pGL4.27[luc2P minP Hygro]
$84.00
In stock
SKU
PL84754
100μg liquid plasmid(Instantly):$105
| name | pGL4.27[luc2P minP Hygro] | another name | apGL4.27[luc2P minP Hygro] |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Promoter detection | Fragment type | Promoter |
| Fragment species | Empty vector | Prokaryotic resistance | Amp |
| Selective marker | Hyg | Fluorescent labeling | Fluc |
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | RVP3：CTAGCAAAATAGGCTGTCCC | |
| 3′ sequencing primer | GLP4：GGGTAGAATGGCGCTGGG | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GGCCTAACTG GCCGGTACCT GAGCTCGCTA GCCTCGAGGA TATCAAGATC TGGCCTCGGC GGCCAAGCTT AGACACTAGA GGGTATATAA TGGAAGCTCG ACTTCCAGCT TGGCAATCCG GTACTGTTGG TAAAGCCACC ATGGAAGATG CCAAAAACAT TAAGAAGGGC CCAGCGCCAT TCTACCCACT CGAAGACGGG ACCGCCGGCG AGCAGCTGCA CAAAGCCATG AAGCGCTACG CCCTGGTGCC CGGCACCATC GCCTTTACCG ACGCACATAT CGAGGTGGAC ATTACCTACG CCGAGTACTT CGAGATGAGC GTTCGGCTGG CAGAAGCTAT GAAGCGCTAT GGGCTGAATA CAAACCATCG GATCGTGGTG TGCAGCGAGA ATAGCTTGCA GTTCTTCATG CCCGTGTTGG GTGCCCTGTT CATCGGTGTG GCTGTGGCCC CAGCTAACGA CATCTACAAC GAGCGCGAGC TGCTGAACAG CATGGGCATC AGCCAGCCCA CCGTCGTATT CGTGAGCAAG AAAGGGCTGC AAAAGATCCT CAACGTGCAA AAGAAGCTAC CGATCATACA AAAGATCATC ATCATGGATA GCAAGACCGA CTACCAGGGC TTCCAAAGCA TGTACACCTT CGTGACTTCC CATTTGCCAC CCGGCTTCAA CGAGTACGAC TTCGTGCCCG AGAGCTTCGA CCGGGACAAA ACCATCGCCC TGATCATGAA CAGTAGTGGC AGTACCGGAT TGCCCAAGGG CGTAGCCCTA CCGCACCGCA CCGCTTGTGT CCGATTCAGT CATGCCCGCG ACCCCATCTT CGGCAACCAG ATCATCCCCG ACACCGCTAT CCTCAGCGTG GTGCCATTTC ACCACGGCTT CGGCATGTTC ACCACGCTGG GCTACTTGAT CTGCGGCTTT CGGGTCGTGC TCATGTACCG CTTCGAGGAG GAGCTATTCT TGCGCAGCTT GCAAGACTAT AAGATTCAAT CTGCCCTGCT GGTGCCCACA CTATTTAGCT TCTTCGCTAA GAGCACTCTC ATCGACAAGT ACGACCTAAG CAACTTGCAC GAGATCGCCA GCGGCGGGGC GCCGCTCAGC AAGGAGGTAG GTGAGGCCGT GGCCAAACGC TTCCACCTAC CAGGCATCCG CCAGGGCTAC GGCCTGACAG AAACAACCAG CGCCATTCTG ATCACCCCCG AAGGGGACGA CAAGCCTGGC GCAGTAGGCA AGGTGGTGCC CTTCTTCGAG GCTAAGGTGG TGGACTTGGA CACCGGTAAG ACACTGGGTG TGAACCAGCG CGGCGAGCTG TGCGTCCGTG GCCCCATGAT CATGAGCGGC TACGTTAACA ACCCCGAGGC TACAAACGCT CTCATCGACA AGGACGGCTG GCTGCACAGC GGCGACATCG CCTACTGGGA CGAGGACGAG CACTTCTTCA TCGTGGACCG GCTGAAGAGC CTGATCAAAT ACAAGGGCTA CCAGGTAGCC CCAGCCGAAC TGGAGAGCAT CCTGCTGCAA CACCCCAACA TCTTCGACGC CGGGGTCGCC GGCCTGCCCG ACGACGATGC CGGCGAGCTG CCCGCCGCAG TCGTCGTGCT GGAACACGGT AAAACCATGA CCGAGAAGGA GATCGTGGAC TATGTGGCCA GCCAGGTTAC AACCGCCAAG AAGCTGCGCG GTGGTGTTGT GTTCGTGGAC GAGGTGCCTA AAGGACTGAC CGGCAAGTTG GACGCCCGCA AGATCCGCGA GATTCTCATT AAGGCCAAGA AGGGCGGCAA GATCGCCGTG AATTCTCACG GCTTCCCTCC CGAGGTGGAG GAGCAGGCCG CCGGCACCCT GCCCATGAGC TGCGCCCAGG AGAGCGGCAT GGATAGACAC CCTGCTGCTT GCGCCAGCGC CAGGATCAAC GTCTAAGGCC GCGACTCTAG AGTCGGGGCG GCCGGCCGCT TCGAGCAGAC ATGATAAGAT ACATTGATGA GTTTGGACAA ACCACAACTA GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT TTATTTGTAA CCATTATAAG CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT ATGTTTCAGG TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTAAAA TCGATAAGGA TCCGTTTGCG TATTGGGCGC TCTTCCGCTG ATCTGCGCAG CACCATGGCC TGAAATAACC TCTGAAAGAG GAACTTGGTT AGCTACCTTC TGAGGCGGAA AGAACCAGCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCTGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCGATTCTT CTGACACTAG CGCCACCATG AAGAAGCCCG AACTCACCGC TACCAGCGTT GAAAAATTTC TCATCGAGAA GTTCGACAGT GTGAGCGACC TGATGCAGTT GTCGGAGGGC GAAGAGAGCC GAGCCTTCAG CTTCGATGTC GGCGGACGCG GCTATGTACT GCGGGTGAAT AGCTGCGCTG ATGGCTTCTA CAAAGACCGC TACGTGTACC GCCACTTCGC CAGCGCTGCA CTACCCATCC CCGAAGTGTT GGACATCGGC GAGTTCAGCG AGAGCCTGAC ATACTGCATC AGTAGACGCG CCCAAGGCGT TACTCTCCAA GACCTCCCCG AAACAGAGCT GCCTGCTGTG TTACAGCCTG TCGCCGAAGC TATGGATGCT ATTGCCGCCG CCGACCTCAG TCAAACCAGC GGCTTCGGCC CATTCGGGCC CCAAGGCATC GGCCAGTACA CAACCTGGCG GGATTTCATT TGCGCCATTG CTGATCCCCA TGTCTACCAC TGGCAGACCG TGATGGACGA CACCGTGTCC GCCAGCGTAG CTCAAGCCCT GGACGAACTG ATGCTGTGGG CCGAAGACTG TCCCGAGGTG CGCCACCTCG TCCATGCCGA CTTCGGCAGC AACAACGTCC TGACCGACAA CGGCCGCATC ACCGCCGTAA TCGACTGGTC CGAAGCTATG TTCGGGGACA GTCAGTACGA GGTGGCCAAC ATCTTCTTCT GGCGGCCCTG GCTGGCTTGC ATGGAGCAGC AGACTCGCTA CTTCGAGCGC CGGCATCCCG AGCTGGCCGG CAGCCCTCGT CTGCGAGCCT ACATGCTGCG CATCGGCCTG GATCAGCTCT ACCAGAGCCT CGTGGACGGC AACTTCGACG ATGCTGCCTG GGCTCAAGGC CGCTGCGATG CCATCGTCCG CAGCGGGGCC GGCACCGTCG GTCGCACACA AATCGCTCGC CGGAGCGCAG CCGTATGGAC CGACGGCTGC GTCGAGGTGC TGGCCGACAG CGGCAACCGC CGGCCCAGTA CACGACCGCG CGCTAAGGAG GTAGGTCGAG TTTAAACTCT AGAACCGGTC ATGGCCGCAA TAAAATATCT TTATTTTCAT TACATCTGTG TGTTGGTTTT TTGTGTGTTC GAACTAGATG CTGTCGACCG ATGCCCTTGA GAGCCTTCAA CCCAGTCAGC TCCTTCCGGT GGGCGCGGGG CATGACTATC GTCGCCGCAC TTATGACTGT CTTCTTTATC ATGCAACTCG TAGGACAGGT GCCGGCAGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGCGGCCGCA AATGCTAAAC CACTGCAGTG GTTACCAGTG CTTGATCAGT GAGGCACCGA TCTCAGCGAT CTGCCTATTT CGTTCGTCCA TAGTGGCCTG ACTCCCCGTC GTGTAGATCA CTACGATTCG TGAGGGCTTA CCATCAGGCC CCAGCGCAGC AATGATGCCG CGAGAGCCGC GTTCACCGGC CCCCGATTTG TCAGCAATGA ACCAGCCAGC AGGGAGGGCC GAGCGAAGAA GTGGTCCTGC TACTTTGTCC GCCTCCATCC AGTCTATGAG CTGCTGTCGT GATGCTAGAG TAAGAAGTTC GCCAGTGAGT AGTTTCCGAA GAGTTGTGGC CATTGCTACT GGCATCGTGG TATCACGCTC GTCGTTCGGT ATGGCTTCGT TCAACTCTGG TTCCCAGCGG TCAAGCCGGG TCACATGATC ACCCATATTA TGAAGAAATG CAGTCAGCTC CTTAGGGCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCG GTGTTGTCGC TCATGGTAAT GGCAGCACTA CACAATTCTC TTACCGTCAT GCCATCCGTA AGATGCTTTT CCGTGACCGG CGAGTACTCA ACCAAGTCGT TTTGTGAGTA GTGTATACGG CGACCAAGCT GCTCTTGCCC GGCGTCTATA CGGGACAACA CCGCGCCACA TAGCAGTACT TTGAAAGTGC TCATCATCGG GAATCGTTCT TCGGGGCGGA AAGACTCAAG GATCTTGCCG CTATTGAGAT CCAGTTCGAT ATAGCCCACT CTTGCACCCA GTTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCGGG GTGTGCAAAA ACAGGCAAGC AAAATGCCGC AAAGAAGGGA ATGAGTGCGA CACGAAAATG TTGGATGCTC ATACTCGTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTACTAGTA CGTCTCTCAA GGATAAGTAA GTAATATTAA GGTACGGGAG GTATTGGACA GGCCGCAATA AAATATCTTT ATTTTCATTA CATCTGTGTG TTGGTTTTTT GTGTGAATCG ATAGTACTAA CATACGCTCT CCATCAAAAC AAAACGAAAC AAAACAAACT AGCAAAATAG GCTGTCCCCA GTGCAAGTGC AGGTGCCAGA ACATTTCTCT
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