NM_015073.3;CTRCT45; SPAL3; SPAR3
100μg liquid plasmid(Instantly):$163
| name | pECMV-SIPA1L3(human)-3×FLAG(3synonymous mutations) | another name | apECMV-SIPA1L3(human)-3×FLAG(3synonymous mutations) |
| Insert Size (bp) | 5343bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCTTGGTA CCGAGCTCGG ATCCGCCACC ATGACCACCT ATCGGGCCAT CCCCAGCGAT GGTGTGGACC TGGCAGCCAG CTGTGGCGCC AGGGTGGGCG ATGTCCTCCC TGGGCCACAC ACAGGGGACT ACGCTCCCTT GGGATTCTGG GCCCAGAATG GCAGCATGTC CCAGCCTCTT GGCGAGAGCC CGGCCACCGC CACCGCCACC GCCACCGCCA CCACCCGCCC CAGCCCCACC ACTCCCGCAA TGCCCAAGAT GGGCGTGCGC GCAAGGGTGG CCGACTGGCC GCCCAAGCGG GAGGCCCTGA GAGAGCACAG CAACCCAAGC CCCTCCCAGG ACACAGATGG CACAAAGGCC ACCAAGATGG CCCATTCCAT GAGGAGCATA CAGAACGGAC AGCCCCCCAC CAGCACCCCG GCTTCCTCAG GGTCCAAAGC CTTCCACCGA CTCTCCAGGA GAAGGTCCAA AGACGTGGAG TTCCAGGACG GGTGGCCCCG GTCCCCCGGC AGGGCCTTCC TCCCCCTTCG GCACCGCAGC AGCAGCGAGA TCACCCTCAG CGAGTGTGAC GCGGAGGACG CGGGGGAGCC GCGGGGGGCC CGGCACACGG GGGCGCTGCC CCTCTTCCGC GAGTACGGGA GCACCTCGTC CATCGACGTG CAGGGCATGC CCGAGCAGAG CTTCTTCGAC ATCCTGAACG AGTTCCGCAG CGAGCAGCCC GACGCCCGAG GGTGCCAGGC CCTCACCGAG CTCCTCCGGG CAGATCCTGG CCCACACCTC ATGGGGGGCG GCGGCGGAGC CAAGGGGGAC TCCCACAACG GGCAGCCCGC CAAGGACAGC CTCCTGCCAC TGCAGCCCAC GAAGGAGAAG GAGAAGGCCC GGAAGAAACC TGCGCGGGGC CTCGGCGGCG GGGACACGGT GGACTCGTCC ATCTTTCGGA AGCTAAGGAG CAGCAAACCC GAGGGGGAGG CTGGGCGTTC CCCGGGGGAG GCCGACGAGG GCCGGAGCCC CCCGGAAGCC AGCAGGCCGT GGGTGTGTCA GAAGAGCTTC GCCCACTTCG ACGTGCAGAG CATGCTGTTC GACCTCAACG AGGCGGCCGC CAACAGGGTG TCGGTGTCGC AGCGGCGGAA CACCACCACG GGTGCTTCGG CCGCTTCCGC CGCCTCGGCC ATGGCCTCCC TCACGGCCTC GCGGGCCCAC AGCCTCGGAG GCCTGGACCC GGCCTTCACC AGCACAGAGG ACCTAAACTG CAAGGAGAAC TTGGAGCAGG ACCTCGGCGA TGACAACAGC AACGACCTGC TGCTCAGCTG CCCGCACTTC CGCAATGAGA TCGGGGGCGA GTGTGAGCGC AACGTGAGCT TCTCCCGGGC TTCCGTGGGC TCCCCGAGCA GCGGCGAGGG CCACCTGGCA GAGCCCGCCC TGAGCGCCTA CCGCACCAAC GCCAGCATCT CGGTGTTGGA AGTTCCCAAG GAGCAGCAGC GGACGCAGAG TCGGCCCCGG CAGTACAGCA TCGAGCATGT GGACCTGGGC GCCCGCTACT ACCAGGATTA CTTCGTGGGC AAAGAACATG CCAATTACTT CGGCGTGGAT GAGAAGCTGG GGCCAGTGGC TGTGAGCATT AAGCGGGAGA AGCTGGAAGA CCACAAGGAG CACGGACCTC AGTACCAGTA CAGGATCATC TTCCGGACCC GCGAGCTCAT CACCCTGCGG GGCTCCATCC TGGAAGATGC TACGCCCACA GCCACCAAGC ATGGGACCGG GCGGGGCCTG CCCTTGAAGG ATGCCCTGGA GTATGTCATC CCCGAGCTCA ACATCCACTG CCTGCGGCTG GCCCTCAACA CCCCCAAGGT GACGGAGCAA CTGCTGAAGC TCGATGAGCA AGGGCTCTGC CGGAAGCACA AGGTGGGCAT CCTCTATTGC AAGGCCGGCC AGAGCTCCGA GGAGGAGATG TACAACAATG AGGAGGCCGG CCCCGCCTTT GAGGAGTTCC TCTCCCTCAT CGGCGAGAAG GTCTGCCTGA AGGGCTTCAC CAAGTACGCT GCCCAGCTGG ACGTCAAGAC CGACTCCACG GGAACCCACT CCCTCTACAC GATGTACCAG GACTACGAGA TCATGTTCCA TGTCTCCACC CTGCTCCCTT ACACCCCCAA CAACAGGCAG CAGCTGCTAC GGAAGAGGCA CATAGGAAAT GACATCGTGA CGATCATCTT CCAGGAGCCT GGCGCGCTAC CGTTCACCCC CAAGAACATC CGCTCCCACT TCCAGCACGT CTTCATCATT GTCCGAGTCC ACAACCCCTG CACTGATAAC GTCTGTTACA GTATGGCTGT GACCCGATCC AAAGACGCTC CTCCTTTCGG CCCCCCCATC CCCAGTGGAA CCACATTCCG CAAATCCGAC GTCTTCAGAG ACTTCTTGCT GGCCAAGGTG ATTAACGCTG AGAACGCCGC GCACAAGTCC GACAAGTTCC ACACCATGGC CACCAGGACC CGCCAGGAGT ATCTCAAGGA CCTGGCCGAA AACTGTGTCT CCAACACCCC CATCGACTCC ACCGGCAAAT TCAACCTCAT CTCCCTGACC TCCAAGAAGA AGGAAAAGAC AAAAGCACGG GCTGGCGCTG AGCAGCACAG TGCAGGGGCC ATCGCCTGGA GGGTGGTGGC CCAGGACTAC GCCCAGGGGG TGGAAATCGA CTGCATTTTG GGAATTTCCA ATGAGTTTGT GGTGCTCCTG GACTTACGCA CCAAGGAGGT GGTGTTCAAC TGCTACTGCG GGGATGTCAT TGGCTGGACT CCAGACTCCT CCACACTCAA AATCTTCTAT GGACGAGGAG ACCACATCTT CCTACAGGCG ACAGAGGGTT CTGTGGAGGA CATAAGGGAG ATAGTGCAGA GACTGAAGGT GATGACCAGT GGCTGGGAGA CGGTGGACAT GACGCTTCGG CGGAACGGGC TCGGGCAGCT GGGCTTCCAC GTGAAGTACG ACGGCACGGT GGCCGAGGTT GAGGACTATG GGTTCGCCTG GCAGGCCGGC CTCCGGCAGG GCAGCCGACT AGTGGAGATC TGCAAGGTGG CCGTGGTCAC ACTGACCCAC GACCAGATGA TCGACCTGCT GCGCACCTCT GTCACTGTGA AGGTGGTCAT CATCCCGCCT TTTGAGGACG GCACTCCCCG GAGGGGTTGG CCGGAGACCT ACGACATGAA TACCTCGGAG CCCAAGACGG AGCAGGAAAG CATCACTCCT GGGGGCCGGC CCCCCTACCG CAGCAATGCT CCCTGGCAGT GGAGCGGGCC CGCATCCCAT AACTCTCTAC CAGCCTCCAA GTGGGCCACT CCAACCACTC CCGGCCATGC CCAGTCCCTG AGCCGGCCCC TGAAGCAGAC CCCCATAGTC CCCTTCCGGG AGTCCCAGCC ACTGCACAGC AAGAGGCCTG TCAGCTTCCC AGAAACCCCT TACACAGTAT CACCAGCAGG GGCCGACAGA GTCCCTCCCT ACCGACAGCC TTCTGGGAGC TTCTCCACCC CCGGTTCGGC CACCTACGTG AGATACAAGC CATCCCCAGA AAGGTACACG GCTGCCCCAC ACCCCCTGCT ATCTCTTGAT CCCCACTTCA GCCACGATGG GACGTCCAGC GGCGACTCCT CTTCCGGCGG CCTGACCAGC CAGGAGAGCA CCATGGAACG CCAGAAGCCA GAGCCTTTGT GGCATGTGCC TGCCCAGGCC AGGCTCTCAG CCATAGCCGG AAGCAGCGGG AACAAGCACC CGTCCAGGCA GGATGCAGCA GGCAAAGATT CCCCCAACAG GCATTCCAAA GGAGAACCTC AATACTCAAG TCATTCCAGC AGCAACACCC TCTCCAGCAA CGCATCCAGC AGCCACAGCG ACGACCGCTG GTTCGACCCC CTGGACCCCC TGGAGCCAGA GCAAGACCCC CTCTCCAAGG GTGGCTCTAG TGACAGCGGC ATCGACACCA CCCTCTACAC CTCCAGCCCT AGCTGCATGT CCCTGGCCAA GGCTCCACGG CCCGCCAAGC CACACAAGCC CCCTGGAAGT ATGGGCCTTT GTGGCGGGGG TCGCGAGGCC GCTGGGAGGT CCCACCACGC AGACAGGCGG CGGGAGGTCT CCCCTGCCCC CGCAGTTGCC GGCCAAAGCA AGGGCTACCG ACCGAAGCTG TACTCCTCCG GCTCCAGCAC CCCCACGGGA CTGGCGGGGG GCAGCCGAGA CCCACCGAGG CAGCCCAGTG ACATGGGCTC GAGGGTTGGC TACCCCGCTC AGGTTTACAA AACTGCCAGT GCAGAGACTC CTCGGCCCTC CCAGCTGGCC CAGCCCAGCC CCTTTCAGCT CTCCGCCTCC GTCCCCAAGT CCTTCTTCTC CAAGCAGCCT GTACGCAATA AGCACCCAAC AGGGTGGAAG AGAACGGAGG AGCCCCCACC ACGGCCACTC CCCTTCAGTG ACCCAAAGAA GCAGGTGGAC ACGAACACCA AAAATGTCTT TGGGCAACCG AGGTTGAGGG CATCCCTCCG AGACCTCCGG TCACCACGGA AGAACTACAA ATCCACCATC GAGGATGACC TGAAGAAACT CATCATCATG GACAACCTGG GGCCAGAGCA GGAGAGAGAC ACGGGACAGT CACCGCAGAA GGGCCTGCAG CGGACGCTGT CGGACGAGAG CCTGTGCAGC GGGCGCCGGG AGCCCAGCTT CGCCAGCCCC GCTGGCCTAG AGCCAGGGCT GCCCAGCGAC GTGCTCTTCA CCAGCACCTG CGCCTTCCCG TCCAGCACGC TGCCTGCACG CCGCCAGCAC CAGCACCCCC ACCCGCCCGT CGGCCCCGGT GCCACCCCTG CCGCCGGCAG CGGCTTTCCC GAGAAGAAAT CCACCATCTC AGCCTCGGAG CTCTCGCTGG CTGATGGGCG GGACCGCCCC CTGCGGCGCC TGGACCCTGG GCTGATGCCC CTGCCTGACA CAGCTGCTGG CCTCGAGTGG TCCAGCCTGG TGAACGCAGC CAAGGCATAC GAAGTGCAAA GAGCCGTCTC ACTCTTCTCT CTGAACGACC CGGCCCTGAG CCCGGACATC CCGCCTGCAC ACAGTCCTGT CCACAGCCAC CTGAGCCTGG AGAGGGGACC CCCGACCCCC AGGACCACCC CTACCATGAG CGAGGAGCCA CCCCTGGATC TGACAGGCAA GGTGTACCAG CTGGAGGTGA TGCTGAAACA GCTGCACACT GACCTGCAGA AGGAGAAGCA GGACAAGGTG GTGCTCCAGT CAGAGGTGGC CAGCCTGCGG CAGAACAACC AGCGGCTGCA GGAGGAGTCG CAGGCCGCCA GCGAGCAGCT GCGCAAGTTT GCGGAGATCT TCTGCAGGGA GAAGAAGGAG CTCCTCGAGG ACTACAAAGA CCATGACGGT GATTATAAAG ATCATGACAT CGACTACAAG GATGACGATG ACAAGTAGTG AGGGCCCGTT TAAACCCGCT GATCAGCCTC GACTGTGCCT TCTAGTTGCC AGCCATCTGT TGTTTGCCCC TCCCCCGTGC CTTCCTTGAC CCTGGAAGGT GCCACTCCCA CTGTCCTTTC CTAATAAAAT GAGGAAATTG CATCGCATTG TCTGAGTAGG TGTCATTCTA TTCTGGGGGG TGGGGTGGGG CAGGACAGCA AGGGGGAGGA TTGGGAAGAC AATAGCAGGC ATGCTGGGGA TGCGGTGGGC TCTATGGCTT CTGAGGCGGA AAGAACCAGC TGGGGCTCTA GGGGGTATCC CCACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TAATTCTGTG GAATGTGTGT CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCTGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT TCGAAATGAC CGACCAAGCG ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTC
