pAAV-DJ
$55.00
In stock
SKU
PL84826
DB00069;
100μg liquid plasmid(Instantly):$57
| name | pAAV-DJ | another name | apAAV-DJ |
| Insert Size (bp) | Plasmid host | Mammary cell,Adenovirus | |
| Plasmid uses | packaging aid | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pUC | |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CCGCCATGCC GGGGTTTTAC GAGATTGTGA TTAAGGTCCC CAGCGACCTT GACGAGCATC TGCCCGGCAT TTCTGACAGC TTTGTGAACT GGGTGGCCGA GAAGGAATGG GAGTTGCCGC CAGATTCTGA CATGGATCTG AATCTGATTG AGCAGGCACC CCTGACCGTG GCCGAGAAGC TGCAGCGCGA CTTTCTGACG GAATGGCGCC GTGTGAGTAA GGCCCCGGAG GCTCTTTTCT TTGTGCAATT TGAGAAGGGA GAGAGCTACT TCCACATGCA CGTGCTCGTG GAAACCACCG GGGTGAAATC CATGGTTTTG GGACGTTTCC TGAGTCAGAT TCGCGAAAAA CTGATTCAGA GAATTTACCG CGGGATCGAG CCGACTTTGC CAAACTGGTT CGCGGTCACA AAGACCAGAA ATGGCGCCGG AGGCGGGAAC AAGGTGGTGG ATGAGTGCTA CATCCCCAAT TACTTGCTCC CCAAAACCCA GCCTGAGCTC CAGTGGGCGT GGACTAATAT GGAACAGTAT TTAAGCGCCT GTTTGAATCT CACGGAGCGT AAACGGTTGG TGGCGCAGCA TCTGACGCAC GTGTCGCAGA CGCAGGAGCA GAACAAAGAG AATCAGAATC CCAATTCTGA TGCGCCGGTG ATCAGATCAA AAACTTCAGC CAGGTACATG GAGCTGGTCG GGTGGCTCGT GGACAAGGGG ATTACCTCGG AGAAGCAGTG GATCCAGGAG GACCAGGCCT CATACATCTC CTTCAATGCG GCCTCCAACT CGCGGTCCCA AATCAAGGCT GCCTTGGACA ATGCGGGAAA GATTATGAGC CTGACTAAAA CCGCCCCCGA CTACCTGGTG GGCCAGCAGC CCGTGGAGGA CATTTCCAGC AATCGGATTT ATAAAATTTT GGAACTAAAC GGGTACGATC CCCAATATGC GGCTTCCGTC TTTCTGGGAT GGGCCACGAA AAAGTTCGGC AAGAGGAACA CCATCTGGCT GTTTGGGCCT GCAACTACCG GGAAGACCAA CATCGCGGAG GCCATAGCCC ACACTGTGCC CTTCTACGGG TGCGTAAACT GGACCAATGA GAACTTTCCC TTCAACGACT GTGTCGACAA GATGGTGATC TGGTGGGAGG AGGGGAAGAT GACCGCCAAG GTCGTGGAGT CGGCCAAAGC CATTCTCGGA GGAAGCAAGG TGCGCGTGGA CCAGAAATGC AAGTCCTCGG CCCAGATAGA CCCGACTCCC GTGATCGTCA CCTCCAACAC CAACATGTGC GCCGTGATTG ACGGGAACTC AACGACCTTC GAACACCAGC AGCCGTTGCA AGACCGGATG TTCAAATTTG AACTCACCCG CCGTCTGGAT CATGACTTTG GGAAGGTCAC CAAGCAGGAA GTCAAAGACT TTTTCCGGTG GGCAAAGGAT CACGTGGTTG AGGTGGAGCA TGAATTCTAC GTCAAAAAGG GTGGAGCCAA GAAAAGACCC GCCCCCAGTG ACGCAGATAT AAGTGAGCCC AAACGGGTGC GCGAGTCAGT TGCGCAGCCA TCGACGTCAG ACGCGGAAGC TTCGATCAAC TACGCAGACA GGTACCAAAA CAAATGTTCT CGTCACGTGG GCATGAATCT GATGCTGTTT CCCTGCAGAC AATGCGAGAG AATGAATCAG AATTCAAATA TCTGCTTCAC TCACGGACAG AAAGACTGTT TAGAGTGCTT TCCCGTGTCA GAATCTCAAC CCGTTTCTGT CGTCAAAAAG GCGTATCAGA AACTGTGCTA CATTCATCAT ATCATGGGAA AGGTGCCAGA CGCTTGCACT GCCTGCGATC TGGTCAATGT GGATTTGGAT GACTGCATCT TTGAACAATA AATGATTTAA ATCAGGTATG GCTGCCGATG GTTATCTTCC AGATTGGCTC GAGGACACTC TCTCTGAAGG AATAAGACAG TGGTGGAAGC TCAAACCTGG CCCACCACCA CCAAAGCCCG CAGAGCGGCA TAAGGACGAC AGCAGGGGTC TTGTGCTTCC TGGGTACAAG TACCTCGGAC CCTTCAACGG ACTCGACAAG GGAGAGCCGG TCAACGAGGC AGACGCCGCG GCCCTCGAGC ACGACAAAGC CTACGACCGG CAGCTCGACA GCGGAGACAA CCCGTACCTC AAGTACAACC ACGCCGACGC CGAGTTCCAG GAGCGGCTCA AAGAAGATAC GTCTTTTGGG GGCAACCTCG GGCGAGCAGT CTTCCAGGCC AAAAAGAGGC TTCTTGAACC TCTTGGTCTG GTTGAGGAAG CGGCTAAGAC GGCTCCTGGA AAGAAGAGGC CTGTAGAGCA CTCTCCTGTG GAGCCAGACT CCTCCTCGGG AACCGGAAAG GCGGGCCAGC AGCCTGCAAG AAAAAGATTG AATTTTGGTC AGACTGGAGA CGCAGACTCA GTCCCAGACC CTCAACCAAT CGGAGAACCT CCCGCAGCCC CCTCAGGTGT GGGATCTCTT ACAATGGCTG CAGGCGGTGG CGCACCAATG GCAGACAATA ACGAGGGCGC CGACGGAGTG GGTAATTCCT CGGGAAATTG GCATTGCGAT TCCACATGGA TGGGCGACAG AGTCATCACC ACCAGCACCC GAACCTGGGC CCTGCCCACC TACAACAACC ACCTCTACAA GCAAATCTCC AACAGCACAT CTGGAGGATC TTCAAATGAC AACGCCTACT TCGGCTACAG CACCCCCTGG GGGTATTTTG ACTTTAACAG ATTCCACTGC CACTTTTCAC CACGTGACTG GCAGCGACTC ATCAACAACA ACTGGGGATT CCGGCCCAAG AGACTCAGCT TCAAGCTCTT CAACATCCAG GTCAAGGAGG TCACGCAGAA TGAAGGCACC AAGACCATCG CCAATAACCT CACCAGCACC ATCCAGGTGT TTACGGACTC GGAGTACCAG CTGCCGTACG TTCTCGGCTC TGCCCACCAG GGCTGCCTGC CTCCGTTCCC GGCGGACGTG TTCATGATTC CCCAGTACGG CTACCTAACA CTCAACAACG GTAGTCAGGC CGTGGGACGC TCCTCCTTCT ACTGCCTGGA ATACTTTCCT TCGCAGATGC TGAGAACCGG CAACAACTTC CAGTTTACTT ACACCTTCGA GGACGTGCCT TTCCACAGCA GCTACGCCCA CAGCCAGAGC TTGGACCGGC TGATGAATCC TCTGATTGAC CAGTACCTGT ACTACTTGTC TCGGACTCAA ACAACAGGAG GCACGACAAA TACGCAGACT CTGGGCTTCA GCCAAGGTGG GCCTAATACA ATGGCCAATC AGGCAAAGAA CTGGCTGCCA GGACCCTGTT ACCGCCAGCA GCGAGTATCA AAGACATCTG CGGATAACAA CAACAGTGAA TACTCGTGGA CTGGAGCTAC CAAGTACCAC CTCAATGGCA GAGACTCTCT GGTGAATCCG GGCCCGGCCA TGGCAAGCCA CAAGGACGAT GAAGAAAAGT TTTTTCCTCA GAGCGGGGTT CTCATCTTTG GGAAGCAAGG CTCAGAGAAA ACAAATGTGG ACATTGAAAA GGTCATGATT ACAGACGAAG AGGAAATCAG GACAACCAAT CCCGTGGCTA CGGAGCAGTA TGGTTCTGTA TCTACCAACC TCCAGAGAGG CAACAGACAA GCAGCTACCG CAGATGTCAA CACACAAGGC GTTCTTCCAG GCATGGTCTG GCAGGACAGA GATGTGTACC TTCAGGGGCC CATCTGGGCA AAGATTCCAC ACACGGACGG ACATTTTCAC CCCTCTCCCC TCATGGGTGG ATTCGGACTT AAACACCCTC CGCCTCAGAT CCTGATCAAG AACACGCCTG TACCTGCGGA TCCTCCGACC ACCTTCAACC AGTCAAAGCT GAACTCTTTC ATCACCCAGT ATTCTACTGG CCAAGTCAGC GTGGAGATCG AGTGGGAGCT GCAGAAGGAA AACAGCAAGC GCTGGAACCC CGAGATCCAG TACACCTCCA ACTACTACAA ATCTACAAGT GTGGACTTTG CTGTTAATAC AGAAGGCGTG TACTCTGAAC CCCGCCCCAT TGGCACCCGT TACCTCACCC GTAATCTGTA ATTGCCTGTT AATCAATAAA CCGGTTGATT CGTTTCAGTT GAACTTTGGT CTCTGCGAAG GGCGAATTCG TTTAAACCTG CAGGACTAGA GGTCCTGTAT TAGAGGTCAC GTGAGTGTTT TGCGACATTT TGCGACACCA TGTGGTCACG CTGGGTATTT AAGCCCGAGT GAGCACGCAG GGTCTCCATT TTGAAGCGGG AGGTTTGAAC GCGCAGCCGC CAAGCCGAAT TCTGCAGATA TCCATCACAC TGGCGGCCGC TCGACTAGAG CGGCCGCCAC CGCGGTGGAG CTCCAGCTTT TGTTCCCTTT AGTGAGGGTT AATTGCGCGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTAAAT TGTAAGCGTT AATATTTTGT TAAAATTCGC GTTAAATTTT TGTTAAATCA GCTCATTTTT TAACCAATAG GCCGAAATCG GCAAAATCCC TTATAAATCA AAAGAATAGA CCGAGATAGG GTTGAGTGTT GTTCCAGTTT GGAACAAGAG TCCACTATTA AAGAACGTGG ACTCCAACGT CAAAGGGCGA AAAACCGTCT ATCAGGGCGA TGGCCCACTA CGTGAACCAT CACCCTAATC AAGTTTTTTG GGGTCGAGGT GCCGTAAAGC ACTAAATCGG AACCCTAAAG GGAGCCCCCG ATTTAGAGCT TGACGGGGAA AGCCGGCGAA CGTGGCGAGA AAGGAAGGGA AGAAAGCGAA AGGAGCGGGC GCTAGGGCGC TGGCAAGTGT AGCGGTCACG CTGCGCGTAA CCACCACACC CGCCGCGCTT AATGCGCCGC TACAGGGCGC GTCCCATTCG CCATTCAGGC TGCGCAACTG TTGGGAAGGG CGATCGGTGC GGGCCTCTTC GCTATTACGC CAGCTGGCGA AAGGGGGATG TGCTGCAAGG CGATTAAGTT GGGTAACGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC GACGGCCAGT GAGCGCGCGT AATACGACTC ACTATAGGGC GAATTGGGTA CCGGGCCCCC CCTCGATCGA GGTCGACGGT ATCGGGGGAG CTCGCAGGGT CTCCATTTTG AAGCGGGAGG TTTGAACGCG CAG
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