pGWB18
$128.00
In stock
SKU
PL84164
100μg liquid plasmid(Instantly):$149
| name | pGWB18 | another name | apGWB18 |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Protein expression | Fragment type | ORF,GateWay |
| Fragment species | Empty vector | Prokaryotic resistance | Kan |
| Selective marker | Neo/G418,Hyg | Fluorescent labeling | |
| Promoter | 35s | Replicon | oriV |
| Competence | DB3.1 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGAGCGTCGC AAAGGCGCTC GGTCTTGCCT TGCTCGTCGG TGATGTACTT CACCAGCTCC GCGAAGTCGC TCTTCTTGAT GGAGCGCATG GGGACGTGCT TGGCAATCAC GCGCACCCCC CGGCCGTTTT AGCGGCTAAA AAAGTCATGG CTCTGCCCTC GGGCGGACCA CGCCCATCAT GACCTTGCCA AGCTCGTCCT GCTTCTCTTC GATCTTCGCC AGCAGGGCGA GGATCGTGGC ATCACCGAAC CGCGCCGTGC GCGGGTCGTC GGTGAGCCAG AGTTTCAGCA GGCCGCCCAG GCGGCCCAGG TCGCCATTGA TGCGGGCCAG CTCGCGGACG TGCTCATAGT CCACGACGCC CGTGATTTTG TAGCCCTGGC CGACGGCCAG CAGGTAGGCC GACAGGCTCA TGCCGGCCGC CGCCGCCTTT TCCTCAATCG CTCTTCGTTC GTCTGGAAGG CAGTACACCT TGATAGGTGG GCTGCCCTTC CTGGTTGGCT TGGTTTCATC AGCCATCCGC TTGCCCTCAT CTGTTACGCC GGCGGTAGCC GGCCAGCCTC GCAGAGCAGG ATTCCCGTTG AGCACCGCCA GGTGCGAATA AGGGACAGTG AAGAAGGAAC ACCCGCTCGC GGGTGGGCCT ACTTCACCTA TCCTGCCCGG CTGACGCCGT TGGATACACC AAGGAAAGTC TACACGAACC CTTTGGCAAA ATCCTGTATA TCGTGCGAAA AAGGATGGAT ATACCGAAAA AATCGCTATA ATGACCCCGA AGCAGGGTTA TGCAGCGGAA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCAGA AGGCCGCCAG AGAGGCCGAG CGCGGCCGTG AGGCTTGGAC GCTAGGGCAG GGCATGAAAA AGCCCGTAGC GGGCTGCTAC GGGCGTCTGA CGCGGTGGAA AGGGGGAGGG GATGTTGTCT ACATGGCTCT GCTGTAGTGA GTGGGTTGCG CTCCGGCAGC GGTCCTGATC AATCGTCACC CTTTCTCGGT CCTTCAACGT TCCTGACAAC GAGCCTCCTT TTCGCCAATC CATCGACAAT CACCGCGAGT CCCTGCTCGA ACGCTGCGTC CGGACCGGCT TCGTCGAAGG CGTCTATCGC GGCCCGCAAC AGCGGCGAGA GCGGAGCCTG TTCAACGGTG CCGCCGCGCT CGCCGGCATC GCTGTCGCCG GCCTGCTCCT CAAGCACGGC CCCAACAGTG AAGTAGCTGA TTGTCATCAG CGCATTGACG GCGTCCCCGG CCGAAAAACC CGCCTCGCAG AGGAAGCGAA GCTGCGCGTC GGCCGTTTCC ATCTGCGGTG CGCCCGGTCG CGTGCCGGCA TGGATGCGCG CGCCATCGCG GTAGGCGAGC AGCGCCTGCC TGAAGCTGCG GGCATTCCCG ATCAGAAATG AGCGCCAGTC GTCGTCGGCT CTCGGCACCG AATGCGTATG ATTCTCCGCC AGCATGGCTT CGGCCAGTGC GTCGAGCAGC GCCCGCTTGT TCCTGAAGTG CCAGTAAAGC GCCGGCTGCT GAACCCCCAA CCGTTCCGCC AGTTTGCGTG TCGTCAGACC GTCTACGCCG ACCTCGTTCA ACAGGTCCAG GGCGGCACGG ATCACTGTAT TCGGCTGCAA CTTTGTCATG CTTGACACTT TATCACTGAT AAACATAATA TGTCCACCAA CTTATCAGTG ATAAAGAATC CGCGCGTTCA ATCGGACCAG CGGAGGCTGG TCCGGAGGCC AGACGTGAAA CCCAACATAC CCCTGATCGT AATTCTGAGC ACTGTCGCGC TCGACGCTGT CGGCATCGGC CTGATTATGC CGGTGCTGCC GGGCCTCCTG CGCGATCTGG TTCACTCGAA CGACGTCACC GCCCACTATG GCATTCTGCT GGCGCTGTAT GCGTTGGTGC AATTTGCCTG CGCACCTGTG CTGGGCGCGC TGTCGGATCG TTTCGGGCGG CGGCCAATCT TGCTCGTCTC GCTGGCCGGC GCCAGATCTG GGGAACCCTG TGGTTGGCAT GCACATACAA ATGGACGAAC GGATAAACCT TTTCACGCCC TTTTAAATAT CCGATTATTC TAATAAACGC TCTTTTCTCT TAGGTTTACC CGCCAATATA TCCTGTCAAA CACTGATAGT TTAAACTGAA GGCGGGAAAC GACAATCTGA TCATGAGCGG AGAATTAAGG GAGTCACGTT ATGACCCCCG CCGATGACGC GGGACAAGCC GTTTTACGTT TGGAACTGAC AGAACCGCAA CGTTGAAGGA GCCACTCAGC CGCGGGTTTC TGGAGTTTAA TGAGCTAAGC ACATACGTCA GAAACCATTA TTGCGCGTTC AAAAGTCGCC TAAGGTCACT ATCAGCTAGC AAATATTTCT TGTCAAAAAT GCTCCACTGA CGTTCCATAA ATTCCCCTCG GTATCCAATT AGAGTCTCAT ATTCACTCTC AATCCAAATA ATCTGCACCG GATCTGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GATGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACGGGATCTC TGCGGAACAG GCGGTCGAAG GTGCCGATAT CATTACGACA GCAACGGCCG ACAAGCACAA CGCCACGATC CTGAGCGACA ATATGATCGG GCCCGGCGTC CACATCAACG GCGTCGGCGG CGACTGCCCA GGCAAGACCG AGATGCACCG CGATATCTTG CTGCGTTCGG ATATTTTCGT GGAGTTCCCG CCACAGACCC GGATGATCCC CGATCGTTCA AACATTTGGC AATAAAGTTT CTTAAGATTG AATCCTGTTG CCGGTCTTGC GATGATTATC ATATAATTTC TGTTGAATTA CGTTAAGCAT GTAATAATTA ACATGTAATG CATGACGTTA TTTATGAGAT GGGTTTTTAT GATTAGAGTC CCGCAATTAT ACATTTAATA CGCGATAGAA AACAAAATAT AGCGCGCAAA CTAGGATAAA TTATCGCGCG CGGTGTCATC TATGTTACTA GATCGGGCCT CCTGTCAATG CTGGCGGCGG CTCTGGTGGT GGTTCTGGTG GCGGCTCTGA GGGTGGTGGC TCTGAGGGTG GCGGTTCTGA GGGTGGCGGC TCTGAGGGAG GCGGTTCCGG TGGTGGCTCT GGTTCCGGTG ATTTTGATTA TGAAAAGATG GCAAACGCTA ATAAGGGGGC TATGACCGAA AATGCCGATG AAAACGCGCT ACAGTCTGAC GCTAAAGGCA AACTTGATTC TGTCGCTACT GATTACGGTG CTGCTATCGA TGGTTTCATT GGTGACGTTT CCGGCCTTGC TAATGGTAAT GGTGCTACTG GTGATTTTGC TGGCTCTAAT TCCCAAATGG CTCAAGTCGG TGACGGTGAT AATTCACCTT TAATGAATAA TTTCCGTCAA TATTTACCTT CCCTCCCTCA ATCGGTTGAA TGTCGCCCTT TTGTCTTTGG CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGCCA AGCTTGCATG CCTGCAGGTC CCCAGATTAG CCTTTTCAAT TTCAGAAAGA ATGCTAACCC ACAGATGGTT AGAGAGGCTT ACGCAGCAGG TCTCATCAAG ACGATCTACC CGAGCAATAA TCTCCAGGAA ATCAAATACC TTCCCAAGAA GGTTAAAGAT GCAGTCAAAA GATTCAGGAC TAACTGCATC AAGAACACAG AGAAAGATAT ATTTCTCAAG ATCAGAAGTA CTATTCCAGT ATGGACGATT CAAGGCTTGC TTCACAAACC AAGGCAAGTA ATAGAGATTG GAGTCTCTAA AAAGGTAGTT CCCACTGAAT CAAAGGCCAT GGAGTCAAAG ATTCAAATAG AGGACCTAAC AGAACTCGCC GTAAAGACTG GCGAACAGTT CATACAGAGT CTCTTACGAC TCAATGACAA GAAGAAAATC TTCGTCAACA TGGTGGAGCA CGACACACTT GTCTACTCCA AAAATATCAA AGATACAGTC TCAGAAGACC AAAGGGCAAT TGAGACTTTT CAACAAAGGG TAATATCCGG AAACCTCCTC GGATTCCATT GCCCAGCTAT CTGTCACTTT ATTGTGAAGA TAGTGGAAAA GGAAGGTGGC TCCTACAAAT GCCATCATTG CGATAAAGGA AAGGCCATCG TTGAAGATGC CTCTGCCGAC AGTGGTCCCA AAGATGGACC CCCACCCACG AGGAGCATCG TGGAAAAAGA AGACGTTCCA ACCACGTCTT CAAAGCAAGT GGATTGATGT GATATCTCCA CTGACGTAAG GGATGACGCA CAATCCCACT ATCCTTCGCA AGACCCTTCC TCTATATAAG GAAGTTCATT TCATTTGGAG AGAACACGGG GGACTCTAGA ATGAGCGGGT TAATTAACGG TGAACAAAAG CTAATCTCCG AGGAAGACTT GAACGGTGAA CAAAAATTAA TCTCAGAAGA AGACTTGAAC GGACTCGACG GTGAACAAAA GTTGATTTCT GAAGAAGATT TGAACGGTGA ACAAAAGCTA ATCTCCGAGG AAGACTTGAA CGGTAGCGCT GTTATCACAA GTTTGTACAA AAAAGCTGAA CGAGAAACGT AAAATGATAT AAATATCAAT ATATTAAATT AGATTTTGCA TAAAAAACAG ACTACATAAT ACTGTAAAAC ACAACATATC CAGTCACTAT GGCGGCCCCT CGAGGGGCCG CATTAGGCAC CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATAATGTG TGGATTTTGA GTTAGGATCC GGCGAGATTT TCAGGAGCTA AGGAAGCTAA AATGGAGAAA AAAATCACTG GATATACCAC CGTTGATATA TCCCAATGGC ATCGTAAAGA ACATTTTGAG GCATTTCAGT CAGTTGCTCA ATGTACCTAT AACCAGACCG TTCAGCTGGA TATTACGGCC TTTTTAAAGA CCGTAAAGAA AAATAAGCAC AAGTTTTATC CGGCCTTTAT TCACATTCTT GCCCGCCTGA TGAATGCTCA TCCGGAATTC CGTATGGCAA TGAAAGACGG TGAGCTGGTG ATATGGGATA GTGTTCACCC TTGTTACACC GTTTTCCATG AGCAAACTGA AACGTTTTCA TCGCTCTGGA GTGAATACCA CGACGATTTC CGGCAGTTTC TACACATATA TTCGCAAGAT GTGGCGTGTT ACGGTGAAAA CCTGGCCTAT TTCCCTAAAG GGTTTATTGA GAATATGTTT TTCGTCTCAG CCAATCCCTG GGTGAGTTTC ACCAGTTTTG ATTTAAACGT GGCCAATATG GACAACTTCT TCGCCCCCGT TTTCACCATG GGCAAATATT ATACGCAAGG CGACAAGGTG CTGATGCCGC TGGCGATTCA GGTTCATCAT GCCGTCTGTG ATGGCTTCCA TGTCGGCAGA ATGCTTAATG AATTACAACA GTACTGCGAT GAGTGGCAGG GCGGGGCGTA AACGCGTGGA TCCGGCTTAC TAAAAGCCAG ATAACAGTAT GCGTATTTGC GCGCTGATTT TTGCGGTATA AGAATATATA CTGATATGTA TACCCGAAGT ATGTCAAAAA GAGGTGTGCT ATGAAGCAGC GTATTACAGT GACAGTTGAC AGCGACAGCT ATCAGTTGCT CAAGGCATAT ATGATGTCAA TATCTCCGGT CTGGTAAGCA CAACCATGCA GAATGAAGCC CGTCGTCTGC GTGCCGAACG CTGGAAAGCG GAAAATCAGG AAGGGATGGC TGAGGTCGCC CGGTTTATTG AAATGAACGG CTCTTTTGCT GACGAGAACA GGGACTGGTG AAATGCAGTT TAAGGTTTAC ACCTATAAAA GAGAGAGCCG TTATCGTCTG TTTGTGGATG TACAGAGTGA TATTATTGAC ACGCCCGGGC GACGGATGGT GATCCCCCTG GCCAGTGCAC GTCTGCTGTC AGATAAAGTC TCCCGTGAAC TTTACCCGGT GGTGCATATC GGGGATGAAA GCTGGCGCAT GATGACCACC GATATGGCCA GTGTGCCGGT CTCCGTTATC GGGGAAGAAG TGGCTGATCT CAGCCACCGC GAAAATGACA TCAAAAACGC CATTAACCTG ATGTTCTGGG GAATATAAAT GTCAGGCTCC CTTATACACA GCCAGTCTGC AGGTCGACCA TAGTGACTGG ATATGTTGTG TTTTACAGTA TTATGTAGTC TGTTTTTTAT GCAAAATCTA ATTTAATATA TTGATATTTA TATCATTTTA CGTTTCTCGT TCAGCTTTCT TGTACAAAGT GGTGATAACC TAGAGCTCGA ATTTCCCCGA TCGTTCAAAC ATTTGGCAAT AAAGTTTCTT AAGATTGAAT CCTGTTGCCG GTCTTGCGAT GATTATCATA TAATTTCTGT TGAATTACGT TAAGCATGTA ATAATTAACA TGTAATGCAT GACGTTATTT ATGAGATGGG TTTTTATGAT TAGAGTCCCG CAATTATACA TTTAATACGC GATAGAAAAC AAAATATAGC GCGCAAACTA GGATAAATTA TCGCGCGCGG TGTCATCTAT GTTACTAGAT CGGGAATTAG CTTCATCAAC GCAAGACATG CGCACGACCG TCTGACAGGA GAGGAATTTC CGACGAGCAC AGAAAGGACT TGCTCTTGGA CGTAGGCCTA TTTCTCAGGC ACATGTATCA AGTGTTCGGA CGTGGGTTTT CGATGGTGTA TCAGCCGCCG CCAACTGGGA GATGAGGAGG CTTTCTTGGG GGGCAGTCAG CAGTTCATTT CACAAGACAG AGGAACTTGT AAGGAGATGC ACTGATTTAT CTTGGCGCAA ACCAGCAGGA CGAATTAGTG GGAATAGCCC GCGAATATCT AAGTTATGCC TGTCGGCATG AGCAGAAACT TCCAATTCGA AACAGTTTGG AGAGGTTGTT TTTGGGCATA CCTTTTGTTA GTCAGCCTCT CGATTGCTCA TCGTCATTAC ACAGTACCGA AGTTTGATCG ATCTAGTAAC ATAGATGACA CCGCGCGCGA TAATTTATCC TAGTTTGCGC GCTATATTTT GTTTTCTATC GCGTATTAAA TGTATAATTG CGGGACTCTA ATCATAAAAA CCCATCTCAT AAATAACGTC ATGCATTACA TGTTAATTAT TACATGCTTA ACGTAATTCA ACAGAAATTA TATGATAATC ATCGCAAGAC CGGCAACAGG ATTCAATCTT AAGAAACTTT ATTGCCAAAT GTTTGAACGA TCTGCTTCGA CGCACTCCTT CTTTACTCCA CCATCTCGTC CTTATTGAAA ACGTGGGTAG CACCAAAACG AATCAAGTCG CTGGAACTGA AGTTACCAAT CACGCTGGAT GATTTGCCAG TTGGATTAAT CTTGCCTTTC CCCGCATGAA TAATATTGAT GAATGCATGC GTGAGGGGTA TTTCGATTTT GGCAATAGCT GCAATTGCCG CGACATCCTC CAACGAGCAT AATTCTTCAG AAAAATAGCG ATGTTCCATG TTGTCAGGGC ATGCATGATG CACGTTATGA GGTGACGGTG CTAGGCAGTA TTCCCTCAAA GTTTCATAGT CAGTATCATA TTCATCATTG CATTCCTGCA AGAGAGAATT GAGACGCAAT CCACACGCTG CGGCAACCTT CCGGCGTTCG TGGTCTATTT GCTCTTGGAC GTTGCAAACG TAAGTGTTGG ATCCCGGTCG GCATCTACTC TATTCCTTTG CCCTCGGACG AGTGCTGGGG CGTCGGTTTC CACTATCGGC GAGTACTTCT ACACAGCCAT CGGTCCAGAC GGCCGCGCTT CTGCGGGCGA TTTGTGTACG CCCGACAGTC CCGGCTCCGG ATCGGACGAT TGCGTCGCAT CGACCCTGCG CCCAAGCTGC ATCATCGAAA TTGCCGTCAA CCAAGCTCTG ATAGAGTTGG TCAAGACCAA TGCGGAGCAT ATACGCCCGG AGCCGCGGCG ATCCTGCAAG CTCCGGATGC CTCCGCTCGA AGTAGCGCGT CTGCTGCTCC ATACAAGCCA ACCACGGCCT CCAGAAGAAG ATGTTGGCGA CCTCGTATTG GGAATCCCCG AACATCGCCT CGCTCCAGTC AATGACCGCT GTTATGCGGC CATTGTCCGT CAGGACATTG TTGGAGCCGA AATCCGCGTG CACGAGGTGC CGGACTTCGG GGCAGTCCTC GGCCCAAAGC ATCAGCTCAT CGAGAGCCTG CGCGACGGAC GCACTGACGG TGTCGTCCAT CACAGTTTGC CAGTGATACA CATGGGGATC AGCAATCGCG CATATGAAAT CACGCCATGT AGTGTATTGA CCGATTCCTT GCGGTCCGAA TGGGCCGAAC CCGCTCGTCT GGCTAAGATC GGCCGCAGCG ATCGCATCCA TGGCCTCCGC GACCGGCTGC AGAACAGCGG GCAGTTCGGT TTCAGGCAGG TCTTGCAACG TGACACCCTG TGCACGGCGG GAGATGCAAT AGGTCAGGCT CTCGCTGAAT TCCCCAATGT CAAGCACTTC CGGAATCGGG AGCGCGGCCG ATGCAAAGTG CCGATAAACA TAACGATCTT TGTAGAAACC ATCGGCGCAG CTATTTACCC GCAGGACATA TCCACGCCCT CCTACATCGA AGCTGAAAGC ACGAGATTCT TCGCCCTCCG AGAGCTGCAT CAGGTCGGAG ACGCTGTCGA ACTTTTCGAT CAGAAACTTC TCGACAGACG TCGCGGTGAG TTCAGGCTTT TTCATATCGG GGTCGTCCTC TCCAAATGAA ATGAACTTCC TTATATAGAG GAAGGGTCTT GCGAAGGATA GTGGGATTGT GCGTCATCCC TTACGTCAGT GGAGATATCA CATCAATCCA CTTGCTTTGA AGACGTGGTT GGAACGTCTT CTTTTTCCAC GATGCTCCTC GTGGGTGGGG GTCCATCTTT GGGACCACTG TCGGCAGAGG CATCTTGAAC GATAGCCTTT CCTTTATCGC AATGATGGCA TTTGTAGGTG CCACCTTCCT TTTCTACTGT CCTTTTGATG AAGTGACAGA TAGCTGGGCA ATGGAATCCG AGGAGGTTTC CCGATATTAC CCTTTGTTGA AAAGTCTCAA TAGCCCTTTG GTCTTCTGAG ACTGTATCTT TGATATTCTT GGAGTAGACG AGAGTGTCGT GCTCCACCAT GTTGACGGAT CTCTAGGACG CGTCCTAGAA GCTAATTCAC TGGCCGTCGT TTTACAACGT CGTGACTGGG AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA TCCCCCTTTC GCCAGCTGGC GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA GTTGCGCAGC CTGAATGGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTTGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGG CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGAA CCACCATCAA ACAGGATTTT CGCCTGCTGG GGCAAACCAG CGTGGACCGC TTGCTGCAAC TCTCTCAGGG CCAGGCGGTG AAGGGCAATC AGCTGTTGCC CGTCTCACTG GTGAAAAGAA AAACCACCCC AGTACATTAA AAACGTCCGC AATGTGTTAT TAAGTTGTCT AAGCGTCAAT TTGTTTACAC CACAATATAT CCTGCCACCA GCCAGCCAAC AGCTCCCCGA CCGGCAGCTC GGCACAAAAT CACCACTCGA TACAGGCAGC CCATCAGTCC GGGACGGCGT CAGCGGGAGA GCCGTTGTAA GGCGGCAGAC TTTGCTCATG TTACCGATGC TATTCGGAAG AACGGCAACT AAGCTGCCGG GTTTGAAACA CGGATGATCT CGCGGAGGGT AGCATGTTGA TTGTAACGAT GACAGAGCGT TGCTGCCTGT GATCAAATAT CATCTCCCTC GCAGAGATCC GAATTATCAG CCTTCTTATT CATTTCTCGC TTAACCGTGA CAGGCTGTCG ATCTTGAGAA CTATGCCGAC ATAATAGGAA ATCGCTGGAT AAAGCCGCTG AGGAAGCTGA GTGGCGCTAT TTCTTTAGAA GTGAACGTTG ACGATATCAA CTCCCCTATC CATTGCTCAC CGAATGGTAC AGGTCGGGGA CCCGAAGTTC CGACTGTCGG CCTGATGCAT CCCCGGCTGA TCGACCCCAG ATCTGGGGCT GAGAAAGCCC AGTAAGGAAA CAACTGTAGG TTCGAGTCGC GAGATCCCCC GGAACCAAAG GAAGTAGGTT AAACCCGCTC CGATCAGGCC GAGCCACGCC AGGCCGAGAA CATTGGTTCC TGTAGGCATC GGGATTGGCG GATCAAACAC TAAAGCTACT GGAACGAGCA GAAGTCCTCC GGCCGCCAGT TGCCAGGCGG TAAAGGTGAG CAGAGGCACG GGAGGTTGCC ACTTGCGGGT CAGCACGGTT CCGAACGCCA TGGAAACCGC CCCCGCCAGG CCCGCTGCGA CGCCGACAGG ATCTAGCGCT GCGTTTGGTG TCAACACCAA CAGCGCCACG CCCGCAGTTC CGCAAATAGC CCCCAGGACC GCCATCAATC GTATCGGGCT ACCTAGCAGA GCGGCAGAGA TGAACACGAC CATCAGCGGC TGCACAGCGC CTACCGTCGC CGCGACCCCG CCCGGCAGGC GGTAGACCGA AATAAACAAC AAGCTCCAGA ATAGCGAAAT ATTAAGTGCG CCGAGGATGA AGATGCGCAT CCACCAGATT CCCGTTGGAA TCTGTCGGAC GATCATCACG AGCAATAAAC CCGCCGGCAA CGCCCGCAGC AGCATACCGG CGACCCCTCG GCCTCGCTGT TCGGGCTCCA CGAAAACGCC GGACAGATGC GCCTTGTGAG CGTCCTTGGG GCCGTCCTCC TGTTTGAAGA CCGACAGCCC AATGATCTCG CCGTCGATGT AGGCGCCGAA TGCCACGGCA TCTCGCAACC GTTCAGCGAA CGCCTCCATG GGCTTTTTCT CCTCGTGCTC GTAAACGGAC CCGAACATCT CTGGAGCTTT CTTCAGGGCC GACAATCGGA TCTCGCGGAA ATCCTGCACG TCGGCCGCTC CAAGCCGTCG AATCTGAGCC TTAATCACAA TTGTCAATTT TAATCCTCTG TTTATCGGCA GTTCGTAGAG CGCGCCGTGC GTCCCGAGCG ATACTGAGCG AAGCAAGTGC GTCGAGCAGT GCCCGCTTGT TCCTGAAATG CCAGTAAAGC GCTGGCTGCT GAACCCCCAG CCGGAACTGA CCCCACAAGG CCCTAGCGTT TGCAATGCAC CAGGTCATCA TTGACCCAGG CGTGTTCCAC CAGGCCGCTG CCTCGCAACT CTTCGCAGGC TTCGCCGACC TGCTCGCGCC ACTTCTTCAC GCGGGTGGAA TCCGATCCGC ACATGAGGCG GAAGGTTTCC AGCTTGAGCG GGTACGGCTC CCGGTGCGAG CTGAAATAGT CGAACATCCG TCGGGCCGTC GGCGACAGCT TGCGGTACTT CTCCCATATG AATTTCGTGT AGTGGTCGCC AGCAAACAGC ACGACGATTT CCTCGTCGAT CAGGACCTGG CAACGGGACG TTTTCTTGCC ACGGTCCAGG ACGCGGAAGC GGTGCAGCAG CGACACCGAT TCCAGGTGCC CAACGCGGTC GGACGTGAAG CCCATCGCCG TCGCCTGTAG GCGCGACAGG CATTCCTCGG CCTTCGTGTA ATACCGGCCA TTGATCGACC AGCCCAGGTC CTGGCAAAGC TCGTAGAACG TGAAGGTGAT CGGCTCGCCG ATAGGGGTGC GCTTCGCGTA CTCCAACACC TGCTGCCACA CCAGTTCGTC ATCGTCGGCC CGCAGCTCGA CGCCGGTGTA GGTGATCTTC ACGTCCTTGT TGACGTGGAA AATGACCTTG TTTTGCAGCG CCTCGCGCGG GATTTTCTTG TTGCGCGTGG TGAACAGGGC AGAGCGGGCC GTGTCGTTTG GCATCGCTCG CATCGTGTCC GGCCACGGCG CAATATCGAA CAAGGAAAGC TGCATTTCCT TGATCTGCTG CTTCGTGTGT TTCAGCAACG CGGCCTGCTT GGCCTCGCTG ACCTGTTTTG CCAGGTCCTC GCCGGCGGTT TTTCGCTTCT TGGTCGTCAT AGTTCCTCGC GTGTCGATGG TCATCGACTT CGCCAAACCT GCCGCCTCCT GTTCGAGACG ACGCGAACGC TCCACGGCGG CCGATGGCGC GGGCAGGGCA GGGGGAGCCA GTTGCACGCT GTCGCGCTCG ATCTTGGCCG TAGCTTGCTG GACCATCGAG CCGACGGACT GGAAGGTTTC GCGGGGCGCA CGCATGACGG TGCGGCTTGC GATGGTTTCG GCATCCTCGG CGGAAAACCC CGCGTCGATC AGTTCTTGCC TGTATGCCTT CCGGTCAAAC GTCCGATTCA TTCACCCTCC TTGCGGGATT GCCCCGACTC ACGCCGGGGC AATGTGCCCT TATTCCTGAT TTGACCCGCC TGGTGCCTTG GTGTCCAGAT AATCCACCTT ATCGGCAATG AAGTCGGTCC CGTAGACCGT CTGGCCGTCC TTCTCGTACT TGGTATTCCG AATCTTGCCC TGCACGAATA CCAGCGACCC CTTGCCCAAA TACTTGCCGT GGGCCTCGGC CTGAGAGCCA AAACACTTGA TGCGGAAGAA GTCGGTGCGC TCCTGCTTGT CGCCGGCATC GTTGCGCCAC ATCTAGGTAC TAAAACAATT CATCCAGTAA AATATAATAT TTTATTTTCT CCCAATCAGG CTTGATCCCC AGTAAGTCAA AAAATAGCTC GACATACTGT TCTTCCCCGA TATCCTCCCT GATCGACCGG ACGCAGAAGG CAATGTCATA CCACTTGTCC GCCCTGCCGC TTCTCCCAAG ATCAATAAAG CCACTTACTT TGCCATCTTT CACAAAGATG TTGCTGTCTC CCAGGTCGCC GTGGGAAAAG ACAAGTTCCT CTTCGGGCTT TTCCGTCTTT AAAAAATCAT ACAGCTCGCG CGGATCTTTA AATGGAGTGT CTTCTTCCCA GTTTTCGCAA TCCACATCGG CCAGATCGTT ATTCAGTAAG TAATCCAATT CGGCTAAGCG GCTGTCTAAG CTATTCGTAT AGGGACAATC CGATATGTCG ATGGAGTGAA AGAGCCTGAT GCACTCCGCA TACAGCTCGA TAATCTTTTC AGGGCTTTGT TCATCTTCAT ACTCTTCCGA GCAAAGGACG CCATCGGCCT CACTCATGAG CAGATTGCTC CAGCCATCAT GCCGTTCAAA GTGCAGGACC TTTGGAACAG GCAGCTTTCC TTCCAGCCAT AGCATCATGT CCTTTTCCCG TTCCACATCA TAGGTGGTCC CTTTATACCG GCTGTCCGTC ATTTTTAAAT ATAGGTTTTC ATTTTCTCCC ACCAGCTTAT ATACCTTAGC AGGAGACATT CCTTCCGTAT CTTTTACGCA GCGGTATTTT TCGATCAGTT TTTTCAATTC CGGTGATATT CTCATTTTAG CCATTTATTA TTTCCTTCCT CTTTTCTACA GTATTTAAAG ATACCCCAAG AAGCTAATTA TAACAAGACG AACTCCAATT CACTGTTCCT TGCATTCTAA AACCTTAAAT ACCAGAAAAC AGCTTTTTCA AAGTTGTTTT CAAAGTTGGC GTATAACATA GTATCGACGG AGCCGATTTT GAAACCACAA TTATGGGTGA TGCTGCCAAC TTACTGATTT AGTGTATGAT GGTGTTTTTG AGGTGCTCCA GTGGCTTCTG TGTCTATCAG CTGTCCCTCC TGTTCAGCTA CTGACGGGGT GGTGCGTAAC GGCAAAAGCA CCGCCGGACA TCAGCGCTAT CTCTGCTCTC ACTGCCGTAA AACATGGCAA CTGCAGTTCA CTTACACCGC TTCTCAACCC GGTACGCACC AGAAAATCAT TGATATGGCC ATGAATGGCG TTGGATGCCG GGCAACAGCC CGCATTATGG GCGTTGGCCT CAACACGATT TTACGTCACT TAAAAAACTC AGGCCGCAGT CGGTAACCTC GCGCATACAG CCGGGCAGTG ACGTCATCGT CTGCGCGGAA ATGGACGAAC AGTGGGGCTA TGTCGGGGCT AAATCGCGCC AGCGCTGGCT GTTTTACGCG TATGACAGTC TCCGGAAGAC GGTTGTTGCG CACGTATTCG GTGAACGCAC TATGGCGACG CTGGGGCGTC TTATGAGCCT GCTGTCACCC TTTGACGTGG TGATATGGAT GACGGATGGC TGGCCGCTGT ATGAATCCCG CCTGAAGGGA AAGCTGCACG TAATCAGCAA GCGATATACG CAGCGAATTG AGCGGCATAA CCTGAATCTG AGGCAGCACC TGGCACGGCT GGGACGGAAG TCGCTGTCGT TCTCAAAATC GGTGGAGCTG CATGACAAAG TCATCGGGCA TTATCTGAAC ATAAAACACT ATCAATAAGT TGGAGTCATT ACCCAATTAT GATAGAATTT ACAAGCTATA AGGTTATTGT CCTGGGTTTC AAGCATTAGT CCATGCAAGT TTTTATGCTT TGCCCATTCT ATAGATATAT TGATAAGCGC GCTGCCTATG CCTTGCCCCC TGAAATCCTT ACATACGGCG ATATCTTCTA TATAAAAGAT ATATTATCTT ATCAGTATTG TCAATATATT CAAGGCAATC TGCCTCCTCA TCCTCTTCAT CCTCTTCGTC TTGGTAGCTT TTTAAATATG GCGCTTCATA GAGTAATTCT GTAAAGGTCC AATTCTCGTT TTCATACCTC GGTATAATCT TACCTATCAC CTCAAATGGT TCGCTGGGTT TATCGCACCC CCGAACACGA GCACGGCACC CGCGACCACT ATGCCAAGAA TGCCCAAGGT AAAAATTGCC GGCCCCGCCA TGAAGTCCGT GAATGCCCCG ACGGCCGAAG TGAAGGGCAG GCCGCCACCC AGGCCGCCGC CCTCACTGCC CGGCACCTGG TCGCTGAATG TCGATGCCAG CACCTGCGGC ACGTCAATGC TTCCGGGCGT CGCGCTCGGG CTGATCGCCC ATCCCGTTAC TGCCCCGATC CCGGCAATGG CAAGGACTGC CAGCGCTGCC ATTTTTGGGG TGAGGCCGTT CGCGGCCGAG GGGCGCAGCC CCTGGGGGGA TGGGAGGCCC GCGTTAGCGG GCCGGGAGGG TTCGAGAAGG GGGGGCACCC CCCTTCGGCG TGCGCGGTCA CGCGCACAGG GCGCAGCCCT GGTTAAAAAC AAGGTTTATA AATATTGGTT TAAAAGCAGG TTAAAAGACA GGTTAGCGGT GGCCGAAAAA CGGGCGGAAA CCCTTGCAAA TGCTGGATTT TCTGCCTGTG GACAGCCCCT CAAATGTCAA TAGGTGCGCC CCTCATCTGT CAGCACTCTG CCCCTCAAGT GTCAAGGATC GCGCCCCTCA TCTGTCAGTA GTCGCGCCCC TCAAGTGTCA ATACCGCAGG GCACTTATCC CCAGGCTTGT CCACATCATC TGTGGGAAAC TCGCGTAAAA TCAGGCGTTT TCGCCGATTT GCGAGGCTGG CCAGCTCCAC GTCGCCGGCC GAAATCGAGC CTGCCCCTCA TCTGTCAACG CCGCGCCGGG TGAGTCGGCC CCTCAAGTGT CAACGTCCGC CCCTCATCTG TCAGTGAGGG CCAAGTTTTC CGCGAGGTAT CCACAACGCC GGCGGCCGCG GTGTCTCGCA CACGGCTTCG ACGGCGTTTC TGGCGCGTTT GCAGGGCCAT AGACGGCCGC CAGCCCAGCG GCGAGGGCAA CCAGCCCGG
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