NM_033218.1;AI608257;bHLHd2;lop13;nuc;SREBP-2;SREBP2;SREBP2gc
100μg liquid plasmid(Instantly):$134
| name | pECMV-Srebf2-m-FLAG | another name | apECMV-Srebf2-m-FLAG |
| Insert Size (bp) | 3393bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Kan |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | pFLAG-CMV-R：GCACTGGAGTGGCAACTT | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGATGAGAGC AGCGAGCTGG GCGTTCTGGA GACCATGGAG ACCCTCACGG AGCTGGGCGA TGAGCTGACT CTCGGGGACA TCGACGAGAT GCTACAGTTT GTCAGCAATC AAGTGGGAGA GTTCCCTGAT TTGTTTTCAG AGCAACTTTG TAGCTCCTTC CCTGGAGGTG GCAGCAATGG TGGCAGTGGC AACAACAGCA GTGGCAGAGG CAACAATGGT GGAGCCACAG ACCCTGCAGT ACAGCGGTCA TTCAGCCAGG TCCCATTATC TACCTTCTCT CCCTCAGCTG CCTCCCCTCA GGCTCCGGCC CTGCAGGTCA AAGTCTCTCC TACCCCACCA AGGGCAACCC CTGTTCTCCA GCCGCGACCC CAGCCCCAGC CCCAACCTCC AGCTCAGCTG CAGCAGCAGA CGGTGATGAT CACCCCGACG TTCAGCACCG CTCCGCAGAC GAGGATCATC CAGCAGCCTT TGATATACCA GAACGCAGCG ACCAGCTTTC AAGTCCTGCA GCCTCAAGTG CAAAGCCTCG TGACGTCCCC GCAGGTGCAG CCCGTCACCA TCCAGCAGCA GGTGCAGACG GTACAGGCCC AGCGGGTGTT GACGCAGACA GCCAATGGCA CACTGCAGAC CCTTGCCCCC GCCACGGTAC AGACGGTCGC TGCGCCACAG GTGCAGCAGG TCCCGGTCCT GGTGCAACCT CAGATCATTA AGACAGATTC CCTTGTGTTG ACCACGCTGA AGACAGATGG CAGCCCTGTC ATGGCTGCAG TCCAGAACCC GGCCCTCACG GCCCTCACTG CCCCCATCCA GACAGCCGCC CTTCAAGTGC CAACCCTGGT GGGCAGCAAC GGGACCATTC TGACCACAAT GCCTGTGATG ATGGGGCAAG AGAAAGTTCC TATCAAGCAA GTGCCTGGCG GCGTGAAGCA GCTGGATCCT CCCAAAGAAG GAGAGAGGCG GACAACACAC AATATCATTG AAAAGCGCTA CCGGTCCTCC ATCAACGACA AAATCATAGA GTTGAAGGAC TTAGTCATGG GGACAGATGC CAAGATGCAC AAGTCTGGCG TTCTGAGGAA GGCCATTGAT TACATCAAAT ATCTGCAGCA GGTCAATCAC AAGCTGCGCC AGGAGAACAT GGTGCTGAAG CTGGCCAATC AGAAAAACAA GCTCCTGAAG GGCATCGACC TGGGCAGTCT GGTGGACAGT GATGTGGACT TGAAAATTGA TGACTTTAAC CAGAATGTCC TTCTGATGTC TCCGCCGGCC TCCGACTCCG GGTCCCAGGC CGGCTTCTCT CCCTATTCCA TTGACTCTGA GCCGGGCAGC CCTCTGCTGG ATGACGCAAA GGTCAAGGAT GAACCGGACT CTCCTCCTGT GGCACTGGGC ATGGTGGACC GCTCTCGAAT CCTCTTATGT GTCCTCACCT TCCTGGGCCT CTCCTTTAAC CCCTTGACTT CCTTGCTGCA GTGGGGAGGG GCCCACAACA CTGACCAGCA CCCATACTCA GGCTCGGGCC GCAGTGTACT GTCACTGGAG TCAGGTGCTG GGGGCTGGTT TGACTGGATG GTTCCAACTC TCCTCCTGTG GCTGGTAAAT GGTGTGATTG TCTTGAGCGT CTTTGTGAAG CTGTTGGTCC ACGGGGAGCC GGTGATTCGC CCACACTCAC GCCCCTCAGT CACCTTCTGG AGACACCGGA AGCAGGCAGA CCTAGACCTC GCCAAAGGTG ATTTCGCAGC TGCTGCTGCC AACCTACAAA CCTGCTTATC CGTGCTGGGC CGGGCGCTGC CCACCTCCCG CCTGGACTTG GCCTGCAGCC TCTCCTGGAA TGTGATCCGC TACAGCCTGC AGAAGCTGCG CCTGGTACGC TGGTTACTCA AGAAGGTCTT CCAGCGCTGG CGGGCTACTC CCGCCACTGC AGCTGGCTTT GAGGATGAGG CTAAGAGCAG CGCAAGGGAT GCGGCACTGG CCTACCACAG GCTGCACCAG CTGCACATCA CAGGGAAGCT TCCGGCGGGA TCTGCCTGTT CAGATGTGCA CATGGCTCTG TGTGCTGTGA ACCTGGCCGA GTGTGCGGAG GAGAAAATCC TACCAAGCAC ACTGATTGAG ATCCACCTGA CAGCGGCCAT GGGGCTCAAG ACCCGCTGTG GAGGCAAGCT GGGCTTCTTG GCTAGCTACT TCTTAAATCG TGCCCAGAGT CTGTGTGGCC CAGAGCATAG CACTGTCCCC GACTCCCTGC GCTGGCTATG CCATCCCTTG GGCCAGAAGT TTTTCATGGA GCGAAGCTGG TCGATCAAGT CAGCAGCCAA GGAGAGCCTG TACTGCGCCC AGAGGAGCCC AGCTGACCCC ATTGCCCAGG TACACCAGGC GTTCTGCAAA AACCTGCTAG AAAGAGCGGT GGAGTCCTTG GTGAAACCTC AGGCCAAGAA GAAGGCAGGC GACCAGGAAG AAGAGAGCTG TGAATTTTCC AGTGCTCTAG AGTATTTGAA ATTACTTCAT TCTTTTGTGG ACTCTGTGGG ATTTGTGACC TCACCATTCT CCAGCAGTTC CGTGCTCAGG TCGGCCCTTG GCCCCGATGT CATCTGCCGG TGGTGGACGT CCGCAGTCAC CATGGCCATC AGCTGGCTCC AGGGAGACGA TGCAGCTGTG CGCTCTCGTT TTACTGAAGT AGAGCGGGTC CCCAAGGCCC TGGAAGTGAC CGAGAGTCCC TTGGTGAAGG CCGTCTTCTA TACCTGTAGA GCCATGCATG CCTCCCTCTC TGGGAAAGCT GACGGGCAGC AGAACTCCTT CTGTCACTGT GAGAGGGCCA GTGGTCACCT GTGGAGCAGT CTCAACGTCA GCGGCACTAC TTCTGATCCT TCACTTAACC ATGTGATCCA GCTGTTCACC TGTGACCTGC TACTGTCGCT GAGGACAGCA CTCTGGCAAA AGCAGGCCAG TGCCAGCCAG CTCCTGGGTG AGACCTACCA TGCATCCGGC ACTGAGCTGG CCGGCTTCCA GCGGGACCTG GGCAGCCTTC GAAGGCTGGC CCATAGCTTC CGCCCAGCCT ACCGCAAGGT GTTCCTGCAT GAAGCCACTG TGCGCCTGAT GGCAGGAGCC AGCCCTACCC GTACACACCA GCTGCTGGAG CATAGCCTAC GGCGGCGCCC CACTCAGAAC ACCAAGCATG GAGAGGTAGA CACCTGGCCT GGCCAACGAG AGCGAGCTAC TGCCATCCTG CTGGCCTGCC GCCATCTTCC CCTCTCTTTC CTGTCTTCCC CGGGCCAGCG GGCAGTGCTG CTGGCTGAAG CTGCCCGCAC CCTGGAGAAG GTGGGTGACC GCCGTTCCTG CAGTGACTGC CAGCAGATGA TTGTCAAGCT GGGCGGTGGC ACTGCCATCG CTGCCTCCAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
