pGWB11
$128.00
In stock
SKU
PL84175
100μg liquid plasmid(Instantly):$149
| name | pGWB11 | another name | apGWB11 |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Protein expression | Fragment type | ORF,GateWay |
| Fragment species | Empty vector | Prokaryotic resistance | Kan |
| Selective marker | Neo/G418,Hyg | Fluorescent labeling | |
| Promoter | 35s | Replicon | oriV |
| Competence | DB3.1 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGAGCGTCGC AAAGGCGCTC GGTCTTGCCT TGCTCGTCGG TGATGTACTT CACCAGCTCC GCGAAGTCGC TCTTCTTGAT GGAGCGCATG GGGACGTGCT TGGCAATCAC GCGCACCCCC CGGCCGTTTT AGCGGCTAAA AAAGTCATGG CTCTGCCCTC GGGCGGACCA CGCCCATCAT GACCTTGCCA AGCTCGTCCT GCTTCTCTTC GATCTTCGCC AGCAGGGCGA GGATCGTGGC ATCACCGAAC CGCGCCGTGC GCGGGTCGTC GGTGAGCCAG AGTTTCAGCA GGCCGCCCAG GCGGCCCAGG TCGCCATTGA TGCGGGCCAG CTCGCGGACG TGCTCATAGT CCACGACGCC CGTGATTTTG TAGCCCTGGC CGACGGCCAG CAGGTAGGCC GACAGGCTCA TGCCGGCCGC CGCCGCCTTT TCCTCAATCG CTCTTCGTTC GTCTGGAAGG CAGTACACCT TGATAGGTGG GCTGCCCTTC CTGGTTGGCT TGGTTTCATC AGCCATCCGC TTGCCCTCAT CTGTTACGCC GGCGGTAGCC GGCCAGCCTC GCAGAGCAGG ATTCCCGTTG AGCACCGCCA GGTGCGAATA AGGGACAGTG AAGAAGGAAC ACCCGCTCGC GGGTGGGCCT ACTTCACCTA TCCTGCCCGG CTGACGCCGT TGGATACACC AAGGAAAGTC TACACGAACC CTTTGGCAAA ATCCTGTATA TCGTGCGAAA AAGGATGGAT ATACCGAAAA AATCGCTATA ATGACCCCGA AGCAGGGTTA TGCAGCGGAA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCAGA AGGCCGCCAG AGAGGCCGAG CGCGGCCGTG AGGCTTGGAC GCTAGGGCAG GGCATGAAAA AGCCCGTAGC GGGCTGCTAC GGGCGTCTGA CGCGGTGGAA AGGGGGAGGG GATGTTGTCT ACATGGCTCT GCTGTAGTGA GTGGGTTGCG CTCCGGCAGC GGTCCTGATC AATCGTCACC CTTTCTCGGT CCTTCAACGT TCCTGACAAC GAGCCTCCTT TTCGCCAATC CATCGACAAT CACCGCGAGT CCCTGCTCGA ACGCTGCGTC CGGACCGGCT TCGTCGAAGG CGTCTATCGC GGCCCGCAAC AGCGGCGAGA GCGGAGCCTG TTCAACGGTG CCGCCGCGCT CGCCGGCATC GCTGTCGCCG GCCTGCTCCT CAAGCACGGC CCCAACAGTG AAGTAGCTGA TTGTCATCAG CGCATTGACG GCGTCCCCGG CCGAAAAACC CGCCTCGCAG AGGAAGCGAA GCTGCGCGTC GGCCGTTTCC ATCTGCGGTG CGCCCGGTCG CGTGCCGGCA TGGATGCGCG CGCCATCGCG GTAGGCGAGC AGCGCCTGCC TGAAGCTGCG GGCATTCCCG ATCAGAAATG AGCGCCAGTC GTCGTCGGCT CTCGGCACCG AATGCGTATG ATTCTCCGCC AGCATGGCTT CGGCCAGTGC GTCGAGCAGC GCCCGCTTGT TCCTGAAGTG CCAGTAAAGC GCCGGCTGCT GAACCCCCAA CCGTTCCGCC AGTTTGCGTG TCGTCAGACC GTCTACGCCG ACCTCGTTCA ACAGGTCCAG GGCGGCACGG ATCACTGTAT TCGGCTGCAA CTTTGTCATG CTTGACACTT TATCACTGAT AAACATAATA TGTCCACCAA CTTATCAGTG ATAAAGAATC CGCGCGTTCA ATCGGACCAG CGGAGGCTGG TCCGGAGGCC AGACGTGAAA CCCAACATAC CCCTGATCGT AATTCTGAGC ACTGTCGCGC TCGACGCTGT CGGCATCGGC CTGATTATGC CGGTGCTGCC GGGCCTCCTG CGCGATCTGG TTCACTCGAA CGACGTCACC GCCCACTATG GCATTCTGCT GGCGCTGTAT GCGTTGGTGC AATTTGCCTG CGCACCTGTG CTGGGCGCGC TGTCGGATCG TTTCGGGCGG CGGCCAATCT TGCTCGTCTC GCTGGCCGGC GCCAGATCTG GGGAACCCTG TGGTTGGCAT GCACATACAA ATGGACGAAC GGATAAACCT TTTCACGCCC TTTTAAATAT CCGATTATTC TAATAAACGC TCTTTTCTCT TAGGTTTACC CGCCAATATA TCCTGTCAAA CACTGATAGT TTAAACTGAA GGCGGGAAAC GACAATCTGA TCATGAGCGG AGAATTAAGG GAGTCACGTT ATGACCCCCG CCGATGACGC GGGACAAGCC GTTTTACGTT TGGAACTGAC AGAACCGCAA CGTTGAAGGA GCCACTCAGC CGCGGGTTTC TGGAGTTTAA TGAGCTAAGC ACATACGTCA GAAACCATTA TTGCGCGTTC AAAAGTCGCC TAAGGTCACT ATCAGCTAGC AAATATTTCT TGTCAAAAAT GCTCCACTGA CGTTCCATAA ATTCCCCTCG GTATCCAATT AGAGTCTCAT ATTCACTCTC AATCCAAATA ATCTGCACCG GATCTGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GATGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACGGGATCTC TGCGGAACAG GCGGTCGAAG GTGCCGATAT CATTACGACA GCAACGGCCG ACAAGCACAA CGCCACGATC CTGAGCGACA ATATGATCGG GCCCGGCGTC CACATCAACG GCGTCGGCGG CGACTGCCCA GGCAAGACCG AGATGCACCG CGATATCTTG CTGCGTTCGG ATATTTTCGT GGAGTTCCCG CCACAGACCC GGATGATCCC CGATCGTTCA AACATTTGGC AATAAAGTTT CTTAAGATTG AATCCTGTTG CCGGTCTTGC GATGATTATC ATATAATTTC TGTTGAATTA CGTTAAGCAT GTAATAATTA ACATGTAATG CATGACGTTA TTTATGAGAT GGGTTTTTAT GATTAGAGTC CCGCAATTAT ACATTTAATA CGCGATAGAA AACAAAATAT AGCGCGCAAA CTAGGATAAA TTATCGCGCG CGGTGTCATC TATGTTACTA GATCGGGCCT CCTGTCAATG CTGGCGGCGG CTCTGGTGGT GGTTCTGGTG GCGGCTCTGA GGGTGGTGGC TCTGAGGGTG GCGGTTCTGA GGGTGGCGGC TCTGAGGGAG GCGGTTCCGG TGGTGGCTCT GGTTCCGGTG ATTTTGATTA TGAAAAGATG GCAAACGCTA ATAAGGGGGC TATGACCGAA AATGCCGATG AAAACGCGCT ACAGTCTGAC GCTAAAGGCA AACTTGATTC TGTCGCTACT GATTACGGTG CTGCTATCGA TGGTTTCATT GGTGACGTTT CCGGCCTTGC TAATGGTAAT GGTGCTACTG GTGATTTTGC TGGCTCTAAT TCCCAAATGG CTCAAGTCGG TGACGGTGAT AATTCACCTT TAATGAATAA TTTCCGTCAA TATTTACCTT CCCTCCCTCA ATCGGTTGAA TGTCGCCCTT TTGTCTTTGG CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGCCA AGCTTGCATG CCTGCAGGTC CCCAGATTAG CCTTTTCAAT TTCAGAAAGA ATGCTAACCC ACAGATGGTT AGAGAGGCTT ACGCAGCAGG TCTCATCAAG ACGATCTACC CGAGCAATAA TCTCCAGGAA ATCAAATACC TTCCCAAGAA GGTTAAAGAT GCAGTCAAAA GATTCAGGAC TAACTGCATC AAGAACACAG AGAAAGATAT ATTTCTCAAG ATCAGAAGTA CTATTCCAGT ATGGACGATT CAAGGCTTGC TTCACAAACC AAGGCAAGTA ATAGAGATTG GAGTCTCTAA AAAGGTAGTT CCCACTGAAT CAAAGGCCAT GGAGTCAAAG ATTCAAATAG AGGACCTAAC AGAACTCGCC GTAAAGACTG GCGAACAGTT CATACAGAGT CTCTTACGAC TCAATGACAA GAAGAAAATC TTCGTCAACA TGGTGGAGCA CGACACACTT GTCTACTCCA AAAATATCAA AGATACAGTC TCAGAAGACC AAAGGGCAAT TGAGACTTTT CAACAAAGGG TAATATCCGG AAACCTCCTC GGATTCCATT GCCCAGCTAT CTGTCACTTT ATTGTGAAGA TAGTGGAAAA GGAAGGTGGC TCCTACAAAT GCCATCATTG CGATAAAGGA AAGGCCATCG TTGAAGATGC CTCTGCCGAC AGTGGTCCCA AAGATGGACC CCCACCCACG AGGAGCATCG TGGAAAAAGA AGACGTTCCA ACCACGTCTT CAAAGCAAGT GGATTGATGT GATATCTCCA CTGACGTAAG GGATGACGCA CAATCCCACT ATCCTTCGCA AGACCCTTCC TCTATATAAG GAAGTTCATT TCATTTGGAG AGAACACGGG GGACTCTAGA GTTATCAACA AGTTTGTACA AAAAAGCTGA ACGAGAAACG TAAAATGATA TAAATATCAA TATATTAAAT TAGATTTTGC ATAAAAAACA GACTACATAA TACTGTAAAA CACAACATAT CCAGTCACTA TGGCGGCCCC TCGAGGGGCC GCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATAATGT GTGGATTTTG AGTTAGGATC CGGCGAGATT TTCAGGAGCT AAGGAAGCTA AAATGGAGAA AAAAATCACT GGATATACCA CCGTTGATAT ATCCCAATGG CATCGTAAAG AACATTTTGA GGCATTTCAG TCAGTTGCTC AATGTACCTA TAACCAGACC GTTCAGCTGG ATATTACGGC CTTTTTAAAG ACCGTAAAGA AAAATAAGCA CAAGTTTTAT CCGGCCTTTA TTCACATTCT TGCCCGCCTG ATGAATGCTC ATCCGGAATT CCGTATGGCA ATGAAAGACG GTGAGCTGGT GATATGGGAT AGTGTTCACC CTTGTTACAC CGTTTTCCAT GAGCAAACTG AAACGTTTTC ATCGCTCTGG AGTGAATACC ACGACGATTT CCGGCAGTTT CTACACATAT ATTCGCAAGA TGTGGCGTGT TACGGTGAAA ACCTGGCCTA TTTCCCTAAA GGGTTTATTG AGAATATGTT TTTCGTCTCA GCCAATCCCT GGGTGAGTTT CACCAGTTTT GATTTAAACG TGGCCAATAT GGACAACTTC TTCGCCCCCG TTTTCACCAT GGGCAAATAT TATACGCAAG GCGACAAGGT GCTGATGCCG CTGGCGATTC AGGTTCATCA TGCCGTCTGT GATGGCTTCC ATGTCGGCAG AATGCTTAAT GAATTACAAC AGTACTGCGA TGAGTGGCAG GGCGGGGCGT AAAGATCTGG ATCCGGCTTA CTAAAAGCCA GATAACAGTA TGCGTATTTG CGCGCTGATT TTTGCGGTAT AAGAATATAT ACTGATATGT ATACCCGAAG TATGTCAAAA AGAGGTGTGC TATGAAGCAG CGTATTACAG TGACAGTTGA CAGCGACAGC TATCAGTTGC TCAAGGCATA TATGATGTCA ATATCTCCGG TCTGGTAAGC ACAACCATGC AGAATGAAGC CCGTCGTCTG CGTGCCGAAC GCTGGAAAGC GGAAAATCAG GAAGGGATGG CTGAGGTCGC CCGGTTTATT GAAATGAACG GCTCTTTTGC TGACGAGAAC AGGGACTGGT GAAATGCAGT TTAAGGTTTA CACCTATAAA AGAGAGAGCC GTTATCGTCT GTTTGTGGAT GTACAGAGTG ATATTATTGA CACGCCCGGG CGACGGATGG TGATCCCCCT GGCCAGTGCA CGTCTGCTGT CAGATAAAGT CTCCCGTGAA CTTTACCCGG TGGTGCATAT CGGGGATGAA AGCTGGCGCA TGATGACCAC CGATATGGCC AGTGTGCCGG TCTCCGTTAT CGGGGAAGAA GTGGCTGATC TCAGCCACCG CGAAAATGAC ATCAAAAACG CCATTAACCT GATGTTCTGG GGAATATAAA TGTCAGGCTC CCTTATACAC AGCCAGTCTG CAGGTCGACC ATAGTGACTG GATATGTTGT GTTTTACAGT ATTATGTAGT CTGTTTTTTA TGCAAAATCT AATTTAATAT ATTGATATTT ATATCATTTT ACGTTTCTCG TTCAGCTTTC TTGTACAAAG TGGTTGATAA CAGCGACTAC AAGGATGACG ATGACAAGGC TTAGAGCTCG AATTTCCCCG ATCGTTCAAA CATTTGGCAA TAAAGTTTCT TAAGATTGAA TCCTGTTGCC GGTCTTGCGA TGATTATCAT ATAATTTCTG TTGAATTACG TTAAGCATGT AATAATTAAC ATGTAATGCA TGACGTTATT TATGAGATGG GTTTTTATGA TTAGAGTCCC GCAATTATAC ATTTAATACG CGATAGAAAA CAAAATATAG CGCGCAAACT AGGATAAATT ATCGCGCGCG GTGTCATCTA TGTTACTAGA TCGGGAATTA GCTTCATCAA CGCAAGACAT GCGCACGACC GTCTGACAGG AGAGGAATTT CCGACGAGCA CAGAAAGGAC TTGCTCTTGG ACGTAGGCCT ATTTCTCAGG CACATGTATC AAGTGTTCGG ACGTGGGTTT TCGATGGTGT ATCAGCCGCC GCCAACTGGG AGATGAGGAG GCTTTCTTGG GGGGCAGTCA GCAGTTCATT TCACAAGACA GAGGAACTTG TAAGGAGATG CACTGATTTA TCTTGGCGCA AACCAGCAGG ACGAATTAGT GGGAATAGCC CGCGAATATC TAAGTTATGC CTGTCGGCAT GAGCAGAAAC TTCCAATTCG AAACAGTTTG GAGAGGTTGT TTTTGGGCAT ACCTTTTGTT AGTCAGCCTC TCGATTGCTC ATCGTCATTA CACAGTACCG AAGTTTGATC GATCTAGTAA CATAGATGAC ACCGCGCGCG ATAATTTATC CTAGTTTGCG CGCTATATTT TGTTTTCTAT CGCGTATTAA ATGTATAATT GCGGGACTCT AATCATAAAA ACCCATCTCA TAAATAACGT CATGCATTAC ATGTTAATTA TTACATGCTT AACGTAATTC AACAGAAATT ATATGATAAT CATCGCAAGA CCGGCAACAG GATTCAATCT TAAGAAACTT TATTGCCAAA TGTTTGAACG ATCTGCTTCG ACGCACTCCT TCTTTACTCC ACCATCTCGT CCTTATTGAA AACGTGGGTA GCACCAAAAC GAATCAAGTC GCTGGAACTG AAGTTACCAA TCACGCTGGA TGATTTGCCA GTTGGATTAA TCTTGCCTTT CCCCGCATGA ATAATATTGA TGAATGCATG CGTGAGGGGT ATTTCGATTT TGGCAATAGC TGCAATTGCC GCGACATCCT CCAACGAGCA TAATTCTTCA GAAAAATAGC GATGTTCCAT GTTGTCAGGG CATGCATGAT GCACGTTATG AGGTGACGGT GCTAGGCAGT ATTCCCTCAA AGTTTCATAG TCAGTATCAT ATTCATCATT GCATTCCTGC AAGAGAGAAT TGAGACGCAA TCCACACGCT GCGGCAACCT TCCGGCGTTC GTGGTCTATT TGCTCTTGGA CGTTGCAAAC GTAAGTGTTG GATCCCGGTC GGCATCTACT CTATTCCTTT GCCCTCGGAC GAGTGCTGGG GCGTCGGTTT CCACTATCGG CGAGTACTTC TACACAGCCA TCGGTCCAGA CGGCCGCGCT TCTGCGGGCG ATTTGTGTAC GCCCGACAGT CCCGGCTCCG GATCGGACGA TTGCGTCGCA TCGACCCTGC GCCCAAGCTG CATCATCGAA ATTGCCGTCA ACCAAGCTCT GATAGAGTTG GTCAAGACCA ATGCGGAGCA TATACGCCCG GAGCCGCGGC GATCCTGCAA GCTCCGGATG CCTCCGCTCG AAGTAGCGCG TCTGCTGCTC CATACAAGCC AACCACGGCC TCCAGAAGAA GATGTTGGCG ACCTCGTATT GGGAATCCCC GAACATCGCC TCGCTCCAGT CAATGACCGC TGTTATGCGG CCATTGTCCG TCAGGACATT GTTGGAGCCG AAATCCGCGT GCACGAGGTG CCGGACTTCG GGGCAGTCCT CGGCCCAAAG CATCAGCTCA TCGAGAGCCT GCGCGACGGA CGCACTGACG GTGTCGTCCA TCACAGTTTG CCAGTGATAC ACATGGGGAT CAGCAATCGC GCATATGAAA TCACGCCATG TAGTGTATTG ACCGATTCCT TGCGGTCCGA ATGGGCCGAA CCCGCTCGTC TGGCTAAGAT CGGCCGCAGC GATCGCATCC ATGGCCTCCG CGACCGGCTG CAGAACAGCG GGCAGTTCGG TTTCAGGCAG GTCTTGCAAC GTGACACCCT GTGCACGGCG GGAGATGCAA TAGGTCAGGC TCTCGCTGAA TTCCCCAATG TCAAGCACTT CCGGAATCGG GAGCGCGGCC GATGCAAAGT GCCGATAAAC ATAACGATCT TTGTAGAAAC CATCGGCGCA GCTATTTACC CGCAGGACAT ATCCACGCCC TCCTACATCG AAGCTGAAAG CACGAGATTC TTCGCCCTCC GAGAGCTGCA TCAGGTCGGA GACGCTGTCG AACTTTTCGA TCAGAAACTT CTCGACAGAC GTCGCGGTGA GTTCAGGCTT TTTCATATCG GGGTCGTCCT CTCCAAATGA AATGAACTTC CTTATATAGA GGAAGGGTCT TGCGAAGGAT AGTGGGATTG TGCGTCATCC CTTACGTCAG TGGAGATATC ACATCAATCC ACTTGCTTTG AAGACGTGGT TGGAACGTCT TCTTTTTCCA CGATGCTCCT CGTGGGTGGG GGTCCATCTT TGGGACCACT GTCGGCAGAG GCATCTTGAA CGATAGCCTT TCCTTTATCG CAATGATGGC ATTTGTAGGT GCCACCTTCC TTTTCTACTG TCCTTTTGAT GAAGTGACAG ATAGCTGGGC AATGGAATCC GAGGAGGTTT CCCGATATTA CCCTTTGTTG AAAAGTCTCA ATAGCCCTTT GGTCTTCTGA GACTGTATCT TTGATATTCT TGGAGTAGAC GAGAGTGTCG TGCTCCACCA TGTTGACGGA TCTCTAGGAC GCGTCCTAGA AGCTAATTCA CTGGCCGTCG TTTTACAACG TCGTGACTGG GAAAACCCTG GCGTTACCCA ACTTAATCGC CTTGCAGCAC ATCCCCCTTT CGCCAGCTGG CGTAATAGCG AAGAGGCCCG CACCGATCGC CCTTCCCAAC AGTTGCGCAG CCTGAATGGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTTGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG GCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGA ACCACCATCA AACAGGATTT TCGCCTGCTG GGGCAAACCA GCGTGGACCG CTTGCTGCAA CTCTCTCAGG GCCAGGCGGT GAAGGGCAAT CAGCTGTTGC CCGTCTCACT GGTGAAAAGA AAAACCACCC CAGTACATTA AAAACGTCCG CAATGTGTTA TTAAGTTGTC TAAGCGTCAA TTTGTTTACA CCACAATATA TCCTGCCACC AGCCAGCCAA CAGCTCCCCG ACCGGCAGCT CGGCACAAAA TCACCACTCG ATACAGGCAG CCCATCAGTC CGGGACGGCG TCAGCGGGAG AGCCGTTGTA AGGCGGCAGA CTTTGCTCAT GTTACCGATG CTATTCGGAA GAACGGCAAC TAAGCTGCCG GGTTTGAAAC ACGGATGATC TCGCGGAGGG TAGCATGTTG ATTGTAACGA TGACAGAGCG TTGCTGCCTG TGATCAAATA TCATCTCCCT CGCAGAGATC CGAATTATCA GCCTTCTTAT TCATTTCTCG CTTAACCGTG ACAGGCTGTC GATCTTGAGA ACTATGCCGA CATAATAGGA AATCGCTGGA TAAAGCCGCT GAGGAAGCTG AGTGGCGCTA TTTCTTTAGA AGTGAACGTT GACGATATCA ACTCCCCTAT CCATTGCTCA CCGAATGGTA CAGGTCGGGG ACCCGAAGTT CCGACTGTCG GCCTGATGCA TCCCCGGCTG ATCGACCCCA GATCTGGGGC TGAGAAAGCC CAGTAAGGAA ACAACTGTAG GTTCGAGTCG CGAGATCCCC CGGAACCAAA GGAAGTAGGT TAAACCCGCT CCGATCAGGC CGAGCCACGC CAGGCCGAGA ACATTGGTTC CTGTAGGCAT CGGGATTGGC GGATCAAACA CTAAAGCTAC TGGAACGAGC AGAAGTCCTC CGGCCGCCAG TTGCCAGGCG GTAAAGGTGA GCAGAGGCAC GGGAGGTTGC CACTTGCGGG TCAGCACGGT TCCGAACGCC ATGGAAACCG CCCCCGCCAG GCCCGCTGCG ACGCCGACAG GATCTAGCGC TGCGTTTGGT GTCAACACCA ACAGCGCCAC GCCCGCAGTT CCGCAAATAG CCCCCAGGAC CGCCATCAAT CGTATCGGGC TACCTAGCAG AGCGGCAGAG ATGAACACGA CCATCAGCGG CTGCACAGCG CCTACCGTCG CCGCGACCCC GCCCGGCAGG CGGTAGACCG AAATAAACAA CAAGCTCCAG AATAGCGAAA TATTAAGTGC GCCGAGGATG AAGATGCGCA TCCACCAGAT TCCCGTTGGA ATCTGTCGGA CGATCATCAC GAGCAATAAA CCCGCCGGCA ACGCCCGCAG CAGCATACCG GCGACCCCTC GGCCTCGCTG TTCGGGCTCC ACGAAAACGC CGGACAGATG CGCCTTGTGA GCGTCCTTGG GGCCGTCCTC CTGTTTGAAG ACCGACAGCC CAATGATCTC GCCGTCGATG TAGGCGCCGA ATGCCACGGC ATCTCGCAAC CGTTCAGCGA ACGCCTCCAT GGGCTTTTTC TCCTCGTGCT CGTAAACGGA CCCGAACATC TCTGGAGCTT TCTTCAGGGC CGACAATCGG ATCTCGCGGA AATCCTGCAC GTCGGCCGCT CCAAGCCGTC GAATCTGAGC CTTAATCACA ATTGTCAATT TTAATCCTCT GTTTATCGGC AGTTCGTAGA GCGCGCCGTG CGTCCCGAGC GATACTGAGC GAAGCAAGTG CGTCGAGCAG TGCCCGCTTG TTCCTGAAAT GCCAGTAAAG CGCTGGCTGC TGAACCCCCA GCCGGAACTG ACCCCACAAG GCCCTAGCGT TTGCAATGCA CCAGGTCATC ATTGACCCAG GCGTGTTCCA CCAGGCCGCT GCCTCGCAAC TCTTCGCAGG CTTCGCCGAC CTGCTCGCGC CACTTCTTCA CGCGGGTGGA ATCCGATCCG CACATGAGGC GGAAGGTTTC CAGCTTGAGC GGGTACGGCT CCCGGTGCGA GCTGAAATAG TCGAACATCC GTCGGGCCGT CGGCGACAGC TTGCGGTACT TCTCCCATAT GAATTTCGTG TAGTGGTCGC CAGCAAACAG CACGACGATT TCCTCGTCGA TCAGGACCTG GCAACGGGAC GTTTTCTTGC CACGGTCCAG GACGCGGAAG CGGTGCAGCA GCGACACCGA TTCCAGGTGC CCAACGCGGT CGGACGTGAA GCCCATCGCC GTCGCCTGTA GGCGCGACAG GCATTCCTCG GCCTTCGTGT AATACCGGCC ATTGATCGAC CAGCCCAGGT CCTGGCAAAG CTCGTAGAAC GTGAAGGTGA TCGGCTCGCC GATAGGGGTG CGCTTCGCGT ACTCCAACAC CTGCTGCCAC ACCAGTTCGT CATCGTCGGC CCGCAGCTCG ACGCCGGTGT AGGTGATCTT CACGTCCTTG TTGACGTGGA AAATGACCTT GTTTTGCAGC GCCTCGCGCG GGATTTTCTT GTTGCGCGTG GTGAACAGGG CAGAGCGGGC CGTGTCGTTT GGCATCGCTC GCATCGTGTC CGGCCACGGC GCAATATCGA ACAAGGAAAG CTGCATTTCC TTGATCTGCT GCTTCGTGTG TTTCAGCAAC GCGGCCTGCT TGGCCTCGCT GACCTGTTTT GCCAGGTCCT CGCCGGCGGT TTTTCGCTTC TTGGTCGTCA TAGTTCCTCG CGTGTCGATG GTCATCGACT TCGCCAAACC TGCCGCCTCC TGTTCGAGAC GACGCGAACG CTCCACGGCG GCCGATGGCG CGGGCAGGGC AGGGGGAGCC AGTTGCACGC TGTCGCGCTC GATCTTGGCC GTAGCTTGCT GGACCATCGA GCCGACGGAC TGGAAGGTTT CGCGGGGCGC ACGCATGACG GTGCGGCTTG CGATGGTTTC GGCATCCTCG GCGGAAAACC CCGCGTCGAT CAGTTCTTGC CTGTATGCCT TCCGGTCAAA CGTCCGATTC ATTCACCCTC CTTGCGGGAT TGCCCCGACT CACGCCGGGG CAATGTGCCC TTATTCCTGA TTTGACCCGC CTGGTGCCTT GGTGTCCAGA TAATCCACCT TATCGGCAAT GAAGTCGGTC CCGTAGACCG TCTGGCCGTC CTTCTCGTAC TTGGTATTCC GAATCTTGCC CTGCACGAAT ACCAGCGACC CCTTGCCCAA ATACTTGCCG TGGGCCTCGG CCTGAGAGCC AAAACACTTG ATGCGGAAGA AGTCGGTGCG CTCCTGCTTG TCGCCGGCAT CGTTGCGCCA CATCTAGGTA CTAAAACAAT TCATCCAGTA AAATATAATA TTTTATTTTC TCCCAATCAG GCTTGATCCC CAGTAAGTCA AAAAATAGCT CGACATACTG TTCTTCCCCG ATATCCTCCC TGATCGACCG GACGCAGAAG GCAATGTCAT ACCACTTGTC CGCCCTGCCG CTTCTCCCAA GATCAATAAA GCCACTTACT TTGCCATCTT TCACAAAGAT GTTGCTGTCT CCCAGGTCGC CGTGGGAAAA GACAAGTTCC TCTTCGGGCT TTTCCGTCTT TAAAAAATCA TACAGCTCGC GCGGATCTTT AAATGGAGTG TCTTCTTCCC AGTTTTCGCA ATCCACATCG GCCAGATCGT TATTCAGTAA GTAATCCAAT TCGGCTAAGC GGCTGTCTAA GCTATTCGTA TAGGGACAAT CCGATATGTC GATGGAGTGA AAGAGCCTGA TGCACTCCGC ATACAGCTCG ATAATCTTTT CAGGGCTTTG TTCATCTTCA TACTCTTCCG AGCAAAGGAC GCCATCGGCC TCACTCATGA GCAGATTGCT CCAGCCATCA TGCCGTTCAA AGTGCAGGAC CTTTGGAACA GGCAGCTTTC CTTCCAGCCA TAGCATCATG TCCTTTTCCC GTTCCACATC ATAGGTGGTC CCTTTATACC GGCTGTCCGT CATTTTTAAA TATAGGTTTT CATTTTCTCC CACCAGCTTA TATACCTTAG CAGGAGACAT TCCTTCCGTA TCTTTTACGC AGCGGTATTT TTCGATCAGT TTTTTCAATT CCGGTGATAT TCTCATTTTA GCCATTTATT ATTTCCTTCC TCTTTTCTAC AGTATTTAAA GATACCCCAA GAAGCTAATT ATAACAAGAC GAACTCCAAT TCACTGTTCC TTGCATTCTA AAACCTTAAA TACCAGAAAA CAGCTTTTTC AAAGTTGTTT TCAAAGTTGG CGTATAACAT AGTATCGACG GAGCCGATTT TGAAACCACA ATTATGGGTG ATGCTGCCAA CTTACTGATT TAGTGTATGA TGGTGTTTTT GAGGTGCTCC AGTGGCTTCT GTGTCTATCA GCTGTCCCTC CTGTTCAGCT ACTGACGGGG TGGTGCGTAA CGGCAAAAGC ACCGCCGGAC ATCAGCGCTA TCTCTGCTCT CACTGCCGTA AAACATGGCA ACTGCAGTTC ACTTACACCG CTTCTCAACC CGGTACGCAC CAGAAAATCA TTGATATGGC CATGAATGGC GTTGGATGCC GGGCAACAGC CCGCATTATG GGCGTTGGCC TCAACACGAT TTTACGTCAC TTAAAAAACT CAGGCCGCAG TCGGTAACCT CGCGCATACA GCCGGGCAGT GACGTCATCG TCTGCGCGGA AATGGACGAA CAGTGGGGCT ATGTCGGGGC TAAATCGCGC CAGCGCTGGC TGTTTTACGC GTATGACAGT CTCCGGAAGA CGGTTGTTGC GCACGTATTC GGTGAACGCA CTATGGCGAC GCTGGGGCGT CTTATGAGCC TGCTGTCACC CTTTGACGTG GTGATATGGA TGACGGATGG CTGGCCGCTG TATGAATCCC GCCTGAAGGG AAAGCTGCAC GTAATCAGCA AGCGATATAC GCAGCGAATT GAGCGGCATA ACCTGAATCT GAGGCAGCAC CTGGCACGGC TGGGACGGAA GTCGCTGTCG TTCTCAAAAT CGGTGGAGCT GCATGACAAA GTCATCGGGC ATTATCTGAA CATAAAACAC TATCAATAAG TTGGAGTCAT TACCCAATTA TGATAGAATT TACAAGCTAT AAGGTTATTG TCCTGGGTTT CAAGCATTAG TCCATGCAAG TTTTTATGCT TTGCCCATTC TATAGATATA TTGATAAGCG CGCTGCCTAT GCCTTGCCCC CTGAAATCCT TACATACGGC GATATCTTCT ATATAAAAGA TATATTATCT TATCAGTATT GTCAATATAT TCAAGGCAAT CTGCCTCCTC ATCCTCTTCA TCCTCTTCGT CTTGGTAGCT TTTTAAATAT GGCGCTTCAT AGAGTAATTC TGTAAAGGTC CAATTCTCGT TTTCATACCT CGGTATAATC TTACCTATCA CCTCAAATGG TTCGCTGGGT TTATCGCACC CCCGAACACG AGCACGGCAC CCGCGACCAC TATGCCAAGA ATGCCCAAGG TAAAAATTGC CGGCCCCGCC ATGAAGTCCG TGAATGCCCC GACGGCCGAA GTGAAGGGCA GGCCGCCACC CAGGCCGCCG CCCTCACTGC CCGGCACCTG GTCGCTGAAT GTCGATGCCA GCACCTGCGG CACGTCAATG CTTCCGGGCG TCGCGCTCGG GCTGATCGCC CATCCCGTTA CTGCCCCGAT CCCGGCAATG GCAAGGACTG CCAGCGCTGC CATTTTTGGG GTGAGGCCGT TCGCGGCCGA GGGGCGCAGC CCCTGGGGGG ATGGGAGGCC CGCGTTAGCG GGCCGGGAGG GTTCGAGAAG GGGGGGCACC CCCCTTCGGC GTGCGCGGTC ACGCGCACAG GGCGCAGCCC TGGTTAAAAA CAAGGTTTAT AAATATTGGT TTAAAAGCAG GTTAAAAGAC AGGTTAGCGG TGGCCGAAAA ACGGGCGGAA ACCCTTGCAA ATGCTGGATT TTCTGCCTGT GGACAGCCCC TCAAATGTCA ATAGGTGCGC CCCTCATCTG TCAGCACTCT GCCCCTCAAG TGTCAAGGAT CGCGCCCCTC ATCTGTCAGT AGTCGCGCCC CTCAAGTGTC AATACCGCAG GGCACTTATC CCCAGGCTTG TCCACATCAT CTGTGGGAAA CTCGCGTAAA ATCAGGCGTT TTCGCCGATT TGCGAGGCTG GCCAGCTCCA CGTCGCCGGC CGAAATCGAG CCTGCCCCTC ATCTGTCAAC GCCGCGCCGG GTGAGTCGGC CCCTCAAGTG TCAACGTCCG CCCCTCATCT GTCAGTGAGG GCCAAGTTTT CCGCGAGGTA TCCACAACGC CGGCGGCCGC GGTGTCTCGC ACACGGCTTC GACGGCGTTT CTGGCGCGTT TGCAGGGCCA TAGACGGCCG CCAGCCCAGC GGCGAGGGCA ACCAGCCCGG
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