pECMV-Irs2-m-FLAG
$84.00
In stock
SKU
PL85160
NM_001081212.2
100μg liquid plasmid(Instantly):$105
| name | pECMV-Irs2-m-FLAG | another name | apECMV-Irs2-m-FLAG |
| Insert Size (bp) | 4206bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Kan |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | pFLAG-CMV-R：GCACTGGAGTGGCAACTT | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGCTAGCGCG CCCCTGCCTG GGCCCCCCGC GTCGGCGGGC GGGGACGGCC CGAACCTCAA TAACAACAAC AACAACAACA ACCACAGCGT GCGCAAGTGC GGCTACCTGC GCAAGCAGAA GCACGGCCAC AAGCGCTTTT TCGTGTTGCG CGGCCCCGGC ACGGGCGGCG ACGAGGCATC CGCGGCTGGG GGGTCGCCGC CGCAGCCTCC GCGGCTGGAG TACTACGAGA GCGAGAAGAA GTGGAGGAGC AAGGCGGGCG CGCCGAAGCG AGTGATCGCG CTCGACTGCT GTCTGAACAT CAACAAGCGC GCGGACGCCA AGCACAAGTA CCTGATCGCC CTCTACACCA AGGACGAGTA CTTCGCTGTA GCGGCGGAGA ACGAGCAGGA GCAGGAGGGC TGGTACCGCG CACTCACCGA CTTGGTCAGC GAAGGCCGCT CTGGCGAGGG GGGCTCGGGC ACCACCGGAG GCTCTTGCAG CGCCTCTCTC CCGGGCGTCC TGGGCGGCTC AGCGGGCGCC GCTGGCTGCG ATGACAACTA CGGGCTCGTG ACACCCGCCA CGGCCGTCTA CCGCGAGGTG TGGCAGGTGA ACCTGAAACC TAAGGGACTG GGCCAGAGCA AGAACCTGAC TGGTGTATAC CGCCTATGCC TGTCTGCGCG CACCATCGGC TTCGTGAAGC TCAATTGCGA ACAGCCGTCG GTGACGCTGC AGCTTATGAA CATTCGCCGC TGCGGCCACT CGGACAGCTT CTTCTTCATC GAGGTGGGCC GTTCGGCCGT CACCGGTCCC GGGGAGCTGT GGATGCAAGC CGACGACTCG GTGGTGGCGC AGAACATCCA TGAGACCATC CTAGAAGCTA TGAAGGCACT CAAAGAGCTC TTCGAGTTCC GGCCTCGCAG CAAGAGTCAG TCGTCCGGGT CGTCAGCCAC GCATCCCATC AGCGTGCCGG GCGCGCGCCG CCACCACCAC CTAGTCAACC TACCCCCTAG CCAGACCGGC CTGGTGCGCC GCTCGCGCAC TGACAGCCTG GCGGCCACCC CCCCAGCAGC CAAGTGCACT TCGTGCCGGG TTCGTACGGC CAGCGAGGGC GACGGCGGCG CGGCAGGCGG GGCCGGGACG GCAGGAGGCA GGCCGATGTC GGTGGCAGGG AGCCCCCTGA GTCCCGGGCC GGTGCGCGCG CCCCTTAGCC GCTCGCACAC CCTGAGCGCC GGCTGCGGAG GCCGCCCGAG CAAAGTGACT CTGGCGCCGG CAGGGGGAGC CCTGCAACAC AGCCGCTCCA TGTCCATGCC CGTGGCGCAC TCACCTCCTG CAGCCACCAG CCCAGGCAGC CTGTCCTCCA GCAGTGGGCA CGGCTCGGGC TCCTACCCGC TGCCACCTGG CTCCCACCCG CACCTGCCTC ATCCACTGCA TCACCCCCAA GGCCAGCGTC CGTCCAGCGG TAGTGCCTCC GCCTCGGGCT CCCCCAGCGA CCCGGGTTTC ATGTCCCTTG ACGAGTATGG CTCCAGCCCT GGCGACCTGA GAGCCTTCAG TAGCCACAGG AGCAACACAC CCGAGTCAAT AGCGGAGACC CCGCCAGCCA GAGATGGCAG TGGGGGCGAA CTCTATGGGT ACATGAGCAT GGATAGACCC CTGAGCCACT GTGGCCGCCC TTACCGTAGG GTCTCAGGGG ATGGGGCCCA GGACCTGGAT AGAGGACTGA GGAAGAGGAC TTATTCCCTA ACCACGCCTG CCAGGCAGCG GCAGGTACCT CAGCCTTCCT CTGCCTCTCT AGATGAATAC ACTCTCATGA GGGCCACCTT CTCTGGTAGT TCAGGTCGCC TCTGCCCATC CTTCCCTGCG TCCTCTCCCA AAGTGGCCTA CAACCCTTAC CCAGAGGACT ATGGAGACAT TGAGATTGGT TCTCACAAGA GTTCCAGCAG TAACCTGGGG GCAGATGATG GCTACATGCC CATGACCCCT GGGGCAGCCC TTAGGAGTGG TGGTCCCAAT AGCTGCAAGA GCGATGACTA CATGCCCATG AGCCCCACAA GCGTGTCTGC TCCCAAGCAG ATCCTGCAGC CACGCTTGGC AGCGGCCTTG CCCCCTTCCG GAGCAGCCGT GCCAGCACCC CCTTCAGGGG TGGGCAGGAC CTTCCCAGTA AACGGAGGTG GCTACAAAGC CAGCTCCCCA GCGGAGAGCT CCCCAGAAGA CAGTGGGTAC ATGCGAATGT GGTGTGGCTC CAAGCTGTCT ATGGAGAACC CAGACCCTAA GCTACTCCCC AACGGGGACT ACCTCAACAT GTCCCCCAGC GAGGCAGGCA CTGCAGGGAC CCCACCTGAC TTCTCAGCAG CTTTGCGTGG AGGCAGTGAA GGCCTCAAAG GCATCCCGGG CCACTGCTAC AGCTCTTTGC CCCGCTCTTA TAAGGCTCCC TGTTCCTGCA GCGGAGACAA TGACCAGTAT GTGCTCATGA GCTCCCCTGT GGGCCGGATC TTGGAAGAGG AGAGACTGGA GCCCCAGGCC ACCCCAGGGG CTGGCACCTT TGGGGCAGCT GGTGGTAGTC ATACCCAGCC TCATCACTCA GCAGTGCCTT CCTCCATGAG GCCGAGTGCC ATCGGTGGCC GCCCTGAGGG CTTCCTGGGC CAGCGATGTC GGGCAGTGCG GCCTACACGC CTATCGCTAG AGGGACTGCA GACCCTTCCC AGCATGCAAG AGTACCCTCT ACCCACAGAG CCCAAGAGCC CTGGCGAGTA CATCAACATT GACTTTGGTG AGGCAGGTAC CCGTCTGTCT CCGCCTGCCC CCCCACTACT GGCATCCGCG GCCTCATCTT CTTCACTGCT CTCAGCTAGT AGTCCTGCTT CATCCCTGGG TTCAGGAACC CCAGGCACCA GCAGCGACAG CCGGCAGCGC TCTCCACTCT CTGACTATAT GAACCTGGAC TTCAGTTCTC CCAAGTCCCC CAAGCCTAGC ACCCGCAGTG GGGACACAGT AGGCTCCATG GATGGCCTTC TCTCTCCAGA GGCTTCATCC CCATACCCAC CACTGCCCCC ACGTCCTTCC ACTTCCCCTT CCTCCTTACA GCAGCCTCTG CCACCTGCCC CGGGAGACCT ATACCGCCTG CCTCCAGCAT CAGCTGCCAC TTCCCAGGGT CCCACTGCTG GCTCCTCAAT GTCCTCCGAG CCTGGGGATA ATGGTGACTA TACCGAGATG GCCTTTGGTG TGGCTGCAAC CCCGCCACAA CCTATCGTGG CACCTCCAAA GCCAGAAGGT GCCCGAGTGG CCAGTCCCAC ATCGGGCTTG AAGCGGCTAA GTCTCATGGA TCAGGTATCT GGGGTGGAGG CTTTCCTTCA AGTCAGCCAG CCCCCTGACC CCCACCGGGG TGCTAAGGTC ATCCGTGCAG ACCCACAGGG GGGACGTCGT CGCCACAGTT CAGAGACCTT TTCCTCTACC ACCACCGTCA CCCCAGTGTC CCCATCCTTT GCCCACAATT CCAAGCGCCA CAATTCGGCC TCTGTGGAAA ATGTCTCACT CAGGAAAAGC AGTGAAGGCA GCAGTACCCT GGGAGGAGGT GATGAGCCGC CCACATCCCC AGGACAGGCA CAGCCCTTGG TGGCTGTGCC CCCAGTGCCA CAGGCTAGGC CGTGGAACCC CGGTCAGCCC GGAGCTTTGA TTGGCTGTCC TGGAGGCAGC AGTTCTCCCA TGCGCAGAGA GACCTCCGTG GGTTTCCAGA ACGGCCTCAA CTATATCGCC ATCGATGTGA GAGGCGAGCA GGGGTCCTTG GCGCAGTCTC AGCCGCAGCC AGGAGACAAG AACTCCTGGA GCCGGACCCG TAGCCTTGGG GGGCTCCTCG GCACCGTCGG AGGCTCTGGC GCCAGCGGAG TGTGTGGGGG TCCAGGCACT GGAGCTTTGC CCTCTGCCAG CACCTATGCA AGCATCGACT TCCTGTCCCA TCACTTGAAG GAAGCCACAG TCGTGAAAGA GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
