pBinGFP2
$128.00
In stock
SKU
PL84797
100μg liquid plasmid(Instantly):$163
| name | pBinGFP2 | another name | apBinGFP2 |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | 35s | Replicon | OriV |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | 35s： | |
| 3′ sequencing primer | Copy number | low | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CCGGGCTGGT TGCCCTCGCC GCTGGGCTGG CGGCCGTCTA TGGCCCTGCA AACGCGCCAG AAACGCCGTC GAAGCCGTGT GCGAGACACC GCGGCCGCCG GCGTTGTGGA TACCTCGCGG AAAACTTGGC CCTCACTGAC AGATGAGGGG CGGACGTTGA CACTTGAGGG GCCGACTCAC CCGGCGCGGC GTTGACAGAT GAGGGGCAGG CTCGATTTCG GCCGGCGACG TGGAGCTGGC CAGCCTCGCA AATCGGCGAA AACGCCTGAT TTTACGCGAG TTTCCCACAG ATGATGTGGA CAAGCCTGGG GATAAGTGCC CTGCGGTATT GACACTTGAG GGGCGCGACT ACTGACAGAT GAGGGGCGCG ATCCTTGACA CTTGAGGGGC AGAGTGCTGA CAGATGAGGG GCGCACCTAT TGACATTTGA GGGGCTGTCC ACAGGCAGAA AATCCAGCAT TTGCAAGGGT TTCCGCCCGT TTTTCGGCCA CCGCTAACCT GTCTTTTAAC CTGCTTTTAA ACCAATATTT ATAAACCTTG TTTTTAACCA GGGCTGCGCC CTGTGCGCGT GACCGCGCAC GCCGAAGGGG GGTGCCCCCC CTTCTCGAAC CCTCCCGGCC CGCTAACGCG GGCCTCCCAT CCCCCCAGGG GCTGCGCCCC TCGGCCGCGA ACGGCCTCAC CCCAAAAATG GCAGCGCTGG CAGTCCTTGC CATTGCCGGG ATCGGGGCAG TAACGGGATG GGCGATCAGC CCGAGCGCGA CGCCCGGAAG CATTGACGTG CCGCAGGTGC TGGCATCGAC ATTCAGCGAC CAGGTGCCGG GCAGTGAGGG CGGCGGCCTG GGTGGCGGCC TGCCCTTCAC TTCGGCCGTC GGGGCATTCA CGGACTTCAT GGCGGGGCCG GCAATTTTTA CCTTGGGCAT TCTTGGCATA GTGGTCGCGG GTGCCGTGCT CGTGTTCGGG GGTGCGATAA ACCCAGCGAA CCATTTGAGG TGATAGGTAA GATTATACCG AGGTATGAAA ACGAGAATTG GACCTTTACA GAATTACTCT ATGAAGCGCC ATATTTAAAA AGCTACCAAG ACGAAGAGGA TGAAGAGGAT GAGGAGGCAG ATTGCCTTGA ATATATTGAC AATACTGATA AGATAATATA TCTTTTATAT AGAAGATATC GCCGTATGTA AGGATTTCAG GGGGCAAGGC ATAGGCAGCG CGCTTATCAA TATATCTATA GAATGGGCAA AGCATAAAAA CTTGCATGGA CTAATGCTTG AAACCCAGGA CAATAACCTT ATAGCTTGTA AATTCTATCA TAATTGGGTA ATGACTCCAA CTTATTGATA GTGTTTTATG TTCAGATAAT GCCCGATGAC TTTGTCATGC AGCTCCACCG ATTTTGAGAA CGACAGCGAC TTCCGTCCCA GCCGTGCCAG GTGCTGCCTC AGATTCAGGT TATGCCGCTC AATTCGCTGC GTATATCGCT TGCTGATTAC GTGCAGCTTT CCCTTCAGGC GGGATTCATA CAGCGGCCAG CCATCCGTCA TCCATATCAC CACGTCAAAG GGTGACAGCA GGCTCATAAG ACGCCCCAGC GTCGCCATAG TGCGTTCACC GAATACGTGC GCAACAACCG TCTTCCGGAG ACTGTCATAC GCGTAAAACA GCCAGCGCTG GCGCGATTTA GCCCCGACAT AGCCCCACTG TTCGTCCATT TCCGCGCAGA CGATGACGTC ACTGCCCGGC TGTATGCGCG AGGTTACCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATAGTT ACCGACTGCG GCCTGAGTTT TTTAAGTGAC GTAAAATCGT GTTGAGGCCA ACGCCCATAA TGCGGGCTGT TGCCCGGCAT CCAACGCCAT TCATGGCCAT ATCAATGATT TTCTGGTGCG TACCGGGTTG AGAAGCGGTG TAAGTGAACT GCAGTTGCCA TGTTTTACGG CAGTGAGAGC AGAGATAGCG CTGATGTCCG GCGGTGCTTT TGCCGTTACG CACCACCCCG TCAGTAGCTG AACAGGAGGG ACAGCTGATA GACACAGAAG CCACTGGAGC ACCTCAAAAA CACCATCATA CACTAAATCA GTAAGTTGGC AGCATCACCC ATAATTGTGG TTTCAAAATC GGCTCCGTCG ATACTATGTT ATACGCCAAC TTTGAAAACA ACTTTGAAAA AGCTGTTTTC TGGTATTTAA GGTTTTAGAA TGCAAGGAAC AGTGAATTGG AGTTCGTCTT GTTATAATTA GCTTCTTGGG GTATCTTTAA ATACTGTAGA AAAGAGGAAG GAAATAATAA ATGGCTAAAA TGAGAATATC ACCGGAATTG AAAAAACTGA TCGAAAAATA CCGCTGCGTA AAAGATACGG AAGGAATGTC TCCTGCTAAG GTATATAAGC TGGTGGGAGA AAATGAAAAC CTATATTTAA AAATGACGGA CAGCCGGTAT AAAGGGACCA CCTATGATGT GGAACGGGAA AAGGACATGA TGCTATGGCT GGAAGGAAAG CTGCCTGTTC CAAAGGTCCT GCACTTTGAA CGGCATGATG GCTGGAGCAA TCTGCTCATG AGTGAGGCCG ATGGCGTCCT TTGCTCGGAA GAGTATGAAG ATGAACAAAG CCCTGAAAAG ATTATCGAGC TGTATGCGGA GTGCATCAGG CTCTTTCACT CCATCGACAT ATCGGATTGT CCCTATACGA ATAGCTTAGA CAGCCGCTTA GCCGAATTGG ATTACTTACT GAATAACGAT CTGGCCGATG TGGATTGCGA AAACTGGGAA GAAGACACTC CATTTAAAGA TCCGCGCGAG CTGTATGATT TTTTAAAGAC GGAAAAGCCC GAAGAGGAAC TTGTCTTTTC CCACGGCGAC CTGGGAGACA GCAACATCTT TGTGAAAGAT GGCAAAGTAA GTGGCTTTAT TGATCTTGGG AGAAGCGGCA GGGCGGACAA GTGGTATGAC ATTGCCTTCT GCGTCCGGTC GATCAGGGAG GATATCGGGG AAGAACAGTA TGTCGAGCTA TTTTTTGACT TACTGGGGAT CAAGCCTGAT TGGGAGAAAA TAAAATATTA TATTTTACTG GATGAATTGT TTTAGTACCT AGATGTGGCG CAACGATGCC GGCGACAAGC AGGAGCGCAC CGACTTCTTC CGCATCAAGT GTTTTGGCTC TCAGGCCGAG GCCCACGGCA AGTATTTGGG CAAGGGGTCG CTGGTATTCG TGCAGGGCAA GATTCGGAAT ACCAAGTACG AGAAGGACGG CCAGACGGTC TACGGGACCG ACTTCATTGC CGATAAGGTG GATTATCTGG ACACCAAGGC ACCAGGCGGG TCAAATCAGG AATAAGGGCA CATTGCCCCG GCGTGAGTCG GGGCAATCCC GCAAGGAGGG TGAATGAATC GGACGTTTGA CCGGAAGGCA TACAGGCAAG AACTGATCGA CGCGGGGTTT TCCGCCGAGG ATGCCGAAAC CATCGCAAGC CGCACCGTCA TGCGTGCGCC CCGCGAAACC TTCCAGTCCG TCGGCTCGAT GGTCCAGCAA GCTACGGCCA AGATCGAGCG CGACAGCGTG CAACTGGCTC CCCCTGCCCT GCCCGCGCCA TCGGCCGCCG TGGAGCGTTC GCGTCGTCTC GAACAGGAGG CGGCAGGTTT GGCGAAGTCG ATGACCATCG ACACGCGAGG AACTATGACG ACCAAGAAGC GAAAAACCGC CGGCGAGGAC CTGGCAAAAC AGGTCAGCGA GGCCAAGCAG GCCGCGTTGC TGAAACACAC GAAGCAGCAG ATCAAGGAAA TGCAGCTTTC CTTGTTCGAT ATTGCGCCGT GGCCGGACAC GATGCGAGCG ATGCCAAACG ACACGGCCCG CTCTGCCCTG TTCACCACGC GCAACAAGAA AATCCCGCGC GAGGCGCTGC AAAACAAGGT CATTTTCCAC GTCAACAAGG ACGTGAAGAT CACCTACACC GGCGTCGAGC TGCGGGCCGA CGATGACGAA CTGGTGTGGC AGCAGGTGTT GGAGTACGCG AAGCGCACCC CTATCGGCGA GCCGATCACC TTCACGTTCT ACGAGCTTTG CCAGGACCTG GGCTGGTCGA TCAATGGCCG GTATTACACG AAGGCCGAGG AATGCCTGTC GCGCCTACAG GCGACGGCGA TGGGCTTCAC GTCCGACCGC GTTGGGCACC TGGAATCGGT GTCGCTGCTG CACCGCTTCC GCGTCCTGGA CCGTGGCAAG AAAACGTCCC GTTGCCAGGT CCTGATCGAC GAGGAAATCG TCGTGCTGTT TGCTGGCGAC CACTACACGA AATTCATATG GGAGAAGTAC CGCAAGCTGT CGCCGACGGC CCGACGGATG TTCGACTATT TCAGCTCGCA CCGGGAGCCG TACCCGCTCA AGCTGGAAAC CTTCCGCCTC ATGTGCGGAT CGGATTCCAC CCGCGTGAAG AAGTGGCGCG AGCAGGTCGG CGAAGCCTGC GAAGAGTTGC GAGGCAGCGG CCTGGTGGAA CACGCCTGGG TCAATGATGA CCTGGTGCAT TGCAAACGCT AGGGCCTTGT GGGGTCAGTT CCGGCTGGGG GTTCAGCAGC CAGCGCTTTA CTGGCATTTC AGGAACAAGC GGGCACTGCT CGACGCACTT GCTTCGCTCA GTATCGCTCG GGACGCACGG CGCGCTCTAC GAACTGCCGA TAAACAGAGG ATTAAAATTG ACAATTGTGA TTAAGGCTCA GATTCGACGG CTTGGAGCGG CCGACGTGCA GGATTTCCGC GAGATCCGAT TGTCGGCCCT GAAGAAAGCT CCAGAGATGT TCGGGTCCGT TTACGAGCAC GAGGAGAAAA AGCCCATGGA GGCGTTCGCT GAACGGTTGC GAGATGCCGT GGCATTCGGC GCCTACATCG ACGGCGAGAT CATTGGGCTG TCGGTCTTCA AACAGGAGGA CGGCCCCAAG GACGCTCACA AGGCGCATCT GTCCGGCGTT TTCGTGGAGC CCGAACAGCG AGGCCGAGGG GTCGCCGGTA TGCTGCTGCG GGCGTTGCCG GCGGGTTTAT TGCTCGTGAT GATCGTCCGA CAGATTCCAA CGGGAATCTG GTGGATGCGC ATCTTCATCC TCGGCGCACT TAATATTTCG CTATTCTGGA GCTTGTTGTT TATTTCGGTC TACCGCCTGC CGGGCGGGGT CGCGGCGACG GTAGGCGCTG TGCAGCCGCT GATGGTCGTG TTCATCTCTG CCGCTCTGCT AGGTAGCCCG ATACGATTGA TGGCGGTCCT GGGGGCTATT TGCGGAACTG CGGGCGTGGC GCTGTTGGTG TTGACACCAA ACGCAGCGCT AGATCCTGTC GGCGTCGCAG CGGGCCTGGC GGGGGCGGTT TCCATGGCGT TCGGAACCGT GCTGACCCGC AAGTGGCAAC CTCCCGTGCC TCTGCTCACC TTTACCGCCT GGCAACTGGC GGCCGGAGGA CTTCTGCTCG TTCCAGTAGC TTTAGTGTTT GATCCGCCAA TCCCGATGCC TACAGGAACC AATGTTCTCG GCCTGGCGTG GCTCGGCCTG ATCGGAGCGG GTTTAACCTA CTTCCTTTGG TTCCGGGGGA TCTCGCGACT CGAACCTACA GTTGTTTCCT TACTGGGCTT TCTCAGCCCC AGATCTGGGG TCGATCAGCC GGGGATGCAT CAGGCCGACA GTCGGAACTT CGGGTCCCCG ACCTGTACCA TTCGGTGAGC AATGGATAGG GGAGTTGATA TCGTCAACGT TCACTTCTAA AGAAATAGCG CCACTCAGCT TCCTCAGCGG CTTTATCCAG CGATTTCCTA TTATGTCGGC ATAGTTCTCA AGATCGACAG CCTGTCACGG TTAAGCGAGA AATGAATAAG AAGGCTGATA ATTCGGATCT CTGCGAGGGA GATGATATTT GATCACAGGC AGCAACGCTC TGTCATCGTT ACAATCAACA TGCTACCCTC CGCGAGATCA TCCGTGTTTC AAACCCGGCA GCTTAGTTGC CGTTCTTCCG AATAGCATCG GTAACATGAG CAAAGTCTGC CGCCTTACAA CGGCTCTCCC GCTGACGCCG TCCCGGACTG ATGGGCTGCC TGTATCGAGT GGTGATTTTG TGCCGAGCTG CCGGTCGGGG AGCTGTTGGC TGGCTGGTGG CAGGATATAT TGTGGTGTAA ACAAATTGAC GCTTAGACAA CTTAATAACA CATTGCGGAC GTTTTTAATG TACTGGGGTG GTTTTTCTTT TCACCAGTGA GACGGGCAAC AGCTGATTGC CCTTCACCGC CTGGCCCTGA GAGAGTTGCA GCAAGCGGTC CACGCTGGTT TGCCCCAGCA GGCGAAAATC CTGTTTGATG GTGGTTCCGA AATCGGCAAA ATCCCTTATA AATCAAAAGA ATAGCCCGAG ATAGGGTTGA GTGTTGTTCC AGTTTGGAAC AAGAGTCCAC TATTAAAGAA CGTGGACTCC AACGTCAAAG GGCGAAAAAC CGTCTATCAG GGCGATGGCC CACAAACTGA AGGCGGGAAA CGACAATCTG ATCATGAGCG GAGAATTAAG GGAGTCACGT TATGACCCCC GCCGATGACG CGGGACAAGC CGTTTTACGT TTGGAACTGA CAGAACCGCA ACGTTGAAGG AGCCACTCAG CCGCGGGTTT CTGGAGTTTA ATGAGCTAAG CACATACGTC AGAAACCATT ATTGCGCGTT CAAAAGTCGC CTAAGGTCAC TATCAGCTAG CAAATATTTC TTGTCAAAAA TGCTCCACTG ACGTTCCATA AATTCCCCTC GGTATCCAAT TAGAGTCTCA TATTCACTCT CAATCCAAAT AATCTGCACC GGATCTGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGATGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACGGGATCT CTGCGGAACA GGCGGTCGAA GGTGCCGATA TCATTACGAC AGCAACGGCC GACAAGCACA ACGCCACGAT CCTGAGCGAC AATATGATCG GGCCCGGCGT CCACATCAAC GGCGTCGGCG GCGACTGCCC AGGCAAGACC GAGATGCACC GCGATATCTT GCTGCGTTCG GATATTTTCG TGGAGTTCCC GCCACAGACC CGGATGATCC CCGATCGTTC AAACATTTGG CAATAAAGTT TCTTAAGATT GAATCCTGTT GCCGGTCTTG CGATGATTAT CATATAATTT CTGTTGAATT ACGTTAAGCA TGTAATAATT AACATGTAAT GCATGACGTT ATTTATGAGA TGGGTTTTTA TGATTAGAGT CCCGCAATTA TACATTTAAT ACGCGATAGA AAACAAAATA TAGCGCGCAA ACTAGGATAA ATTATCGCGC GCGGTGTCAT CTATGTTACT AGATCGGGCC TCCTGTCAAT GCTGGCGGCG GCTCTGGTGG TGGTTCTGGT GGCGGCTCTG AGGGTGGTGG CTCTGAGGGT GGCGGTTCTG AGGGTGGCGG CTCTGAGGGA GGCGGTTCCG GTGGTGGCTC TGGTTCCGGT GATTTTGATT ATGAAAAGAT GGCAAACGCT AATAAGGGGG CTATGACCGA AAATGCCGAT GAAAACGCGC TACAGTCTGA CGCTAAAGGC AAACTTGATT CTGTCGCTAC TGATTACGGT GCTGCTATCG ATGGTTTCAT TGGTGACGTT TCCGGCCTTG CTAATGGTAA TGGTGCTACT GGTGATTTTG CTGGCTCTAA TTCCCAAATG GCTCAAGTCG GTGACGGTGA TAATTCACCT TTAATGAATA ATTTCCGTCA ATATTTACCT TCCCTCCCTC AATCGGTTGA ATGTCGCCCT TTTGTCTTTG GCCCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA GCGGGCAGTG AGCGCAACGC AATTAATGTG AGTTAGCTCA CTCATTAGGC ACCCCAGGCT TTACACTTTA TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGAGCGGATA ACAATTTCAC ACAGGAAACA GCTATGACCA TGATTACGCC AAGCTGGCGC GCCAAGCTTG TCACTGGATT TTGGTTTTAG GAATTAGAAA TTTTATTGAT AGAAGTATTT TACAAATACA AATACATACT AAGGGTTTCT TATATGCTCA ACACATGAGC GAAACCCTAT AAGAACCCTA ATTCCCTTAT CTGGGAACTA CTCACACATT ATTCTGGAGA AAAATAGAGA GAGATAGATT TGTAGAGAGA GACTGGTGAT TTTTGCGGAC TCTAGTTCAT CTAGAGGATC CGTCGACCCC GGGGGTACCC TTGTACAGCT CGTCCATGCC GAGAGTGATC CCGGCGGCGG TCACGAACTC CAGCAGGACC ATGTGATCGC GCTTCTCGTT GGGGTCTTTG CTCAGGGCGG ACTGGGTGCT CAGGTAGTGG TTGTCGGGCA GCAGCACGGG GCCGTCGCCG ATGGGGGTGT TCTGCTGGTA GTGGTCGGCG AGCTGCACGC TGCCGTCCTC GATGTTGTGG CGGATCTTGA AGTTCACCTT GATGCCGTTC TTCTGCTTGT CGGCCATGAT ATAGACGTTG TGGCTGTTGT AGTTGTACTC CAGCTTGTGC CCCAGGATGT TGCCGTCCTC CTTGAAGTCG ATGCCCTTCA GCTCGATGCG GTTCACCAGG GTGTCGCCCT CGAACTTCAC CTCGGCGCGG GTCTTGTAGT TGCCGTCGTC CTTGAAGAAG ATGGTGCGCT CCTGGACGTA GCCTTCGGGC ATGGCGGACT TGAAGAAGTC GTGCTGCTTC ATGTGGTCGG GGTAGCGGCT GAAGCACTGC ACGCCGTAGG TCAGGGTGGT CACGAGGGTG GGCCAGGGCA CGGGCAGCTT GCCGGTGGTG CAGATGAACT TCAGGGTCAG CTTGCCGTAG GTGGCATCGC CCTCGCCCTC GCCGGACACG CTGAACTTGT GGCCGTTTAC GTCGCCGTCC AGCTCGACCA GGATGGGCAC CACCCCGGTG AACAGCTCCT CGCCCTTGCT CACCATGGCT ATCGTTCGTA AATGGTGAAA ATTTTCAGAA AATTGCTTTT GCTTTAAAAG AAATGATTTA AATTGCTGCA ATAGAAGTAG AATGCTTGAT TGCTTGAGAT TCGTTTGTTT TGTATATGTT GTGTTGAGAA TTCTCGAGGT CCTCTCCAAA TGAAATGAAC TTCCTTATAT AGAGGAAGGG TCTTGCGAAG GATAGTGGGA TTGTGCGTCA TCCCTTACGT CAGTGGAGAT ATCACATCAA TCCACTTGCT TTGAAGACGT GGTTGGAACG TCTTCTTTTT CCACGATGCT CCTCGTGGGT GGGGGTCCAT CTTTGGGACC ACTGTCGGCA GAGGCATCTT CAACGATGGC CTTTCCTTTA TCGCAATGAT GGCATTTGTA GGAGCCACCT TCCTTTTCCA CTATCTTCAC AATAAAGTGA CAGATAGCTG GGCAATGGAA TCCGAGGAGG TTTCCGGATA TTACCCTTTG TTGAAAAGTC TCAATTGCCC TTTGGTCTTC TGAGACTGTA TCTTTGATAT TTTTGGAGTA GACAAGTGTG TCGTGCTCCA CCATGTTATC ACATCAATCC ACTTGCTTTG AAGACGTGGT TGGAACGTCT TCTTTTTCCA CGATGCTCCT CGTGGGTGGG GGTCCATCTT TGGGACCACT GTCGGCAGAG GCATCTTCAA CGATGGCCTT TCCTTTATCG CAATGATGGC ATTTGTAGGA GCCACCTTCC TTTTCCACTA TCTTCACAAT AAAGTGACAG ATAGCTGGGC AATGGAATCC GAGGAGGTTT CCGGATATTA CCCTTTGTTG AAAAGTCTCA ATTGCCCTTT GGTCTTCTGA GACTGTATCT TTGATATTTT TGGAGTAGAC AAGTGTGTCG TGCTCCACCA TGTTGACCTG CAGGCATGCA AGGAGCTCGA ATTCTTAATT AACAATTCAC TGGCCGTCGT TTTACAACGT CGTGACTGGG AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA TCCCCCTTTC GCCAGCTGGC GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA GTTGCGCAGC CTGAATGGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTTGGGTGA TGGTTCACAA ACTATCAGTG TTTGACAGGA TATATTGGCG GGTAAACCTA AGAGAAAAGA GCGTTTATTA GAATAATCGG ATATTTAAAA GGGCGTGAAA AGGTTTATCC GTTCGTCCAT TTGTATGTGC ATGCCAACCA CAGGGTTCCC CAGATCTGGC GCCGGCCAGC GAGACGAGCA AGATTGGCCG CCGCCCGAAA CGATCCGACA GCGCGCCCAG CACAGGTGCG CAGGCAAATT GCACCAACGC ATACAGCGCC AGCAGAATGC CATAGTGGGC GGTGACGTCG TTCGAGTGAA CCAGATCGCG CAGGAGGCCC GGCAGCACCG GCATAATCAG GCCGATGCCG ACAGCGTCGA GCGCGACAGT GCTCAGAATT ACGATCAGGG GTATGTTGGG TTTCACGTCT GGCCTCCGGA CCAGCCTCCG CTGGTCCGAT TGAACGCGCG GATTCTTTAT CACTGATAAG TTGGTGGACA TATTATGTTT ATCAGTGATA AAGTGTCAAG CATGACAAAG TTGCAGCCGA ATACAGTGAT CCGTGCCGCC CTGGACCTGT TGAACGAGGT CGGCGTAGAC GGTCTGACGA CACGCAAACT GGCGGAACGG TTGGGGGTTC AGCAGCCGGC GCTTTACTGG CACTTCAGGA ACAAGCGGGC GCTGCTCGAC GCACTGGCCG AAGCCATGCT GGCGGAGAAT CATACGCATT CGGTGCCGAG AGCCGACGAC GACTGGCGCT CATTTCTGAT CGGGAATGCC CGCAGCTTCA GGCAGGCGCT GCTCGCCTAC CGCGATGGCG CGCGCATCCA TGCCGGCACG CGACCGGGCG CACCGCAGAT GGAAACGGCC GACGCGCAGC TTCGCTTCCT CTGCGAGGCG GGTTTTTCGG CCGGGGACGC CGTCAATGCG CTGATGACAA TCAGCTACTT CACTGTTGGG GCCGTGCTTG AGGAGCAGGC CGGCGACAGC GATGCCGGCG AGCGCGGCGG CACCGTTGAA CAGGCTCCGC TCTCGCCGCT GTTGCGGGCC GCGATAGACG CCTTCGACGA AGCCGGTCCG GACGCAGCGT TCGAGCAGGG ACTCGCGGTG ATTGTCGATG GATTGGCGAA AAGGAGGCTC GTTGTCAGGA ACGTTGAAGG ACCGAGAAAG GGTGACGATT GATCAGGACC GCTGCCGGAG CGCAACCCAC TCACTACAGC AGAGCCATGT AGACAACATC CCCTCCCCCT TTCCACCGCG TCAGACGCCC GTAGCAGCCC GCTACGGGCT TTTTCATGCC CTGCCCTAGC GTCCAAGCCT CACGGCCGCG CTCGGCCTCT CTGGCGGCCT TCTGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TTCCGCTGCA TAACCCTGCT TCGGGGTCAT TATAGCGATT TTTTCGGTAT ATCCATCCTT TTTCGCACGA TATACAGGAT TTTGCCAAAG GGTTCGTGTA GACTTTCCTT GGTGTATCCA ACGGCGTCAG CCGGGCAGGA TAGGTGAAGT AGGCCCACCC GCGAGCGGGT GTTCCTTCTT CACTGTCCCT TATTCGCACC TGGCGGTGCT CAACGGGAAT CCTGCTCTGC GAGGCTGGCC GGCTACCGCC GGCGTAACAG ATGAGGGCAA GCGGATGGCT GATGAAACCA AGCCAACCAG GAAGGGCAGC CCACCTATCA AGGTGTACTG CCTTCCAGAC GAACGAAGAG CGATTGAGGA AAAGGCGGCG GCGGCCGGCA TGAGCCTGTC GGCCTACCTG CTGGCCGTCG GCCAGGGCTA CAAAATCACG GGCGTCGTGG ACTATGAGCA CGTCCGCGAG CTGGCCCGCA TCAATGGCGA CCTGGGCCGC CTGGGCGGCC TGCTGAAACT CTGGCTCACC GACGACCCGC GCACGGCGCG GTTCGGTGAT GCCACGATCC TCGCCCTGCT GGCGAAGATC GAAGAGAAGC AGGACGAGCT TGGCAAGGTC ATGATGGGCG TGGTCCGCCC GAGGGCAGAG CCATGACTTT TTTAGCCGCT AAAACGGCCG GGGGGTGCGC GTGATTGCCA AGCACGTCCC CATGCGCTCC ATCAAGAAGA GCGACTTCGC GGAGCTGGTG AAGTACATCA CCGACGAGCA AGGCAAGACC GAGCGCCTTT GCGACGCTCA
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