GWIZ-GFP
$128.00
In stock
SKU
PL84801
超强荧光
100μg liquid plasmid(Instantly):$149
| name | GWIZ-GFP | another name | aGWIZ-GFP |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | Fluorescent labeling | ||
| Promoter | CMV | Replicon | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GGGGGCCTTT AGGGGGCGGA GCTATGGAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA AATTCTATGA TTTCTCTCAT TACTTCCTCA CATGTTGGAG GCATTTTCTC TCCCTCTGCA CTTAATAGCC TATCTTGCTT TAATTTCTTC CCACTCTTGG AGGCTAGGTT TGGTTTGGTG GGCTGATGAG GGAGGGAGAG ACCGCTCCAA GTACTTTAGC GGGTGGGATT GAAGGCGGAG CCCTCCTGAG CTATGAGTGT CCTATGAGTG GGGCTGGAAC TAAGAACCAG GGGCGTGGAC AGGGTGTGTC ACAGAGAAGG GGATGTGCCT GCTTCTTTCT GGCCCAGGAG GAACCGGGTC AATTCTTCAG CACCTGGGTA CCAGAGAGAG AGAGAGAGAG AGAGAGAGAG AGAGAGAGAG GTACCAGAGA GAGAGAGAGA GAGAGAGAGA GAGAGAGAGA GAGAGAGGTA CCCATAGAGC CCACCGCATC CCCAGCATGC CTGCTATTGT CTTCCCAATC CTCCCCCTTG CTGTCCTGCC CCACCCCACC CCCCAGAATA GAATGACACC TACTCAGACA ATGCGATGCA ATTTCCTCAT TTTATTAGGA AAGGACAGTG GGAGTGGCAC CTTCCAGGGT CAAGGAAGGC ACGGGGGAGG GGCAAACAAC AGATGGCTGG CAACTAGAAG GCACAGCAGA TCCACAAAAA ACCAACACAC AGATCTCTAG AGCTCTGATC TTTTATTAGC CAGAAGTGAT CTGGATCCTC AGTTGTACAG TTCATCCATG CCATGTGTAA TCCCAGCAGC TGTTACAAAC TCAAGAAGGA CCATGTGGTC TCTCTTTTCG TTGGGATCTT TCGAAAGGGC AGATTGTGTG GACAGGTAAT GGTTGTCTGG TAAAAGGACA GGGCCATCGC CAATTGGAGT ATTTTGTTGA TAATGGTCTG CTAGTTGAAC GCTTCCATCT TCAATGTTGT GGCGGGTCTT GAAGTTCACT TTGATTCCAT TCTTTTGTTT GTCTGCCATG ATGTATACAT TGTGTGAGTT ATAGTTGTAT TCCAATTTGT GTCCCAGAAT GTTGCCATCT TCCTTGAAGT CAATACCTTT TAACTCGATT CTATTAACAA GGGTATCACC TTCAAACTTG ACTTCAGCAC GTGTCTTGTA GTTGCCGTCA TCTTTGAAGA AGATGGTCCT TTCCTGTACA TAACCTTCGG GCATGGCACT CTTGAAAAAG TCATGCCGTT TCATATGATC CGGGTATCTT GAAAAGCATT GAACACCATA GCACAGAGTA GTGACTAGTG TTGGCCATGG AACAGGCAGT TTGCCAGTAG TGCAGATGAA CTTCAGGGTA AGTTTTCCGT ATGTTGCATC ACCTTCACCC TCTCCACTGA CAGAGAACTT GTGGCCGTTA ACATCACCAT CTAATTCAAC AAGAATTGGG ACAACTCCAG TGAAGAGTTC TTCTCCTTTG CTAGCCATTT CTTGCGCGCC CGTGTCGACG ACGGTGACTG CAGAAAAGAC CCATGGAAAG GAACAGTCTG TTAGTCTGTC AGCTATTATG TCTGGTGGCG CGCGCGGCAG CAACGAGTAC TGCTCAGACT ACACTGCCCT CCACCGTTAA CAGCACCGCA ACGGGAGTTA CCTCTGACTC TTATCAGAAT ACAACAACTC AGCTGCCTGC ATCTTCTTCT GCCGCTGCCT TAAGTCTTCC ATCTGCGTCA GCCGTGCGAG CCCAATCTCC ACGCTCATTT TCAGACACAT ACCCTACCGC CACGGCCTTG TGCGGCACAC TGGTGGTGGT GGGCATTGTG CTGTGCCTAA GTCTGGCCTC CACTGTTAGG AGCAAGGAGC TGCCGAGCGA CCATGAGCCG CTGGAGGCAT GGGACCAGGG CTCGGATGTG GAAGCTCCGC CGCTACCGGA GAAGAGCCCA TGTCCGGAAC ACGTACCCGA GATTCGCGTG GAGATCCCAC GCTATGTTTA ATAAAAACTG CGGGCACGGG GGACGGCGTT GTTGTATATG TGAATTTGTA AATAATAAAT GGGACCCCAT CCTGTAAAAA TACAGAGTCC GTGTCAGTCT CTGAAGGACA GAGTATTGGC ATATAGCCAA TAGAGATAGT TGTGGCAAAG AGCCATGTTA TGGATTAGTA ATGGAAAGTA TCGTCACCAA TAGGGGAGTG GTCAATAATG GTCAATAACC CACACCTATA GGCTAAGCTA TACCATCACC TATAGCATAA GGAAGCGGGG GTGTATAGGC CCCAAGCCAA AAACAGTATA GCATGCATAA GAGCCAAAGG GGTGTGCCTA TAGAGTCTAT AGGCGGTACT TACGTCACTC TTGGCACGGG GAATCCGCGT TCCAATGCAC CGTTCCCGGC CGCGGAGGCT GGATCGGTCC CGGTGTCTTC TATGGAGGTC AAAACAGCGT GGATGGCGTC TCCAGGCGAT CTGACGGTTC ACTAAACGAG CTCTGCTTAT ATAGACCTCC CACCGTACAC GCCTACCGCC CATTTGCGTC AATGGGGCGG AGTTGTTACG ACATTTTGGA AAGTCCCGTT GATTTTGGTG CCAAAACAAA CTCCCATTGA CGTCAATGGG GTGGAGACTT GGAAATCCCC GTGAGTCAAA CCGCTATCCA CGCCCATTGA TGTACTGCCA AAACCGCATC ACCATGGTAA TAGCGATGAC TAATACGTAG ATGTACTGCC AAGTAGGAAA GTCCCATAAG GTCATGTACT GGGCATAATG CCAGGCGGGC CATTTACCGT CATTGACGTC AATAGGGGGC GTACTTGGCA TATGATACAC TTGATGTACT GCCAAGTGGG CAGTTTACCG TAAATACTCC ACCCATTGAC GTCAATGGAA AGTCCCTATT GGCGTTACTA TGGGAACATA CGTCATTATT GACGTCAATG GGCGGGGGTC GTTGGGCGGT CAGCCAGGCG GGCCATTTAC CGTAAGTTAT GTAACGCGGA ACTCCATATA TGGGCTATGA ACTAATGACC CCGTAATTGA TTACTATTAA TAACTAGTCA ATAATCAATG TCAACATGGC GGTAATGTTG GACATGAGCC AATATAAATG TACATATTAT GATATGGATA CAACGTATGC AATGGCCAAT AGCCAATCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATGGGGG GGGGGGGAAA GCCACGTTGT GTCTCAAAAT CTCTGATGTT ACATTGCACA AGATAAAAAT ATATCATCAT GAACAATAAA ACTGTCTGCT TACATAAACA GTAATACAAG GGGTGTTATG AGCCATATTC AACGGGAAAC GTCTTGCTCG AGGCCGCGAT TAAATTCCAA CATGGATGCT GATTTATATG GGTATAAATG GGCTCGCGAT AATGTCGGGC AATCAGGTGC GACAATCTAT CGATTGTATG GGAAGCCCGA TGCGCCAGAG TTGTTTCTGA AACATGGCAA AGGTAGCGTT GCCAATGATG TTACAGATGA GATGGTCAGA CTAAACTGGC TGACGGAATT TATGCCTCTT CCGACCATCA AGCATTTTAT CCGTACTCCT GATGATGCAT GGTTACTCAC CACTGCGATC CCCGGGAAAA CAGCATTCCA GGTATTAGAA GAATATCCTG ATTCAGGTGA AAATATTGTT GATGCGCTGG CAGTGTTCCT GCGCCGGTTG CATTCGATTC CTGTTTGTAA TTGTCCTTTT AACAGCGATC GCGTATTTCG TCTCGCTCAG GCGCAATCAC GAATGAATAA CGGTTTGGTT GATGCGAGTG ATTTTGATGA CGAGCGTAAT GGCTGGCCTG TTGAACAAGT CTGGAAAGAA ATGCATAAGC TTTTGCCATT CTCACCGGAT TCAGTCGTCA CTCATGGTGA TTTCTCACTT GATAACCTTA TTTTTGACGA GGGGAAATTA ATAGGTTGTA TTGATGTTGG ACGAGTCGGA ATCGCAGACC GATACCAGGA TCTTGCCATC CTATGGAACT GCCTCGGTGA GTTTTCTCCT TCATTACAGA AACGGCTTTT TCAAAAATAT GGTATTGATA ATCCTGATAT GAATAAATTG CAGTTTCATT TGATGCTCGA TGAGTTTTTC TAATCAGAAT TGGTTAATTG GTTGTAACAC TGGCAGAGCA TTACGCTGAC TTGACGGGAC GGCGGCTTTG TTGAATAAAT CGAACTTTTG CTGAGTTGAA GGATCAGATC ACGCATCTTC CCGACAACGC AGACCGTTCC GTGGCAAAGC AAAAGTTCAA AATCACCAAC TGGTCCACCT ACAACAAAGC TCTCATCAAC CGTGGCTCCC TCACTTTCTG GCTGGATGAT GGGGCGATTC AGGCCTGGTA TGAGTCAGCA ACACCTTCTT CACGAGGCAG ACCTCAGCGC CCCCCCCCCC CGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTCC TGCGTATCAC CTGATGCTGT ATAACCC
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