pPIC9K-His
$55.00
In stock
SKU
PL84836
DB00066;
100μg liquid plasmid(Instantly):$57
| name | pPIC9K-His | another name | apPIC9K-His |
| Insert Size (bp) | Plasmid host | Yeast bacterium | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Empty vector | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418,HIS4 | Fluorescent labeling | |
| Promoter | AOX1 | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | Forward left ITR | |
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AGATCTAACA TCCAAAGACG AAAGGTTGAA TGAAACCTTT TTGCCATCCG ACATCCACAG GTCCATTCTC ACACATAAGT GCCAAACGCA ACAGGAGGGG ATACACTAGC AGCAGACCGT TGCAAACGCA GGACCTCCAC TCCTCTTCTC CTCAACACCC ACTTTTGCCA TCGAAAAACC AGCCCAGTTA TTGGGCTTGA TTGGAGCTCG CTCATTCCAA TTCCTTCTAT TAGGCTACTA ACACCATGAC TTTATTAGCC TGTCTATCCT GGCCCCCCTG GCGAGGTTCA TGTTTGTTTA TTTCCGAATG CAACAAGCTC CGCATTACAC CCGAACATCA CTCCAGATGA GGGCTTTCTG AGTGTGGGGT CAAATAGTTT CATGTTCCCC AAATGGCCCA AAACTGACAG TTTAAACGCT GTCTTGGAAC CTAATATGAC AAAAGCGTGA TCTCATCCAA GATGAACTAA GTTTGGTTCG TTGAAATGCT AACGGCCAGT TGGTCAAAAA GAAACTTCCA AAAGTCGCCA TACCGTTTGT CTTGTTTGGT ATTGATTGAC GAATGCTCAA AAATAATCTC ATTAATGCTT AGCGCAGTCT CTCTATCGCT TCTGAACCCC GGTGCACCTG TGCCGAAACG CAAATGGGGA AACACCCGCT TTTTGGATGA TTATGCATTG TCTCCACATT GTATGCTTCC AAGATTCTGG TGGGAATACT GCTGATAGCC TAACGTTCAT GATCAAAATT TAACTGTTCT AACCCCTACT TGACAGCAAT ATATAAACAG AAGGAAGCTG CCCTGTCTTA AACCTTTTTT TTTATCATCA TTATTAGCTT ACTTTCATAA TTGCGACTGG TTCCAATTGA CAAGCTTTTG ATTTTAACGA CTTTTAACGA CAACTTGAGA AGATCAAAAA ACAACTAATT ATTCGAAGGA TCCAAACGAT GAGATTTCCT TCAATTTTTA CTGCAGTTTT ATTCGCAGCA TCCTCCGCAT TAGCTGCTCC AGTCAACACT ACAACAGAAG ATGAAACGGC ACAAATTCCG GCTGAAGCTG TCATCGGTTA CTCAGATTTA GAAGGGGATT TCGATGTTGC TGTTTTGCCA TTTTCCAACA GCACAAATAA CGGGTTATTG TTTATAAATA CTACTATTGC CAGCATTGCT GCTAAAGAAG AAGGGGTATC TCTCGAGAAA AGAGAGGCTG AAGCTTACGT AGAATTCCCT AGGGCGGCCG CGAATCATCA TCACCATCAC CATTAATTCG CCTTAGACAT GACTGTTCCT CAGTTCAAGT TGGGCACTTA CGAGAAGACC GGTCTTGCTA GATTCTAATC AAGAGGATGT CAGAATGCCA TTTGCCTGAG AGATGCAGGC TTCATTTTTG ATACTTTTTT ATTTGTAACC TATATAGTAT AGGATTTTTT TTGTCATTTT GTTTCTTCTC GTACGAGCTT GCTCCTGATC AGCCTATCTC GCAGCTGATG AATATCTTGT GGTAGGGGTT TGGGAAAATC ATTCGAGTTT GATGTTTTTC TTGGTATTTC CCACTCCTCT TCAGAGTACA GAAGATTAAG TGAGAAGTTC GTTTGTGCAA GCTTATCGAT AAGCTTTAAT GCGGTAGTTT ATCACAGTTA AATTGCTAAC GCAGTCAGGC ACCGTGTATG AAATCTAACA ATGCGCTCAT CGTCATCCTC GGCACCGTCA CCCTGGATGC TGTAGGCATA GGCTTGGTTA TGCCGGTACT GCCGGGCCTC TTGCGGGATA TCGTCCATTC CGACAGCATC GCCAGTCACT ATGGCGTGCT GCTAGCGCTA TATGCGTTGA TGCAATTTCT ATGCGCACCC GTTCTCGGAG CACTGTCCGA CCGCTTTGGC CGCCGCCCAG TCCTGCTCGC TTCGCTACTT GGAGCCACTA TCGACTACGC GATCATGGCG ACCACACCCG TCCTGTGGAT CTATCGAATC TAAATGTAAG TTAAAATCTC TAAATAATTA AATAAGTCCC AGTTTCTCCA TACGAACCTT AACAGCATTG CGGTGAGCAT CTAGACCTTC AACAGCAGCC AGATCCATCA CTGCTTGGCC AATATGTTTC AGTCCCTCAG GAGTTACGTC TTGTGAAGTG ATGAACTTCT GGAAGGTTGC AGTGTTAACT CCGCTGTATT GACGGGCATA TCCGTACGTT GGCAAAGTGT GGTTGGTACC GGAGGAGTAA TCTCCACAAC TCTCTGGAGA GTAGGCACCA ACAAACACAG ATCCAGCGTG TTGTACTTGA TCAACATAAG AAGAAGCATT CTCGATTTGC AGGATCAAGT GTTCAGGAGC GTACTGATTG GACATTTCCA AAGCCTGCTC GTAGGTTGCA ACCGATAGGG TTGTAGAGTG TGCAATACAC TTGCGTACAA TTTCAACCCT TGGCAACTGC ACAGCTTGGT TGTGAACAGC ATCTTCAATT CTGGCAAGCT CCTTGTCTGT CATATCGACA GCCAACAGAA TCACCTGGGA ATCAATACCA TGTTCAGCTT GAGACAGAAG GTCTGAGGCA ACGAAATCTG GATCAGCGTA TTTATCAGCA ATAACTAGAA CTTCAGAAGG CCCAGCAGGC ATGTCAATAC TACACAGGGC TGATGTGTCA TTTTGAACCA TCATCTTGGC AGCAGTAACG AACTGGTTTC CTGGACCAAA TATTTTGTCA CACTTAGGAA CAGTTTCTGT TCCGTAAGCC ATAGCAGCTA CTGCCTGGGC GCCTCCTGCT AGCACGATAC ACTTAGCACC AACCTTGTGG GCAACGTAGA TGACTTCTGG GGTAAGGGTA CCATCCTTCT TAGGTGGAGA TGCAAAAACA ATTTCTTTGC AACCAGCAAC TTTGGCAGGA ACACCCAGCA TCAGGGAAGT GGAAGGCAGA ATTGCGGTTC CACCAGGAAT ATAGAGGCCA ACTTTCTCAA TAGGTCTTGC AAAACGAGAG CAGACTACAC CAGGGCAAGT CTCAACTTGC AACGTCTCCG TTAGTTGAGC TTCATGGAAT TTCCTGACGT TATCTATAGA GAGATCAATG GCTCTCTTAA CGTTATCTGG CAATTGCATA AGTTCCTCTG GGAAAGGAGC TTCTAACACA GGTGTCTTCA AAGCGACTCC ATCAAACTTG GCAGTTAGTT CTAAAAGGGC TTTGTCACCA TTTTGACGAA CATTGTCGAC AATTGGTTTG ACTAATTCCA TAATCTGTTC CGTTTTCTGG ATAGGACGAC GAAGGGCATC TTCAATTTCT TGTGAGGAGG CCTTAGAAAC GTCAATTTTG CACAATTCAA TACGACCTTC AGAAGGGACT TCTTTAGGTT TGGATTCTTC TTTAGGTTGT TCCTTGGTGT ATCCTGGCTT GGCATCTCCT TTCCTTCTAG TGACCTTTAG GGACTTCATA TCCAGGTTTC TCTCCACCTC GTCCAACGTC ACACCGTACT TGGCACATCT AACTAATGCA AAATAAAATA AGTCAGCACA TTCCCAGGCT ATATCTTCCT TGGATTTAGC TTCTGCAAGT TCATCAGCTT CCTCCCTAAT TTTAGCGTTC AACAAAACTT CGTCGTCAAA TAACCGTTTG GTATAAGAAC CTTCTGGAGC ATTGCTCTTA CGATCCCACA AGGTGGCTTC CATGGCTCTA AGACCCTTTG ATTGGCCAAA ACAGGAAGTG CGTTCCAAGT GACAGAAACC AACACCTGTT TGTTCAACCA CAAATTTCAA GCAGTCTCCA TCACAATCCA ATTCGATACC CAGCAACTTT TGAGTTGCTC CAGATGTAGC ACCTTTATAC CACAAACCGT GACGACGAGA TTGGTAGACT CCAGTTTGTG TCCTTATAGC CTCCGGAATA GACTTTTTGG ACGAGTACAC CAGGCCCAAC GAGTAATTAG AAGAGTCAGC CACCAAAGTA GTGAATAGAC CATCGGGGCG GTCAGTAGTC AAAGACGCCA ACAAAATTTC ACTGACAGGG AACTTTTTGA CATCTTCAGA AAGTTCGTAT TCAGTAGTCA ATTGCCGAGC ATCAATAATG GGGATTATAC CAGAAGCAAC AGTGGAAGTC ACATCTACCA ACTTTGCGGT CTCAGAAAAA GCATAAACAG TTCTACTACC GCCATTAGTG AAACTTTTCA AATCGCCCAG TGGAGAAGAA AAAGGCACAG CGATACTAGC ATTAGCGGGC AAGGATGCAA CTTTATCAAC CAGGGTCCTA TAGATAACCC TAGCGCCTGG GATCATCCTT TGGACAACTC TTTCTGCCAA ATCTAGGTCC AAAATCACTT CATTGATACC ATTATTGTAC AACTTGAGCA AGTTGTCGAT CAGCTCCTCA AATTGGTCCT CTGTAACGGA TGACTCAACT TGCACATTAA CTTGAAGCTC AGTCGATTGA GTGAACTTGA TCAGGTTGTG CAGCTGGTCA GCAGCATAGG GAAACACGGC TTTTCCTACC AAACTCAAGG AATTATCAAA CTCTGCAACA CTTGCGTATG CAGGTAGCAA GGGAAATGTC ATACTTGAAG TCGGACAGTG AGTGTAGTCT TGAGAAATTC TGAAGCCGTA TTTTTATTAT CAGTGAGTCA GTCATCAGGA GATCCTCTAC GCCGGACGCA TCGTGGCCGA CCTGCAGGGG GGGGGGGGGC GCTGAGGTCT GCCTCGTGAA GAAGGTGTTG CTGACTCATA CCAGGCCTGA ATCGCCCCAT CATCCAGCCA GAAAGTGAGG GAGCCACGGT TGATGAGAGC TTTGTTGTAG GTGGACCAGT TGGTGATTTT GAACTTTTGC TTTGCCACGG AACGGTCTGC GTTGTCGGGA AGATGCGTGA TCTGATCCTT CAACTCAGCA AAAGTTCGAT TTATTCAACA AAGCCGCCGT CCCGTCAAGT CAGCGTAATG CTCTGCCAGT GTTACAACCA ATTAACCAAT TCTGATTAGA AAAACTCATC GAGCATCAAA TGAAACTGCA ATTTATTCAT ATCAGGATTA TCAATACCAT ATTTTTGAAA AAGCCGTTTC TGTAATGAAG GAGAAAACTC ACCGAGGCAG TTCCATAGGA TGGCAAGATC CTGGTATCGG TCTGCGATTC CGACTCGTCC AACATCAATA CAACCTATTA ATTTCCCCTC GTCAAAAATA AGGTTATCAA GTGAGAAATC ACCATGAGTG ACGACTGAAT CCGGTGAGAA TGGCAAAAGC TTATGCATTT CTTTCCAGAC TTGTTCAACA GGCCAGCCAT TACGCTCGTC ATCAAAATCA CTCGCATCAA CCAAACCGTT ATTCATTCGT GATTGCGCCT GAGCGAGACG AAATACGCGA TCGCTGTTAA AAGGACAATT ACAAACAGGA ATCGAATGCA ACCGGCGCAG GAACACTGCC AGCGCATCAA CAATATTTTC ACCTGAATCA GGATATTCTT CTAATACCTG GAATGCTGTT TTCCCGGGGA TCGCAGTGGT GAGTAACCAT GCATCATCAG GAGTACGGAT AAAATGCTTG ATGGTCGGAA GAGGCATAAA TTCCGTCAGC CAGTTTAGTC TGACCATCTC ATCTGTAACA TCATTGGCAA CGCTACCTTT GCCATGTTTC AGAAACAACT CTGGCGCATC GGGCTTCCCA TACAATCGAT AGATTGTCGC ACCTGATTGC CCGACATTAT CGCGAGCCCA TTTATACCCA TATAAATCAG CATCCATGTT GGAATTTAAT CGCGGCCTCG AGCAAGACGT TTCCCGTTGA ATATGGCTCA TAACACCCCT TGTATTACTG TTTATGTAAG CAGACAGTTT TATTGTTCAT GATGATATAT TTTTATCTTG TGCAATGTAA CATCAGAGAT TTTGAGACAC AACGTGGCTT TCCCCCCCCC CCCTGCAGGT CGGCATCACC GGCGCCACAG GTGCGGTTGC TGGCGCCTAT ATCGCCGACA TCACCGATGG GGAAGATCGG GCTCGCCACT TCGGGCTCAT GAGCGCTTGT TTCGGCGTGG GTATGGTGGC AGGCCCCGTG GCCGGGGGAC TGTTGGGCGC CATCTCCTTG CATGCACCAT TCCTTGCGGC GGCGGTGCTC AACGGCCTCA ACCTACTACT GGGCTGCTTC CTAATGCAGG AGTCGCATAA GGGAGAGCGT CGAGTATCTA TGATTGGAAG TATGGGAATG GTGATACCCG CATTCTTCAG TGTCTTGAGG TCTCCTATCA GATTATGCCC AACTAAAGCA ACCGGAGGAG GAGATTTCAT GGTAAATTTC TCTGACTTTT GGTCATCAGT AGACTCGAAC TGTGAGACTA TCTCGGTTAT GACAGCAGAA ATGTCCTTCT TGGAGACAGT AAATGAAGTC CCACCAATAA AGAAATCCTT GTTATCAGGA ACAAACTTCT TGTTTCGAAC TTTTTCGGTG CCTTGAACTA TAAAATGTAG AGTGGATATG TCGGGTAGGA ATGGAGCGGG CAAATGCTTA CCTTCTGGAC CTTCAAGAGG TATGTAGGGT TTGTAGATAC TGATGCCAAC TTCAGTGACA ACGTTGCTAT TTCGTTCAAA CCATTCCGAA TCCAGAGAAA TCAAAGTTGT TTGTCTACTA TTGATCCAAG CCAGTGCGGT CTTGAAACTG ACAATAGTGT GCTCGTGTTT TGAGGTCATC TTTGTATGAA TAAATCTAGT CTTTGATCTA AATAATCTTG ACGAGCCAAG GCGATAAATA CCCAAATCTA AAACTCTTTT AAAACGTTAA AAGGACAAGT ATGTCTGCCT GTATTAAACC CCAAATCAGC TCGTAGTCTG ATCCTCATCA ACTTGAGGGG CACTATCTTG TTTTAGAGAA ATTTGCGGAG ATGCGATATC GAGAAAAAGG TACGCTGATT TTAAACGTGA AATTTATCTC AAGATCTCTG CCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG TGTTGGCGGG TGTCGGGGCG CAGCCATGAC CCAGTCACGT AGCGATAGCG GAGTGTATAC TGGCTTAACT ATGCGGCATC AGAGCAGATT GTACTGAGAG TGCACCATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAA TGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTG CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA CACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGTC TAAGAAACCA TTATTATCAT GACATTAACC TATAAAAATA GGCGTATCAC GAGGCCCTTT CGTCTTCAAG AATTAATTCT CATGTTTGAC AGCTTATCAT CGATAAGCTG ACTCATGTTG GTATTGTGAA ATAGACGCAG ATCGGGAACA CTGAAAAATA ACAGTTATTA TTCG
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