pHelper
$55.00
In stock
SKU
PL84944
DB00008;
100μg liquid plasmid(Instantly):$72
| name | pHelper | another name | apHelper |
| Insert Size (bp) | Plasmid host | Mammary cell,Adenovirus | |
| Plasmid uses | packaging aid | Fragment type | |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pUC | |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | f1 ori-R：TGGCAAGTGTAGCGGTCAC | |
| 3′ sequencing primer | Forward(left ITR)：CCTCTGACTTGAGCGTCGAT | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GGTACCCAAC TCCATGCTTA ACAGTCCCCA GGTACAGCCC ACCCTGCGTC GCAACCAGGA ACAGCTCTAC AGCTTCCTGG AGCGCCACTC GCCCTACTTC CGCAGCCACA GTGCGCAGAT TAGGAGCGCC ACTTCTTTTT GTCACTTGAA AAACATGTAA AAATAATGTA CTAGGAGACA CTTTCAATAA AGGCAAATGT TTTTATTTGT ACACTCTCGG GTGATTATTT ACCCCCCACC CTTGCCGTCT GCGCCGTTTA AAAATCAAAG GGGTTCTGCC GCGCATCGCT ATGCGCCACT GGCAGGGACA CGTTGCGATA CTGGTGTTTA GTGCTCCACT TAAACTCAGG CACAACCATC CGCGGCAGCT CGGTGAAGTT TTCACTCCAC AGGCTGCGCA CCATCACCAA CGCGTTTAGC AGGTCGGGCG CCGATATCTT GAAGTCGCAG TTGGGGCCTC CGCCCTGCGC GCGCGAGTTG CGATACACAG GGTTGCAGCA CTGGAACACT ATCAGCGCCG GGTGGTGCAC GCTGGCCAGC ACGCTCTTGT CGGAGATCAG ATCCGCGTCC AGGTCCTCCG CGTTGCTCAG GGCGAACGGA GTCAACTTTG GTAGCTGCCT TCCCAAAAAG GGTGCATGCC CAGGCTTTGA GTTGCACTCG CACCGTAGTG GCATCAGAAG GTGACCGTGC CCGGTCTGGG CGTTAGGATA CAGCGCCTGC ATGAAAGCCT TGATCTGCTT AAAAGCCACC TGAGCCTTTG CGCCTTCAGA GAAGAACATG CCGCAAGACT TGCCGGAAAA CTGATTGGCC GGACAGGCCG CGTCATGCAC GCAGCACCTT GCGTCGGTGT TGGAGATCTG CACCACATTT CGGCCCCACC GGTTCTTCAC GATCTTGGCC TTGCTAGACT GCTCCTTCAG CGCGCGCTGC CCGTTTTCGC TCGTCACATC CATTTCAATC ACGTGCTCCT TATTTATCAT AATGCTCCCG TGTAGACACT TAAGCTCGCC TTCGATCTCA GCGCAGCGGT GCAGCCACAA CGCGCAGCCC GTGGGCTCGT GGTGCTTGTA GGTTACCTCT GCAAACGACT GCAGGTACGC CTGCAGGAAT CGCCCCATCA TCGTCACAAA GGTCTTGTTG CTGGTGAAGG TCAGCTGCAA CCCGCGGTGC TCCTCGTTTA GCCAGGTCTT GCATACGGCC GCCAGAGCTT CCACTTGGTC AGGCAGTAGC TTGAAGTTTG CCTTTAGATC GTTATCCACG TGGTACTTGT CCATCAACGC GCGCGCAGCC TCCATGCCCT TCTCCCACGC AGACACGATC GGCAGGCTCA GCGGGTTTAT CACCGTGCTT TCACTTTCCG CTTCACTGGA CTCTTCCTTT TCCTCTTGCG TCCGCATACC CCGCGCCACT GGGTCGTCTT CATTCAGCCG CCGCACCGTG CGCTTACCTC CCTTGCCGTG CTTGATTAGC ACCGGTGGGT TGCTGAAACC CACCATTTGT AGCGCCACAT CTTCTCTTTC TTCCTCGCTG TCCACGATCA CCTCTGGGGA TGGCGGGCGC TCGGGCTTGG GAGAGGGGCG CTTCTTTTTC TTTTTGGACG CAATGGCCAA ATCCGCCGTC GAGGTCGATG GCCGCGGGCT GGGTGTGCGC GGCACCAGCG CATCTTGTGA CGAGTCTTCT TCGTCCTCGG ACTCGAGACG CCGCCTCAGC CGCTTTTTTG GGGGCGCGCG GGGAGGCGGC GGCGACGGCG ACGGGGACGA CACGTCCTCC ATGGTTGGTG GACGTCGCGC CGCACCGCGT CCGCGCTCGG GGGTGGTTTC GCGCTGCTCC TCTTCCCGAC TGGCCATTTC CTTCTCCTAT AGGCAGAAAA AGATCATGGA GTCAGTCGAG AAGGAGGACA GCCTAACCGC CCCCTTTGAG TTCGCCACCA CCGCCTCCAC CGATGCCGCC AACGCGCCTA CCACCTTCCC CGTCGAGGCA CCCCCGCTTG AGGAGGAGGA AGTGATTATC GAGCAGGACC CAGGTTTTGT AAGCGAAGAC GACGAGGATC GCTCAGTACC AACAGAGGAT AAAAAGCAAG ACCAGGACGA CGCAGAGGCA AACGAGGAAC AAGTCGGGCG GGGGGACCAA AGGCATGGCG ACTACCTAGA TGTGGGAGAC GACGTGCTGT TGAAGCATCT GCAGCGCCAG TGCGCCATTA TCTGCGACGC GTTGCAAGAG CGCAGCGATG TGCCCCTCGC CATAGCGGAT GTCAGCCTTG CCTACGAACG CCACCTGTTC TCACCGCGCG TACCCCCCAA ACGCCAAGAA AACGGCACAT GCGAGCCCAA CCCGCGCCTC AACTTCTACC CCGTATTTGC CGTGCCAGAG GTGCTTGCCA CCTATCACAT CTTTTTCCAA AACTGCAAGA TACCCCTATC CTGCCGTGCC AACCGCAGCC GAGCGGACAA GCAGCTGGCC TTGCGGCAGG GCGCTGTCAT ACCTGATATC GCCTCGCTCG ACGAAGTGCC AAAAATCTTT GAGGGTCTTG GACGCGACGA GAAACGCGCG GCAAACGCTC TGCAACAAGA AAACAGCGAA AATGAAAGTC ACTGTGGAGT GCTGGTGGAA CTTGAGGGTG ACAACGCGCG CCTAGCCGTG CTGAAACGCA GCATCGAGGT CACCCACTTT GCCTACCCGG CACTTAACCT ACCCCCCAAG GTTATGAGCA CAGTCATGAG CGAGCTGATC GTGCGCCGTG CACGACCCCT GGAGAGGGAT GCAAACTTGC AAGAACAAAC CGAGGAGGGC CTACCCGCAG TTGGCGATGA GCAGCTGGCG CGCTGGCTTG AGACGCGCGA GCCTGCCGAC TTGGAGGAGC GACGCAAGCT AATGATGGCC GCAGTGCTTG TTACCGTGGA GCTTGAGTGC ATGCAGCGGT TCTTTGCTGA CCCGGAGATG CAGCGCAAGC TAGAGGAAAC GTTGCACTAC ACCTTTCGCC AGGGCTACGT GCGCCAGGCC TGCAAAATTT CCAACGTGGA GCTCTGCAAC CTGGTCTCCT ACCTTGGAAT TTTGCACGAA AACCGCCTCG GGCAAAACGT GCTTCATTCC ACGCTCAAGG GCGAGGCGCG CCGCGACTAC GTCCGCGACT GCGTTTACTT ATTTCTGTGC TACACCTGGC AAACGGCCAT GGGCGTGTGG CAGCAATGCC TGGAGGAGCG CAACCTAAAG GAGCTGCAGA AGCTGCTAAA GCAAAACTTG AAGGACCTAT GGACGGCCTT CAACGAGCGC TCCGTGGCCG CGCACCTGGC GGACATTATC TTCCCCGAAC GCCTGCTTAA AACCCTGCAA CAGGGTCTGC CAGACTTCAC CAGTCAAAGC ATGTTGCAAA ACTTTAGGAA CTTTATCCTA GAGCGTTCAG GAATTCTGCC CGCCACCTGC TGTGCGCTTC CTAGCGACTT TGTGCCCATT AAGTACCGTG AATGCCCTCC GCCGCTTTGG GGTCACTGCT ACCTTCTGCA GCTAGCCAAC TACCTTGCCT ACCACTCCGA CATCATGGAA GACGTGAGCG GTGACGGCCT ACTGGAGTGT CACTGTCGCT GCAACCTATG CACCCCGCAC CGCTCCCTGG TCTGCAATTC GCAACTGCTT AGCGAAAGTC AAATTATCGG TACCTTTGAG CTGCAGGGTC CCTCGCCTGA CGAAAAGTCC GCGGCTCCGG GGTTGAAACT CACTCCGGGG CTGTGGACGT CGGCTTACCT TCGCAAATTT GTACCTGAGG ACTACCACGC CCACGAGATT AGGTTCTACG AAGACCAATC CCGCCCGCCA AATGCGGAGC TTACCGCCTG CGTCATTACC CAGGGCCACA TCCTTGGCCA ATTGCAAGCC ATCAACAAAG CCCGCCAAGA GTTTCTGCTA CGAAAGGGAC GGGGGGTTTA CCTGGACCCC CAGTCCGGCG AGGAGCTCAA CCCAATCCCC CCGCCGCCGC AGCCCTATCA GCAGCCGCGG GCCCTTGCTT CCCAGGATGG CACCCAAAAA GAAGCTGCAG CTGCCGCCGC CGCCACCCAC GGACGAGGAG GAATACTGGG ACAGTCAGGC AGAGGAGGTT TTGGACGAGG AGGAGGAGAT GATGGAAGAC TGGGACAGCC TAGACGAAGC TTCCGAGGCC GAAGAGGTGT CAGACGAAAC ACCGTCACCC TCGGTCGCAT TCCCCTCGCC GGCGCCCCAG AAATTGGCAA CCGTTCCCAG CATCGCTACA ACCTCCGCTC CTCAGGCGCC GCCGGCACTG CCTGTTCGCC GACCCAACCG TAGATGGGAC ACCACTGGAA CCAGGGCCGG TAAGTCTAAG CAGCCGCCGC CGTTAGCCCA AGAGCAACAA CAGCGCCAAG GCTACCGCTC GTGGCGCGGG CACAAGAACG CCATAGTTGC TTGCTTGCAA GACTGTGGGG GCAACATCTC CTTCGCCCGC CGCTTTCTTC TCTACCATCA CGGCGTGGCC TTCCCCCGTA ACATCCTGCA TTACTACCGT CATCTCTACA GCCCCTACTG CACCGGCGGC AGCGGCAGCG GCAGCAACAG CAGCGGTCAC ACAGAAGCAA AGGCGACCGG ATAGCAAGAC TCTGACAAAG CCCAAGAAAT CCACAGCGGC GGCAGCAGCA GGAGGAGGAG CGCTGCGTCT GGCGCCCAAC GAACCCGTAT CGACCCGCGA GCTTAGAAAT AGGATTTTTC CCACTCTGTA TGCTATATTT CAACAAAGCA GGGGCCAAGA ACAAGAGCTG AAAATAAAAA ACAGGTCTCT GCGCTCCCTC ACCCGCAGCT GCCTGTATCA CAAAAGCGAA GATCAGCTTC GGCGCACGCT GGAAGACGCG GAGGCTCTCT TCAGCAAATA CTGCGCGCTG ACTCTTAAGG ACTAGTTTCG CGCCCTTTCT CAAATTTAAG CGCGAAAACT ACGTCATCTC CAGCGGCCAC ACCCGGCGCC AGCACCTGTC GTCAGCGCCA TTATGAGCAA GGAAATTCCC ACGCCCTACA TGTGGAGTTA CCAGCCACAA ATGGGACTTG CGGCTGGAGC TGCCCAAGAC TACTCAACCC GAATAAACTA CATGAGCGCG GGACCCCACA TGATATCCCG GGTCAACGGA ATCCGCGCCC ACCGAAACCG AATTCTCCTC GAACAGGCGG CTATTACCAC CACACCTCGT AATAACCTTA ATCCCCGTAG TTGGCCCGCT GCCCTGGTGT ACCAGGAAAG TCCCGCTCCC ACCACTGTGG TACTTCCCAG AGACGCCCAG GCCGAAGTTC AGATGACTAA CTCAGGGGCG CAGCTTGCGG GCGGCTTTCG TCACAGGGTG CGGTCGCCCG GGCGTTTTAG GGCGGAGTAA CTTGCATGTA TTGGGAATTG TAGTTTTTTT AAAATGGGAA GTGACGTATC GTGGGAAAAC GGAAGTGAAG ATTTGAGGAA GTTGTGGGTT TTTTGGCTTT CGTTTCTGGG CGTAGGTTCG CGTGCGGTTT TCTGGGTGTT TTTTGTGGAC TTTAACCGTT ACGTCATTTT TTAGTCCTAT ATATACTCGC TCTGTACTTG GCCCTTTTTA CACTGTGACT GATTGAGCTG GTGCCGTGTC GAGTGGTGTT TTTTAATAGG TTTTTTTACT GGTAAGGCTG ACTGTTATGG CTGCCGCTGT GGAAGCGCTG TATGTTGTTC TGGAGCGGGA GGGTGCTATT TTGCCTAGGC AGGAGGGTTT TTCAGGTGTT TATGTGTTTT TCTCTCCTAT TAATTTTGTT ATACCTCCTA TGGGGGCTGT AATGTTGTCT CTACGCCTGC GGGTATGTAT TCCCCCGGGC TATTTCGGTC GCTTTTTAGC ACTGACCGAT GTTAACCAAC CTGATGTGTT TACCGAGTCT TACATTATGA CTCCGGACAT GACCGAGGAA CTGTCGGTGG TGCTTTTTAA TCACGGTGAC CAGTTTTTTT ACGGTCACGC CGGCATGGCC GTAGTCCGTC TTATGCTTAT AAGGGTTGTT TTTCCTGTTG TAAGACAGGC TTCTAATGTT TAAATGTTTT TTTTTTTGTT ATTTTATTTT GTGTTTAATG CAGGAACCCG CAGACATGTT TGAGAGAAAA ATGGTGTCTT TTTCTGTGGT GGTTCCGGAA CTTACCTGCC TTTATCTGCA TGAGCATGAC TACGATGTGC TTGCTTTTTT GCGCGAGGCT TTGCCTGATT TTTTGAGCAG CACCTTGCAT TTTATATCGC CGCCCATGCA ACAAGCTTAC ATAGGGGCTA CGCTGGTTAG CATAGCTCCG AGTATGCGTG TCATAATCAG TGTGGGTTCT TTTGTCATGG TTCCTGGCGG GGAAGTGGCC GCGCTGGTCC GTGCAGACCT GCACGATTAT GTTCAGCTGG CCCTGCGAAG GGACCTACGG GATCGCGGTA TTTTTGTTAA TGTTCCGCTT TTGAATCTTA TACAGGTCTG TGAGGAACCT GAATTTTTGC AATCATGATT CGCTGCTTGA GGCTGAAGGT GGAGGGCGCT CTGGAGCAGA TTTTTACAAT GGCCGGACTT AATATTCGGG ATTTGCTTAG AGACATATTG ATAAGGTGGC GAGATGAAAA TTATTTGGGC ATGGTTGAAG GTGCTGGAAT GTTTATAGAG GAGATTCACC CTGAAGGGTT TAGCCTTTAC GTCCACTTGG ACGTGAGGGC AGTTTGCCTT TTGGAAGCCA TTGTGCAACA TCTTACAAAT GCCATTATCT GTTCTTTGGC TGTAGAGTTT GACCACGCCA CCGGAGGGGA GCGCGTTCAC TTAATAGATC TTCATTTTGA GGTTTTGGAT AATCTTTTGG AATAAAAAAA AAAAAACATG GTTCTTCCAG CTCTTCCCGC TCCTCCCGTG TGTGACTCGC AGAACGAATG TGTAGGTTGG CTGGGTGTGG CTTATTCTGC GGTGGTGGAT GTTATCAGGG CAGCGGCGCA TGAAGGAGTT TACATAGAAC CCGAAGCCAG GGGGCGCCTG GATGCTTTGA GAGAGTGGAT ATACTACAAC TACTACACAG AGCGAGCTAA GCGACGAGAC CGGAGACGCA GATCTGTTTG TCACGCCCGC ACCTGGTTTT GCTTCAGGAA ATATGACTAC GTCCGGCGTT CCATTTGGCA TGACACTACG ACCAACACGA TCTCGGTTGT CTCGGCGCAC TCCGTACAGT AGGGATCGCC TACCTCCTTT TGAGACAGAG ACCCGCGCTA CCATACTGGA GGATCATCCG CTGCTGCCCG AATGTAACAC TTTGACAATG CACAACGTGA GTTACGTGCG AGGTCTTCCC TGCAGTGTGG GATTTACGCT GATTCAGGAA TGGGTTGTTC CCTGGGATAT GGTTCTGACG CGGGAGGAGC TTGTAATCCT GAGGAAGTGT ATGCACGTGT GCCTGTGTTG TGCCAACATT GATATCATGA CGAGCATGAT GATCCATGGT TACGAGTCCT GGGCTCTCCA CTGTCATTGT TCCAGTCCCG GTTCCCTGCA GTGCATAGCC GGCGGGCAGG TTTTGGCCAG CTGGTTTAGG ATGGTGGTGG ATGGCGCCAT GTTTAATCAG AGGTTTATAT GGTACCGGGA GGTGGTGAAT TACAACATGC CAAAAGAGGT AATGTTTATG TCCAGCGTGT TTATGAGGGG TCGCCACTTA ATCTACCTGC GCTTGTGGTA TGATGGCCAC GTGGGTTCTG TGGTCCCCGC CATGAGCTTT GGATACAGCG CCTTGCACTG TGGGATTTTG AACAATATTG TGGTGCTGTG CTGCAGTTAC TGTGCTGATT TAAGTGAGAT CAGGGTGCGC TGCTGTGCCC GGAGGACAAG GCGTCTCATG CTGCGGGCGG TGCGAATCAT CGCTGAGGAG ACCACTGCCA TGTTGTATTC CTGCAGGACG GAGCGGCGGC GGCAGCAGTT TATTCGCGCG CTGCTGCAGC ACCACCGCCC TATCCTGATG CACGATTATG ACTCTACCCC CATGTAGGCG TGGACTTCCC CTTCGCCGCC CGTTGAGCAA CCGCAAGTTG GACAGCAGCC TGTGGCTCAG CAGCTGGACA GCGACATGAA CTTAAGCGAG CTGCCCGGGG AGTTTATTAA TATCACTGAT GAGCGTTTGG CTCGACAGGA AACCGTGTGG AATATAACAC CTAAGAATAT GTCTGTTACC CATGATATGA TGCTTTTTAA GGCCAGCCGG GGAGAAAGGA CTGTGTACTC TGTGTGTTGG GAGGGAGGTG GCAGGTTGAA TACTAGGGTT CTGTGAGTTT GATTAAGGTA CGGTGATCAA TATAAGCTAT GTGGTGGTGG GGCTATACTA CTGAATGAAA AATGACTTGA AATTTTCTGC AATTGAAAAA TAAACACGTT GAAACATAAC ATGCAACAGG TTCACGATTC TTTATTCCTG GGCAATGTAG GAGAAGGTGT AAGAGTTGGT AGCAAAAGTT TCAGTGGTGT ATTTTCCACT TTCCCAGGAC CATGTAAAAG ACATAGAGTA AGTGCTTACC TCGCTAGTTT CTGTGGATTC ACTAGAATCG ATGTAGGATG TTGCCCCTCC TGACGCGGTA GGAGAAGGGG AGGGTGCCCT GCATGTCTGC CGCTGCTCTT GCTCTTGCCG CTGCTGAGGA GGGGGGCGCA TCTGCCGCAG CACCGGATGC ATCTGGGAAA AGCAAAAAAG GGGCTCGTCC CTGTTTCCGG AGGAATTTGC AAGCGGGGTC TTGCATGACG GGGAGGCAAA CCCCCGTTCG CCGCAGTCCG GCCGGCCCGA GACTCGAACC GGGGGTCCTG CGACTCAACC CTTGGAAAAT AACCCTCCGG CTACAGGGAG CGAGCCACTT AATGCTTTCG CTTTCCAGCC TAACCGCTTA CGCCGCGCGC GGCCAGTGGC CAAAAAAGCT AGCGCAGCAG CCGCCGCGCC TGGAAGGAAG CCAAAAGGAG CGCTCCCCCG TTGTCTGACG TCGCACACCT GGGTTCGACA CGCGGGCGGT AACCGCATGG ATCACGGCGG ACGGCCGGAT CCGGGGTTCG AACCCCGGTC GTCCGCCATG ATACCCTTGC GAATTTATCC ACCAGACCAC GGAAGAGTGC CCGCTTACAG GCTCTCCTTT TGCACGGTCT AGAGCGTCAA CGACTGCGCA CGCCTCACCG GCCAGAGCGT CCCGACCATG GAGCACTTTT TGCCGCTGCG CAACATCTGG AACCGCGTCC GCGACTTTCC GCGCGCCTCC ACCACCGCCG CCGGCATCAC CTGGATGTCC AGGTACATCT ACGGATTACG TCGACGTTTA AACCATATGA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTAAATTGTA AGCGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA GATAGGGTTG AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC CACACCCGCC GCGCTTAATG CGCCGCTACA GGGCGCGATG GATCC
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