pCMV-BKCA-FLAG
$128.00
In stock
SKU
PL84168
AF118141.1
100μg liquid plasmid(Instantly):$149
| name | pCMV-BKCA-FLAG | another name | apCMV-BKCA-FLAG |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GAGTTCGAGC TTGCATGCCT GCAGGTCGTT ACATAACTTA CGGTAAATGG CCCGCCTGGC TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA CGTATGTTCC CATAGTAACG CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC TGCCCACTTG GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA TGACGGTAAA TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT ATATAAGCAG AGCTCGTTTA GTGAACCGTC AGATCGCCTG GAGACGCCAT CCACGCTGTT TTGACCTCCA TAGAAGACAC CGGGACCGAT CCAGCCTCCG GACTCTAGAG GATCCGGTAC TAGAGGAACT GAAAAACCAG AAAGTTAACT GGTAAGTTTA GTCTTTTTGT CTTTTATTTC AGGTCCCGGA TCCGGTGGTG GTGCAAATCA AAGAACTGCT CCTCAGTGGA TGTTGCCTTT ACTTCTAGGC CTGTACGGAA GTGTTACTTC TGCTCTAAAA GCTGCGGAAT TGTACCCGCG GGCCCAGGCC CGAATTCGGT CGACGCCACC ATGGATGCGC TCATCATCCC GGTGACCATG GAGGTGCCGT GCGACAGCCG GGGCCAACGC ATGTGGTGGG CTTTCCTGGC CTCCTCCATG GTGACTTTCT TCGGGGGCCT CTTCATCATC TTGCTCTGGC GGACGCTCAA GTACCTGTGG ACCGTGTGCT GCCACTGCGG GGGCAAGACG AAGGAGGCCC AGAAGATTAA CAATGGCTCA AGCCAGGCGG ATGGCACTCT CAAACCAGTG GATGAAAAAG AGGAGGCAGT GGCCGCCGAG GTCGGCTGGA TGACCTCCGT GAAGGACTGG GCGGGGGTGA TGATATCCGC CCAGACACTG ACTGGCAGAG TCCTGGTTGT CTTAGTCTTT GCTCTCAGCA TCGGTGCACT TGTAATATAC TTCATAGATT CATCAAACCC AATAGAATCC TGCCAGAATT TCTACAAAGA TTTCACATTA CAGATCGACA TGGCTTTCAA CGTGTTCTTC CTTCTCTACT TTGGCTTGCG GTTTATTGCA GCCAACGATA AATTGTGGTT CTGGCTGGAA GTGAACTCTG TAGTGGATTT CTTCACGGTG CCCCCCGTGT TTGTGTCTGT GTACTTAAAC AGAAGTTGGC TTGGTTTGAG ATTTTTAAGA GCTCTGAGAC TGATACAGTT TTCAGAAATT TTGCAGTTTC TGAATATTCT TAAAACAAGT AATTCCATCA AGCTGGTGAA TCTGCTCTCC ATATTTATCA GCACGTGGCT GACTGCAGCC GGGTTCATCC ATTTGGTGGA GAATTCAGGG GACCCATGGG AAAATTTCCA AAACAACCAG GCTCTCACCT ACTGGGAATG TGTCTATTTA CTCATGGTCA CAATGTCCAC CGTTGGTTAT GGGGATGTTT ATGCAAAAAC CACACTTGGG CGCCTCTTCA TGGTCTTCTT CATCCTCGGG GGACTGGCCA TGTTTGCCAG CTACGTCCCT GAAATCATAG AGTTAATAGG AAACCGCAAG AAATACGGGG GCTCCTATAG TGCGGTTAGT GGAAGAAAGC ACATTGTGGT CTGCGGACAC ATCACTCTGG AGAGTGTTTC CAACTTCCTG AAGGACTTTC TGCACAAGGA CCGGGATGAC GTCAATGTGG AGATCGTTTT TCTTCACAAC ATCTCCCCCG ACCTGGAGCT TGAAGCTCTG TTCAAACGAC ATTTTACTCA GGTGGAATTT TATCAGGGTT CCGTCCTCAA TCCACATGAT CTTGCAAGAG TCAAGATAGA GTCAGCAGAT GCATGCCTGA TCCTTGCCAA CAAGTACTGC GCTGACCCGG ATGCGGAGGA TGCCTCGAAT ATCATGAGAG TAATCTCCAT AAAGAACTAC CATCCGAAGA TAAGAATCAT CACTCAAATG CTGCAGTATC ACAACAAGGC CCATCTGCTA AACATCCCGA GCTGGAATTG GAAAGAAGGT GATGACGCAA TCTGCCTCGC AGAGTTGAAG TTGGGCTTCA TAGCCCAGAG CTGCCTGGCT CAAGGCCTCT CCACCATGCT TGCCAACCTC TTCTCCATGA GGTCATTCAT AAAGATTGAG GAAGACACAT GGCAGAAATA CTACTTGGAA GGAGTCTCAA ATGAAATGTA CACAGAATAT CTCTCCAGTG CCTTCGTGGG TCTGTCCTTC CCTACTGTTT GTGAGCTGTG TTTTGTGAAG CTCAAGCTCC TAATGATAGC CATTGAGTAC AAGTCTGCCA ACCGAGAGAG CCGTATATTA ATTAATCCTG GAAACCATCT TAAGATCCAA GAAGGTACTT TAGGATTTTT CATCGCAAGT GATGCCAAAG AAGTTAAAAG GGCATTTTTT TACTGCAAGG CCTGTCATGA TGACATCACA GATCCCAAAA GAATAAAAAA ATGTGGCTGC AAACGGCTTG AAGATGAGCA GCCGTCAACA CTATCACCAA AAAAAAAGCA ACGGAATGGA GGCATGCGGA ACTCACCCAA CACCTCGCCT AAGCTGATGA GGCATGACCC CTTGTTAATT CCTGGCAATG ATCAGATTGA CAACATGGAC TCCAATGTGA AGAAGTACGA CTCTACTGGG ATGTTTCACT GGTGTGCACC CAAGGAGATA GAGAAAGTCA TCCTGACTCG AAGTGAAGCT GCCATGACCG TCCTGAGTGG CCATGTCGTG GTCTGCATCT TTGGCGACGT CAGCTCAGCC CTGATCGGCC TCCGGAACCT GGTGATGCCG CTCCGTGCCA GCAACTTTCA TTACCATGAG CTCAAGCACA TTGTGTTTGT GGGCTCTATT GAGTACCTCA AGCGGGAATG GGAGACGCTT CATAACTTCC CCAAAGTGTC CATATTGCCT GGTACGCCAT TAAGTCGGGC TGATTTAAGG GCTGTCAACA TCAACCTCTG TGACATGTGC GTTATCCTGT CAGCCAATCA GAATAATATT GATGATACTT CGCTGCAGGA CAAGGAATGC ATCTTGGCGT CACTCAACAT CAAATCTATG CAGTTTGATG ACAGCATCGG AGTCTTGCAG GCTAATTCCC AAGGGTTCAC ACCTCCAGGA ATGGATAGAT CCTCTCCAGA TAACAGCCCA GTGCACGGGA TGTTACGTCA ACCATCCATC ACAACTGGGG TCAACATCCC CATCATCACT GAACTAGTGA ACGATACTAA TGTTCAGTTT TTGGACCAAG ACGATGATGA TGACCCTGAT ACAGAACTGT ACCTCACGCA GCCCTTTGCC TGTGGGACAG CATTTGCCGT CAGTGTCCTG GACTCACTCA TGAGCGCGAC GTACTTCAAT GACAATATCC TCACCCTGAT ACGGACCCTG GTGACCGGAG GAGCCACGCC GGAGCTGGAG GCTCTGATTG CTGAGGAAAA CGCCCTTAGA GGTGGCTACA GCACCCCGCA GACACTGGCC AATAGGGACC GCTGCCGCGT GGCCCAGTTA GCTCTGCTCG ATGGGCCATT TGCGGACTTA GGGGATGGTG GTTGTTATGG TGATCTGTTC TGCAAAGCTC TGAAAACATA TAATATGCTT TGTTTTGGAA TTTACCGGCT GAGAGATGCT CACCTCAGCA CCCCCAGTCA GTGCGCAAAG AGGTATGTCA TCACCAACCC GCCCTATGAG TTTGAGCTCG TGCCGACGGA CCTGATCTTC TGCTTAATGC AGTTTGACCA CAATGCCGGC CAGTCCCGGG CCAGCCTGTC CCATTCCTCC CACTCGTCGC AGTCCTCCAG CAAGAAGAGC TCCTCTGTTC ACTCCATCCC ATCCACAGCA AACCGACAGA ACCGGCCCAA GTCCAGGGAG TCCCGGGACA AACAGAAGTA CGTGCAGGAA GAGCGGCTTG GTACCGGCGA CTACAAGGAC GACGATGACA AGTAAGCGGC CGCGGGGATC CAGACATGAT AAGATACATT GATGAGTTTG GACAAACCAC AACTAGAATG CAGTGAAAAA AATGCTTTAT TTGTGAAATT TGTGATGCTA TTGCTTTATT TGTAACCATT ATAAGCTGCA ATAAACAAGT TAACAACAAC AATTGCATTC ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTCGGATCCT CTAGAGTCGA TCTGCAGGCA TGCTAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG AGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT ATGCGGCATC AGAGCAGATT GTACTGAGAG TGCACCATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGC GCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC TATTACGCCA GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG GTAACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGT
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