pYES2-UCP1-EGFP
$99.00
In stock
SKU
PL84608
NM_021833.5
100μg liquid plasmid(Instantly):$120
| name | pYES2-UCP1-EGFP | another name | apYES2-UCP1-EGFP |
| Insert Size (bp) | 921bp | Plasmid host | Yeast bacterium |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | URA3 | Fluorescent labeling | Green |
| Promoter | GAL1 | Replicon | pUC,2U |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GCCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGCG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATTCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGGCATTGC TACAGGCATC GTGGTGTCAC TCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATAGTGTAT CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATGGGTAAT AACTGATATA ATTAAATTGA AGCTCTAATT TGTGAGTTTA GTATACATGC ATTTACTTAT AATACAGTTT TTTAGTTTTG CTGGCCGCAT CTTCTCAAAT ATGCTTCCCA GCCTGCTTTT CTGTAACGTT CACCCTCTAC CTTAGCATCC CTTCCCTTTG CAAATAGTCC TCTTCCAACA ATAATAATGT CAGATCCTGT AGAGACCACA TCATCCACGG TTCTATACTG TTGACCCAAT GCGTCTCCCT TGTCATCTAA ACCCACACCG GGTGTCATAA TCAACCAATC GTAACCTTCA TCTCTTCCAC CCATGTCTCT TTGAGCAATA AAGCCGATAA CAAAATCTTT GTCGCTCTTC GCAATGTCAA CAGTACCCTT AGTATATTCT CCAGTAGATA GGGAGCCCTT GCATGACAAT TCTGCTAACA TCAAAAGGCC TCTAGGTTCC TTTGTTACTT CTTCTGCCGC CTGCTTCAAA CCGCTAACAA TACCTGGGCC CACCACACCG TGTGCATTCG TAATGTCTGC CCATTCTGCT ATTCTGTATA CACCCGCAGA GTACTGCAAT TTGACTGTAT TACCAATGTC AGCAAATTTT CTGTCTTCGA AGAGTAAAAA ATTGTACTTG GCGGATAATG CCTTTAGCGG CTTAACTGTG CCCTCCATGG AAAAATCAGT CAAGATATCC ACATGTGTTT TTAGTAAACA AATTTTGGGA CCTAATGCTT CAACTAACTC CAGTAATTCC TTGGTGGTAC GAACATCCAA TGAAGCACAC AAGTTTGTTT GCTTTTCGTG CATGATATTA AATAGCTTGG CAGCAACAGG ACTAGGATGA GTAGCAGCAC GTTCCTTATA TGTAGCTTTC GACATGATTT ATCTTCGTTT CCTGCAGGTT TTTGTTCTGT GCAGTTGGGT TAAGAATACT GGGCAATTTC ATGTTTCTTC AACACTACAT ATGCGTATAT ATACCAATCT AAGTCTGTGC TCCTTCCTTC GTTCTTCCTT CTGTTCGGAG ATTACCGAAT CAAAAAAATT TCAAAGAAAC CGAAATCAAA AAAAAGAATA AAAAAAAAAT GATGAATTGA ATTGAAAAGC TAGCTTATCG ATGATAAGCT GTCAAAGATG AGAATTAATT CCACGGACTA TAGACTATAC TAGATACTCC GTCTACTGTA CGATACACTT CCGCTCAGGT CCTTGTCCTT TAACGAGGCC TTACCACTCT TTTGTTACTC TATTGATCCA GCTCAGCAAA GGCAGTGTGA TCTAAGATTC TATCTTCGCG ATGTAGTAAA ACTAGCTAGA CCGAGAAAGA GACTAGAAAT GCAAAAGGCA CTTCTACAAT GGCTGCCATC ATTATTATCC GATGTGACGC TGCAGCTTCT CAATGATATT CGAATACGCT TTGAGGAGAT ACAGCCTAAT ATCCGACAAA CTGTTTTACA GATTTACGAT CGTACTTGTT ACCCATCATT GAATTTTGAA CATCCGAACC TGGGAGTTTT CCCTGAAACA GATAGTATAT TTGAACCTGT ATAATAATAT ATAGTCTAGC GCTTTACGGA AGACAATGTA TGTATTTCGG TTCCTGGAGA AACTATTGCA TCTATTGCAT AGGTAATCTT GCACGTCGCA TCCCCGGTTC ATTTTCTGCG TTTCCATCTT GCACTTCAAT AGCATATCTT TGTTAACGAA GCATCTGTGC TTCATTTTGT AGAACAAAAA TGCAACGCGA GAGCGCTAAT TTTTCAAACA AAGAATCTGA GCTGCATTTT TACAGAACAG AAATGCAACG CGAAAGCGCT ATTTTACCAA CGAAGAATCT GTGCTTCATT TTTGTAAAAC AAAAATGCAA CGCGACGAGA GCGCTAATTT TTCAAACAAA GAATCTGAGC TGCATTTTTA CAGAACAGAA ATGCAACGCG AGAGCGCTAT TTTACCAACA AAGAATCTAT ACTTCTTTTT TGTTCTACAA AAATGCATCC CGAGAGCGCT ATTTTTCTAA CAAAGCATCT TAGATTACTT TTTTTCTCCT TTGTGCGCTC TATAATGCAG TCTCTTGATA ACTTTTTGCA CTGTAGGTCC GTTAAGGTTA GAAGAAGGCT ACTTTGGTGT CTATTTTCTC TTCCATAAAA AAAGCCTGAC TCCACTTCCC GCGTTTACTG ATTACTAGCG AAGCTGCGGG TGCATTTTTT CAAGATAAAG GCATCCCCGA TTATATTCTA TACCGATGTG GATTGCGCAT ACTTTGTGAA CAGAAAGTGA TAGCGTTGAT GATTCTTCAT TGGTCAGAAA ATTATGAACG GTTTCTTCTA TTTTGTCTCT ATATACTACG TATAGGAAAT GTTTACATTT TCGTATTGTT TTCGATTCAC TCTATGAATA GTTCTTACTA CAATTTTTTT GTCTAAAGAG TAATACTAGA GATAAACATA AAAAATGTAG AGGTCGAGTT TAGATGCAAG TTCAAGGAGC GAAAGGTGGA TGGGTAGGTT ATATAGGGAT ATAGCACAGA GATATATAGC AAAGAGATAC TTTTGAGCAA TGTTTGTGGA AGCGGTATTC GCAATGGGAA GCTCCACCCC GGTTGATAAT CAGAAAAGCC CCAAAAACAG GAAGATTGTA TAAGCAAATA TTTAAATTGT AAACGTTAAT ATTTTGTTAA AATTCGCGTT AAATTTTTGT TAAATCAGCT CATTTTTTAA CGAATAGCCC GAAATCGGCA AAATCCCTTA TAAATCAAAA GAATAGACCG AGATAGGGTT GAGTGTTGTT CCAGTTTCCA ACAAGAGTCC ACTATTAAAG AACGTGGACT CCAACGTCAA AGGGCGAAAA AGGGTCTATC AGGGCGATGG CCCACTACGT GAACCATCAC CCTAATCAAG TTTTTTGGGG TCGAGGTGCC GTAAAGCAGT AAATCGGAAG GGTAAACGGA TGCCCCCATT TAGAGCTTGA CGGGGAAAGC CGGCGAACGT GGCGAGAAAG GAAGGGAAGA AAGCGAAAGG AGCGGGGGCT AGGGCGGTGG GAAGTGTAGG GGTCACGCTG GGCGTAACCA CCACACCCGC CGCGCTTAAT GGGGCGCTAC AGGGCGCGTG GGGATGATCC ACTAGTACGG ATTAGAAGCC GCCGAGCGGG TGACAGCCCT CCGAAGGAAG ACTCTCCTCC GTGCGTCCTC GTCCTCACCG GTCGCGTTCC TGAAACGCAG ATGTGCCTCG CGCCGCACTG CTCCGAACAA TAAAGATTCT ACAATACTAG CTTTTATGGT TATGAAGAGG AAAAATTGGC AGTAACCTGG CCCCACAAAC CTTCAAATGA ACGAATCAAA TTAACAACCA TAGGATGATA ATGCGATTAG TTTTTTAGCC TTATTTCTGG GGTAATTAAT CAGCGAAGCG ATGATTTTTG ATCTATTAAC AGATATATAA ATGCAAAAAC TGCATTAACC ACTTTAACTA ATACTTTCAA CATTTTCGGT TTGTATTACT TCTTATTCAA ATGTAATAAA AGTATCAACA AAAAATTGTT AATATACCTC TATACTTTAA CGTCAAGGAG AAAAAACCCC GGATCGGACT ACTAGCAGCT GTAATACGAC TCACTATAGG GAATATTAAG CTTATGGGGG GCCTGACAGC CTCGGACGTA CACCCGACCC TGGGGGTCCA GCTCTTCTCA GCTGGAATAG CGGCGTGCTT GGCGGACGTG ATCACCTTCC CGCTGGACAC GGCCAAAGTC CGGCTCCAGG TCCAAGGTGA ATGCCCGACG TCCAGTGTTA TTAGGTATAA AGGTGTCCTG GGAACAATCA CCGCTGTGGT AAAAACAGAA GGGCGGATGA AACTCTACAG CGGGCTGCCT GCGGGGCTTC AGCGGCAAAT CAGCTCCGCC TCTCTCAGGA TCGGCCTCTA CGACACGGTC CAGGAGTTCC TCACCGCAGG GAAAGAAACA GCACCTAGTT TAGGAAGCAA GATTTTAGCT GGTCTAACGA CTGGAGGAGT GGCAGTATTC ATTGGGCAAC CCACAGAGGT CGTGAAAGTC AGACTTCAAG CACAGAGCCA TCTCCACGGA ATCAAACCTC GCTACACGGG GACTTATAAT GCGTACAGAA TAATAGCAAC AACCGAAGGC TTGACGGGTC TTTGGAAAGG GACTACTCCC AATCTGATGA GAAGTGTCAT CATCAATTGT ACAGAGCTAG TAACATATGA TCTAATGAAG GAGGCCTTTG TGAAAAACAA CATATTAGCA GATGACGTCC CCTGCCACTT GGTGTCGGCT CTTATCGCTG GATTTTGCGC AACAGCTATG TCCTCCCCGG TGGATGTAGT AAAAACCAGA TTTATTAATT CTCCACCAGG ACAGTACAAA AGTGTGCCCA ACTGTGCAAT GAAAGTGTTC ACTAACGAAG GACCAACGGC TTTCTTCAAG GGGTTGGTAC CTTCCTTCTT GCGACTTGGA TCCTGGAACG TCATTATGTT TGTGTGCTTT GAACAACTGA AACGAGAACT GTCAAAGTCA AGGCAGACTA TGGACTGTGC CACAGGCGGC GGAGGGAGTG GAGGAGGGGG AAGCGGAGGC GGAGGGAGTG AATTCTGCAG ATATCCATCA CACTGGCGGC CGCTCGAGAT GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG CATCAAGGTG AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCACC CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGTAATC TAGAGGGCCG CATCATGTAA TTAGTTATGT CACGCTTACA TTCACGCCCT CCCCCCACAT CCGCTCTAAC CGAAAAGGAA GGAGTTAGAC AACCTGAAGT CTAGGTCCCT ATTTATTTTT TTATAGTTAT GTTAGTATTA AGAACGTTAT TTATATTTCA AATTTTTCTT TTTTTTCTGT ACAGACGCGT GTACGCATGT AACATTATAC TGAAAACCTT GCTTGAGAAG GTTTTGGGAC GCTCGAAGGC TTTAATTTGC GGCC
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
