pECMV-KIF15-3×FLAG
$84.00
In stock
SKU
PL85044
BC117174.1;HKLP2;NY-BR-62
100μg liquid plasmid(Instantly):$105
| name | pECMV-KIF15-3×FLAG | another name | apECMV-KIF15-3×FLAG |
| Insert Size (bp) | 3876bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F： | |
| 3′ sequencing primer | BGH： | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCTTGGTA CCGAGCTCGG ATCCACTAGT CCAGTGTGGT GGAATTCTGC AGATATCGCC ACCATGAGCG GTTATAATGG TACCATCTTT GCATATGGAC AGACTGGCTC AGGGAAGACA TTTACTATGA TGGGACCATC TGAATCTGAT AATTTTTCTC ATAACCTGAG AGGAGTAATC CCACGAAGTT TTGAATATTT GTTTTCCTTA ATTGATCGTG AAAAAGAAAA GGCTGGAGCT GGAAAGAGTT TCCTTTGTAA GTGTTCCTTT ATTGAAATCT ACAACGAGCA GATATATGAT CTACTGGACT CTGCATCGGC TGGACTGTAC TTAAGGGAGC ATATCAAGAA GGGAGTCTTT GTTGTTGGTG CGGTGGAGCA GGTGGTAACC TCAGCTGCTG AAGCCTATCA GGTGTTGTCT GGAGGATGGA GGAATAGACG TGTGGCATCA ACATCAATGA ACAGAGAATC GTCTAGGTCT CATGCCGTCT TTACAATTAC AATAGAGTCA ATGGAGAAAA GTAATGAGAT TGTGAATATA CGGACCTCCC TACTCAACCT GGTGGATTTA GCAGGATCTG AAAGGCAAAA AGATACCCAT GCAGAAGGGA TGAGATTGAA GGAAGCAGGT AACATAAATC GATCATTGAG CTGCCTGGGC CAAGTGATTA CAGCACTTGT CGACGTGGGT AATGGAAAAC AGAGACATGT TTGCTACAGA GACTCCAAAC TTACCTTCTT ACTACGGGAT TCCCTTGGAG GTAATGCCAA AACAGCCATA ATTGCAAATG TTCATCCTGG ATCCAGGTGT TTTGGGGAAA CCCTATCAAC ACTTAACTTT GCTCAAAGAG CCAAGCTGAT TAAAAACAAG GCAGTAGTAA ATGAAGACAC CCAAGGAAAT GTGAGCCAGC TCCAAGCTGA AGTGAAGAGG CTCAAAGAAC AACTGGCGGA GCTTGCTTCA GGACAGACAC CACCAGAAAG CTTCCTGACC AGAGACAAAA AGAAGACTAA CTATATGGAG TATTTCCAGG AAGCAATGTT ATTCTTTAAG AAATCTGAAC AGGAAAAGAA GTCTCTGATA GAAAAAGTTA CCCAATTAGA AGACCTCACC CTCAAAAAGG AAAAATTTAT TCAATCTAAT AAAATGATTG TGAAATTCCG AGAGGATCAA ATAATACGCT TGGAAAAGCT CCACAAGGAA TCCCGGGGAG GTTTTCTGCC TGAGGAGCAG GATCGTTTGC TCTCAGAATT AAGGAATGAG ATTCAAACTC TGCGAGAACA AATAGAGCAC CACCCCAGAG TTGCAAAGTA TGCTATGGAA AATCATTCCC TCAGGGAGGA GAATAGAAGA CTGAGATTAT TAGAGCCTGT GAAAAGAGCT CAAGAAATGG ATGCCCAGAC CATTGCAAAA CTAGAAAAAG CTTTCTCTGA AATAAGTGGC ATGGAGAAAA GTGACAAAAA TCAGCAAGGA TTTTCACCTA AAGCTCAGAA AGAGCCATGT TTGTTTGCAA ACACTGAGAA GTTAAAAGCA CAACTCCTGC AAATTCAGAC AGAGCTGAAT AATTCAAAGC AAGAATATGA AGAATTCAAA GAACTTACTA GGAAAAGGCA GCTAGAATTG GAATCAGAGC TTCAGTCTTT GCAAAAAGCG AACCTTAATC TTGAAAACCT TTTGGAAGCA ACAAAAGCCT GCAAGCGGCA AGAAGTTTCT CAGCTGAATA AAATTCATGC TGAAACACTT AAGATTATAA CTACACCAAC CAAGGCCTAC CAACTTCATT CCCGACCAGT ACCAAAATTA AGCCCTGAAA TGGGAAGCTT TGGCTCTCTA TACACTCAGA ATTCTAGCAT ATTAGATAAT GATATATTAA ATGAGCCAGT TCCTCCTGAG ATGAATGAAC AAGCTTTTGA GGCCATTTCT GAAGAGCTTA GAACAGTGCA GGAACAAATG AGTGCTCTTC AAGCCAAACT GGATGAAGAA GAGCATAAAA ACCTAAAGCT TCAGCAGCAT GTTGACAAAC TGGAACATCA TTCTACCCAA ATGCAGGAGC TTTTCTCATC AGAAAGAATT GATTGGACCA AACAGCAGGA AGAGCTTCTC TCACAGTTGA ATGTCCTTGA AAAGCAGCTT CAAGAGACTC AAACTAAAAA TGACTTTTTG AAAAGTGAGG TACATGACCT GCGAGTAGTC CTTCATTCTG CTGACAAGGA GCTTTCTTCA GTGAAATTGG AATATAGTTC ATTCAAAACG AATCAGGAGA AAGAATTCAA CAAACTTTCC GAAAGACACA TGCATGTACA GCTTCAATTA GATAATCTCA GGTTAGAAAA CGAAAAGCTG CTTGAGAGCA AAGCCTGCCT ACAGGATTCC TATGACAACT TACAAGAAAT AATGAAATTT GAGATTGACC AACTTTCAAG AAACCTCCAA AACTTCAAAA AAGAAAATGA AACTCTGAAA TCTGATCTGA ATAATTTGAT GGAGCTTCTT GAGGCAGAAA AAGAACGCAA TAACAAATTA TCATTACAGT TTGAAGAAGA TAAAGAAAAC AGTTCTAAAG AAATCTTAAA AGTTCTTGAG GCTGTACGTC AGGAGAAACA GAAAGAGACG GCCAAGTGTG AGCAGCAGAT GGCAAAAGTA CAGAAACTAG AAGAGAGCTT GCTTGCTACT GAAAAAGTGA TCAGTTCCCT GGAAAAGTCT AGAGATTCTG ATAAGAAAGT TGTAGCTGAC CTCATGAACC AGATCCAGGA GCTAAGATCA TCGGTCTGTG AGAAAACAGA AACTATAGAC ACCCTGAAAC AAGAACTGAA GGACATAAAT TGCAAATACA ACTCTGCTTT GGTTGACAGA GAAGAGAGCA GAGTGTTGAT CAAGAAGCAG GAAGTGGATA TTCTGGATCT GAAAGAAACC CTTAGGCTGA GAATACTTTC TGAGGACATA GAGAGGGATA TGCTCTGTGA GGACCTGGCT CATGCCACTG AGCAGCTGAA CATGCTCACA GAGGCCTCAA AAAAACACTC GGGGCTGCTG CAGTCTGCCC AGGAAGAACT GACCAAGAAG GAAGCCCTGA TTCAGGAACT TCAGCACAAG CTAAACCAAA AGAAAGAGGA AGTAGAACAG AAGAAGAATG AATATAACTT CAAAATGAGG CAACTAGAAC ATGTGATGGA TTCTGCTGCT GAGGATCCCC AGAGTCCTAA GACACCACCT CACTTTCAAA CACATTTGGC AAAACTCCTG GAAACACAAG AACAAGAGAT AGAAGATGGA AGAGCCTCTA AGACTTCTTT GGAACACCTT GTAACAAAGC TAAATGAAGA CAGAGAAGTC AAAAATGCTG AAATCCTCAG AATGAAGGAG CAGTTGCGTG AAATGGAAAA CCTACGCCTG GAAAGTCAGC AGTTAATAGA GAAAAACTGG CTCCTGCAAG GTCAGCTGGA TGATATTAAA AGACAAAAGG AAAACAGTGA TCAGAATCAT CCAGATAATC AACAGCTGAA GAATGAACAA GAAGAAAGTA TCAAAGAAAG ACTTGCAAAA AGTAAAATAG TTGAAGAAAT GCTGAAAATG AAAGCAGACC TAGAAGAAGT CCAAAGTGCC CTTTACAACA AAGAGATGGA ATGCCTTAGA ATGACTGATG AAGTCGAACG AACCCAAACT TTGGAGTCTA AAGCATTCCA GGAAAAAGAA CAACTGAGAT CAAAGCTGGA AGAAATGTAT GAAGAAAGAG AGAGAACATC CCAGGAGATG GAAATGTTAA GGAAGCAGGT GGAGTGTCTT GCTGAGGAAA ATGGAAAGTT GGTAGGTCAC CAAAATTTGC ATCAGAAGAT TCAGTACGTA GTGCGACTAA AGAAGGAAAA TGTCAGGCTT GCTGAGGAGA CAGAAAAGTT GCGTGCCGAA AATGTATTTT TAAAAGAAAA GAAAAGAAGT GAATCTCTCG AGGACTACAA AGACCATGAC GGTGATTATA AAGATCATGA CATCGACTAC AAGGATGACG ATGACAAGTA GTGAGGGCCC GTTTAAACCC GCTGATCAGC CTCGACTGTG CCTTCTAGTT GCCAGCCATC TGTTGTTTGC CCCTCCCCCG TGCCTTCCTT GACCCTGGAA GGTGCCACTC CCACTGTCCT TTCCTAATAA AATGAGGAAA TTGCATCGCA TTGTCTGAGT AGGTGTCATT CTATTCTGGG GGGTGGGGTG GGGCAGGACA GCAAGGGGGA GGATTGGGAA GACAATAGCA GGCATGCTGG GGATGCGGTG GGCTCTATGG CTTCTGAGGC GGAAAGAACC AGCTGGGGCT CTAGGGGGTA TCCCCACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTAATTCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCTGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGAAAT GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT CGACCTCTAG CTAGAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTC
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