pLP1
$55.00
In stock
SKU
PL84931
DB00035;pLP-1;pGag/Pol
100μg liquid plasmid(Instantly):$57
| name | pLP1 | another name | apLP1 |
| Insert Size (bp) | Plasmid host | Mammalian cell,Lentivirus | |
| Plasmid uses | packaging aid | Fragment type | ORF |
| Fragment species | Virus | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | Forward(left ITR)：CCTCTGACTTGAGCGTCGAT | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TTGGCCCATT GCATACGTTG TATCCATATC ATAATATGTA CATTTATATT GGCTCATGTC CAACATTACC GCCATGTTGA CATTGATTAT TGACTAGTTA TTAATAGTAA TCAATTACGG GGTCATTAGT TCATAGCCCA TATATGGAGT TCCGCGTTAC ATAACTTACG GTAAATGGCC CGCCTGGCTG ACCGCCCAAC GACCCCCGCC CATTGACGTC AATAATGACG TATGTTCCCA TAGTAACGCC AATAGGGACT TTCCATTGAC GTCAATGGGT GGAGTATTTA CGGTAAACTG CCCACTTGGC AGTACATCAA GTGTATCATA TGCCAAGTAC GCCCCCTATT GACGTCAATG ACGGTAAATG GCCCGCCTGG CATTATGCCC AGTACATGAC CTTATGGGAC TTTCCTACTT GGCAGTACAT CTACGTATTA GTCATCGCTA TTACCATGGT GATGCGGTTT TGGCAGTACA TCAATGGGCG TGGATAGCGG TTTGACTCAC GGGGATTTCC AAGTCTCCAC CCCATTGACG TCAATGGGAG TTTGTTTTGG CACCAAAATC AACGGGACTT TCCAAAATGT CGTAACAACT CCGCCCCATT GACGCAAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT ATAAGCAGAG CTCGTTTAGT GAACCGTCAG ATCGCCTGGA GACGCCATCC ACGCTGTTTT GACCTCCATA GAAGACACCG GGACCGATCC AGCCTCCCCT CGAAGCTTAC ATGTGGTACC GAGCTCGGAT CCTGAGAACT TCAGGGTGAG TCTATGGGAC CCTTGATGTT TTCTTTCCCC TTCTTTTCTA TGGTTAAGTT CATGTCATAG GAAGGGGAGA AGTAACAGGG TACACATATT GACCAAATCA GGGTAATTTT GCATTTGTAA TTTTAAAAAA TGCTTTCTTC TTTTAATATA CTTTTTTGTT TATCTTATTT CTAATACTTT CCCTAATCTC TTTCTTTCAG GGCAATAATG ATACAATGTA TCATGCCTCT TTGCACCATT CTAAAGAATA ACAGTGATAA TTTCTGGGTT AAGGCAATAG CAATATTTCT GCATATAAAT ATTTCTGCAT ATAAATTGTA ACTGATGTAA GAGGTTTCAT ATTGCTAATA GCAGCTACAA TCCAGCTACC ATTCTGCTTT TATTTTATGG TTGGGATAAG GCTGGATTAT TCTGAGTCCA AGCTAGGCCC TTTTGCTAAT CATGTTCATA CCTCTTATCT TCCTCCCACA GCTCCTGGGC AACGTGCTGG TCTGTGTGCT GGCCCATCAC TTTGGCAAAG CACGTGAGAT CTGAATTCGA GATCTGCCGC CGCCATGGGT GCGAGAGCGT CAGTATTAAG CGGGGGAGAA TTAGATCGAT GGGAAAAAAT TCGGTTAAGG CCAGGGGGAA AGAAAAAATA TAAATTAAAA CATATAGTAT GGGCAAGCAG GGAGCTAGAA CGATTCGCAG TTAATCCTGG CCTGTTAGAA ACATCAGAAG GCTGTAGACA AATACTGGGA CAGCTACAAC CATCCCTTCA GACAGGATCA GAAGAACTTA GATCATTATA TAATACAGTA GCAACCCTCT ATTGTGTGCA TCAAAGGATA GAGATAAAAG ACACCAAGGA AGCTTTAGAC AAGATAGAGG AAGAGCAAAA CAAAAGTAAG AAAAAAGCAC AGCAAGCAGC AGCTGACACA GGACACAGCA ATCAGGTCAG CCAAAATTAC CCTATAGTGC AGAACATCCA GGGGCAAATG GTACATCAGG CCATATCACC TAGAACTTTA AATGCATGGG TAAAAGTAGT AGAAGAGAAG GCTTTCAGCC CAGAAGTGAT ACCCATGTTT TCAGCATTAT CAGAAGGAGC CACCCCACAA GATTTAAACA CCATGCTAAA CACAGTGGGG GGACATCAAG CAGCCATGCA AATGTTAAAA GAGACCATCA ATGAGGAAGC TGCAGAATGG GATAGAGTGC ATCCAGTGCA TGCAGGGCCT ATTGCACCAG GCCAGATGAG AGAACCAAGG GGAAGTGACA TAGCAGGAAC TACTAGTACC CTTCAGGAAC AAATAGGATG GATGACACAT AATCCACCTA TCCCAGTAGG AGAAATCTAT AAAAGATGGA TAATCCTGGG ATTAAATAAA ATAGTAAGAA TGTATAGCCC TACCAGCATT CTGGACATAA GACAAGGACC AAAGGAACCC TTTAGAGACT ATGTAGACCG ATTCTATAAA ACTCTAAGAG CCGAGCAAGC TTCACAAGAG GTAAAAAATT GGATGACAGA AACCTTGTTG GTCCAAAATG CGAACCCAGA TTGTAAGACT ATTTTAAAAG CATTGGGACC AGGAGCGACA CTAGAAGAAA TGATGACAGC ATGTCAGGGA GTGGGGGGAC CCGGCCATAA AGCAAGAGTT TTGGCTGAAG CAATGAGCCA AGTAACAAAT CCAGCTACCA TAATGATACA GAAAGGCAAT TTTAGGAACC AAAGAAAGAC TGTTAAGTGT TTCAATTGTG GCAAAGAAGG GCACATAGCC AAAAATTGCA GGGCCCCTAG GAAAAAGGGC TGTTGGAAAT GTGGAAAGGA AGGACACCAA ATGAAAGATT GTACTGAGAG ACAGGCTAAT TTTTTAGGGA AGATCTGGCC TTCCCACAAG GGAAGGCCAG GGAATTTTCT TCAGAGCAGA CCAGAGCCAA CAGCCCCACC AGAAGAGAGC TTCAGGTTTG GGGAAGAGAC AACAACTCCC TCTCAGAAGC AGGAGCCGAT AGACAAGGAA CTGTATCCTT TAGCTTCCCT CAGATCACTC TTTGGCAGCG ACCCCTCGTC ACAATAAAGA TAGGGGGGCA ATTAAAGGAA GCTCTATTAG ATACAGGAGC AGATGATACA GTATTAGAAG AAATGAATTT GCCAGGAAGA TGGAAACCAA AAATGATAGG GGGAATTGGA GGTTTTATCA AAGTAAGACA GTATGATCAG ATACTCATAG AAATCTGCGG ACATAAAGCT ATAGGTACAG TATTAGTAGG ACCTACACCT GTCAACATAA TTGGAAGAAA TCTGTTGACT CAGATTGGCT GCACTTTAAA TTTTCCCATT AGTCCTATTG AGACTGTACC AGTAAAATTA AAGCCAGGAA TGGATGGCCC AAAAGTTAAA CAATGGCCAT TGACAGAAGA AAAAATAAAA GCATTAGTAG AAATTTGTAC AGAAATGGAA AAGGAAGGAA AAATTTCAAA AATTGGGCCT GAAAATCCAT ACAATACTCC AGTATTTGCC ATAAAGAAAA AAGACAGTAC TAAATGGAGA AAATTAGTAG ATTTCAGAGA ACTTAATAAG AGAACTCAAG ATTTCTGGGA AGTTCAATTA GGAATACCAC ATCCTGCAGG GTTAAAACAG AAAAAATCAG TAACAGTACT GGATGTGGGC GATGCATATT TTTCAGTTCC CTTAGATAAA GACTTCAGGA AGTATACTGC ATTTACCATA CCTAGTATAA ACAATGAGAC ACCAGGGATT AGATATCAGT ACAATGTGCT TCCACAGGGA TGGAAAGGAT CACCAGCAAT ATTCCAGTGT AGCATGACAA AAATCTTAGA GCCTTTTAGA AAACAAAATC CAGACATAGT CATCTATCAA TACATGGATG ATTTGTATGT AGGATCTGAC TTAGAAATAG GGCAGCATAG AACAAAAATA GAGGAACTGA GACAACATCT GTTGAGGTGG GGATTTACCA CACCAGACAA AAAACATCAG AAAGAACCTC CATTCCTTTG GATGGGTTAT GAACTCCATC CTGATAAATG GACAGTACAG CCTATAGTGC TGCCAGAAAA GGACAGCTGG ACTGTCAATG ACATACAGAA ATTAGTGGGA AAATTGAATT GGGCAAGTCA GATTTATGCA GGGATTAAAG TAAGGCAATT ATGTAAACTT CTTAGGGGAA CCAAAGCACT AACAGAAGTA GTACCACTAA CAGAAGAAGC AGAGCTAGAA CTGGCAGAAA ACAGGGAGAT TCTAAAAGAA CCGGTACATG GAGTGTATTA TGACCCATCA AAAGACTTAA TAGCAGAAAT ACAGAAGCAG GGGCAAGGCC AATGGACATA TCAAATTTAT CAAGAGCCAT TTAAAAATCT GAAAACAGGA AAGTATGCAA GAATGAAGGG TGCCCACACT AATGATGTGA AACAATTAAC AGAGGCAGTA CAAAAAATAG CCACAGAAAG CATAGTAATA TGGGGAAAGA CTCCTAAATT TAAATTACCC ATACAAAAGG AAACATGGGA AGCATGGTGG ACAGAGTATT GGCAAGCCAC CTGGATTCCT GAGTGGGAGT TTGTCAATAC CCCTCCCTTA GTGAAGTTAT GGTACCAGTT AGAGAAAGAA CCCATAATAG GAGCAGAAAC TTTCTATGTA GATGGGGCAG CCAATAGGGA AACTAAATTA GGAAAAGCAG GATATGTAAC TGACAGAGGA AGACAAAAAG TTGTCCCCCT AACGGACACA ACAAATCAGA AGACTGAGTT ACAAGCAATT CATCTAGCTT TGCAGGATTC GGGATTAGAA GTAAACATAG TGACAGACTC ACAATATGCA TTGGGAATCA TTCAAGCACA ACCAGATAAG AGTGAATCAG AGTTAGTCAG TCAAATAATA GAGCAGTTAA TAAAAAAGGA AAAAGTCTAC CTGGCATGGG TACCAGCACA CAAAGGAATT GGAGGAAATG AACAAGTAGA TAAATTGGTC AGTGCTGGAA TCAGGAAAGT ACTATTTTTA GATGGAATAG ATAAGGCCCA AGAAGAACAT GAGAAATATC ACAGTAATTG GAGAGCAATG GCTAGTGATT TTAACCTACC ACCTGTAGTA GCAAAAGAAA TAGTAGCCAG CTGTGATAAA TGTCAGCTAA AAGGGGAAGC CATGCATGGA CAAGTAGACT GTAGCCCAGG AATATGGCAG CTAGATTGTA CACATTTAGA AGGAAAAGTT ATCTTGGTAG CAGTTCATGT AGCCAGTGGA TATATAGAAG CAGAAGTAAT TCCAGCAGAG ACAGGGCAAG AAACAGCATA CTTCCTCTTA AAATTAGCAG GAAGATGGCC AGTAAAAACA GTACATACAG ACAATGGCAG CAATTTCACC AGTACTACAG TTAAGGCCGC CTGTTGGTGG GCGGGGATCA AGCAGGAATT TGGCATTCCC TACAATCCCC AAAGTCAAGG AGTAATAGAA TCTATGAATA AAGAATTAAA GAAAATTATA GGACAGGTAA GAGATCAGGC TGAACATCTT AAGACAGCAG TACAAATGGC AGTATTCATC CACAATTTTA AAAGAAAAGG GGGGATTGGG GGGTACAGTG CAGGGGAAAG AATAGTAGAC ATAATAGCAA CAGACATACA AACTAAAGAA TTACAAAAAC AAATTACAAA AATTCAAAAT TTTCGGGTTT ATTACAGGGA CAGCAGAGAT CCAGTTTGGA AAGGACCAGC AAAGCTCCTC TGGAAAGGTG AAGGGGCAGT AGTAATACAA GATAATAGTG ACATAAAAGT AGTGCCAAGA AGAAAAGCAA AGATCATCAG GGATTATGGA AAACAGATGG CAGGTGATGA TTGTGTGGCA AGTAGACAGG ATGAGGATTA ACACATGGAA TTCCGGAGCG GCCGCAGGAG CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCG TCAATGACGC TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA AATCTCTGGA ACAGATTTGG AATCACACGA CCTGGATGGA GTGGGACAGA GAAATTAACA ATTACACAAG CTTCCGCGGA ATTCACCCCA CCAGTGCAGG CTGCCTATCA GAAAGTGGTG GCTGGTGTGG CTAATGCCCT GGCCCACAAG TATCACTAAG CTCGCTTTCT TGCTGTCCAA TTTCTATTAA AGGTTCCTTT GTTCCCTAAG TCCAACTACT AAACTGGGGG ATATTATGAA GGGCCTTGAG CATCTGGATT CTGCCTAATA AAAAACATTT ATTTTCATTG CAATGATGTA TTTAAATTAT TTCTGAATAT TTTACTAAAA AGGGAATGTG GGAGGTCAGT GCATTTAAAA CATAAAGAAA TGAAGAGCTA GTTCAAACCT TGGGAAAATA CACTATATCT TAAACTCCAT GAAAGAAGGT GAGGCTGCAA ACAGCTAATG CACATTGGCA ACAGCCCCTG ATGCCTATGC CTTATTCATC CCTCAGAAAA GGATTCAAGT AGAGGCTTGA TTTGGAGGTT AAAGTTTTGC TATGCTGTAT TTTACATTAC TTATTGTTTT AGCTGTCCTC ATGAATGTCT TTTCACTACC CATTTGCTTA TCCTGCATCT CTCAGCCTTG ACTCCACTCA GTTCTCTTGC TTAGAGATAC CACCTTTCCC CTGAAGTGTT CCTTCCATGT TTTACGGCGA GATGGTTTCT CCTCGCCTGG CCACTCAGCC TTAGTTGTCT CTGTTGTCTT ATAGAGGTCT ACTTGAAGAA GGAAAAACAG GGGGCATGGT TTGACTGTCC TGTGAGCCCT TCTTCCCTGC CTCCCCCACT CACAGTGACC CGGAATCCCT CGACATGGCA GTCTAGCACT AGTGCGGCCG CAGATCTGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGGGA TCCCCTGAGG GGGCCCCCAT GGGCTAGAGG ATCCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGAGC
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