCCAAATCCC ATAGACCCCA GTTCCATGCA GCGGGCCTAT GCTGCTCTAG GACTCCCCTA CATGAACCAG CCTCAGACGC AGCTGCAGCC TCAGGTTCCT GGCCAGCAAC CAGCACAGCC TCCAGCCCAC CAGCAGATGA GGACTCTCAA TGCCCTAGGA AACAACCCCA TGAGTATCCC AGCAGGAGGA ATAACAACAG ATCAACAGCC ACCAAACTTG ATTTCAGAAT CAGCTCTTCC AACTTCCTTG GGGGCTACCA ATCCACTGAT GAATGATGGT TCAAACTCTG GTAACATTGG AAGCCTCAGC ACGATACCTA CAGCAGCGCC TCCTTCCAGC ACTGGTGTTC GAAAAGGCTG GCATGAACAT GTGACTCAGG ACCTACGGAG TCATCTAGTC CATAAACTCG TTCAAGCCAT CTTCCCAACT CCAGACCCTG CAGCTCTGAA AGATCGCCGC ATGGAGAACC TGGTTGCCTA TGCTAAGAAA GTGGAGGGAG ACATGTATGA GTCTGCTAAT AGCAGGGATG AATACTATCA TTTATTAGCA GAGAAAATCT ATAAAATACA AAAAGAACTA GAAGAAAAGC GGAGGTCACG TTTACATAAG CAAGGCATCC TGGGTAACCA GCCAGCTTTA CCAGCTTCTG GGGCTCAGCC CCCTGTGATT CCACCAGCCC AGTCTGTAAG ACCTCCAAAT GGGCCCCTGC CTTTGCCAGT GAATCGCATG CAGGTTTCTC AAGGGATGAA TTCATTTAAC CCAATGTCCC TGGGAAACGT CCAGTTGCCA CAGGCACCCA TGGGACCTCG TGCAGCCTCC CCTATGAACC ACTCTGTGCA GATGAACAGC ATGGCCTCAG TTCCGGGTAT GGCCATTTCT CCTTCACGGA TGCCTCAGCC TCCAAATATG ATGGGCACTC ATGCCAACAA CATTATGGCC CAGGCACCTA CTCAGAACCA GTTTCTGCCA CAGAACCAGT TTCCATCATC CAGTGGGGCA ATGAGTGTGA ACAGTGTGGG CATGGGGCAA CCAGCAGCCC AGGCAGGTGT TTCACAGGGT CAGGTACCTG GAGCTGCTCT CCCTAACCCT CTGAACATGC TGGCACCCCA GGCCAGCCAG CTGCCTTGCC CACCAGTGAC ACAGTCACCA TTGCACCCGA CTCCACCTCC TGCTTCCACA GCTGCTGGCA TGCCCTCTCT CCAACATCCA ACGGCACCAG GAATGACCCC TCCTCAGCCA GCAGCTCCCA CTCAGCCATC TACTCCTGTG TCATCTGGGC AGACTCCTAC CCCAACTCCT GGCTCAGTGC CCAGCGCTGC CCAAACACAG AGTACCCCTA CAGTCCAGGC AGCAGCACAG GCTCAGGTGA CTCCACAGCC TCAGACCCCA GTGCAGCCAC CATCTGTGGC TACTCCTCAG TCATCACAGC AGCAACCAAC GCCTGTGCAT ACTCAGCCTC CTGGCACACC GCTTTCTCAG GCAGCAGCCA GCATTGATAA TAGAGTCCCT ACTCCCTCCT CTGTGACCAG TGCTGAAACC AGTTCCCAGC AGCCAGGACC CGATGTGCCC ATGCTGGAAA TGAAGACAGA GGTGCAGACA GATGATGCTG AGCCTGAACC TACTGAATCC AAGGGGGAAC CTCGGTCTGA GATGATGGAA GAGGATTTAC AAGGTTCTTC CCAAGTAAAA GAAGAGACAG ATACGACAGA GCAGAAGTCA GAGCCAATGG AAGTAGAAGA AAAGAAACCT GAAGTAAAAG TGGAAGCTAA AGAGGAAGAA GAGAACAGTT CGAACGACAC AGCCTCACAA TCAACATCTC CTTCCCAGCC ACGCAAAAAA ATCTTTAAAC CCGAGGAGCT ACGCCAGGCA CTTATGCCAA CTCTAGAAGC ACTCTATCGA CAGGACCCAG AGTCTTTGCC TTTTCGTCAG CCTGTAGATC CTCAGCTCCT AGGAATCCCA GATTATTTTG ATATAGTGAA GAATCCTATG GACCTTTCTA CCATCAAACG AAAGCTGGAC ACAGGGCAAT ATCAAGAACC CTGGCAGTAT GTGGATGATG TCTGGCTTAT GTTCAACAAT GCGTGGCTAT ATAATCGTAA AACGTCCCGT GTATATAAAT TTTGCAGTAA ACTTGCAGAG GTCTTTGAAC AAGAAATTGA CCCTGTCATG CAGTCTCTTG GATATTGCTG TGGACGAAAG TATGAGTTCT CCCCACAGAC TTTGTGCTGT TACGGAAAGC AGCTGTGTAC AATTCCTCGT GATGCAGCCT ACTACAGCTA TCAGAATAGG TATCATTTCT GTGAGAAGTG TTTCACAGAG ATCCAGGGCG AGAATGTGAC CCTGGGTGAC GACCCTTCCC AACCTCAGAC GACAATTTCC AAGGATCAAT TTGAAAAGAA GAAAAATGAT ACCTTAGATC CTGAACCTTT TGTTGACTGC AAAGAGTGTG GCCGGAAGAT GCATCAGATT TGTGTTCTAC ACTATGACAT CATTTGGCCT TCAGGTTTTG TGTGTGACAA CTGTTTGAAG AAAACTGGCA GACCTCGGAA AGAAAACAAA TTCAGTGCTA AGAGGCTGCA GACCACACGA TTGGGAAACC ACTTAGAAGA CAGAGTGAAT AAGTTTTTGC GGCGCCAGAA TCACCCTGAA GCTGGGGAGG TTTTTGTCAG AGTGGTGGCC AGCTCAGACA AGACTGTGGA GGTCAAGCCG GGAATGAAGT CAAGGTTTGT GGATTCTGGA GAGATGTCGG AATCTTTCCC ATATCGTACC AAAGCACTCT TTGCTTTTGA GGAGATCGAT GGAGTCGATG TGTGCTTTTT TGGGATGCAT GTGCAAGAAT ACGGCTCTGA TTGCCCCCCA CCAAATACAA GGCGTGTATA CATATCTTAT CTGGACAGTA TTCATTTCTT CCGGCCCCGC TGCCTCCGGA CAGCTGTTTA CCATGAGATC CTCATCGGAT ATCTCGAGTA TGTGAAGAAA TTGGGGTATG TGACAGGACA TATTTGGGCC TGTCCCCCAA GTGAAGGAGA TGACTATATC TTTCATTGCC ACCCCCCTGA CCAGAAAATC CCCAAACCAA AACGACTACA GGAGTGGTAC AAGAAGATGC TGGACAAGGC GTTTGCAGAG AGGATCATTA ACGACTATAA GGACATCTTC AAACAAGCGA ACGAAGACAG GCTCACGAGT GCCAAGGAGT TGCCCTATTT TGAAGGAGAT TTCTGGCCTA ATGTGTTGGA AGAAAGCATT AAGGAACTAG AACAAGAAGA AGAAGAAAGG AAAAAAGAAG AGAGTACTGC AGCGAGTGAG ACTCCTGAGG GCAGTCAGGG TGACAGCAAA AATGCGAAGA AAAAGAACAA CAAGAAGACC AACAAAAACA AAAGCAGCAT TAGCCGCGCC AACAAGAAGA AGCCCAGCAT GCCCAATGTT TCCAACGACC TGTCGCAGAA GCTGTATGCC ACCATGGAGA AGCACAAGGA GGTATTCTTT GTGATTCATC TGCATGCTGG GCCTGTTATC AGCACTCAGC CCCCCATCGT GGACCCTGAT CCTCTGCTTA GCTGTGACCT CATGGATGGG CGAGATGCCT TCCTCACCCT GGCCAGAGAC AAGCACTGGG AATTCTCTTC CTTACGCCGC TCCAAATGGT CCACTCTGTG CATGCTGGTG GAGCTGCACA CACAGGGCCA GGACCGCTTT GTTTATACCT GCAATGAGTG CAAACACCAT GTGGAAACAC GCTGGCACTG CACTGTGTGT GAGGACTATG ACCTTTGTAT CAATTGCTAC AACACAAAGA GCCACACCCA TAAGATGGTG AAGTGGGGGC TAGGCCTAGA TGATGAGGGC AGCAGTCAGG GTGAGCCACA GTCCAAGAGC CCCCAGGAAT CCCGGCGTCT CAGCATCCAG CGCTGCATCC AGTCCCTGGT GCATGCCTGC CAGTGTCGCA ATGCCAACTG CTCACTGCCG TCTTGCCAGA AGATGAAGCG AGTCGTGCAG CACACCAAGG GCTGCAAGCG CAAGACTAAT GGAGGATGCC CAGTGTGCAA GCAGCTCATT GCTCTTTGCT GCTACCACGC CAAACACTGC CAAGAAAATA AATGCCCTGT GCCCTTCTGC CTCAACATCA AACATAAGCT CCGCCAGCAG CAGATCCAGC ATCGCCTGCA GCAGGCTCAG CTCATGCGCC GGCGAATGGC AACCATGAAC ACCCGCAATG TGCCTCAGCA GAGTTTGCCT TCTCCTACCT CAGCACCACC CGGGACTCCT ACACAGCAGC CCAGCACACC CCAAACACCA CAGCCCCCAG CCCAGCCTCA GCCTTCACCT GTTAACATGT CACCAGCTGG CTTCCCTAAT GTAGCCCGGA CTCAGCCCCC AACAATAGTG TCTGCTGGGA AGCCTACCAA CCAGGTGCCA GCTCCCCCAC CCCCTGCCCA GCCCCCACCT GCAGCAGTAG AAGCAGCCCG GCAAATTGAA CGTGAGGCCC AGCAGCAGCA GCACCTATAC CGAGCAAACA TCAACAATGG CATGCCCCCA GGACGTGCAG GTATGGGGAC CCCAGGAAGC CAAATGACTC CTGTGGGCCT GAATGTGCCC CGTCCCAACC AAGTCAGTGG GCCTGTCATG TCTAGTATGC CACCTGGGCA GTGGCAGCAG GCACCCATCC CTCAGCAGCA GCCGATGCCA GGCATGCCCA GGCCTGTAAT GTCCATGCAG GCCCAGGCAG CAGTGGCTGG GCCACGGATG CCCAATGTGC AGCCACCAAG GAGCATCTCG CCAAGTGCCC TGCAAGACCT GCTACGGACC CTAAAGTCAC CCAGCTCTCC TCAGCAGCAG CAGCAGGTGC TGAACATCCT TAAATCAAAC CCACAGCTAA TGGCAGCTTT CATCAAACAG CGCACAGCCA AGTATGTGGC CAATCAGCCT GGCATGCAGC CCCAGCCCGG ACTTCAATCC CAGCCTGGTA TGCAGCCCCA GCCTGGCATG CACCAGCAGC CTAGTTTGCA AAACCTGAAC GCAATGCAAG CTGGTGTGCC ACGGCCTGGT GTGCCTCCAC CACAACCAGC AATGGGAGGC CTGAATCCCC AGGGACAAGC TCTGAACATC ATGAACCCAG GACACAACCC CAACATGACA AACATGAATC CACAGTACCG AGAAATGGTG AGGAGACAGC TGCTACAGCA CCAGCAGCAG CAGCAGCAAC AGCAGCAGCA GCAGCAGCAA CAACAAAATA GTGCCAGCTT GGCCGGGGGC ATGGCGGGAC ACAGCCAGTT CCAGCAGCCA CAAGGACCTG GAGGTTATGC CCCAGCCATG CAGCAGCAAC GCATGCAACA GCACCTCCCC ATCCAGGGCA GCTCCATGGG CCAGATGGCT GCTCCAATGG GACAACTTGG CCAGATGGGG CAGCCTGGGC TAGGGGCAGA CAGCACCCCT AATATCCAGC AGGCCCTGCA GCAACGGATT CTGCAGCAGC AGCAGATGAA GCAACAAATT GGGTCACCAG GCCAGCCGAA CCCCATGAGC CCCCAGCAGC ACATGCTCTC AGGACAGCCA CAGGCCTCAC ATCTCCCTGG CCAGCAGATC GCCACATCCC TTAGTAACCA GGTGCGATCT CCAGCCCCTG TGCAGTCTCC ACGGCCCCAA TCCCAACCTC CACATTCCAG CCCGTCACCA CGGATACAAC CCCAGCCTTC ACCACACCAT GTTTCACCCC AGACTGGTTC CCCTCACCCT GGACTCGCAG TCACCATGGC CAGCTCCATG GATCAGGGAC ACCTGGGGAA CCCTGAACAG AGTGCAATGC TCCCCCAGCT GAATACCCCC AACAGGAGCG CACTGTCCAG TGAACTGTCC CTGGTTGGTG ATACCACGGG AGACACACTA GAAAAGTTTG TGGAGGGTTT GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
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