pSPYCE-35S
$128.00
In stock
SKU
PL85086
100μg liquid plasmid(Instantly):$149
| name | pSPYCE-35S | another name | apSPYCE-35S |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Bimolecular fluorescence | Fragment type | BIFC |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | 35s | Replicon | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGAGCGTCGC AAAGGCGCTC GGTCTTGCCT TGCTCGTCGG TGATGTACTT CACCAGCTCC GCGAAGTCGC TCTTCTTGAT GGAGCGCATG GGGACGTGCT TGGCAATCAC GCGCACCCCC CGGCCGTTTT AGCGGCTAAA AAAGTCATGG CTCTGCCCTC GGGCGGACCA CGCCCATCAT GACCTTGCCA AGCTCGTCCT GCTTCTCTTC GATCTTCGCC AGCAGGGCGA GGATCGTGGC ATCACCGAAC CGCGCCGTGC GCGGGTCGTC GGTGAGCCAG AGTTTCAGCA GGCCGCCCAG GCGGCCCAGG TCGCCATTGA TGCGGGCCAG CTCGCGGACG TGCTCATAGT CCACGACGCC CGTGATTTTG TAGCCCTGGC CGACGGCCAG CAGGTAGGCC GACAGGCTCA TGCCGGCCGC CGCCGCCTTT TCCTCAATCG CTCTTCGTTC GTCTGGAAGG CAGTACACCT TGATAGGTGG GCTGCCCTTC CTGGTTGGCT TGGTTTCATC AGCCATCCGC TTGCCCTCAT CTGTTACGCC GGCGGTAGCC GGCCAGCCTC GCAGAGCAGG ATTCCCGTTG AGCACCGCCA GGTGCGAATA AGGGACAGTG AAGAAGGAAC ACCCGCTCGC GGGTGGGCCT ACTTCACCTA TCCTGCCCGG CTGACGCCGT TGGATACACC AAGGAAAGTC TACACGAACC CTTTGGCAAA ATCCTGTATA TCGTGCGAAA AAGGATGGAT ATACCGAAAA AATCGCTATA ATGACCCCGA AGCAGGGTTA TGCAGCGGAA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCAGA AGGCCGCCAG AGAGGCCGAG CGCGGCCGTG AGGCTTGGAC GCTAGGGCAG GGCATGAAAA AGCCCGTAGC GGGCTGCTAC GGGCGTCTGA CGCGGTGGAA AGGGGGAGGG GATGTTGTCT ACATGGCTCT GCTGTAGTGA GTGGGTTGCG CTCCGGCAGC GGTCCTGATC AATCGTCACC CTTTCTCGGT CCTTCAACGT TCCTGACAAC GAGCCTCCTT TTCGCCAATC CATCGACAAT CACCGCGAGT CCCTGCTCGA ACGCTGCGTC CGGACCGGCT TCGTCGAAGG CGTCTATCGC GGCCCGCAAC AGCGGCGAGA GCGGAGCCTG TTCAACGGTG CCGCCGCGCT CGCCGGCATC GCTGTCGCCG GCCTGCTCCT CAAGCACGGC CCCAACAGTG AAGTAGCTGA TTGTCATCAG CGCATTGACG GCGTCCCCGG CCGAAAAACC CGCCTCGCAG AGGAAGCGAA GCTGCGCGTC GGCCGTTTCC ATCTGCGGTG CGCCCGGTCG CGTGCCGGCA TGGATGCGCG CGCCATCGCG GTAGGCGAGC AGCGCCTGCC TGAAGCTGCG GGCATTCCCG ATCAGAAATG AGCGCCAGTC GTCGTCGGCT CTCGGCACCG AATGCGTATG ATTCTCCGCC AGCATGGCTT CGGCCAGTGC GTCGAGCAGC GCCCGCTTGT TCCTGAAGTG CCAGTAAAGC GCCGGCTGCT GAACCCCCAA CCGTTCCGCC AGTTTGCGTG TCGTCAGACC GTCTACGCCG ACCTCGTTCA ACAGGTCCAG GGCGGCACGG ATCACTGTAT TCGGCTGCAA CTTTGTCATG CTTGACACTT TATCACTGAT AAACATAATA TGTCCACCAA CTTATCAGTG ATAAAGAATC CGCGCGTTCA ATCGGACCAG CGGAGGCTGG TCCGGAGGCC AGACGTGAAA CCCAACATAC CCCTGATCGT AATTCTGAGC ACTGTCGCGC TCGACGCTGT CGGCATCGGC CTGATTATGC CGGTGCTGCC GGGCCTCCTG CGCGATCTGG TTCACTCGAA CGACGTCACC GCCCACTATG GCATTCTGCT GGCGCTGTAT GCGTTGGTGC AATTTGCCTG CGCACCTGTG CTGGGCGCGC TGTCGGATCG TTTCGGGCGG CGGCCAATCT TGCTCGTCTC GCTGGCCGGC GCCAGATCTG GGGAACCCTG TGGTTGGCAT GCACATACAA ATGGACGAAC GGATAAACCT TTTCACGCCC TTTTAAATAT CCGATTATTC TAATAAACGC TCTTTTCTCT TAGGTTTACC CGCCAATATA TCCTGTCAAA CACTGATAGT TTAAACTGAA GGCGGGAAAC GACAATCTGA TCATGAGCGG AGAATTAAGG GAGTCACGTT ATGACCCCCG CCGATGACGC GGGACAAGCC GTTTTACGTT TGGAACTGAC AGAACCGCAA CGTTGAAGGA GCCACTCAGC CGCGGGTTTC TGGAGTTTAA TGAGCTAAGC ACATACGTCA GAAACCATTA TTGCGCGTTC AAAAGTCGCC TAAGGTCACT ATCAGCTAGC AAATATTTCT TGTCAAAAAT GCTCCACTGA CGTTCCATAA ATTCCCCTCG GTATCCAATT AGAGTCTCAT ATTCACTCTC AATCCAAATA ATCTGCACCG GATCTGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GATGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACGGGATCTC TGCGGAACAG GCGGTCGAAG GTGCCGATAT CATTACGACA GCAACGGCCG ACAAGCACAA CGCCACGATC CTGAGCGACA ATATGATCGG GCCCGGCGTC CACATCAACG GCGTCGGCGG CGACTGCCCA GGCAAGACCG AGATGCACCG CGATATCTTG CTGCGTTCGG ATATTTTCGT GGAGTTCCCG CCACAGACCC GGATGATCCC CGATCGTTCA AACATTTGGC AATAAAGTTT CTTAAGATTG AATCCTGTTG CCGGTCTTGC GATGATTATC ATATAATTTC TGTTGAATTA CGTTAAGCAT GTAATAATTA ACATGTAATG CATGACGTTA TTTATGAGAT GGGTTTTTAT GATTAGAGTC CCGCAATTAT ACATTTAATA CGCGATAGAA AACAAAATAT AGCGCGCAAA CTAGGATAAA TTATCGCGCG CGGTGTCATC TATGTTACTA GATCGGGCCT CCTGTCAATG CTGGCGGCGG CTCTGGTGGT GGTTCTGGTG GCGGCTCTGA GGGTGGTGGC TCTGAGGGTG GCGGTTCTGA GGGTGGCGGC TCTGAGGGAG GCGGTTCCGG TGGTGGCTCT GGTTCCGGTG ATTTTGATTA TGAAAAGATG GCAAACGCTA ATAAGGGGGC TATGACCGAA AATGCCGATG AAAACGCGCT ACAGTCTGAC GCTAAAGGCA AACTTGATTC TGTCGCTACT GATTACGGTG CTGCTATCGA TGGTTTCATT GGTGACGTTT CCGGCCTTGC TAATGGTAAT GGTGCTACTG GTGATTTTGC TGGCTCTAAT TCCCAAATGG CTCAAGTCGG TGACGGTGAT AATTCACCTT TAATGAATAA TTTCCGTCAA TATTTACCTT CCCTCCCTCA ATCGGTTGAA TGTCGCCCTT TTGTCTTTGG CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGCCA AGCTTGCATG CCTGCAGGTC CCCAGATTAG CCTTTTCAAT TTCAGAAAGA ATGCTAACCC ACAGATGGTT AGAGAGGCTT ACGCAGCAGG TCTCATCAAG ACGATCTACC CGAGCAATAA TCTCCAGGAA ATCAAATACC TTCCCAAGAA GGTTAAAGAT GCAGTCAAAA GATTCAGGAC TAACTGCATC AAGAACACAG AGAAAGATAT ATTTCTCAAG ATCAGAAGTA CTATTCCAGT ATGGACGATT CAAGGCTTGC TTCACAAACC AAGGCAAGTA ATAGAGATTG GAGTCTCTAA AAAGGTAGTT CCCACTGAAT CAAAGGCCAT GGAGTCAAAG ATTCAAATAG AGGACCTAAC AGAACTCGCC GTAAAGACTG GCGAACAGTT CATACAGAGT CTCTTACGAC TCAATGACAA GAAGAAAATC TTCGTCAACA TGGTGGAGCA CGACACACTT GTCTACTCCA AAAATATCAA AGATACAGTC TCAGAAGACC AAAGGGCAAT TGAGACTTTT CAACAAAGGG TAATATCCGG AAACCTCCTC GGATTCCATT GCCCAGCTAT CTGTCACTTT ATTGTGAAGA TAGTGGAAAA GGAAGGTGGC TCCTACAAAT GCCATCATTG CGATAAAGGA AAGGCCATCG TTGAAGATGC CTCTGCCGAC AGTGGTCCCA AAGATGGACC CCCACCCACG AGGAGCATCG TGGAAAAAGA AGACGTTCCA ACCACGTCTT CAAAGCAAGT GGATTGATGT GATATCTCCA CTGACGTAAG GGATGACGCA CAATCCCACT ATCCTTCGCA AGACCCTTCC TCTATATAAG GAAGTTCATT TCATTTGGAG AGAACACGGG GGACTCTAGA GTTAACCGGG CTCAGGCCTG GCGCGCCACT AGTGGATCCA TCGATAGTAC TGTCGACCTC GAGGGTACCG CTCCCGGGAT GTACCCATAC GATGTTCCAG ATTACGCTGA CAAGCAGAAG AACGGCATCA AGGTGAACTT CAAGATCCGC CACAACATCG AGGACGGCAG CGTGCAGCTC GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA GCTACCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGACGA GCTGTACAAG TAAGAGCTCG AATTTCCCCG ATCGTTCAAA CATTTGGCAA TAAAGTTTCT TAAGATTGAA TCCTGTTGCC GGTCTTGCGA TGATTATCAT ATAATTTCTG TTGAATTACG TTAAGCATGT AATAATTAAC ATGTAATGCA TGACGTTATT TATGAGATGG GTTTTTATGA TTAGAGTCCC GCAATTATAC ATTTAATACG CGATAGAAAA CAAAATATAG CGCGCAAACT AGGATAAATT ATCGCGCGCG GTGTCATCTA TGTTACTAGA TCGGGAATTC ACTGGCCGTC GTTTTACAAC GTCGTGACTG GGAAAACCCT GGCGTTACCC AACTTAATCG CCTTGCAGCA CATCCCCCTT TCGCCAGCTG GCGTAATAGC GAAGAGGCCC GCACCGATCG CCCTTCCCAA CAGTTGCGCA GCCTGAATGG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTTGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG GGCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG AACCACCATC AAACAGGATT TTCGCCTGCT GGGGCAAACC AGCGTGGACC GCTTGCTGCA ACTCTCTCAG GGCCAGGCGG TGAAGGGCAA TCAGCTGTTG CCCGTCTCAC TGGTGAAAAG AAAAACCACC CCAGTACATT AAAAACGTCC GCAATGTGTT ATTAAGTTGT CTAAGCGTCA ATTTGTTTAC ACCACAATAT ATCCTGCCAC CAGCCAGCCA ACAGCTCCCC GACCGGCAGC TCGGCACAAA ATCACCACTC GATACAGGCA GCCCATCAGT CCGGGACGGC GTCAGCGGGA GAGCCGTTGT AAGGCGGCAG ACTTTGCTCA TGTTACCGAT GCTATTCGGA AGAACGGCAA CTAAGCTGCC GGGTTTGAAA CACGGATGAT CTCGCGGAGG GTAGCATGTT GATTGTAACG ATGACAGAGC GTTGCTGCCT GTGATCAAAT ATCATCTCCC TCGCAGAGAT CCGAATTATC AGCCTTCTTA TTCATTTCTC GCTTAACCGT GACAGGCTGT CGATCTTGAG AACTATGCCG ACATAATAGG AAATCGCTGG ATAAAGCCGC TGAGGAAGCT GAGTGGCGCT ATTTCTTTAG AAGTGAACGT TGACGATATC AACTCCCCTA TCCATTGCTC ACCGAATGGT ACAGGTCGGG GACCCGAAGT TCCGACTGTC GGCCTGATGC ATCCCCGGCT GATCGACCCC AGATCTGGGG CTGAGAAAGC CCAGTAAGGA AACAACTGTA GGTTCGAGTC GCGAGATCCC CCGGAACCAA AGGAAGTAGG TTAAACCCGC TCCGATCAGG CCGAGCCACG CCAGGCCGAG AACATTGGTT CCTGTAGGCA TCGGGATTGG CGGATCAAAC ACTAAAGCTA CTGGAACGAG CAGAAGTCCT CCGGCCGCCA GTTGCCAGGC GGTAAAGGTG AGCAGAGGCA CGGGAGGTTG CCACTTGCGG GTCAGCACGG TTCCGAACGC CATGGAAACC GCCCCCGCCA GGCCCGCTGC GACGCCGACA GGATCTAGCG CTGCGTTTGG TGTCAACACC AACAGCGCCA CGCCCGCAGT TCCGCAAATA GCCCCCAGGA CCGCCATCAA TCGTATCGGG CTACCTAGCA GAGCGGCAGA GATGAACACG ACCATCAGCG GCTGCACAGC GCCTACCGTC GCCGCGACCC CGCCCGGCAG GCGGTAGACC GAAATAAACA ACAAGCTCCA GAATAGCGAA ATATTAAGTG CGCCGAGGAT GAAGATGCGC ATCCACCAGA TTCCCGTTGG AATCTGTCGG ACGATCATCA CGAGCAATAA ACCCGCCGGC AACGCCCGCA GCAGCATACC GGCGACCCCT CGGCCTCGCT GTTCGGGCTC CACGAAAACG CCGGACAGAT GCGCCTTGTG AGCGTCCTTG GGGCCGTCCT CCTGTTTGAA GACCGACAGC CCAATGATCT CGCCGTCGAT GTAGGCGCCG AATGCCACGG CATCTCGCAA CCGTTCAGCG AACGCCTCCA TGGGCTTTTT CTCCTCGTGC TCGTAAACGG ACCCGAACAT CTCTGGAGCT TTCTTCAGGG CCGACAATCG GATCTCGCGG AAATCCTGCA CGTCGGCCGC TCCAAGCCGT CGAATCTGAG CCTTAATCAC AATTGTCAAT TTTAATCCTC TGTTTATCGG CAGTTCGTAG AGCGCGCCGT GCGTCCCGAG CGATACTGAG CGAAGCAAGT GCGTCGAGCA GTGCCCGCTT GTTCCTGAAA TGCCAGTAAA GCGCTGGCTG CTGAACCCCC AGCCGGAACT GACCCCACAA GGCCCTAGCG TTTGCAATGC ACCAGGTCAT CATTGACCCA GGCGTGTTCC ACCAGGCCGC TGCCTCGCAA CTCTTCGCAG GCTTCGCCGA CCTGCTCGCG CCACTTCTTC ACGCGGGTGG AATCCGATCC GCACATGAGG CGGAAGGTTT CCAGCTTGAG CGGGTACGGC TCCCGGTGCG AGCTGAAATA GTCGAACATC CGTCGGGCCG TCGGCGACAG CTTGCGGTAC TTCTCCCATA TGAATTTCGT GTAGTGGTCG CCAGCAAACA GCACGACGAT TTCCTCGTCG ATCAGGACCT GGCAACGGGA CGTTTTCTTG CCACGGTCCA GGACGCGGAA GCGGTGCAGC AGCGACACCG ATTCCAGGTG CCCAACGCGG TCGGACGTGA AGCCCATCGC CGTCGCCTGT AGGCGCGACA GGCATTCCTC GGCCTTCGTG TAATACCGGC CATTGATCGA CCAGCCCAGG TCCTGGCAAA GCTCGTAGAA CGTGAAGGTG ATCGGCTCGC CGATAGGGGT GCGCTTCGCG TACTCCAACA CCTGCTGCCA CACCAGTTCG TCATCGTCGG CCCGCAGCTC GACGCCGGTG TAGGTGATCT TCACGTCCTT GTTGACGTGG AAAATGACCT TGTTTTGCAG CGCCTCGCGC GGGATTTTCT TGTTGCGCGT GGTGAACAGG GCAGAGCGGG CCGTGTCGTT TGGCATCGCT CGCATCGTGT CCGGCCACGG CGCAATATCG AACAAGGAAA GCTGCATTTC CTTGATCTGC TGCTTCGTGT GTTTCAGCAA CGCGGCCTGC TTGGCCTCGC TGACCTGTTT TGCCAGGTCC TCGCCGGCGG TTTTTCGCTT CTTGGTCGTC ATAGTTCCTC GCGTGTCGAT GGTCATCGAC TTCGCCAAAC CTGCCGCCTC CTGTTCGAGA CGACGCGAAC GCTCCACGGC GGCCGATGGC GCGGGCAGGG CAGGGGGAGC CAGTTGCACG CTGTCGCGCT CGATCTTGGC CGTAGCTTGC TGGACCATCG AGCCGACGGA CTGGAAGGTT TCGCGGGGCG CACGCATGAC GGTGCGGCTT GCGATGGTTT CGGCATCCTC GGCGGAAAAC CCCGCGTCGA TCAGTTCTTG CCTGTATGCC TTCCGGTCAA ACGTCCGATT CATTCACCCT CCTTGCGGGA TTGCCCCGAC TCACGCCGGG GCAATGTGCC CTTATTCCTG ATTTGACCCG CCTGGTGCCT TGGTGTCCAG ATAATCCACC TTATCGGCAA TGAAGTCGGT CCCGTAGACC GTCTGGCCGT CCTTCTCGTA CTTGGTATTC CGAATCTTGC CCTGCACGAA TACCAGCGAC CCCTTGCCCA AATACTTGCC GTGGGCCTCG GCCTGAGAGC CAAAACACTT GATGCGGAAG AAGTCGGTGC GCTCCTGCTT GTCGCCGGCA TCGTTGCGCC ACATCTAGGT ACTAAAACAA TTCATCCAGT AAAATATAAT ATTTTATTTT CTCCCAATCA GGCTTGATCC CCAGTAAGTC AAAAAATAGC TCGACATACT GTTCTTCCCC GATATCCTCC CTGATCGACC GGACGCAGAA GGCAATGTCA TACCACTTGT CCGCCCTGCC GCTTCTCCCA AGATCAATAA AGCCACTTAC TTTGCCATCT TTCACAAAGA TGTTGCTGTC TCCCAGGTCG CCGTGGGAAA AGACAAGTTC CTCTTCGGGC TTTTCCGTCT TTAAAAAATC ATACAGCTCG CGCGGATCTT TAAATGGAGT GTCTTCTTCC CAGTTTTCGC AATCCACATC GGCCAGATCG TTATTCAGTA AGTAATCCAA TTCGGCTAAG CGGCTGTCTA AGCTATTCGT ATAGGGACAA TCCGATATGT CGATGGAGTG AAAGAGCCTG ATGCACTCCG CATACAGCTC GATAATCTTT TCAGGGCTTT GTTCATCTTC ATACTCTTCC GAGCAAAGGA CGCCATCGGC CTCACTCATG AGCAGATTGC TCCAGCCATC ATGCCGTTCA AAGTGCAGGA CCTTTGGAAC AGGCAGCTTT CCTTCCAGCC ATAGCATCAT GTCCTTTTCC CGTTCCACAT CATAGGTGGT CCCTTTATAC CGGCTGTCCG TCATTTTTAA ATATAGGTTT TCATTTTCTC CCACCAGCTT ATATACCTTA GCAGGAGACA TTCCTTCCGT ATCTTTTACG CAGCGGTATT TTTCGATCAG TTTTTTCAAT TCCGGTGATA TTCTCATTTT AGCCATTTAT TATTTCCTTC CTCTTTTCTA CAGTATTTAA AGATACCCCA AGAAGCTAAT TATAACAAGA CGAACTCCAA TTCACTGTTC CTTGCATTCT AAAACCTTAA ATACCAGAAA ACAGCTTTTT CAAAGTTGTT TTCAAAGTTG GCGTATAACA TAGTATCGAC GGAGCCGATT TTGAAACCAC AATTATGGGT GATGCTGCCA ACTTACTGAT TTAGTGTATG ATGGTGTTTT TGAGGTGCTC CAGTGGCTTC TGTGTCTATC AGCTGTCCCT CCTGTTCAGC TACTGACGGG GTGGTGCGTA ACGGCAAAAG CACCGCCGGA CATCAGCGCT ATCTCTGCTC TCACTGCCGT AAAACATGGC AACTGCAGTT CACTTACACC GCTTCTCAAC CCGGTACGCA CCAGAAAATC ATTGATATGG CCATGAATGG CGTTGGATGC CGGGCAACAG CCCGCATTAT GGGCGTTGGC CTCAACACGA TTTTACGTCA CTTAAAAAAC TCAGGCCGCA GTCGGTAACC TCGCGCATAC AGCCGGGCAG TGACGTCATC GTCTGCGCGG AAATGGACGA ACAGTGGGGC TATGTCGGGG CTAAATCGCG CCAGCGCTGG CTGTTTTACG CGTATGACAG TCTCCGGAAG ACGGTTGTTG CGCACGTATT CGGTGAACGC ACTATGGCGA CGCTGGGGCG TCTTATGAGC CTGCTGTCAC CCTTTGACGT GGTGATATGG ATGACGGATG GCTGGCCGCT GTATGAATCC CGCCTGAAGG GAAAGCTGCA CGTAATCAGC AAGCGATATA CGCAGCGAAT TGAGCGGCAT AACCTGAATC TGAGGCAGCA CCTGGCACGG CTGGGACGGA AGTCGCTGTC GTTCTCAAAA TCGGTGGAGC TGCATGACAA AGTCATCGGG CATTATCTGA ACATAAAACA CTATCAATAA GTTGGAGTCA TTACCCAATT ATGATAGAAT TTACAAGCTA TAAGGTTATT GTCCTGGGTT TCAAGCATTA GTCCATGCAA GTTTTTATGC TTTGCCCATT CTATAGATAT ATTGATAAGC GCGCTGCCTA TGCCTTGCCC CCTGAAATCC TTACATACGG CGATATCTTC TATATAAAAG ATATATTATC TTATCAGTAT TGTCAATATA TTCAAGGCAA TCTGCCTCCT CATCCTCTTC ATCCTCTTCG TCTTGGTAGC TTTTTAAATA TGGCGCTTCA TAGAGTAATT CTGTAAAGGT CCAATTCTCG TTTTCATACC TCGGTATAAT CTTACCTATC ACCTCAAATG GTTCGCTGGG TTTATCGCAC CCCCGAACAC GAGCACGGCA CCCGCGACCA CTATGCCAAG AATGCCCAAG GTAAAAATTG CCGGCCCCGC CATGAAGTCC GTGAATGCCC CGACGGCCGA AGTGAAGGGC AGGCCGCCAC CCAGGCCGCC GCCCTCACTG CCCGGCACCT GGTCGCTGAA TGTCGATGCC AGCACCTGCG GCACGTCAAT GCTTCCGGGC GTCGCGCTCG GGCTGATCGC CCATCCCGTT ACTGCCCCGA TCCCGGCAAT GGCAAGGACT GCCAGCGCTG CCATTTTTGG GGTGAGGCCG TTCGCGGCCG AGGGGCGCAG CCCCTGGGGG GATGGGAGGC CCGCGTTAGC GGGCCGGGAG GGTTCGAGAA GGGGGGGCAC CCCCCTTCGG CGTGCGCGGT CACGCGCACA GGGCGCAGCC CTGGTTAAAA ACAAGGTTTA TAAATATTGG TTTAAAAGCA GGTTAAAAGA CAGGTTAGCG GTGGCCGAAA AACGGGCGGA AACCCTTGCA AATGCTGGAT TTTCTGCCTG TGGACAGCCC CTCAAATGTC AATAGGTGCG CCCCTCATCT GTCAGCACTC TGCCCCTCAA GTGTCAAGGA TCGCGCCCCT CATCTGTCAG TAGTCGCGCC CCTCAAGTGT CAATACCGCA GGGCACTTAT CCCCAGGCTT GTCCACATCA TCTGTGGGAA ACTCGCGTAA AATCAGGCGT TTTCGCCGAT TTGCGAGGCT GGCCAGCTCC ACGTCGCCGG CCGAAATCGA GCCTGCCCCT CATCTGTCAA CGCCGCGCCG GGTGAGTCGG CCCCTCAAGT GTCAACGTCC GCCCCTCATC TGTCAGTGAG GGCCAAGTTT TCCGCGAGGT ATCCACAACG CCGGCGGCCG CGGTGTCTCG CACACGGCTT CGACGGCGTT TCTGGCGCGT TTGCAGGGCC ATAGACGGCC GCCAGCCCAG CGGCGAGGGC AACCAGCCCG G
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