pGBKT7-53
$55.00
In stock
SKU
PL84871
DB00110;
100μg liquid plasmid(Instantly):$57
| name | pGBKT7-53 | another name | apGBKT7-53 |
| Insert Size (bp) | Plasmid host | Yeast bacterium | |
| Plasmid uses | hybridization | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | TRP1 | Fluorescent labeling | |
| Promoter | ADH1 | Replicon | 2U |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | T7：TAATACGACTCACTATAGGG | |
| 3′ sequencing primer | ADH1-R：GAGTCACTTTAAAATTTGTATACAC | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CGGTGCGGGC CTCTTCGCTA TTACGCCAGA TCCTTTTGTT GTTTCCGGGT GTACAATATG GACTTCCTCT TTTCTGGCAA CCAAACCCAT ACATCGGGAT TCCTATAATA CCTTCGTTGG TCTCCCTAAC ATGTAGGTGG CGGAGGGGAG ATATACAATA GAACAGATAC CAGACAAGAC ATAATGGGCT AAACAAGACT ACACCAATTA CACTGCCTCA TTGATGGTGG TACATAACGA ACTAATACTG TAGCCCTAGA CTTGATAGCC ATCATCATAT CGAAGTTTCA CTACCCTTTT TCCATTTGCC ATCTATTGAA GTAATAATAG GCGCATGCAA CTTCTTTTCT TTTTTTTTCT TTTCTCTCTC CCCCGTTGTT GTCTCACCAT ATCCGCAATG ACAAAAAAAA TGATGGAAGA CACTAAAGGA AAAAATTAAC GACAAAGACA GCACCAACAG ATGTCGTTGT TCCAGAGCTG ATGAGGGGTA TCTCGAAGCA CACGAAACTT TTTCCTTCCT TCATTCACGC ACACTACTCT CTAATGAGCA ACGGTATACG GCCTTCCTTC CAGTTACTTG AATTTGAAAT AAAAAAAGTT TGCTGTCTTG CTATCAAGTA TAAATAGACC TGCAATTATT AATCTTTTGT TTCCTCGTCA TTGTTCTCGT TCCCTTTCTT CCTTGTTTCT TTTTCTGCAC AATATTTCAA GCTATACCAA GCATACAATC AACTCCAAGC TTGAAGCAAG CCTCCTGAAA GATGAAGCTA CTGTCTTCTA TCGAACAAGC ATGCGATATT TGCCGACTTA AAAAGCTCAA GTGCTCCAAA GAAAAACCGA AGTGCGCCAA GTGTCTGAAG AACAACTGGG AGTGTCGCTA CTCTCCCAAA ACCAAAAGGT CTCCGCTGAC TAGGGCACAT CTGACAGAAG TGGAATCAAG GCTAGAAAGA CTGGAACAGC TATTTCTACT GATTTTTCCT CGAGAAGACC TTGACATGAT TTTGAAAATG GATTCTTTAC AGGATATAAA AGCATTGTTA ACAGGATTAT TTGTACAAGA TAATGTGAAT AAAGATGCCG TCACAGATAG ATTGGCTTCA GTGGAGACTG ATATGCCTCT AACATTGAGA CAGCATAGAA TAAGTGCGAC ATCATCATCG GAAGAGAGTA GTAACAAAGG TCAAAGACAG TTGACTGTAT CGCCGGAATT TGTAATACGA CTCACTATAG GGCGAGCCGC CATCATGGAG GAGCAGAAGC TGATCTCAGA GGAGGACCTG CATATGGCCA TGGAGGCCGA ATTCCCTGTC ACCGAGACCC CTGGGCCAGT GGCCCCTGCC CCAGCCACTC CATGGCCCCT GTCATCTTTT GTCCCTTCTC AAAAAACTTA CCAGGGCAAC TATGGCTTCC ACCTGGGCTT CCTGCAGTCT GGGACAGCCA AGTCTGTTAT GTGCACGTAC TCTCCTCCCC TCAATAAGCT ATTCTGCCAG CTGGCGAAGA CGTGCCCTGT GCAGTTGTGG GTCAGCGCCA CACCTCCAGC TGGGAGCCGT GTCCGCGCCA TGGCCATCTA CAAGAAGTCA CAGCACATGA CGGAGGTCGT GAGACGCTGC CCCCACCATG AGCGCTGCTC CGATGGTGAT GGCCTGGCTC CTCCCCAGCA TCTTATCCGG GTGGAAGGAA ATTTGTATCC CGAGTATCTG GAAGACAGGC AGACTTTTCG CCACAGCGTG GTGGTACCTT ATGAGCCACC CGAGGCCGGC TCTGAGTATA CCACCATCCA CTACAAGTAC ATGTGTAATA GCTCCTGCAT GGGGGGCATG AACCGCCGAC CTATCCTTAC CATCATCACA CTGGAAGACT CCAGTGGGAA CCTTCTGGGA CGGGACAGCT TTGAGGTTCG TGTTTGTGCC TGCCCTGGGA GAGACCGCCG TACAGAAGAA GAAAATTTCC GCAAAAAGGA AGTCCTTTGC CCTGAACTGC CCCCAGGGAG CGCAAAGAGA GCGCTGCCCA CCTGCACAAG CGCCTCTCCC CCGCAAAAGA AAAAACCACT TGATGGAGAG TATTTCACCC TCAAGATCCG CGGGCGTAAA CGCTTCGAGA TGTTCCGGGA GCTGAATGAG GCCTTAGAGT TAAAGGATGC CCATGCTACA GAGGAGTCTG GAGACAGCAG GGCTCACTCC AGCTACCTGA AGACCAAGAA GGGCCAGTCT ACTTCCCGCC ATAAAAAAAC AATGGTCAAG AAAGTGGGGC CTGACTCAGA CTGACTGCCT CTGCATCCCG GATCCGTCGA CCTGCAGCGG CCGCATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCGCG CTTGCAGCCA AGCTAATTCC GGGCGAATTT CTTATGATTT ATGATTTTTA TTATTAAATA AGTTATAAAA AAAATAAGTG TATACAAATT TTAAAGTGAC TCTTAGGTTT TAAAACGAAA ATTCTTATTC TTGAGTAACT CTTTCCTGTA GGTCAGGTTG CTTTCTCAGG TATAGCATGA GGTCGCTCTT ATTGACCACA CCTCTACCGG CATGCAAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGG TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GTCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAA CGAAGCATCT GTGCTTCATT TTGTAGAACA AAAATGCAAC GCGAGAGCGC TAATTTTTCA AACAAAGAAT CTGAGCTGCA TTTTTACAGA ACAGAAATGC AACGCGAAAG CGCTATTTTA CCAACGAAGA ATCTGTGCTT CATTTTTGTA AAACAAAAAT GCAACGCGAG AGCGCTAATT TTTCAAACAA AGAATCTGAG CTGCATTTTT ACAGAACAGA AATGCAACGC GAGAGCGCTA TTTTACCAAC AAAGAATCTA TACTTCTTTT TTGTTCTACA AAAATGCATC CCGAGAGCGC TATTTTTCTA ACAAAGCATC TTAGATTACT TTTTTTCTCC TTTGTGCGCT CTATAATGCA GTCTCTTGAT AACTTTTTGC ACTGTAGGTC CGTTAAGGTT AGAAGAAGGC TACTTTGGTG TCTATTTTCT CTTCCATAAA AAAAGCCTGA CTCCACTTCC CGCGTTTACT GATTACTAGC GAAGCTGCGG GTGCATTTTT TCAAGATAAA GGCATCCCCG ATTATATTCT ATACCGATGT GGATTGCGCA TACTTTGTGA ACAGAAAGTG ATAGCGTTGA TGATTCTTCA TTGGTCAGAA AATTATGAAC GGTTTCTTCT ATTTTGTCTC TATATACTAC GTATAGGAAA TGTTTACATT TTCGTATTGT TTTCGATTCA CTCTATGAAT AGTTCTTACT ACAATTTTTT TGTCTAAAGA GTAATACTAG AGATAAACAT AAAAAATGTA GAGGTCGAGT TTAGATGCAA GTTCAAGGAG CGAAAGGTGG ATGGGTAGGT TATATAGGGA TATAGCACAG AGATATATAG CAAAGAGATA CTTTTGAGCA ATGTTTGTGG AAGCGGTATT CGCAATATTT TAGTAGCTCG TTACAGTCCG GTGCGTTTTT GGTTTTTTGA AAGTGCGTCT TCAGAGCGCT TTTGGTTTTC AAAAGCGCTC TGAAGTTCCT ATACTTTCTA GAGAATAGGA ACTTCGGAAT AGGAACTTCA AAGCGTTTCC GAAAACGAGC GCTTCCGAAA ATGCAACGCG AGCTGCGCAC ATACAGCTCA CTGTTCACGT CGCACCTATA TCTGCGTGTT GCCTGTATAT ATATATACAT GAGAAGAACG GCATAGTGCG TGTTTATGCT TAAATGCGTA CTTATATGCG TCTATTTATG TAGGATGAAA GGTAGTCTAG TACCTCCTGT GATATTATCC CATTCCATGC GGGGTATCGT ATGCTTCCTT CAGCACTACC CTTTAGCTGT TCTATATGCT GCCACTCCTC AATTGGATTA GTCTCATCCT TCAATGCTAT CATTTCCTTT GATATTGGAT CATATTAAGA AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC CCTTTCGTCT CGCGCGTTTC GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT CGGGGCTGGC TTAACTATGC GGCATCAGAG CAGATTGTAC TGAGAGTGCA CCATAGATCA ACGACATTAC TATATATATA ATATAGGAAG CATTTAATAG AACAGCATCG TAATATATGT GTACTTTGCA GTTATGACGC CAGATGGCAG TAGTGGAAGA TATTCTTTAT TGAAAAATAG CTTGTCACCT TACGTACAAT CTTGATCCGG AGCTTTTCTT TTTTTGCCGA TTAAGAATTA ATTCGGTCGA AAAAAGAAAA GGAGAGGGCC AAGAGGGAGG GCATTGGTGA CTATTGAGCA CGTGAGTATA CGTGATTAAG CACACAAAGG CAGCTTGGAG TATGTCTGTT ATTAATTTCA CAGGTAGTTC TGGTCCATTG GTGAAAGTTT GCGGCTTGCA GAGCACAGAG GCCGCAGAAT GTGCTCTAGA TTCCGATGCT GACTTGCTGG GTATTATATG TGTGCCCAAT AGAAAGAGAA CAATTGACCC GGTTATTGCA AGGAAAATTT CAAGTCTTGT AAAAGCATAT AAAAATAGTT CAGGCACTCC GAAATACTTG GTTGGCGTGT TTCGTAATCA ACCTAAGGAG GATGTTTTGG CTCTGGTCAA TGATTACGGC ATTGATATCG TCCAACTGCA TGGAGATGAG TCGTGGCAAG AATACCAAGA GTTCCTCGGT TTGCCAGTTA TTAAAAGACT CGTATTTCCA AAAGACTGCA ACATACTACT CAGTGCAGCT TCACAGAAAC CTCATTCGTT TATTCCCTTG TTTGATTCAG AAGCAGGTGG GACAGGTGAA CTTTTGGATT GGAACTCGAT TTCTGACTGG GTTGGAAGGC AAGAGAGCCC CGAAAGCTTA CATTTTATGT TAGCTGGTGG ACTGACGCCA GAAAATGTTG GTGATGCGCT TAGATTAAAT GGCGTTATTG GTGTTGATGT AAGCGGAGGT GTGGAGACAA ATGGTGTAAA AGACTCTAAC AAAATAGCAA ATTTCGTCAA AAATGCTAAG AAATAGGTTA TTACTGAGTA GTATTTATTT AAGTATTGTT TGTGCACTTG CCGATCTATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGAA ATTGTAAACG TTAATATTTT GTTAAAATTC GCGTTAAATT TTTGTTAAAT CAGCTCATTT TTTAACCAAT AGGCCGAAAT CGGCAAAATC CCTTATAAAT CAAAAGAATA GACCGAGATA GGGTTGAGTG TTGTTCCAGT TTGGAACAAG AGTCCACTAT TAAAGAACGT GGACTCCAAC GTCAAAGGGC GAAAAACCGT CTATCAGGGC GATGGCCCAC TACGTGAACC ATCACCCTAA TCAAGTTTTT TGGGGTCGAG GTGCCGTAAA GCACTAAATC GGAACCCTAA AGGGAGCCCC CGATTTAGAG CTTGACGGGG AAAGCCGGCG AACGTGGCGA GAAAGGAAGG GAAGAAAGCG AAAGGAGCGG GCGCTAGGGC GCTGGCAAGT GTAGCGGTCA CGCTGCGCGT AACCACCACA CCCGCCGCGC TTAATGCGCC GCTACAGGGC GCGTCCATTC GCCATTCAGG CTGCGCAACT GTTGGGAAGG GCGAT
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