pAAV-RC6
$55.00
In stock
SKU
PL84824
DB00075;pAAV-6;pAAV-6
100μg liquid plasmid(Instantly):$57
| name | pAAV-RC6 | another name | apAAV-RC6 |
| Insert Size (bp) | Plasmid host | Mammary cell,Adenovirus | |
| Plasmid uses | packaging aid | Fragment type | |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pUC | |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ATCGTTAACG CCCCGCGCCG GCCGCTCTAG AACTAGTGGA TCCCCCGGAA GATCAGAAGT TCCTATTCCG AAGTTCCTAT TCTCTAGAAA GTATAGGAAC TTCTGATCTG CGCAGCCGCC ATGCCGGGGT TTTACGAGAT TGTGATTAAG GTCCCCAGCG ACCTTGACGA GCATCTGCCC GGCATTTCTG ACAGCTTTGT GAACTGGGTG GCCGAGAAGG AATGGGAGTT GCCGCCAGAT TCTGACATGG ATCTGAATCT GATTGAGCAG GCACCCCTGA CCGTGGCCGA GAAGCTGCAG CGCGACTTTC TGACGGAATG GCGCCGTGTG AGTAAGGCCC CGGAGGCCCT TTTCTTTGTG CAATTTGAGA AGGGAGAGAG CTACTTCCAC ATGCACGTGC TCGTGGAAAC CACCGGGGTG AAATCCATGG TTTTGGGACG TTTCCTGAGT CAGATTCGCG AAAAACTGAT TCAGAGAATT TACCGCGGGA TCGAGCCGAC TTTGCCAAAC TGGTTCGCGG TCACAAAGAC CAGAAATGGC GCCGGAGGCG GGAACAAGGT GGTGGATGAG TGCTACATCC CCAATTACTT GCTCCCCAAA ACCCAGCCTG AGCTCCAGTG GGCGTGGACT AATATGGAAC AGTATTTAAG CGCCTGTTTG AATCTCACGG AGCGTAAACG GTTGGTGGCG CAGCATCTGA CGCACGTGTC GCAGACGCAG GAGCAGAACA AAGAGAATCA GAATCCCAAT TCTGATGCGC CGGTGATCAG ATCAAAAACT TCAGCCAGGT ACATGGAGCT GGTCGGGTGG CTCGTGGACA AGGGGATTAC CTCGGAGAAG CAGTGGATCC AGGAGGACCA GGCCTCATAC ATCTCCTTCA ATGCGGCCTC CAACTCGCGG TCCCAAATCA AGGCTGCCTT GGACAATGCG GGAAAGATTA TGAGCCTGAC TAAAACCGCC CCCGACTACC TGGTGGGCCA GCAGCCCGTG GAGGACATTT CCAGCAATCG GATTTATAAA ATTTTGGAAC TAAACGGGTA CGATCCCCAA TATGCGGCTT CCGTCTTTCT GGGATGGGCC ACGAAAAAGT TCGGCAAGAG GAACACCATC TGGCTGTTTG GGCCTGCAAC TACCGGGAAG ACCAACATCG CGGAGGCCAT AGCCCACACT GTGCCCTTCT ACGGGTGCGT AAACTGGACC AATGAGAACT TTCCCTTCAA CGACTGTGTC GACAAGATGG TGATCTGGTG GGAGGAGGGG AAGATGACCG CCAAGGTCGT GGAGTCGGCC AAAGCCATTC TCGGAGGAAG CAAGGTGCGC GTGGACCAGA AATGCAAGTC CTCGGCCCAG ATAGACCCGA CTCCCGTGAT CGTCACCTCC AACACCAACA TGTGCGCCGT GATTGACGGG AACTCAACGA CCTTCGAACA CCAGCAGCCG TTGCAAGACC GGATGTTCAA ATTTGAACTC ACCCGCCGTC TGGATCATGA CTTTGGGAAG GTCACCAAGC AGGAAGTCAA AGACTTTTTC CGGTGGGCAA AGGATCACGT GGTTGAGGTG GAGCATGAAT TCTACGTCAA AAAGGGTGGA GCCAAGAAAA GACCCGCCCC CAGTGACGCA GATATAAGTG AGCCCAAACG GGTGCGCGAG TCAGTTGCGC AGCCATCGAC GTCAGACGCG GAAGCTTCGA TCAACTACGC AGACAGGTAC CAAAACAAAT GTTCTCGTCA CGTGGGCATG AATCTGATGC TGTTTCCCTG CAGACAATGC GAGAGAATGA ATCAGAATTC AAATATCTGC TTCACTCACG GACAGAAAGA CTGTTTAGAG TGCTTTCCCG TGTCAGAATC TCAACCCGTT TCTGTCGTCA AAAAGGCGTA TCAGAAACTG TGCTACATTC ATCATATCAT GGGAAAGGTG CCAGACGCTT GCACTGCCTG CGATCTGGTC AATGTGGATT TGGATGACTG CATCTTTGAA CAATAAATGA TTTAAATCAG GTATGGCTGC CGATGGTTAT CTTCCAGATT GGCTCGAGGA CAACCTCTCT GAGGGCATTC GCGAGTGGTG GGACTTGAAA CCTGGAGCCC CGAAACCCAA AGCCAACCAG CAAAAGCAGG ACGACGGCCG GGGTCTGGTG CTTCCTGGCT ACAAGTACCT CGGACCCTTC AACGGACTCG ACAAGGGGGA GCCCGTCAAC GCGGCGGATG CAGCGGCCCT CGAGCACGAC AAGGCCTACG ACCAGCAGCT CAAAGCGGGT GACAATCCGT ACCTGCGGTA TAACCACGCC GACGCCGAGT TTCAGGAGCG TCTGCAAGAA GATACGTCTT TTGGGGGCAA CCTCGGGCGA GCAGTCTTCC AGGCCAAGAA GAGGGTTCTC GAACCTCTTG GTCTGGTTGA GGAAGGTGCT AAGACGGCTC CTGGAAAGAA ACGTCCGGTA GAGCAGTCGC CACAAGAGCC AGACTCCTCC TCGGGCATTG GCAAGACAGG CCAGCAGCCC GCTAAAAAGA GACTCAATTT TGGTCAGACT GGCGACTCAG AGTCAGTCCC CGACCCACAA CCTCTCGGAG AACCTCCAGC AACCCCCGCT GCTGTGGGAC CTACTACAAT GGCTTCAGGC GGTGGCGCAC CAATGGCAGA CAATAACGAA GGCGCCGACG GAGTGGGTAA TGCCTCAGGA AATTGGCATT GCGATTCCAC ATGGCTGGGC GACAGAGTCA TCACCACCAG CACCCGAACA TGGGCCTTGC CCACCTATAA CAACCACCTC TACAAGCAAA TCTCCAGTGC TTCAACGGGG GCCAGCAACG ACAACCACTA CTTCGGCTAC AGCACCCCCT GGGGGTATTT TGATTTCAAC AGATTCCACT GCCATTTCTC ACCACGTGAC TGGCAGCGAC TCATCAACAA CAATTGGGGA TTCCGGCCCA AGAGACTCAA CTTCAAGCTC TTCAACATCC AAGTCAAGGA GGTCACGACG AATGATGGCG TCACGACCAT CGCTAATAAC CTTACCAGCA CGGTTCAAGT CTTCTCGGAC TCGGAGTACC AGTTGCCGTA CGTCCTCGGC TCTGCGCACC AGGGCTGCCT CCCTCCGTTC CCGGCGGACG TGTTCATGAT TCCGCAGTAC GGCTACCTAA CGCTCAACAA TGGCAGCCAG GCAGTGGGAC GGTCATCCTT TTACTGCCTG GAATATTTCC CATCGCAGAT GCTGAGAACG GGCAATAACT TTACCTTCAG CTACACCTTC GAGGACGTGC CTTTCCACAG CAGCTACGCG CACAGCCAGA GCCTGGACCG GCTGATGAAT CCTCTCATCG ACCAGTACCT GTATTACCTG AACAGAACTC AGAATCAGTC CGGAAGTGCC CAAAACAAGG ACTTGCTGTT TAGCCGGGGG TCTCCAGCTG GCATGTCTGT TCAGCCCAAA AACTGGCTAC CTGGACCCTG TTACCGGCAG CAGCGCGTTT CTAAAACAAA AACAGACAAC AACAACAGCA ACTTTACCTG GACTGGTGCT TCAAAATATA ACCTTAATGG GCGTGAATCT ATAATCAACC CTGGCACTGC TATGGCCTCA CACAAAGACG ACAAAGACAA GTTCTTTCCC ATGAGCGGTG TCATGATTTT TGGAAAGGAG AGCGCCGGAG CTTCAAACAC TGCATTGGAC AATGTCATGA TCACAGACGA AGAGGAAATC AAAGCCACTA ACCCCGTGGC CACCGAAAGA TTTGGGACTG TGGCAGTCAA TCTCCAGAGC AGCAGCACAG ACCCTGCGAC CGGAGATGTG CATGTTATGG GAGCCTTACC TGGAATGGTG TGGCAAGACA GAGACGTATA CCTGCAGGGT CCTATTTGGG CCAAAATTCC TCACACGGAT GGACACTTTC ACCCGTCTCC TCTCATGGGC GGCTTTGGAC TTAAGCACCC GCCTCCTCAG ATCCTCATCA AAAACACGCC TGTTCCTGCG AATCCTCCGG CAGAGTTTTC GGCTACAAAG TTTGCTTCAT TCATCACCCA GTATTCCACA GGACAAGTGA GCGTGGAGAT TGAATGGGAG CTGCAGAAAG AAAACAGCAA ACGCTGGAAT CCCGAAGTGC AGTATACATC TAACTATGCA AAATCTGCCA ACGTTGATTT CACTGTGGAC AACAATGGAC TTTATACTGA GCCTCGCCCC ATTGGCACCC GTTACCTCAC CCGTCCCCTG TAATTGTGTG TTAATCAATA AACCGGTTAA TTCGTGTCAG TTGAACTTTG GTCTCATGTC GTTATTATCT TATCTGGTCA CCAGATCCCC GTAGATAAGT AGCATGGCGG GTTAATCATT AACTACAGCC CGGGCGTTTA AACAGCGGGC GGAGGGGTGG AGTCGTGACG TGAATTACGT CATAGGGTTA GGGAGGTCCT GTATTAGAGG TCACGTGAGT GTTTTGCGAC ATTTTGCGAC ACCATGTGGT CTCGCTGGGG GGGGGGGCCC GAGTGAGCAC GCAGGGTCTC CATTTTGAAG CGGGAGGTTT GAACGAGCGC TGGCGCGCTC ACTGGCCGTC GTTTTACAAC GTCGTGACTG GGAAAACCCT GGCGTTACCC AACTTAATCG CCTTGCAGCA CATCCCCCTT TCGCCAGCTG GCGTAATAGC GAAGAGGCCC GCACCGATCG CCCTTCCCAA CAGTTGCGCA GCCTGAATGG CGAATGGAAA TTGTAAGCGT TAATATTTTG TTAAAATTCG CGTTAAATTT TTGTTAAATC AGCTCATTTT TTTAACCAAT AGGCCGAAAT CGGCAAAATC CCTTATAAAT CAAAAGAATA GACCGAGATA GGGTTGAGTG TTGTTCCAGT TTGGAACAAG AGTCCACTAT TAAGAACGTG GACTCCAACG TCAAAGGGCG AAAAACCGTC TATCAGGGCG ATGGCCCACT ACGTGAACCA TCACCCTAAT CAAGTTTTTT GGGGTCGAGG TGCCGTAAAG CACTAAATCG GAACCCTAAA GGGAGCCCCC GATTTAGAGC TTGACGGGGA AAGCCGGCGA ACGTGGCGAG AAAGGAAGGG AAGAAAGCGA AAGGAGCGGG CGCTAGGGCG CTGGCAAGTG TAGCGGTCAC GCTGCGCGTA ACCACCACAC CCGCCGCGCT TAATGCGCCG CTACAGGGCG CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTATT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGTA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTTCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC TATGACCATG ATTACGCCAA GCGCGCCGAT
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
