pGADT7-T
$55.00
In stock
SKU
PL84869
DB00090;
100μg liquid plasmid(Instantly):$57
| name | pGADT7-T | another name | apGADT7-T |
| Insert Size (bp) | Plasmid host | Yeast bacterium | |
| Plasmid uses | hybridization | Fragment type | |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | LEU2 | Fluorescent labeling | |
| Promoter | ADH1 | Replicon | 2U |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | T7：TAATACGACTCACTATAGGG | |
| 3′ sequencing primer | ADH1-R：GAGTCACTTTAAAATTTGTATACAC | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGCATGCCTG CAGGTCGAGA TCCGGGATCG AAGAAATGAT GGTAAATGAA ATAGGAAATC AAGGAGCATG AAGGCAAAAG ACAAATATAA GGGTCGAACG AAAAATAAAG TGAAAAGTGT TGATATGATG TATTTGGCTT TGCGGCGCCG AAAAAACGAG TTTACGCAAT TGCACAATCA TGCTGACTCT GTGGCGGACC CGCGCTCTTG CCGGCCCGGC GATAACGCTG GGCGTGAGGC TGTGCCCGGC GGAGTTTTTT GCGCCTGCAT TTTCCAAGGT TTACCCTGCG CTAAGGGGCG AGATTGGAGA AGCAATAAGA ATGCCGGTTG GGGTTGCGAT GATGACGACC ACGACAACTG GTGTCATTAT TTAAGTTGCC GAAAGAACCT GAGTGCATTT GCAACATGAG TATACTAGAA GAATGAGCCA AGACTTGCGA GACGCGAGTT TGCCGGTGGT GCGAACAATA GAGCGACCAT GACCTTGAAG GTGAGACGCG CATAACCGCT AGAGTACTTT GAAGAGGAAA CAGCAATAGG GTTGCTACCA GTATAAATAG ACAGGTACAT ACAACACTGG AAATGGTTGT CTGTTTGAGT ACGCTTTCAA TTCATTTGGG TGTGCACTTT ATTATGTTAC AATATGGAAG GGAACTTTAC ACTTCTCCTA TGCACATATA TTAATTAAAG TCCAATGCTA GTAGAGAAGG GGGGTAACAC CCCTCCGCGC TCTTTTCCGA TTTTTTTCTA AACCGTGGAA TATTTCGGAT ATCCTTTTGT TGTTTCCGGG TGTACAATAT GGACTTCCTC TTTTCTGGCA ACCAAACCCA TACATCGGGA TTCCTATAAT ACCTTCGTTG GTCTCCCTAA CATGTAGGTG GCGGAGGGGA GATATACAAT AGAACAGATA CCAGACAAGA CATAATGGGC TAAACAAGAC TACACCAATT ACACTGCCTC ATTGATGGTG GTACATAACG AACTAATACT GTAGCCCTAG ACTTGATAGC CATCATCATA TCGAAGTTTC ACTACCCTTT TTCCATTTGC CATCTATTGA AGTAATAATA GGCGCATGCA ACTTCTTTTC TTTTTTTTTC TTTTCTCTCT CCCCCGTTGT TGTCTCACCA TATCCGCAAT GACAAAAAAA ATGATGGAAG ACACTAAAGG AAAAAATTAA CGACAAAGAC AGCACCAACA GATGTCGTTG TTCCAGAGCT GATGAGGGGT ATCTCGAAGC ACACGAAACT TTTTCCTTCC TTCATTCACG CACACTACTC TCTAATGAGC AACGGTATAC GGCCTTCCTT CCAGTTACTT GAATTTGAAA TAAAAAAAAG TTTGCTGTCT TGCTATCAAG TATAAATAGA CCTGCAATTA TTAATCTTTT GTTTCCTCGT CATTGTTCTC GTTCCCTTTC TTCCTTGTTT CTTTTTCTGC ACAATATTTC AAGCTATACC AAGCATACAA TCAACTCCAA GCTTTGCAAA GATGGATAAA GCGGAATTAA TTCCCGAGCC TCCAAAAAAG AAGAGAAAGG TCGAATTGGG TACCGCCGCC AATTTTAATC AAAGTGGGAA TATTGCTGAT AGCTCATTGT CCTTCACTTT CACTAACAGT AGCAACGGTC CGAACCTCAT AACAACTCAA ACAAATTCTC AAGCGCTTTC ACAACCAATT GCCTCCTCTA ACGTTCATGA TAACTTCATG AATAATGAAA TCACGGCTAG TAAAATTGAT GATGGTAATA ATTCAAAACC ACTGTCACCT GGTTGGACGG ACCAAACTGC GTATAACGCG TTTGGAATCA CTACAGGGAT GTTTAATACC ACTACAATGG ATGATGTATA TAACTATCTA TTCGATGATG AAGATACCCC ACCAAACCCA AAAAAAGAGA TCTTTAATAC GACTCACTAT AGGGCGAGCG CCGCCATGGA GTACCCATAC GACGTACCAG ATTACGCTCA TATGGCCATG GAGGCCAGTG AATTCGGAAC TGATGAATGG GAGCAGTGGT GGAATGCCTT TAATGAGGAA AACCTGTTTT GCTCAGAAGA AATGCCATCT AGTGATGATG AGGCTACTGC TGACTCTCAA CATTCTACTC CTCCAAAAAA GAAGAGAAAG GTAGAAGACC CCAAGGACTT TCCTTCAGAA TTGCTAAGTT TTTTGAGTCA TGCTGTGTTT AGTAATAGAA CTCTTGCTTG CTTTGCTATT TACACCACAA AGGAAAAAGC TGCACTGCTA TACAAGAAAA TTATGGAAAA ATATTCTGTA ACCTTTATAA GTAGGCATAA CAGTTATAAT CATAACATAC TGTTTTTTCT TACTCCACAC AGGCATAGAG TGTCTGCTAT TAATAACTAT GCTCAAAAAT TGTGTACCTT TAGCTTTTTA ATTTGTAAAG GGGTTAATAA GGAATATTTG ATGTATAGTG CCTTGACTAG AGATCCATTT TCTGTTATTG AGGAAAGTTT GCCAGGTGGG TTAAAGGAGC ATGATTTTAA TCCAGAAGAA GCAGAGGAAA CTAAACAAGT GTCCTGGAAG CTTGTAACAG AGTATGCAAT GGAAACAAAA TGTGATGATG TGTTGTTATT GCTTGGGATG TACTTGGAAT TTCAGTACAG TTTTGAAATG TGTTTAAAAT GTATTAAAAA AGAACAGCCC AGCCACTATA AGTACCATGA AAAGCATTAT GCAAATGCTG CTATATTTGC TGACAGCAAA AACCAAAAAA CCATATGCCA ACAGGCTGTT GATACTGTTT TAGCTAAAAA GCGGGTTGAT AGCCTACAAT TAACTAGAGA ACAAATGTTA ACAAACAGAT TTAATGATCT TTTGGATAGG ATGGATATAA TGTTTGGTTC TACAGGCTCT GCTGACATAG AAGAATGGAT GGCTGGAGTT GCTTGGCTAC ACTGTTTGTT GCCCAAAATG GATTCAGTGG TGTATGACTT TTTAAAATGC ATGGTGTACA ACATTCCTAA AAAAAGATAC TGGCTGTTTA AAGGACCAAT TGATAGTGGT AAAACTACAT TAGCAGCTGC TTTGCTTGAA TTATGTGGGG GGAAAGCTTT AAATGTTAAT TTGCCCTTGG ACAGGCTGAA CTTTGAGCTA GGAGTAGCTA TTGACCAGTT TTTAGTAGTT TTTGAGGATG TAAAGGGCAC TGGAGGGGAG TCCAGAGATT TGCCTTCAGG TCAGGGAATT AATAACCTGG ACAATTTAAG GGATTATTTG GATGGCAGTG TTAAGGTAAA CTTAGAAAAG AAACACCTAA ATAAAAGAAC TCAAATATTT CCCCCTGGAA TAGTCACCAT GAATGAGTAC AGTGTGCCTA AAACACTGCA GGCCAGATTT GTAAAACAAA TAGATTTTAG GCCCAAAGAT TATTTAAAGC ATTGCCTGGA ACGCAGTGAG TTTTTGTTAG AAAAGAGAAT AATTCAAAGT GGCATTGCTT TGCTTCTTAT GTTAATTTGG TACAGACCTG TGGCTGAGTT TGCTCAAAGT ATTCAGAGCA GAATTGTGGA GTGGAAAGAG AGATTGGACA AAGAGTTTAG TTTGTCAGTG TATCAAAAAA TGAAGTTTAA TGTGGCTATG GGAATTGGAG TTTTAGATTG GCTAAGAAAC AGTGATGATG ATGATGAAGA CAGCCAGGAA AATGCTGATA AAAATGAAGA TGGTGGGGAG AAGAACATGG AAGACTCAGG GCATGAAACA GGCATTGATT CACAGTCCCA AGGCTCATTT CAGGCCCCTC AGTCCTCACA GTCTGTTCAT GATCATAATC AGCCATACCA CATTTGTAGA GGTTTTACTT GCTTTAAAAA ACCTCCCACA CCTCCCCCTG AACCTGAAAC ATAAAATGAA TGCAATTGTT GTTGTTAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTCGAG CTGCAGATGA ATCGTAGATA CTGAAAAACC CCGCAAGTTC ACTTCAACTG TGCATCGTGC ACCATCTCAA TTTCTTTCAT TTATACATCG TTTTGCCTTC TTTTATGTAA CTATACTCCT CTAAGTTTCA ATCTTGGCCA TGTAACCTCT GATCTATAGA ATTTTTTAAA TGACTAGAAT TAATGCCCAT CTTTTTTTTG GACCTAAATT CTTCATGAAA ATATATTACG AGGGCTTATT CAGAAGCTTT GGACTTCTTC GCCAGAGGTT TGGTCAAGTC TCCAATCAAG GTTGTCGGCT TGTCTACCTT GCCAGAAATT TACGAAAAGA TGGAAAAGGG TCAAATCGTT GGTAGATACG TTGTTGACAC TTCTAAATAA GCGAATTTCT TATGATTTAT GATTTTTATT ATTAAATAAG TTATAAAAAA AATAAGTGTA TACAAATTTT AAAGTGACTC TTAGGTTTTA AAACGAAAAT TCTTATTCTT GAGTAACTCT TTCCTGTAGG TCAGGTTGCT TTCTCAGGTA TAGCATGAGG TCGCTCTTAT TGACCACACC TCTACCGGCC GGTCGAAATT CCCCTACCCT ATGAACATAT TCCATTTTGT AATTTCGTGT CGTTTCTATT ATGAATTTCA TTTATAAAGT TTATGTACAA ATATCATAAA AAAAGAGAAT CTTTTTAAGC AAGGATTTTC TTAACTTCTT CGGCGACAGC ATCACCGACT TCGGTGGTAC TGTTGGAACC ACCTAAATCA CCAGTTCTGA TACCTGCATC CAAAACCTTT TTAACTGCAT CTTCAATGGC CTTACCTTCT TCAGGCAAGT TCAATGACAA TTTCAACATC ATTGCAGCAG ACAAGATAGT GGCGATAGGG TTGACCTTAT TCTTTGGCAA ATCTGGAGCA GAACCGTGGC ATGGTTCGTA CAAACCAAAT GCGGTGTTCT TGTCTGGCAA AGAGGCCAAG GACGCAGATG GCAACAAACC CAAGGAACCT GGGATAACGG AGGCTTCATC GGAGATGATA TCACCAAACA TGTTGCTGGT GATTATAATA CCATTTAGGT GGGTTGGGTT CTTAACTAGG ATCATGGCGG CAGAATCAAT CAATTGATGT TGAACCTTCA ATGTAGGAAA TTCGTTCTTG ATGGTTTCCT CCACAGTTTT TCTCCATAAT CTTGAAGAGG CCAAAACATT AGCTTTATCC AAGGACCAAA TAGGCAATGG TGGCTCATGT TGTAGGGCCA TGAAAGCGGC CATTCTTGTG ATTCTTTGCA CTTCTGGAAC GGTGTATTGT TCACTATCCC AAGCGACACC ATCACCATCG TCTTCCTTTC TCTTACCAAA GTAAATACCT CCCACTAATT CTCTGACAAC AACGAAGTCA GTACCTTTAG CAAATTGTGG CTTGATTGGA GATAAGTCTA AAAGAGAGTC GGATGCAAAG TTACATGGTC TTAAGTTGGC GTACAATTGA AGTTCTTTAC GGATTTTTAG TAAACCTTGT TCAGGTCTAA CACTACCTGT ACCCCATTTA GGACCACCCA CAGCACCTAA CAAAACGGCA TCAACCTTCT TGGAGGCTTC CAGCGCCTCA TCTGGAAGTG GGACACCTGT AGCGTCGATA GCAGCACCAC CAATTAAATG ATTTTCGAAA TCGAACTTGA CATTGGAACG AACATCAGAA ATAGCTTTAA GAACCTTAAT GGCTTCGGCT GTGATTTCTT GACCAACGTG GTCACCTGGC AAAACGACGA TCTTCTTAGG GGCAGACATT AGAATGGTAT ATCCTTGAAA TATATATATA TATTGCTGAA ATGTAAAAGG TAAGAAAAGT TAGAAAGTAA GACGATTGCT AACCACCTAT TGGAAAAAAC AATAGGTCCT TAAATAATAT TGTCAACTTC AAGTATTGTG ATGCAAGCAT TTAGTCATGA ACGCTTCTCT ATTCTATATG AAAAGCCGGT TCCGGCGCTC TCACCTTTCC TTTTTCTCCC AATTTTTCAG TTGAAAAAGG TATATGCGTC AGGCGACCTC TGAAATTAAC AAAAAATTTC CAGTCATCGA ATTTGATTCT GTGCGATAGC GCCCCTGTGT GTTCTCGTTA TGTTGAGGAA AAAAATAATG GTTGCTAAGA GATTCGAACT CTTGCATCTT ACGATACCTG AGTATTCCCA CAGTTGGGGA TCTCGACTCT AGCTAGAGGA TCAATTCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGATAAC TTCGTATAAT GTATGCTATA CGAAGTTATT AGGTCTGAAG AGGAGTTTAC GTCCAGCCAA GCTAGCTTGG CTGCAGGTCG AGCGGCCGCG ATCCGGAACC CTTAATATAA CTTCGTATAA TGTATGCTAT ACGAAGTTAT CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC CCTTTCGTCT CGCGCGTTTC GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT CGGGGCTGGC TTAACTATGC GGCATCAGAG CAGATTGTAC TGAGAGTGCA CCATAACGCA TTTAAGCATA AACACGCACT ATGCCGTTCT TCTCATGTAT ATATATATAC AGGCAACACG CAGATATAGG TGCGACGTGA ACAGTGAGCT GTATGTGCGC AGCTCGCGTT GCATTTTCGG AAGCGCTCGT TTTCGGAAAC GCTTTGAAGT TCCTATTCCG AAGTTCCTAT TCTCTAGCTA GAAAGTATAG GAACTTCAGA GCGCTTTTGA AAACCAAAAG CGCTCTGAAG ACGCACTTTC AAAAAACCAA AAACGCACCG GACTGTAACG AGCTACTAAA ATATTGCGAA TACCGCTTCC ACAAACATTG CTCAAAAGTA TCTCTTTGCT ATATATCTCT GTGCTATATC CCTATATAAC CTACCCATCC ACCTTTCGCT CCTTGAACTT GCATCTAAAC TCGACCTCTA CATTTTTTAT GTTTATCTCT AGTATTACTC TTTAGACAAA AAAATTGTAG TAAGAACTAT TCATAGAGTG AATCGAAAAC AATACGAAAA TGTAAACATT TCCTATACGT AGTATATAGA GACAAAATAG AAGAAACCGT TCATAATTTT CTGACCAATG AAGAATCATC AACGCTATCA CTTTCTGTTC ACAAAGTATG CGCAATCCAC ATCGGTATAG AATATAATCG GGGATGCCTT TATCTTGAAA AAATGCACCC GCAGCTTCGC TAGTAATCAG TAAACGCGGG AAGTGGAGTC AGGCTTTTTT TATGGAAGAG AAAATAGACA CCAAAGTAGC CTTCTTCTAA CCTTAACGGA CCTACAGTGC AAAAAGTTAT CAAGAGACTG CATTATAGAG CGCACAAAGG AGAAAAAAAG TAATCTAAGA TGCTTTGTTA GAAAAATAGC GCTCTCGGGA TGCATTTTTG TAGAACAAAA AAGAAGTATA GATTCTTTGT TGGTAAAATA GCGCTCTCGC GTTGCATTTC TGTTCTGTAA AAATGCAGCT CAGATTCTTT GTTTGAAAAA TTAGCGCTCT CGCGTTGCAT TTTTGTTTTA CAAAAATGAA GCACAGATTC TTCGTTGGTA AAATAGCGCT TTCGCGTTGC ATTTCTGTTC TGTAAAAATG CAGCTCAGAT TCTTTGTTTG AAAAATTAGC GCTCTCGCGT TGCATTTTTG TTCTACAAAA TGAAGCACAG ATGCTTCGTT GCT
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