pKD46-Tet
$99.00
In stock
SKU
PL84766
100μg liquid plasmid(Instantly):$134
| name | pKD46-Tet | another name | apKD46-Tet |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Gene editing | Fragment type | Red |
| Fragment species | Prokaryotic resistance | Tet | |
| Selective marker | Fluorescent labeling | ||
| Promoter | araBAD | Replicon | pSC101 |
| Competence | DH5a | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | PBAD30.F：AGATTAGCGGATCCTACCTG | |
| 3′ sequencing primer | pKD46-R：TCTAGCAGAATTTACAGATACCC | Copy number | low |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CATCGATTTA TTATGACAAC TTGACGGCTA CATCATTCAC TTTTTCTTCA CAACCGGCAC GGAACTCGCT CGGGCTGGCC CCGGTGCATT TTTTAAATAC CCGCGAGAAA TAGAGTTGAT CGTCAAAACC AACATTGCGA CCGACGGTGG CGATAGGCAT CCGGGTGGTG CTCAAAAGCA GCTTCGCCTG GCTGATACGT TGGTCCTCGC GCCAGCTTAA GACGCTAATC CCTAACTGCT GGCGGAAAAG ATGTGACAGA CGCGACGGCG ACAAGCAAAC ATGCTGTGCG ACGCTGGCGA TATCAAAATT GCTGTCTGCC AGGTGATCGC TGATGTACTG ACAAGCCTCG CGTACCCGAT TATCCATCGG TGGATGGAGC GACTCGTTAA TCGCTTCCAT GCGCCGCAGT AACAATTGCT CAAGCAGATT TATCGCCAGC AGCTCCGAAT AGCGCCCTTC CCCTTGCCCG GCGTTAATGA TTTGCCCAAA CAGGTCGCTG AAATGCGGCT GGTGCGCTTC ATCCGGGCGA AAGAACCCCG TATTGGCAAA TATTGACGGC CAGTTAAGCC ATTCATGCCA GTAGGCGCGC GGACGAAAGT AAACCCACTG GTGATACCAT TCGCGAGCCT CCGGATGACG ACCGTAGTGA TGAATCTCTC CTGGCGGGAA CAGCAAAATA TCACCCGGTC GGCAAACAAA TTCTCGTCCC TGATTTTTCA CCACCCCCTG ACCGCGAATG GTGAGATTGA GAATATAACC TTTCATTCCC AGCGGTCGGT CGATAAAAAA ATCGAGATAA CCGTTGGCCT CAATCGGCGT TAAACCCGCC ACCAGATGGG CATTAAACGA GTATCCCGGC AGCAGGGGAT CATTTTGCGC TTCAGCCATA CTTTTCATAC TCCCGCCATT CAGAGAAGAA ACCAATTGTC CATATTGCAT CAGACATTGC CGTCACTGCG TCTTTTACTG GCTCTTCTCG CTAACCAAAC CGGTAACCCC GCTTATTAAA AGCATTCTGT AACAAAGCGG GACCAAAGCC ATGACAAAAA CGCGTAACAA AAGTGTCTAT AATCACGGCA GAAAAGTCCA CATTGATTAT TTGCACGGCG TCACACTTTG CTATGCCATA GCATTTTTAT CCATAAGATT AGCGGATCCT ACCTGACGCT TTTTATCGCA ACTCTCTACT GTTTCTCCAT ACCCGTTTTT TTGGGAATTC GAGCTCGGTA CCCTCGAGTA AGGAGGTTAT AAAAAATGGA TATTAATACT GAAACTGAGA TCAAGCAAAA GCATTCACTA ACCCCCTTTC CTGTTTTCCT AATCAGCCCG GCATTTCGCG GGCGATATTT TCACAGCTAT TTCAGGAGTT CAGCCATGAA CGCTTATTAC ATTCAGGATC GTCTTGAGGC TCAGAGCTGG GCGCGTCACT ACCAGCAGCT CGCCCGTGAA GAGAAAGAGG CAGAACTGGC AGACGACATG GAAAAAGGCC TGCCCCAGCA CCTGTTTGAA TCGCTATGCA TCGATCATTT GCAACGCCAC GGGGCCAGCA AAAAATCCAT TACCCGTGCG TTTGATGACG ATGTTGAGTT TCAGGAGCGC ATGGCAGAAC ACATCCGGTA CATGGTTGAA ACCATTGCTC ACCACCAGGT TGATATTGAT TCAGAGGTAT AAAACGAATG AGTACTGCAC TCGCAACGCT GGCTGGGAAG CTGGCTGAAC GTGTCGGCAT GGATTCTGTC GACCCACAGG AACTGATCAC CACTCTTCGC CAGACGGCAT TTAAAGGTGA TGCCAGCGAT GCGCAGTTCA TCGCATTACT GATCGTTGCC AACCAGTACG GCCTTAATCC GTGGACGAAA GAAATTTACG CCTTTCCTGA TAAGCAGAAT GGCATCGTTC CGGTGGTGGG CGTTGATGGC TGGTCCCGCA TCATCAATGA AAACCAGCAG TTTGATGGCA TGGACTTTGA GCAGGACAAT GAATCCTGTA CATGCCGGAT TTACCGCAAG GACCGTAATC ATCCGATCTG CGTTACCGAA TGGATGGATG AATGCCGCCG CGAACCATTC AAAACTCGCG AAGGCAGAGA AATCACGGGG CCGTGGCAGT CGCATCCCAA ACGGATGTTA CGTCATAAAG CCATGATTCA GTGTGCCCGT CTGGCCTTCG GATTTGCTGG TATCTATGAC AAGGATGAAG CCGAGCGCAT TGTCGAAAAT ACTGCATACA CTGCAGAACG TCAGCCGGAA CGCGACATCA CTCCGGTTAA CGATGAAACC ATGCAGGAGA TTAACACTCT GCTGATCGCC CTGGATAAAA CATGGGATGA CGACTTATTG CCGCTCTGTT CCCAGATATT TCGCCGCGAC ATTCGTGCAT CGTCAGAACT GACACAGGCC GAAGCAGTAA AAGCTCTTGG ATTCCTGAAA CAGAAAGCCG CAGAGCAGAA GGTGGCAGCA TGACACCGGA CATTATCCTG CAGCGTACCG GGATCGATGT GAGAGCTGTC GAACAGGGGG ATGATGCGTG GCACAAATTA CGGCTCGGCG TCATCACCGC TTCAGAAGTT CACAACGTGA TAGCAAAACC CCGCTCCGGA AAGAAGTGGC CTGACATGAA AATGTCCTAC TTCCACACCC TGCTTGCTGA GGTTTGCACC GGTGTGGCTC CGGAAGTTAA CGCTAAAGCA CTGGCCTGGG GAAAACAGTA CGAGAACGAC GCCAGAACCC TGTTTGAATT CACTTCCGGC GTGAATGTTA CTGAATCCCC GATCATCTAT CGCGACGAAA GTATGCGTAC CGCCTGCTCT CCCGATGGTT TATGCAGTGA CGGCAACGGC CTTGAACTGA AATGCCCGTT TACCTCCCGG GATTTCATGA AGTTCCGGCT CGGTGGTTTC GAGGCCATAA AGTCAGCTTA CATGGCCCAG GTGCAGTACA GCATGTGGGT GACGCGAAAA AATGCCTGGT ACTTTGCCAA CTATGACCCG CGTATGAAGC GTGAAGGCCT GCATTATGTC GTGATTGAGC GGGATGAAAA GTACATGGCG AGTTTTGACG AGATCGTGCC GGAGTTCATC GAAAAAATGG ACGAGGCACT GGCTGAAATT GGTTTTGTAT TTGGGGAGCA ATGGCGATGA CGCATCCTCA CGATAATATC CGGGTAGGCG CAATCACTTT CGTCTACTCC GTTACAAAGC GAGGCTGGGT ATTTCCCGGC CTTTCTGTTA TCCGAAATCC ACTGAAAGCA CAGCGGCTGG CTGAGGAGAT AAATAATAAA CGAGGGGCTG TATGCACAAA GCATCTTCTG TTGAGTTAAG AACGAGTATC GAGATGGCAC ATAGCCTTGC TCAAATTGGA ATCAGGTTTG TGCCAATACC AGTAGAAACA GACGAAGAAT CCATGGGTAT GGACAGTTTT CCCTTTGATA TGTAACGGTG AACAGTTGTT CTACTTTTGT TTGTTAGTCT TGATGCTTCA CTGATAGATA CAAGAGCCAT AAGAACCTCA GATCCTTCCG TATTTAGCCA GTATGTTCTC TAGTGTGGTT CGTTGTTTTT GCGTGAGCCA TGAGAACGAA CCATTGAGAT CATACTTACT TTGCATGTCA CTCAAAAATT TTGCCTCAAA ACTGGTGAGC TGAATTTTTG CAGTTAAAGC ATCGTGTAGT GTTTTTCTTA GTCCGTTACG TAGGTAGGAA TCTGATGTAA TGGTTGTTGG TATTTTGTCA CCATTCATTT TTATCTGGTT GTTCTCAAGT TCGGTTACGA GATCCATTTG TCTATCTAGT TCAACTTGGA AAATCAACGT ATCAGTCGGG CGGCCTCGCT TATCAACCAC CAATTTCATA TTGCTGTAAG TGTTTAAATC TTTACTTATT GGTTTCAAAA CCCATTGGTT AAGCCTTTTA AACTCATGGT AGTTATTTTC AAGCATTAAC ATGAACTTAA ATTCATCAAG GCTAATCTCT ATATTTGCCT TGTGAGTTTT CTTTTGTGTT AGTTCTTTTA ATAACCACTC ATAAATCCTC ATAGAGTATT TGTTTTCAAA AGACTTAACA TGTTCCAGAT TATATTTTAT GAATTTTTTT AACTGGAAAA GATAAGGCAA TATCTCTTCA CTAAAAACTA ATTCTAATTT TTCGCTTGAG AACTTGGCAT AGTTTGTCCA CTGGAAAATC TCAAAGCCTT TAACCAAAGG ATTCCTGATT TCCACAGTTC TCGTCATCAG CTCTCTGGTT GCTTTAGCTA ATACACCATA AGCATTTTCC CTACTGATGT TCATCATCTG AGCGTATTGG TTATAAGTGA ACGATACCGT CCGTTCTTTC CTTGTAGGGT TTTCAATCGT GGGGTTGAGT AGTGCCACAC AGCATAAAAT TAGCTTGGTT TCATGCTCCG TTAAGTCATA GCGACTAATC GCTAGTTCAT TTGCTTTGAA AACAACTAAT TCAGACATAC ATCTCAATTG GTCTAGGTGA TTTTAATCAC TATACCAATT GAGATGGGCT AGTCAATGAT AATTACTAGT CCTTTTCCTT TGAGTTGTGG GTATCTGTAA ATTCTGCTAG ACCTTTGCTG GAAAACTTGT AAATTCTGCT AGACCCTCTG TAAATTCCGC TAGACCTTTG TGTGTTTTTT TTGTTTATAT TCAAGTGGTT ATAATTTATA GAATAAAGAA AGAATAAAAA AAGATAAAAA GAATAGATCC CAGCCCTGTG TATAACTCAC TACTTTAGTC AGTTCCGCAG TATTACAAAA GGATGTCGCA AACGCTGTTT GCTCCTCTAC AAAACAGACC TTAAAACCCT AAAGGCTTAA GTAGCACCCT CGCAAGCTCG GTTGCGGCCG CAATCGGGCA AATCGCTGAA TATTCCTTTT GTCTCCGACC ATCAGGCACC TGAGTCGCTG TCTTTTTCGT GACATTCAGT TCGCTGCGCT CACGGCTCTG GCAGTGAATG GGGGTAAATG GCACTACAGG CGCCTTTTAT GGATTCATGC AAGGAAACTA CCCATAATAC AAGAAAAGCC CGTCACGGGC TTCTCAGGGC GTTTTATGGC GGGTCTGCTA TGTGGTGCTA TCTGACTTTT TGCTGTTCAG CAGTTCCTGC CCTCTGATTT TCCAGTCTGA CCACTTCGGA TTATCCCGTG ACAGGTCATT CAGACTGGCT AATGCACCCA GTAAGGCAGC GGTATCATCA ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TTCGTCTTCA AGAATTCTCA TGTTTGACAG CTTATCATCG ATAAGCTTTA ATGCGGTAGT TTATCACAGT TAAATTGCTA ACGCAGTCAG GCACCGTGTA TGAAATCTAA CAATGCGCTC ATCGTCATCC TCGGCACCGT CACCCTGGAT GCTGTAGGCA TAGGCTTGGT TATGCCGGTA CTGCCGGGCC TCTTGCGGGA TATCGTCCAT TCCGACAGCA TCGCCAGTCA CTATGGCGTG CTGCTAGCGC TATATGCGTT GATGCAATTT CTATGCGCAC CCGTTCTCGG AGCACTGTCC GACCGCTTTG GCCGCCGCCC AGTCCTGCTC GCTTCGCTAC TTGGAGCCAC TATCGACTAC GCGATCATGG CGACCACACC CGTCCTGTGG ATCCTCTACG CCGGACGCAT CGTGGCCGGC ATCACCGGCG CCACAGGTGC GGTTGCTGGC GCCTATATCG CCGACATCAC CGATGGGGAA GATCGGGCTC GCCACTTCGG GCTCATGAGC GCTTGTTTCG GCGTGGGTAT GGTGGCAGGC CCCGTGGCCG GGGGACTGTT GGGCGCCATC TCCTTGCATG CACCATTCCT TGCGGCGGCG GTGCTCAACG GCCTCAACCT ACTACTGGGC TGCTTCCTAA TGCAGGAGTC GCATAAGGGA GAGCGTCGAC CGATGCCCTT GAGAGCCTTC AACCCAGTCA GCTCCTTCCG GTGGGCGCGG GGCATGACTA TCGTCGCCGC ACTTATGACT GTCTTCTTTA TCATGCAACT CGTAGGACAG GTGCCGGCAG CGCTCTGGGT CATTTTCGGC GAGGACCGCT TTCGCTGGAG CGCGACGATG ATCGGCCTGT CGCTTGCGGT ATTCGGAATC TTGCACGCCC TCGCTCAAGC CTTCGTCACT GGTCCCGCCA CCAAACGTTT CGGCGAGAAG CAGGCCATTA TCGCCGGCAT GGCGGCCGAC GCGCTGGGCT ACGTCTTGCT GGCGTTCGCG ACGCGAGGCT GGATGGCCTT CCCCATTATG ATTCTTCTCG CTTCCGGCGG CATCGGGATG CCCGCGTTGC AGGCCATGCT GTCCAGGCAG GTAGATGACG ACCATCAGGG ACAGCTTCAA GGATCGCTCG CGGCTCTTAC CAGCCTAACT TCGATCACTG GACCGCTGAT CGTCACGGCG ATTTATGCCG CCTCGGCGAG CACATGGAAC GGGTTGGCAT GGATTGTAGG CGCCGCCCTA TACCTTGTCT GCCTCCCCGC GTTGCGTCGC GGTGCATGGA GCCGGGCCAC CTCGACCTGA CACATTTCCC CGAAAAGTGC CACCTG
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