pGWB12
$128.00
In stock
SKU
PL84171
100μg liquid plasmid(Instantly):$149
| name | pGWB12 | another name | apGWB12 |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Protein expression | Fragment type | ORF,GateWay |
| Fragment species | Empty vector | Prokaryotic resistance | Kan |
| Selective marker | Neo/G418,Hyg | Fluorescent labeling | |
| Promoter | 35s | Replicon | oriV |
| Competence | DB3.1 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGAGCGTCGC AAAGGCGCTC GGTCTTGCCT TGCTCGTCGG TGATGTACTT CACCAGCTCC GCGAAGTCGC TCTTCTTGAT GGAGCGCATG GGGACGTGCT TGGCAATCAC GCGCACCCCC CGGCCGTTTT AGCGGCTAAA AAAGTCATGG CTCTGCCCTC GGGCGGACCA CGCCCATCAT GACCTTGCCA AGCTCGTCCT GCTTCTCTTC GATCTTCGCC AGCAGGGCGA GGATCGTGGC ATCACCGAAC CGCGCCGTGC GCGGGTCGTC GGTGAGCCAG AGTTTCAGCA GGCCGCCCAG GCGGCCCAGG TCGCCATTGA TGCGGGCCAG CTCGCGGACG TGCTCATAGT CCACGACGCC CGTGATTTTG TAGCCCTGGC CGACGGCCAG CAGGTAGGCC GACAGGCTCA TGCCGGCCGC CGCCGCCTTT TCCTCAATCG CTCTTCGTTC GTCTGGAAGG CAGTACACCT TGATAGGTGG GCTGCCCTTC CTGGTTGGCT TGGTTTCATC AGCCATCCGC TTGCCCTCAT CTGTTACGCC GGCGGTAGCC GGCCAGCCTC GCAGAGCAGG ATTCCCGTTG AGCACCGCCA GGTGCGAATA AGGGACAGTG AAGAAGGAAC ACCCGCTCGC GGGTGGGCCT ACTTCACCTA TCCTGCCCGG CTGACGCCGT TGGATACACC AAGGAAAGTC TACACGAACC CTTTGGCAAA ATCCTGTATA TCGTGCGAAA AAGGATGGAT ATACCGAAAA AATCGCTATA ATGACCCCGA AGCAGGGTTA TGCAGCGGAA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCAGA AGGCCGCCAG AGAGGCCGAG CGCGGCCGTG AGGCTTGGAC GCTAGGGCAG GGCATGAAAA AGCCCGTAGC GGGCTGCTAC GGGCGTCTGA CGCGGTGGAA AGGGGGAGGG GATGTTGTCT ACATGGCTCT GCTGTAGTGA GTGGGTTGCG CTCCGGCAGC GGTCCTGATC AATCGTCACC CTTTCTCGGT CCTTCAACGT TCCTGACAAC GAGCCTCCTT TTCGCCAATC CATCGACAAT CACCGCGAGT CCCTGCTCGA ACGCTGCGTC CGGACCGGCT TCGTCGAAGG CGTCTATCGC GGCCCGCAAC AGCGGCGAGA GCGGAGCCTG TTCAACGGTG CCGCCGCGCT CGCCGGCATC GCTGTCGCCG GCCTGCTCCT CAAGCACGGC CCCAACAGTG AAGTAGCTGA TTGTCATCAG CGCATTGACG GCGTCCCCGG CCGAAAAACC CGCCTCGCAG AGGAAGCGAA GCTGCGCGTC GGCCGTTTCC ATCTGCGGTG CGCCCGGTCG CGTGCCGGCA TGGATGCGCG CGCCATCGCG GTAGGCGAGC AGCGCCTGCC TGAAGCTGCG GGCATTCCCG ATCAGAAATG AGCGCCAGTC GTCGTCGGCT CTCGGCACCG AATGCGTATG ATTCTCCGCC AGCATGGCTT CGGCCAGTGC GTCGAGCAGC GCCCGCTTGT TCCTGAAGTG CCAGTAAAGC GCCGGCTGCT GAACCCCCAA CCGTTCCGCC AGTTTGCGTG TCGTCAGACC GTCTACGCCG ACCTCGTTCA ACAGGTCCAG GGCGGCACGG ATCACTGTAT TCGGCTGCAA CTTTGTCATG CTTGACACTT TATCACTGAT AAACATAATA TGTCCACCAA CTTATCAGTG ATAAAGAATC CGCGCGTTCA ATCGGACCAG CGGAGGCTGG TCCGGAGGCC AGACGTGAAA CCCAACATAC CCCTGATCGT AATTCTGAGC ACTGTCGCGC TCGACGCTGT CGGCATCGGC CTGATTATGC CGGTGCTGCC GGGCCTCCTG CGCGATCTGG TTCACTCGAA CGACGTCACC GCCCACTATG GCATTCTGCT GGCGCTGTAT GCGTTGGTGC AATTTGCCTG CGCACCTGTG CTGGGCGCGC TGTCGGATCG TTTCGGGCGG CGGCCAATCT TGCTCGTCTC GCTGGCCGGC GCCAGATCTG GGGAACCCTG TGGTTGGCAT GCACATACAA ATGGACGAAC GGATAAACCT TTTCACGCCC TTTTAAATAT CCGATTATTC TAATAAACGC TCTTTTCTCT TAGGTTTACC CGCCAATATA TCCTGTCAAA CACTGATAGT TTAAACTGAA GGCGGGAAAC GACAATCTGA TCATGAGCGG AGAATTAAGG GAGTCACGTT ATGACCCCCG CCGATGACGC GGGACAAGCC GTTTTACGTT TGGAACTGAC AGAACCGCAA CGTTGAAGGA GCCACTCAGC CGCGGGTTTC TGGAGTTTAA TGAGCTAAGC ACATACGTCA GAAACCATTA TTGCGCGTTC AAAAGTCGCC TAAGGTCACT ATCAGCTAGC AAATATTTCT TGTCAAAAAT GCTCCACTGA CGTTCCATAA ATTCCCCTCG GTATCCAATT AGAGTCTCAT ATTCACTCTC AATCCAAATA ATCTGCACCG GATCTGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GATGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACGGGATCTC TGCGGAACAG GCGGTCGAAG GTGCCGATAT CATTACGACA GCAACGGCCG ACAAGCACAA CGCCACGATC CTGAGCGACA ATATGATCGG GCCCGGCGTC CACATCAACG GCGTCGGCGG CGACTGCCCA GGCAAGACCG AGATGCACCG CGATATCTTG CTGCGTTCGG ATATTTTCGT GGAGTTCCCG CCACAGACCC GGATGATCCC CGATCGTTCA AACATTTGGC AATAAAGTTT CTTAAGATTG AATCCTGTTG CCGGTCTTGC GATGATTATC ATATAATTTC TGTTGAATTA CGTTAAGCAT GTAATAATTA ACATGTAATG CATGACGTTA TTTATGAGAT GGGTTTTTAT GATTAGAGTC CCGCAATTAT ACATTTAATA CGCGATAGAA AACAAAATAT AGCGCGCAAA CTAGGATAAA TTATCGCGCG CGGTGTCATC TATGTTACTA GATCGGGCCT CCTGTCAATG CTGGCGGCGG CTCTGGTGGT GGTTCTGGTG GCGGCTCTGA GGGTGGTGGC TCTGAGGGTG GCGGTTCTGA GGGTGGCGGC TCTGAGGGAG GCGGTTCCGG TGGTGGCTCT GGTTCCGGTG ATTTTGATTA TGAAAAGATG GCAAACGCTA ATAAGGGGGC TATGACCGAA AATGCCGATG AAAACGCGCT ACAGTCTGAC GCTAAAGGCA AACTTGATTC TGTCGCTACT GATTACGGTG CTGCTATCGA TGGTTTCATT GGTGACGTTT CCGGCCTTGC TAATGGTAAT GGTGCTACTG GTGATTTTGC TGGCTCTAAT TCCCAAATGG CTCAAGTCGG TGACGGTGAT AATTCACCTT TAATGAATAA TTTCCGTCAA TATTTACCTT CCCTCCCTCA ATCGGTTGAA TGTCGCCCTT TTGTCTTTGG CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGCCA AGCTTGCATG CCTGCAGGTC CCCAGATTAG CCTTTTCAAT TTCAGAAAGA ATGCTAACCC ACAGATGGTT AGAGAGGCTT ACGCAGCAGG TCTCATCAAG ACGATCTACC CGAGCAATAA TCTCCAGGAA ATCAAATACC TTCCCAAGAA GGTTAAAGAT GCAGTCAAAA GATTCAGGAC TAACTGCATC AAGAACACAG AGAAAGATAT ATTTCTCAAG ATCAGAAGTA CTATTCCAGT ATGGACGATT CAAGGCTTGC TTCACAAACC AAGGCAAGTA ATAGAGATTG GAGTCTCTAA AAAGGTAGTT CCCACTGAAT CAAAGGCCAT GGAGTCAAAG ATTCAAATAG AGGACCTAAC AGAACTCGCC GTAAAGACTG GCGAACAGTT CATACAGAGT CTCTTACGAC TCAATGACAA GAAGAAAATC TTCGTCAACA TGGTGGAGCA CGACACACTT GTCTACTCCA AAAATATCAA AGATACAGTC TCAGAAGACC AAAGGGCAAT TGAGACTTTT CAACAAAGGG TAATATCCGG AAACCTCCTC GGATTCCATT GCCCAGCTAT CTGTCACTTT ATTGTGAAGA TAGTGGAAAA GGAAGGTGGC TCCTACAAAT GCCATCATTG CGATAAAGGA AAGGCCATCG TTGAAGATGC CTCTGCCGAC AGTGGTCCCA AAGATGGACC CCCACCCACG AGGAGCATCG TGGAAAAAGA AGACGTTCCA ACCACGTCTT CAAAGCAAGT GGATTGATGT GATATCTCCA CTGACGTAAG GGATGACGCA CAATCCCACT ATCCTTCGCA AGACCCTTCC TCTATATAAG GAAGTTCATT TCATTTGGAG AGAACACGGG GGACTCTAGA ATGAGCGACT ACAAGGATGA CGATGACAAG GCTGTTATCA CAAGTTTGTA CAAAAAAGCT GAACGAGAAA CGTAAAATGA TATAAATATC AATATATTAA ATTAGATTTT GCATAAAAAA CAGACTACAT AATACTGTAA AACACAACAT ATCCAGTCAC TATGGCGGCC CCTCGAGGGG CCGCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATAAT GTGTGGATTT TGAGTTAGGA TCCGGCGAGA TTTTCAGGAG CTAAGGAAGC TAAAATGGAG AAAAAAATCA CTGGATATAC CACCGTTGAT ATATCCCAAT GGCATCGTAA AGAACATTTT GAGGCATTTC AGTCAGTTGC TCAATGTACC TATAACCAGA CCGTTCAGCT GGATATTACG GCCTTTTTAA AGACCGTAAA GAAAAATAAG CACAAGTTTT ATCCGGCCTT TATTCACATT CTTGCCCGCC TGATGAATGC TCATCCGGAA TTCCGTATGG CAATGAAAGA CGGTGAGCTG GTGATATGGG ATAGTGTTCA CCCTTGTTAC ACCGTTTTCC ATGAGCAAAC TGAAACGTTT TCATCGCTCT GGAGTGAATA CCACGACGAT TTCCGGCAGT TTCTACACAT ATATTCGCAA GATGTGGCGT GTTACGGTGA AAACCTGGCC TATTTCCCTA AAGGGTTTAT TGAGAATATG TTTTTCGTCT CAGCCAATCC CTGGGTGAGT TTCACCAGTT TTGATTTAAA CGTGGCCAAT ATGGACAACT TCTTCGCCCC CGTTTTCACC ATGGGCAAAT ATTATACGCA AGGCGACAAG GTGCTGATGC CGCTGGCGAT TCAGGTTCAT CATGCCGTCT GTGATGGCTT CCATGTCGGC AGAATGCTTA ATGAATTACA ACAGTACTGC GATGAGTGGC AGGGCGGGGC GTAAACGCGT GGATCCGGCT TACTAAAAGC CAGATAACAG TATGCGTATT TGCGCGCTGA TTTTTGCGGT ATAAGAATAT ATACTGATAT GTATACCCGA AGTATGTCAA AAAGAGGTGT GCTATGAAGC AGCGTATTAC AGTGACAGTT GACAGCGACA GCTATCAGTT GCTCAAGGCA TATATGATGT CAATATCTCC GGTCTGGTAA GCACAACCAT GCAGAATGAA GCCCGTCGTC TGCGTGCCGA ACGCTGGAAA GCGGAAAATC AGGAAGGGAT GGCTGAGGTC GCCCGGTTTA TTGAAATGAA CGGCTCTTTT GCTGACGAGA ACAGGGACTG GTGAAATGCA GTTTAAGGTT TACACCTATA AAAGAGAGAG CCGTTATCGT CTGTTTGTGG ATGTACAGAG TGATATTATT GACACGCCCG GGCGACGGAT GGTGATCCCC CTGGCCAGTG CACGTCTGCT GTCAGATAAA GTCTCCCGTG AACTTTACCC GGTGGTGCAT ATCGGGGATG AAAGCTGGCG CATGATGACC ACCGATATGG CCAGTGTGCC GGTCTCCGTT ATCGGGGAAG AAGTGGCTGA TCTCAGCCAC CGCGAAAATG ACATCAAAAA CGCCATTAAC CTGATGTTCT GGGGAATATA AATGTCAGGC TCCCTTATAC ACAGCCAGTC TGCAGGTCGA CCATAGTGAC TGGATATGTT GTGTTTTACA GTATTATGTA GTCTGTTTTT TATGCAAAAT CTAATTTAAT ATATTGATAT TTATATCATT TTACGTTTCT CGTTCAGCTT TCTTGTACAA AGTGGTGATA ACCTAGAGCT CGAATTTCCC CGATCGTTCA AACATTTGGC AATAAAGTTT CTTAAGATTG AATCCTGTTG CCGGTCTTGC GATGATTATC ATATAATTTC TGTTGAATTA CGTTAAGCAT GTAATAATTA ACATGTAATG CATGACGTTA TTTATGAGAT GGGTTTTTAT GATTAGAGTC CCGCAATTAT ACATTTAATA CGCGATAGAA AACAAAATAT AGCGCGCAAA CTAGGATAAA TTATCGCGCG CGGTGTCATC TATGTTACTA GATCGGGAAT TAGCTTCATC AACGCAAGAC ATGCGCACGA CCGTCTGACA GGAGAGGAAT TTCCGACGAG CACAGAAAGG ACTTGCTCTT GGACGTAGGC CTATTTCTCA GGCACATGTA TCAAGTGTTC GGACGTGGGT TTTCGATGGT GTATCAGCCG CCGCCAACTG GGAGATGAGG AGGCTTTCTT GGGGGGCAGT CAGCAGTTCA TTTCACAAGA CAGAGGAACT TGTAAGGAGA TGCACTGATT TATCTTGGCG CAAACCAGCA GGACGAATTA GTGGGAATAG CCCGCGAATA TCTAAGTTAT GCCTGTCGGC ATGAGCAGAA ACTTCCAATT CGAAACAGTT TGGAGAGGTT GTTTTTGGGC ATACCTTTTG TTAGTCAGCC TCTCGATTGC TCATCGTCAT TACACAGTAC CGAAGTTTGA TCGATCTAGT AACATAGATG ACACCGCGCG CGATAATTTA TCCTAGTTTG CGCGCTATAT TTTGTTTTCT ATCGCGTATT AAATGTATAA TTGCGGGACT CTAATCATAA AAACCCATCT CATAAATAAC GTCATGCATT ACATGTTAAT TATTACATGC TTAACGTAAT TCAACAGAAA TTATATGATA ATCATCGCAA GACCGGCAAC AGGATTCAAT CTTAAGAAAC TTTATTGCCA AATGTTTGAA CGATCTGCTT CGACGCACTC CTTCTTTACT CCACCATCTC GTCCTTATTG AAAACGTGGG TAGCACCAAA ACGAATCAAG TCGCTGGAAC TGAAGTTACC AATCACGCTG GATGATTTGC CAGTTGGATT AATCTTGCCT TTCCCCGCAT GAATAATATT GATGAATGCA TGCGTGAGGG GTATTTCGAT TTTGGCAATA GCTGCAATTG CCGCGACATC CTCCAACGAG CATAATTCTT CAGAAAAATA GCGATGTTCC ATGTTGTCAG GGCATGCATG ATGCACGTTA TGAGGTGACG GTGCTAGGCA GTATTCCCTC AAAGTTTCAT AGTCAGTATC ATATTCATCA TTGCATTCCT GCAAGAGAGA ATTGAGACGC AATCCACACG CTGCGGCAAC CTTCCGGCGT TCGTGGTCTA TTTGCTCTTG GACGTTGCAA ACGTAAGTGT TGGATCCCGG TCGGCATCTA CTCTATTCCT TTGCCCTCGG ACGAGTGCTG GGGCGTCGGT TTCCACTATC GGCGAGTACT TCTACACAGC CATCGGTCCA GACGGCCGCG CTTCTGCGGG CGATTTGTGT ACGCCCGACA GTCCCGGCTC CGGATCGGAC GATTGCGTCG CATCGACCCT GCGCCCAAGC TGCATCATCG AAATTGCCGT CAACCAAGCT CTGATAGAGT TGGTCAAGAC CAATGCGGAG CATATACGCC CGGAGCCGCG GCGATCCTGC AAGCTCCGGA TGCCTCCGCT CGAAGTAGCG CGTCTGCTGC TCCATACAAG CCAACCACGG CCTCCAGAAG AAGATGTTGG CGACCTCGTA TTGGGAATCC CCGAACATCG CCTCGCTCCA GTCAATGACC GCTGTTATGC GGCCATTGTC CGTCAGGACA TTGTTGGAGC CGAAATCCGC GTGCACGAGG TGCCGGACTT CGGGGCAGTC CTCGGCCCAA AGCATCAGCT CATCGAGAGC CTGCGCGACG GACGCACTGA CGGTGTCGTC CATCACAGTT TGCCAGTGAT ACACATGGGG ATCAGCAATC GCGCATATGA AATCACGCCA TGTAGTGTAT TGACCGATTC CTTGCGGTCC GAATGGGCCG AACCCGCTCG TCTGGCTAAG ATCGGCCGCA GCGATCGCAT CCATGGCCTC CGCGACCGGC TGCAGAACAG CGGGCAGTTC GGTTTCAGGC AGGTCTTGCA ACGTGACACC CTGTGCACGG CGGGAGATGC AATAGGTCAG GCTCTCGCTG AATTCCCCAA TGTCAAGCAC TTCCGGAATC GGGAGCGCGG CCGATGCAAA GTGCCGATAA ACATAACGAT CTTTGTAGAA ACCATCGGCG CAGCTATTTA CCCGCAGGAC ATATCCACGC CCTCCTACAT CGAAGCTGAA AGCACGAGAT TCTTCGCCCT CCGAGAGCTG CATCAGGTCG GAGACGCTGT CGAACTTTTC GATCAGAAAC TTCTCGACAG ACGTCGCGGT GAGTTCAGGC TTTTTCATAT CGGGGTCGTC CTCTCCAAAT GAAATGAACT TCCTTATATA GAGGAAGGGT CTTGCGAAGG ATAGTGGGAT TGTGCGTCAT CCCTTACGTC AGTGGAGATA TCACATCAAT CCACTTGCTT TGAAGACGTG GTTGGAACGT CTTCTTTTTC CACGATGCTC CTCGTGGGTG GGGGTCCATC TTTGGGACCA CTGTCGGCAG AGGCATCTTG AACGATAGCC TTTCCTTTAT CGCAATGATG GCATTTGTAG GTGCCACCTT CCTTTTCTAC TGTCCTTTTG ATGAAGTGAC AGATAGCTGG GCAATGGAAT CCGAGGAGGT TTCCCGATAT TACCCTTTGT TGAAAAGTCT CAATAGCCCT TTGGTCTTCT GAGACTGTAT CTTTGATATT CTTGGAGTAG ACGAGAGTGT CGTGCTCCAC CATGTTGACG GATCTCTAGG ACGCGTCCTA GAAGCTAATT CACTGGCCGT CGTTTTACAA CGTCGTGACT GGGAAAACCC TGGCGTTACC CAACTTAATC GCCTTGCAGC ACATCCCCCT TTCGCCAGCT GGCGTAATAG CGAAGAGGCC CGCACCGATC GCCCTTCCCA ACAGTTGCGC AGCCTGAATG GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTTGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGGCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GAACCACCAT CAAACAGGAT TTTCGCCTGC TGGGGCAAAC CAGCGTGGAC CGCTTGCTGC AACTCTCTCA GGGCCAGGCG GTGAAGGGCA ATCAGCTGTT GCCCGTCTCA CTGGTGAAAA GAAAAACCAC CCCAGTACAT TAAAAACGTC CGCAATGTGT TATTAAGTTG TCTAAGCGTC AATTTGTTTA CACCACAATA TATCCTGCCA CCAGCCAGCC AACAGCTCCC CGACCGGCAG CTCGGCACAA AATCACCACT CGATACAGGC AGCCCATCAG TCCGGGACGG CGTCAGCGGG AGAGCCGTTG TAAGGCGGCA GACTTTGCTC ATGTTACCGA TGCTATTCGG AAGAACGGCA ACTAAGCTGC CGGGTTTGAA ACACGGATGA TCTCGCGGAG GGTAGCATGT TGATTGTAAC GATGACAGAG CGTTGCTGCC TGTGATCAAA TATCATCTCC CTCGCAGAGA TCCGAATTAT CAGCCTTCTT ATTCATTTCT CGCTTAACCG TGACAGGCTG TCGATCTTGA GAACTATGCC GACATAATAG GAAATCGCTG GATAAAGCCG CTGAGGAAGC TGAGTGGCGC TATTTCTTTA GAAGTGAACG TTGACGATAT CAACTCCCCT ATCCATTGCT CACCGAATGG TACAGGTCGG GGACCCGAAG TTCCGACTGT CGGCCTGATG CATCCCCGGC TGATCGACCC CAGATCTGGG GCTGAGAAAG CCCAGTAAGG AAACAACTGT AGGTTCGAGT CGCGAGATCC CCCGGAACCA AAGGAAGTAG GTTAAACCCG CTCCGATCAG GCCGAGCCAC GCCAGGCCGA GAACATTGGT TCCTGTAGGC ATCGGGATTG GCGGATCAAA CACTAAAGCT ACTGGAACGA GCAGAAGTCC TCCGGCCGCC AGTTGCCAGG CGGTAAAGGT GAGCAGAGGC ACGGGAGGTT GCCACTTGCG GGTCAGCACG GTTCCGAACG CCATGGAAAC CGCCCCCGCC AGGCCCGCTG CGACGCCGAC AGGATCTAGC GCTGCGTTTG GTGTCAACAC CAACAGCGCC ACGCCCGCAG TTCCGCAAAT AGCCCCCAGG ACCGCCATCA ATCGTATCGG GCTACCTAGC AGAGCGGCAG AGATGAACAC GACCATCAGC GGCTGCACAG CGCCTACCGT CGCCGCGACC CCGCCCGGCA GGCGGTAGAC CGAAATAAAC AACAAGCTCC AGAATAGCGA AATATTAAGT GCGCCGAGGA TGAAGATGCG CATCCACCAG ATTCCCGTTG GAATCTGTCG GACGATCATC ACGAGCAATA AACCCGCCGG CAACGCCCGC AGCAGCATAC CGGCGACCCC TCGGCCTCGC TGTTCGGGCT CCACGAAAAC GCCGGACAGA TGCGCCTTGT GAGCGTCCTT GGGGCCGTCC TCCTGTTTGA AGACCGACAG CCCAATGATC TCGCCGTCGA TGTAGGCGCC GAATGCCACG GCATCTCGCA ACCGTTCAGC GAACGCCTCC ATGGGCTTTT TCTCCTCGTG CTCGTAAACG GACCCGAACA TCTCTGGAGC TTTCTTCAGG GCCGACAATC GGATCTCGCG GAAATCCTGC ACGTCGGCCG CTCCAAGCCG TCGAATCTGA GCCTTAATCA CAATTGTCAA TTTTAATCCT CTGTTTATCG GCAGTTCGTA GAGCGCGCCG TGCGTCCCGA GCGATACTGA GCGAAGCAAG TGCGTCGAGC AGTGCCCGCT TGTTCCTGAA ATGCCAGTAA AGCGCTGGCT GCTGAACCCC CAGCCGGAAC TGACCCCACA AGGCCCTAGC GTTTGCAATG CACCAGGTCA TCATTGACCC AGGCGTGTTC CACCAGGCCG CTGCCTCGCA ACTCTTCGCA GGCTTCGCCG ACCTGCTCGC GCCACTTCTT CACGCGGGTG GAATCCGATC CGCACATGAG GCGGAAGGTT TCCAGCTTGA GCGGGTACGG CTCCCGGTGC GAGCTGAAAT AGTCGAACAT CCGTCGGGCC GTCGGCGACA GCTTGCGGTA CTTCTCCCAT ATGAATTTCG TGTAGTGGTC GCCAGCAAAC AGCACGACGA TTTCCTCGTC GATCAGGACC TGGCAACGGG ACGTTTTCTT GCCACGGTCC AGGACGCGGA AGCGGTGCAG CAGCGACACC GATTCCAGGT GCCCAACGCG GTCGGACGTG AAGCCCATCG CCGTCGCCTG TAGGCGCGAC AGGCATTCCT CGGCCTTCGT GTAATACCGG CCATTGATCG ACCAGCCCAG GTCCTGGCAA AGCTCGTAGA ACGTGAAGGT GATCGGCTCG CCGATAGGGG TGCGCTTCGC GTACTCCAAC ACCTGCTGCC ACACCAGTTC GTCATCGTCG GCCCGCAGCT CGACGCCGGT GTAGGTGATC TTCACGTCCT TGTTGACGTG GAAAATGACC TTGTTTTGCA GCGCCTCGCG CGGGATTTTC TTGTTGCGCG TGGTGAACAG GGCAGAGCGG GCCGTGTCGT TTGGCATCGC TCGCATCGTG TCCGGCCACG GCGCAATATC GAACAAGGAA AGCTGCATTT CCTTGATCTG CTGCTTCGTG TGTTTCAGCA ACGCGGCCTG CTTGGCCTCG CTGACCTGTT TTGCCAGGTC CTCGCCGGCG GTTTTTCGCT TCTTGGTCGT CATAGTTCCT CGCGTGTCGA TGGTCATCGA CTTCGCCAAA CCTGCCGCCT CCTGTTCGAG ACGACGCGAA CGCTCCACGG CGGCCGATGG CGCGGGCAGG GCAGGGGGAG CCAGTTGCAC GCTGTCGCGC TCGATCTTGG CCGTAGCTTG CTGGACCATC GAGCCGACGG ACTGGAAGGT TTCGCGGGGC GCACGCATGA CGGTGCGGCT TGCGATGGTT TCGGCATCCT CGGCGGAAAA CCCCGCGTCG ATCAGTTCTT GCCTGTATGC CTTCCGGTCA AACGTCCGAT TCATTCACCC TCCTTGCGGG ATTGCCCCGA CTCACGCCGG GGCAATGTGC CCTTATTCCT GATTTGACCC GCCTGGTGCC TTGGTGTCCA GATAATCCAC CTTATCGGCA ATGAAGTCGG TCCCGTAGAC CGTCTGGCCG TCCTTCTCGT ACTTGGTATT CCGAATCTTG CCCTGCACGA ATACCAGCGA CCCCTTGCCC AAATACTTGC CGTGGGCCTC GGCCTGAGAG CCAAAACACT TGATGCGGAA GAAGTCGGTG CGCTCCTGCT TGTCGCCGGC ATCGTTGCGC CACATCTAGG TACTAAAACA ATTCATCCAG TAAAATATAA TATTTTATTT TCTCCCAATC AGGCTTGATC CCCAGTAAGT CAAAAAATAG CTCGACATAC TGTTCTTCCC CGATATCCTC CCTGATCGAC CGGACGCAGA AGGCAATGTC ATACCACTTG TCCGCCCTGC CGCTTCTCCC AAGATCAATA AAGCCACTTA CTTTGCCATC TTTCACAAAG ATGTTGCTGT CTCCCAGGTC GCCGTGGGAA AAGACAAGTT CCTCTTCGGG CTTTTCCGTC TTTAAAAAAT CATACAGCTC GCGCGGATCT TTAAATGGAG TGTCTTCTTC CCAGTTTTCG CAATCCACAT CGGCCAGATC GTTATTCAGT AAGTAATCCA ATTCGGCTAA GCGGCTGTCT AAGCTATTCG TATAGGGACA ATCCGATATG TCGATGGAGT GAAAGAGCCT GATGCACTCC GCATACAGCT CGATAATCTT TTCAGGGCTT TGTTCATCTT CATACTCTTC CGAGCAAAGG ACGCCATCGG CCTCACTCAT GAGCAGATTG CTCCAGCCAT CATGCCGTTC AAAGTGCAGG ACCTTTGGAA CAGGCAGCTT TCCTTCCAGC CATAGCATCA TGTCCTTTTC CCGTTCCACA TCATAGGTGG TCCCTTTATA CCGGCTGTCC GTCATTTTTA AATATAGGTT TTCATTTTCT CCCACCAGCT TATATACCTT AGCAGGAGAC ATTCCTTCCG TATCTTTTAC GCAGCGGTAT TTTTCGATCA GTTTTTTCAA TTCCGGTGAT ATTCTCATTT TAGCCATTTA TTATTTCCTT CCTCTTTTCT ACAGTATTTA AAGATACCCC AAGAAGCTAA TTATAACAAG ACGAACTCCA ATTCACTGTT CCTTGCATTC TAAAACCTTA AATACCAGAA AACAGCTTTT TCAAAGTTGT TTTCAAAGTT GGCGTATAAC ATAGTATCGA CGGAGCCGAT TTTGAAACCA CAATTATGGG TGATGCTGCC AACTTACTGA TTTAGTGTAT GATGGTGTTT TTGAGGTGCT CCAGTGGCTT CTGTGTCTAT CAGCTGTCCC TCCTGTTCAG CTACTGACGG GGTGGTGCGT AACGGCAAAA GCACCGCCGG ACATCAGCGC TATCTCTGCT CTCACTGCCG TAAAACATGG CAACTGCAGT TCACTTACAC CGCTTCTCAA CCCGGTACGC ACCAGAAAAT CATTGATATG GCCATGAATG GCGTTGGATG CCGGGCAACA GCCCGCATTA TGGGCGTTGG CCTCAACACG ATTTTACGTC ACTTAAAAAA CTCAGGCCGC AGTCGGTAAC CTCGCGCATA CAGCCGGGCA GTGACGTCAT CGTCTGCGCG GAAATGGACG AACAGTGGGG CTATGTCGGG GCTAAATCGC GCCAGCGCTG GCTGTTTTAC GCGTATGACA GTCTCCGGAA GACGGTTGTT GCGCACGTAT TCGGTGAACG CACTATGGCG ACGCTGGGGC GTCTTATGAG CCTGCTGTCA CCCTTTGACG TGGTGATATG GATGACGGAT GGCTGGCCGC TGTATGAATC CCGCCTGAAG GGAAAGCTGC ACGTAATCAG CAAGCGATAT ACGCAGCGAA TTGAGCGGCA TAACCTGAAT CTGAGGCAGC ACCTGGCACG GCTGGGACGG AAGTCGCTGT CGTTCTCAAA ATCGGTGGAG CTGCATGACA AAGTCATCGG GCATTATCTG AACATAAAAC ACTATCAATA AGTTGGAGTC ATTACCCAAT TATGATAGAA TTTACAAGCT ATAAGGTTAT TGTCCTGGGT TTCAAGCATT AGTCCATGCA AGTTTTTATG CTTTGCCCAT TCTATAGATA TATTGATAAG CGCGCTGCCT ATGCCTTGCC CCCTGAAATC CTTACATACG GCGATATCTT CTATATAAAA GATATATTAT CTTATCAGTA TTGTCAATAT ATTCAAGGCA ATCTGCCTCC TCATCCTCTT CATCCTCTTC GTCTTGGTAG CTTTTTAAAT ATGGCGCTTC ATAGAGTAAT TCTGTAAAGG TCCAATTCTC GTTTTCATAC CTCGGTATAA TCTTACCTAT CACCTCAAAT GGTTCGCTGG GTTTATCGCA CCCCCGAACA CGAGCACGGC ACCCGCGACC ACTATGCCAA GAATGCCCAA GGTAAAAATT GCCGGCCCCG CCATGAAGTC CGTGAATGCC CCGACGGCCG AAGTGAAGGG CAGGCCGCCA CCCAGGCCGC CGCCCTCACT GCCCGGCACC TGGTCGCTGA ATGTCGATGC CAGCACCTGC GGCACGTCAA TGCTTCCGGG CGTCGCGCTC GGGCTGATCG CCCATCCCGT TACTGCCCCG ATCCCGGCAA TGGCAAGGAC TGCCAGCGCT GCCATTTTTG GGGTGAGGCC GTTCGCGGCC GAGGGGCGCA GCCCCTGGGG GGATGGGAGG CCCGCGTTAG CGGGCCGGGA GGGTTCGAGA AGGGGGGGCA CCCCCCTTCG GCGTGCGCGG TCACGCGCAC AGGGCGCAGC CCTGGTTAAA AACAAGGTTT ATAAATATTG GTTTAAAAGC AGGTTAAAAG ACAGGTTAGC GGTGGCCGAA AAACGGGCGG AAACCCTTGC AAATGCTGGA TTTTCTGCCT GTGGACAGCC CCTCAAATGT CAATAGGTGC GCCCCTCATC TGTCAGCACT CTGCCCCTCA AGTGTCAAGG ATCGCGCCCC TCATCTGTCA GTAGTCGCGC CCCTCAAGTG TCAATACCGC AGGGCACTTA TCCCCAGGCT TGTCCACATC ATCTGTGGGA AACTCGCGTA AAATCAGGCG TTTTCGCCGA TTTGCGAGGC TGGCCAGCTC CACGTCGCCG GCCGAAATCG AGCCTGCCCC TCATCTGTCA ACGCCGCGCC GGGTGAGTCG GCCCCTCAAG TGTCAACGTC CGCCCCTCAT CTGTCAGTGA GGGCCAAGTT TTCCGCGAGG TATCCACAAC GCCGGCGGCC GCGGTGTCTC GCACACGGCT TCGACGGCGT TTCTGGCGCG TTTGCAGGGC CATAGACGGC CGCCAGCCCA GCGGCGAGGG CAACCAGCCC GG
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