pCS2-TRAF7-R371G(human)
$99.00
In stock
SKU
PL84196
NM_032271.2;CAFDADD; RFWD1; RNF119
100μg liquid plasmid(Instantly):$120
| name | pCS2-TRAF7-R371G(human) | another name | apCS2-TRAF7-R371G(human) |
| Insert Size (bp) | 2013bp | Plasmid host | Mammary cell,Fish |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F： | |
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CAACTGTTGG GAAGGGCGAT CGGTGCGGGC CTCTTCGCTA TTACGCCAGT CGACCATAGC CAATTCAATA TGGCGTATAT GGACTCATGC CAATTCAATA TGGTGGATCT GGACCTGTGC CAATTCAATA TGGCGTATAT GGACTCGTGC CAATTCAATA TGGTGGATCT GGACCCCAGC CAATTCAATA TGGCGGACTT GGCACCATGC CAATTCAATA TGGCGGACTT GGCACTGTGC CAACTGGGGA GGGGTCTACT TGGCACGGTG CCAAGTTTGA GGAGGGGTCT TGGCCCTGTG CCAAGTCCGC CATATTGAAT TGGCATGGTG CCAATAATGG CGGCCATATT GGCTATATGC CAGGATCAAT ATATAGGCAA TATCCAATAT GGCCCTATGC CAATATGGCT ATTGGCCAGG TTCAATACTA TGTATTGGCC CTATGCCATA TAGTATTCCA TATATGGGTT TTCCTATTGA CGTAGATAGC CCCTCCCAAT GGGCGGTCCC ATATACCATA TATGGGGCTT CCTAATACCG CCCATAGCCA CTCCCCCATT GACGTCAATG GTCTCTATAT ATGGTCTTTC CTATTGACGT CATATGGGCG GTCCTATTGA CGTATATGGC GCCTCCCCCA TTGACGTCAA TTACGGTAAA TGGCCCGCCT GGCTCAATGC CCATTGACGT CAATAGGACC ACCCACCATT GACGTCAATG GGATGGCTCA TTGCCCATTC ATATCCGTTC TCACGCCCCC TATTGACGTC AATGACGGTA AATGGCCCAC TTGGCAGTAC ATCAATATCT ATTAATAGTA ACTTGGCAAG TACATTACTA TTGGAAGGAC GCCAGGGTAC ATTGGCAGTA CTCCCATTGA CGTCAATGGC GGTAAATGGC CCGCGATGGC TGCCAAGTAC ATCCCCATTG ACGTCAATGG GGAGGGGCAA TGACGCAAAT GGGCGTTCCA TTGACGTAAA TGGGCGGTAG GCGTGCCTAA TGGGAGGTCT ATATAAGCAA TGCTCGTTTA GGGAACCGCC ATTCTGCCTG GGGACGTCGG AGCAAGCTTG ATTTAGGTGA CACTATAGAA TACAAGCTAC TTGTTCTTTT TGCAGGATCC GCCACCATGA GCTCAGGCAA GAGTGCCCGC TACAACCGCT TCTCCGGGGG GCCCAGCAAT CTTCCCACCC CAGACGTCAC CACAGGGACC AGAATGGAAA CGACCTTCGG ACCCGCCTTT TCAGCCGTCA CCACCATCAC AAAAGCTGAC GGGACCAGCA CCTACAAGCA GCACTGCAGG ACACCCTCCT CCTCCAGCAC CCTTGCCTAC TCCCCGCGGG ACGAGGAGGA CAGCATGCCC CCCATCAGCA CTCCCCGCCG CTCCGACTCC GCCATCTCTG TCCGCTCCCT GCACTCAGAG TCCAGCATGT CTCTGCGCTC CACATTCTCA CTGCCCGAGG AGGAGGAGGA GCCGGAGCCA CTGGTGTTTG CGGAGCAGCC CTCGGTGAAG CTGTGCTGTC AGCTCTGCTG CAGCGTCTTC AAAGACCCCG TGATCACCAC GTGTGGGCAC ACGTTCTGTA GGAGATGCGC CTTGAAGTCA GAGAAGTGTC CCGTGGACAA CGTCAAACTG ACCGTGGTGG TGAACAACAT CGCGGTGGCC GAGCAGATCG GGGAGCTCTT CATCCACTGC CGGCACGGCT GCCGGGTAGC GGGCAGCGGG AAGCCCCCCA TCTTTGAGGT GGACCCCCGA GGGTGCCCCT TCACCATCAA GCTCAGCGCC CGGAAGGACC ACGAGGGCAG CTGTGACTAC AGGCCTGTGC GGTGTCCCAA CAACCCCAGC TGCCCCCCGC TGCTCAGGAT GAACCTGGAG GCCCACCTCA AGGAGTGCGA GCACATCAAA TGCCCCCACT CCAAGTACGG GTGCACGTTC ATCGGGAACC AGGACACTTA CGAGACCCAC CTGGAGACTT GCCGCTTCGA GGGCCTGAAG GAGTTTCTGC AGCAGACGGA TGACCGCTTC CACGAGATGC ACGTGGCTCT GGCCCAGAAG GACCAGGAGA TCGCCTTCCT GCGCTCCATG CTGGGAAAGC TCTCGGAGAA GATCGACCAG CTAGAGAAGA GCCTGGAGCT CAAGTTTGAC GTCCTGGACG AAAACCAGAG CAAGCTCAGC GAGGACCTCA TGGAGTTCCG GCGGGACGCA TCCATGTTAA ATGACGAGCT GTCCCACATC AACGCGGGGC TGAACATGGG CATCCTAGGC TCCTACGACC CTCAGCAGAT CTTCAAGTGC AAAGGGACCT TTGTGGGCCA CCAGGGCCCT GTGTGGTGTC TCTGCGTCTA CTCCATGGGT GACCTGCTCT TCAGTGGCTC CTCTGACAAG ACCATCAAGG TGTGGGACAC ATGTACCACC TACAAGTGTC AGAAGACACT GGAGGGCCAT GATGGCATCG TGCTGGCTCT CTGCATCCAG GGGTGCAAAC TCTACAGCGG CTCTGCAGAC TGCACCATCA TTGTGTGGGA CATCCAGAAC CTGCAGAAGG TGAACACCAT CCGGGCCCAT GACAACCCGG TGTGCACGCT GGTCTCCTCA CACAACGTGC TCTTCAGCGG CTCCCTGAAG GCCATCAAGG TCTGGGACAT CGTGGGCACT GAGCTGAAGT TGAAGAAGGA GCTCACAGGC CTCAACCACT GGGTGCGGGC CCTGGTGGCT GCCCAGAGCT ACCTGTACAG CGGCTCCTAC CAGACAATCA AGATCTGGGA CATCCGAACC CTTGACTGCA TCCACGTCCT GCAGACGTCT GGTGGCAGCG TCTACTCCAT TGCTGTGACA AATCACCACA TTGTCTGTGG CACCTACGAG AACCTCATCC ACGTGTGGGA CATTGAGTCC AAGGAGCAGG TGCGGACCCT CACGGGCCAC GTGGGCACCG TGTATGCCCT GGCGGTCATC TCGACGCCAG ACCAGACCAA AGTCTTCAGT GCATCCTACG ACCGGTCCCT CAGGGTCTGG AGTATGGACA ACATGATCTG CACGCAGACC CTGCTGCGTC ACCAGGGCAG TGTCACCGCG CTGGCTGTGT CCCGGGGCCG ACTCTTCTCA GGGGCTGTGG ATAGCACTGT GAAGGTTTGG ACTTGCTAAG AATTCAAGGC CTCTCGAGCC TCTAGAACTA TAGTGAGTCG TATTACGTAG ATCCAGACAT GATAAGATAC ATTGATGAGT TTGGACAAAC CACAACTAGA ATGCAGTGAA AAAAATGCTT TATTTGTGAA ATTTGTGATG CTATTGCTTT ATTTGTAACC ATTATAAGCT GCAATAAACA AGTTAACAAC AACAATTGCA TTCATTTTAT GTTTCAGGTT CAGGGGGAGG TGTGGGAGGT TTTTTAATTC GCGGCCGCGG CGCCAATGCA TTGGGCCCGG TACCCAGCTT TTGTTCCCTT TAGTGAGGGT TAATTGCGCG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTAAA TTGTAAGCGT TAATATTTTG TTAAAATTCG CGTTAAATTT TTGTTAAATC AGCTCATTTT TTAACCAATA GGCCGAAATC GGCAAAATCC CTTATAAATC AAAAGAATAG ACCGAGATAG GGTTGAGTGT TGTTCCAGTT TGGAACAAGA GTCCACTATT AAAGAACGTG GACTCCAACG TCAAAGGGCG AAAAACCGTC TATCAGGGCG ATGGCCCACT ACGTGAACCA TCACCCTAAT CAAGTTTTTT GGGGTCGAGG TGCCGTAAAG CACTAAATCG GAACCCTAAA GGGAGCCCCC GATTTAGAGC TTGACGGGGA AAGCCGGCGA ACGTGGCGAG AAAGGAAGGG AAGAAAGCGA AAGGAGCGGG CGCTAGGGCG CTGGCAAGTG TAGCGGTCAC GCTGCGCGTA ACCACCACAC CCGCCGCGCT TAATGCGCCG CTACAGGGCG CGTCCCATTC GCCATTCAGG CTGCG
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
