pTRV2
$55.00
In stock
SKU
PL84847
DB00097;
100μg liquid plasmid(Instantly):$57
| name | pTRV2 | another name | apTRV2 |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Interfere with silence | Fragment type | shRNA |
| Fragment species | Empty vector | Prokaryotic resistance | Kan |
| Selective marker | Fluorescent labeling | ||
| Promoter | 35s | Replicon | pVS1 |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ATAAAACATT GCACCTATGG TGTTGCCCTG GCTGGGGTAT GTCAGTGATC GCAGTAGAAT GTACTAATTG ACAAGTTGGA GAATACGGTA GAACGTCCTT ATCCAACACA GCCTTTATCC CTCTCCCTGA CGAGGTTTTT GTCAGTGTAA TATTTCTTTT TGAACTATCC AGCTTAGTAC CGTACGGGAA AGTGACTGGT GTGCTTATCT TTGAAATGTT ACTTTGGGTT TCGGTTCTTT AGGTTAGTAA GAAAGCACTT GTCTTCTCAT ACAAAGGAAA ACCTGAGACG TATCGCTTAC GAAAGTAGCA ATGAAAGAAA GGTGGTGGTT TTAATCGCTA CCGCAAAAAC GATGGGGTCG TTTTAATTAA CTTCTCCTAC GCAAGCGTCT AAACGGACGT TGGGGTTTTG CTAGTTTCTT TAGAGAAAAC TAGCTAAGTC TTTAATGTTA TCATTAGAGA TGGCATAAAT ATAATACTTG TGTCTGCTGA TAAGATCATT TTAATTTGGA CGATTAGACT TGTTGAACTA CAGGTTACTG AATCACTTGC GCTAATCAAC ATGGGAGATA TGTACGATGA ATCATTTGAC AAGTCGGGCG GTCCTGCTGA CTTGATGGAC GATTCTTGGG TGGAATCAGT TTCGTGGAAA GATCTGTTGA AGAAGTTACA CAGCATAAAA TTTGCACTAC AGTCTGGTAG AGATGAGATC ACTGGGTTAC TAGCGGCACT GAATAGACAG TGTCCTTATT CACCATATGA GCAGTTTCCA GATAAGAAGG TGTATTTCCT TTTAGACTCA CGGGCTAACA GTGCTCTTGG TGTGATTCAG AACGCTTCAG CGTTCAAGAG ACGAGCTGAT GAGAAGAATG CAGTGGCGGG TGTTACAAAT ATTCCTGCGA ATCCAAACAC AACGGTTACG ACGAACCAAG GGAGTACTAC TACTACCAAG GCGAACACTG GCTCGACTTT GGAAGAAGAC TTGTACACTT ATTACAAATT CGATGATGCC TCTACAGCTT TCCACAAATC TCTAACTTCG TTAGAGAACA TGGAGTTGAA GAGTTATTAC CGAAGGAACT TTGAGAAAGT ATTCGGGATT AAGTTTGGTG GAGCAGCTGC TAGTTCATCT GCACCGCCTC CAGCGAGTGG AGGTCCGATA CGTCCTAATC CCTAGGGATT TAAGGACGTG AACTCTGTTG AGATCTCTGT GAAATTCAGA GGGTGGGTGA TACCATATTC ACTGATGCCA TTAGCGACAT CTAAATAGGG CTAATTGTGA CTAATTTGAG GGAATTTCCT TTACCATTGA CGTCAGTGTC GTTGGTAGCA TTTGAGTTTC GCAATGCACG AATTACTTAG GAAGTGGCTT GACGACACTA ATGTGTTATT GTTAGATAAT GGTTTGGTGG TCAAGGTACG TAGTAGAGTC CCACATATTC GCACGTATGA AGTAATTGGA AAGTTGTCAG TTTTTGATAA TTCACTGGGA GATGATACGC TGTTTGAGGG AAAAGTAGAG AACGTATTTG TTTTTATGTT CAGGCGGTTC TTGTGTGTCA ACAAAGATGG ACATTGTTAC TCAAGGAAGC ACGATGAGCT TTATTATTAC GGACGAGTGG ACTTAGATTC TGTGAGTAAG GTTACCGAAT TCTCTAGAAG GCCTCCATGG GGATCCGGTA CCGAGCTCAC GCGTCTCGAG GCCCGGGCAT GTCCCGAAGA CATTAAACTA CGGTTCTTTA AGTAGATCCG TGTCTGAAGT TTTAGGTTCA ATTTAAACCT ACGAGATTGA CATTCTCGAC TGATCTTGAT TGATCGGTAA GTCTTTTGTA ATTTAATTTT CTTTTTGATT TTATTTTAAA TTGTTATCTG TTTCTGTGTA TAGACTGTTT GAGATCGGCG TTTGGCCGAC TCATTGTCTT ACCATAGGGG AACGGACTTT GTTTGTGTTG TTATTTTATT TGTATTTTAT TAAAATTCTC AACGATCTGA AAAAGCCTCG CGGCTAAGAG ATTGTTGGGG GGTGAGTAAG TACTTTTAAA GTGATGATGG TTACAAAGGC AAAAGGGGTA AAACCCCTCG CCTACGTAAG CGTTATTACG CCCGTCTGTA CTTATATCAG TACACTGACG AGTCCCTAAA GGACGAAACG GGAGAACGCT AGCCACCACC ACCACCACCA CGTGTGAATT ACAGGTGACC AGCTCGAATT TCCCCGATCG TTCAAACATT TGGCAATAAA GTTTCTTAAG ATTGAATCCT GTTGCCGGTC TTGCGATGAT TATCATATAA TTTCTGTTGA ATTACGTTAA GCATGTAATA ATTAACATGT AATGCATGAC GTTATTTATG AGATGGGTTT TTATGATTAG AGTCCCGCAA TTATACATTT AATACGCGAT AGAAAACAAA ATATAGCGCG CAAACTAGGA TAAATTATCG CGCGCGGTGT CATCTATGTT ACTAGATCGG GAATTAAACT ATCAGTGTTT GACAGGATAT ATTGGCGGGT AAACCTAAGA GAAAAGAGCG TTTATTAGAA TAACGGATAT TTAAAAGGGC GTGAAAAGGT TTATCCGTTC GTCCATTTGT ATGTGCATGC CAACCACAGG GTTCCCCTCG GGATCAAAGT ACTTTGATCC AACCCCTCCG CTGCTATAGT GCAGTCGGCT TCTGACGTTC AGTGCAGCCG TCTTCTGAAA ACGACATGTC GCACAAGTCC TAAGTTACGC GACAGGCTGC CGCCCTGCCC TTTTCCTGGC GTTTTCTTGT CGCGTGTTTT AGTCGCATAA AGTAGAATAC TTGCGACTAG AACCGGAGAC ATTACGCCAT GAACAAGAGC GCCGCCGCTG GCCTGCTGGG CTATGCCCGC GTCAGCACCG ACGACCAGGA CTTGACCAAC CAACGGGCCG AACTGCACGC GGCCGGCTGC ACCAAGCTGT TTTCCGAGAA GATCACCGGC ACCAGGCGCG ACCGCCCGGA GCTGGCCAGG ATGCTTGACC ACCTACGCCC TGGCGACGTT GTGACAGTGA CCAGGCTAGA CCGCCTGGCC CGCAGCACCC GCGACCTACT GGACATTGCC GAGCGCATCC AGGAGGCCGG CGCGGGCCTG CGTAGCCTGG CAGAGCCGTG GGCCGACACC ACCACGCCGG CCGGCCGCAT GGTGTTGACC GTGTTCGCCG GCATTGCCGA GTTCGAGCGT TCCCTAATCA TCGACCGCAC CCGGAGCGGG CGCGAGGCCG CCAAGGCCCG AGGCGTGAAG TTTGGCCCCC GCCCTACCCT CACCCCGGCA CAGATCGCGC ACGCCCGCGA GCTGATCGAC CAGGAAGGCC GCACCGTGAA AGAGGCGGCT GCACTGCTTG GCGTGCATCG CTCGACCCTG TACCGCGCAC TTGAGCGCAG CGAGGAAGTG ACGCCCACCG AGGCCAGGCG GCGCGGTGCC TTCCGTGAGG ACGCATTGAC CGAGGCCGAC GCCCTGGCGG CCGCCGAGAA TGAACGCCAA GAGGAACAAG CATGAAACCG CACCAGGACG GCCAGGACGA ACCGTTTTTC ATTACCGAAG AGATCGAGGC GGAGATGATC GCGGCCGGGT ACGTGTTCGA GCCGCCCGCG CACGTCTCAA CCGTGCGGCT GCATGAAATC CTGGCCGGTT TGTCTGATGC CAAGCTGGCG GCCTGGCCGG CCAGCTTGGC CGCTGAAGAA ACCGAGCGCC GCCGTCTAAA AAGGTGATGT GTATTTGAGT AAAACAGCTT GCGTCATGCG GTCGCTGCGT ATATGATGCG ATGAGTAAAT AAACAAATAC GCAAGGGGAA CGCATGAAGG TTATCGCTGT ACTTAACCAG AAAGGCGGGT CAGGCAAGAC GACCATCGCA ACCCATCTAG CCCGCGCCCT GCAACTCGCC GGGGCCGATG TTCTGTTAGT CGATTCCGAT CCCCAGGGCA GTGCCCGCGA TTGGGCGGCC GTGCGGGAAG ATCAACCGCT AACCGTTGTC GGCATCGACC GCCCGACGAT TGACCGCGAC GTGAAGGCCA TCGGCCGGCG CGACTTCGTA GTGATCGACG GAGCGCCCCA GGCGGCGGAC TTGGCTGTGT CCGCGATCAA GGCAGCCGAC TTCGTGCTGA TTCCGGTGCA GCCAAGCCCT TACGACATAT GGGCCACCGC CGACCTGGTG GAGCTGGTTA AGCAGCGCAT TGAGGTCACG GATGGAAGGC TACAAGCGGC CTTTGTCGTG TCGCGGGCGA TCAAAGGCAC GCGCATCGGC GGTGAGGTTG CCGAGGCGCT GGCCGGGTAC GAGCTGCCCA TTCTTGAGTC CCGTATCACG CAGCGCGTGA GCTACCCAGG CACTGCCGCC GCCGGCACAA CCGTTCTTGA ATCAGAACCC GAGGGCGACG CTGCCCGCGA GGTCCAGGCG CTGGCCGCTG AAATTAAATC AAAACTCATT TGAGTTAATG AGGTAAAGAG AAAATGAGCA AAAGCACAAA CACGCTAAGT GCCGGCCGTC CGAGCGCACG CAGCAGCAAG GCTGCAACGT TGGCCAGCCT GGCAGACACG CCAGCCATGA AGCGGGTCAA CTTTCAGTTG CCGGCGGAGG ATCACACCAA GCTGAAGATG TACGCGGTAC GCCAAGGCAA GACCATTACC GAGCTGCTAT CTGAATACAT CGCGCAGCTA CCAGAGTAAA TGAGCAAATG AATAAATGAG TAGATGAATT TTAGCGGCTA AAGGAGGCGG CATGGAAAAT CAAGAACAAC CAGGCACCGA CGCCGTGGAA TGCCCCATGT GTGGAGGAAC GGGCGGTTGG CCAGGCGTAA GCGGCTGGGT TGTCTGCCGG CCCTGCAATG GCACTGGAAC CCCCAAGCCC GAGGAATCGG CGTGACGGTC GCAAACCATC CGGCCCGGTA CAAATCGGCG CGGCGCTGGG TGATGACCTG GTGGAGAAGT TGAAGGCCGC GCAGGCCGCC CAGCGGCAAC GCATCGAGGC AGAAGCACGC CCCGGTGAAT CGTGGCAAGC GGCCGCTGAT CGAATCCGCA AAGAATCCCG GCAACCGCCG GCAGCCGGTG CGCCGTCGAT TAGGAAGCCG CCCAAGGGCG ACGAGCAACC AGATTTTTTC GTTCCGATGC TCTATGACGT GGGCACCCGC GATAGTCGCA GCATCATGGA CGTGGCCGTT TTCCGTCTGT CGAAGCGTGA CCGACGAGCT GGCGAGGTGA TCCGCTACGA GCTTCCAGAC GGGCACGTAG AGGTTTCCGC AGGGCCGGCC GGCATGGCCA GTGTGTGGGA TTACGACCTG GTACTGATGG CGGTTTCCCA TCTAACCGAA TCCATGAACC GATACCGGGA AGGGAAGGGA GACAAGCCCG GCCGCGTGTT CCGTCCACAC GTTGCGGACG TACTCAAGTT CTGCCGGCGA GCCGATGGCG GAAAGCAGAA AGACGACCTG GTAGAAACCT GCATTCGGTT AAACACCACG CACGTTGCCA TGCAGCGTAC GAAGAAGGCC AAGAACGGCC GCCTGGTGAC GGTATCCGAG GGTGAAGCCT TGATTAGCCG CTACAAGATC GTAAAGAGCG AAACCGGGCG GCCGGAGTAC ATCGAGATCG AGCTAGCTGA TTGGATGTAC CGCGAGATCA CAGAAGGCAA GAACCCGGAC GTGCTGACGG TTCACCCCGA TTACTTTTTG ATCGATCCCG GCATCGGCCG TTTTCTCTAC CGCCTGGCAC GCCGCGCCGC AGGCAAGGCA GAAGCCAGAT GGTTGTTCAA GACGATCTAC GAACGCAGTG GCAGCGCCGG AGAGTTCAAG AAGTTCTGTT TCACCGTGCG CAAGCTGATC GGGTCAAATG ACCTGCCGGA GTACGATTTG AAGGAGGAGG CGGGGCAGGC TGGCCCGATC CTAGTCATGC GCTACCGCAA CCTGATCGAG GGCGAAGCAT CCGCCGGTTC CTAATGTACG GAGCAGATGC TAGGGCAAAT TGCCCTAGCA GGGGAAAAAG GTCGAAAAGG TCTCTTTCCT GTGGATAGCA CGTACATTGG GAACCCAAAG CCGTACATTG GGAACCGGAA CCCGTACATT GGGAACCCAA AGCCGTACAT TGGGAACCGG TCACACATGT AAGTGACTGA TATAAAAGAG AAAAAAGGCG ATTTTTCCGC CTAAAACTCT TTAAAACTTA TTAAAACTCT TAAAACCCGC CTGGCCTGTG CATAACTGTC TGGCCAGCGC ACAGCCGAAG AGCTGCAAAA AGCGCCTACC CTTCGGTCGC TGCGCTCCCT ACGCCCCGCC GCTTCGCGTC GGCCTATCGC GGCCGCTGGC CGCTCAAAAA TGGCTGGCCT ACGGCCAGGC AATCTACCAG GGCGCGGACA AGCCGCGCCG TCGCCACTCG ACCGCCGGCG CCCACATCAA GGCACCCTGC CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCGC AGCCATGACC CAGTCACGTA GCGATAGCGG AGTGTATACT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGCAT TCTAGGTACT AAAACAATTC ATCCAGTAAA ATATAATATT TTATTTTCTC CCAATCAGGC TTGATCCCCA GTAAGTCAAA AAATAGCTCG ACATACTGTT CTTCCCCGAT ATCCTCCCTG ATCGACCGGA CGCAGAAGGC AATGTCATAC CACTTGTCCG CCCTGCCGCT TCTCCCAAGA TCAATAAAGC CACTTACTTT GCCATCTTTC ACAAAGATGT TGCTGTCTCC CAGGTCGCCG TGGGAAAAGA CAAGTTCCTC TTCGGGCTTT TCCGTCTTTA AAAAATCATA CAGCTCGCGC GGATCTTTAA ATGGAGTGTC TTCTTCCCAG TTTTCGCAAT CCACATCGGC CAGATCGTTA TTCAGTAAGT AATCCAATTC GGCTAAGCGG CTGTCTAAGC TATTCGTATA GGGACAATCC GATATGTCGA TGGAGTGAAA GAGCCTGATG CACTCCGCAT ACAGCTCGAT AATCTTTTCA GGGCTTTGTT CATCTTCATA CTCTTCCGAG CAAAGGACGC CATCGGCCTC ACTCATGAGC AGATTGCTCC AGCCATCATG CCGTTCAAAG TGCAGGACCT TTGGAACAGG CAGCTTTCCT TCCAGCCATA GCATCATGTC CTTTTCCCGT TCCACATCAT AGGTGGTCCC TTTATACCGG CTGTCCGTCA TTTTTAAATA TAGGTTTTCA TTTTCTCCCA CCAGCTTATA TACCTTAGCA GGAGACATTC CTTCCGTATC TTTTACGCAG CGGTATTTTT CGATCAGTTT TTTCAATTCC GGTGATATTC TCATTTTAGC CATTTATTAT TTCCTTCCTC TTTTCTACAG TATTTAAAGA TACCCCAAGA AGCTAATTAT AACAAGACGA ACTCCAATTC ACTGTTCCTT GCATTCTAAA ACCTTAAATA CCAGAAAACA GCTTTTTCAA AGTTGTTTTC AAAGTTGGCG TATAACATAG TATCGACGGA GCCGATTTTG AAACCGCGGT GATCACAGGC AGCAACGCTC TGTCATCGTT ACAATCAACA TGCTACCCTC CGCGAGATCA TCCGTGTTTC AAACCCGGCA GCTTAGTTGC CGTTCTTCCG AATAGCATCG GTAACATGAG CAAAGTCTGC CGCCTTACAA CGGCTCTCCC GCTGACGCCG TCCCGGACTG ATGGGCTGCC TGTATCGAGT GGTGATTTTG TGCCGAGCTG CCGGTCGGGG AGCTGTTGGC TGGCTGGTGG CAGGATATAT TGTGGTGTAA ACAAATTGAC GCTTAGACAA CTTAATAACA CATTGCGGAC GTTTTTAATG TACTGAATTA ACGCCGAATT AATTCCTAGG CCACCATGTT GGGCCCGGCG CGCCAAGCTT GCATGCCTGC AGGTCAACAT GGTGGAGCAC GACACTCTCG TCTACTCCAA GAATATCAAA GATACAGTCT CAGAAGACCA GAGGGCTATT GAGACTTTTC AACAAAGGGT AATATCGGGA AACCTCCTCG GATTCCATTG CCCAGCTATC TGTCACTTCA TCGAAAGGAC AGTAGAAAAG GAAGATGGCT TCTACAAATG CCATCATTGC GATAAAGGAA AGGCTATCGT TCAAGATGCC TCTACCGACA GTGGTCCCAA AGATGGACCC CCACCCACGA GGAACATCGT GGAAAAAGAA GACGTTCCAA CCACGTCTTC AAAGCAAGTG GATTGATGTG ATGGTCAACA TGGTGGAGCA CGACACTCTC GTCTACTCCA AGAATATCAA AGATACAGTC TCAGAAGACC AGAGGGCTAT TGAGACTTTT CAACAAAGGG TAATATCGGG AAACCTCCTC GGATTCCATT GCCCAGCTAT CTGTCACTTC ATCGAAAGGA CAGTAGAAAA GGAAGATGGC TTCTACAAAT GCCATCATTG CGATAAAGGA AAGGCTATCG TTCAAGATGC CTCTACCGAC AGTGGTCCCA AAGATGGACC CCCACCCACG AGGAACATCG TGGAAAAAGA AGACGTTCCA ACCACGTCTT CAAAGCAAGT GGATTGATGT GATATCTCCA CTGACGTAAG GGATGACGCA CAATCCCACT ATCCTTCGCA AGACCCTTCC TCTATATAAG GAAGTTCATT TCATTTGGAG AGG
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