pOst1-NubI
$99.00
In stock
SKU
PL84148
100μg liquid plasmid(Instantly):$120
| name | pOst1-NubI | another name | apOst1-NubI |
| Insert Size (bp) | Plasmid host | Yeast bacterium | |
| Plasmid uses | hybridization | Fragment type | |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | TRP1 | Fluorescent labeling | |
| Promoter | Replicon | ||
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG CAACGCAATT AATGTGAGTT AGCTCACTCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT ATGTTGTGTG GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCTA TGACCATGAT TACGCCAAGC TTGCATGCCG GTAGAGGTGT GGTCAATAAG AGCGACCTCA TGCTATACCT GAGAAAGCAA CCTGACCTAC AGGAAAGAGT TACTCAAGAA TAAGAATTTT CGTTTTAAAA CCTAAGAGTC ACTTTAAAAT TTGTATACAC TTATTTTTTT TATAACTTAT TTAATAATAA AAATCATAAA TCATAAGAAA TTCGCCCGGA ATTAGCTTGG CTGCAGGCGG CCGCTCATGG TCGACCCACC AGGGATTCCT TCCTTGTCTT GAATTTTCGA CTTAACGTTG TCGATGGTAT CGGAAGATTC AACTTCCAAT GTTATGGTTT TACCGGTCAA AGTCTTGACG AAAATCTGCA TGGCTGCAGG TCGACGGATC TGAGCGTAAT CTGGAACATC GTATGGGTAC TGGATCTGAG CGTAATCTGG AACATCGTAT GGGTACTGGA TCCCGAGCTC TGATCCGTTA GTAACGTTCA TGTTCAAAGT TTTTAAGACA AAAACTCCCA TTAGTGCGGT GAAAATATAG CAAGCAATAG ACAGAGGCTT CTTGAAAAAA GAGCTTTTCG GGTAGTCGTA CTTTATCAAG ACTTGGCCAT TGGCAACTTG ACTAATCAAA TTTCTGTAAC TGAAAGTTAA TTTAACGTGA CCTTTATTTA GGTCAAAGTA TGATTTCTGG GTTTCTATAG AAACATTTGT AAAAGGGACT GGAGAATCAA TATCGAATAT TTCGGCCCCT TCCGGAAGAA ATACCGATAA TTCAACATTA TCATATACAG TGTCCGGTGG GCCGTTTAGA ATTGGGATAG AAGCAACGAA TTTCTCGTCT GAGCCAGAGG ATACATGCAA GAAATCGGAC AATTTATTAG TCCAACCGAC AGTAAAATTG TAGTTCCAAC CTCCAAAGAT TGGGAATCTT GGTCTTATAA AGAAATGGTC ACGTTCTGCA TGCGACGTGG AAACAAGGCC AACCAAATCA GTGAAATAAT GATCAGTAGC TCCCTCAGGC AGGAGCATGT CTAAGACAGT AACAAAGTGA GTTTGTCTCA TATTCTGAGT TTGAATCTGT TTCATTAATT CTAATCTTGA AAATCCTTTA GACAGTTTTG CGGCTTTGTT TGTTAATTCA TAATATTCCT CAAATTGTAT TGTGGAAGCC CAATGAGAAA GCCAAATATC TCTTCTCAAA TTCACTACCT GATTCAATGG GGCATTGTGG GAATAAACAA TTGCTAAGGT TTCGTTTGAA GAAAATCTCG GAATATCTTC CCAAGGCCCA AACTCAAAAG AGTTTCCATT AGCTTTTCCC AGTAAACTCT CGTCATTTGG GGGGTGGTAC TCCTCAAATG ATGAGCTACC AATCAGCGTA AAAGAGGCCT TCTTGGTATC GTAAGCAGAA AGCGGCAATC TGTTCGTTTC CCACAATAGG TGTTGTTCTT CTGACATTCC AACGTGCTCA GGATAAGGAA TACCTACGGT ATTATAGAAG CTCTTAATCA CTAAAGAAAC TTCTTCCTGA GGGGAAATTG CATTTGGAAA TTGAATGATC CCGTATCTAA TTTCACTTTC ACCATCGTCA CCAGGTGCTG TAGTCGAATT GGCAAGTAAT TGGCTATTTA AAAAAGTTGC CTCGTTGGTA AAATAGGCTG AAAAAAAGGA AACTTTACTG AAGATGCCAC TCTCAAAGGC TGTGAAGTAT TCAGTCGCAG GTTCGCTTGC TATGTTTTTG ATAGTTATTT CGATTGTTTC TGAAATATAA GCGTTTGACA CGTCTATTGT CCTCTTATAA TCAACATTCT CCCAAGTCGC AGGTGGCTCG TATTGGGCAG CAGAAGACAC GTTGAAAAAA CATAGGAACA ATCCCACAAT CCAAGAGAAC CAAATCTGCC TCATATGAGT TGATTGTATG CTTGGTATAG CTTGAAATAT TGTGCAGAAA AAGAAACAAG GAAGAAAGGG AACGAGAACA ATGACGAGGA AACAAAAGAT TAATAATTGC AGGTCTATTT ATACTTGATA GCAAGACAGC AAACTTTTTT TTATTTCAAA TTCAAGTAAC TGGAAGGAAG GCCGTATACC GTTGCTCATT AGAGAGTAGT GTGCGTGAAT GAAGGAAGGA AAAAGTTTCG TGTGCTTCGA GATACCCCTC ATCAGCTCTG GAACAACGAC ATCTGTTGGT GCTGTCTTTG TCGTTAATTT TTTCCTTTAG TGTCTTCCAT CATTTTTTTG TCATTGCGGA TATGGTGAGA CAACAACGGG GGAGAGAGAA AAGAAAAAAA AAGAAAAGAA GTTGCATGCG CCTATTATTA CTTCAATAGA TGGCAAATGG AAAAAGGGTA GTGAAACTTC GATATGATGA TGGCTATCAA GTCTAGGGCT ACAGTATTAG TTCGTTATGT ACCACCATCA ATGAGGCAGT GTAATTGGTG TAGTCTTGTT TAGCCCATTA TGTCTTGTCT GGTATCTGTT CTATTGTATA TCTCCCCTCC GCCACCTACA TGTTAGGGAG ACCAACGAAG GTATTATAGG AATCCCGATG TATGGGTTTG GTTGCCAGAA AAGAGGAAGT CCATATTGTA CACCCGGAAA CAACAAAAGG ATATCGAGCT CGCGAAAGCT TGGCACTGGC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG GCCCGCACCG ATCGCCCTTC CCAACAGTTG CGCAGCCTGA ATGGCGAATG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGCTT ACAATTTCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAG ATCGGCAAGT GCACAAACAA TACTTAAATA AATACTACTC AGTAATAACC TATTTCTTAG CATTTTTGAC GAAATTTGCT ATTTTGTTAG AGTCTTTTAC ACCATTTGTC TCCACACCTC CGCTTACATC AACACCAATA ACGCCATTTA ATCTAAGCGC ATCACCAACA TTTTCTGGCG TCAGTCCACC AGCTAACATA AAATGTAAGC TTTCGGGGCT CTCTTGCCTT CCAACCCAGT CAGAAATCGA GTTCCAATCC AAAAGTTCAC CTGTCCCACC TGCTTCTGAA TCAAACAAGG GAATAAACGA ATGAGGTTTC TGTGAAGCTG CACTGAGTAG TATGTTGCAG TCTTTTGGAA ATACGAGTCT TTTAATAACT GGCAAACCGA GGAACTCTTG GTATTCTTGC CACGACTCAT CTCCATGCAG TTGGACGATA TCAATGCCGT AATCATTGAC CAGAGCCAAA ACATCCTCCT TAGGTTGATT ACGAAACACG CCAACCAAGT ATTTCGGAGT GCCTGAACTA TTTTTATATG CTTTTACAAG ACTTGAAATT TTCCTTGCAA TAACCGGGTC AATTGTTCTC TTTCTATTGG GCACACATAT AATACCCAGC AAGTCAGCAT CGGAATCTAG AGCACATTCT GCGGCCTCTG TGCTCTGCAA GCCGCAAACT TTCACCAATG GACCAGAACT ACCTGTGAAA TTAATAACAG ACATACTCCA AGCTGCCTTT GTGTGCTTAA TCACGTATAC TCACGTGCTC AATAGTCACC AATGCCCTCC CTCTTGGCCC TCTCCTTTTC TTTTTTCGAC CGAATTAATT CTTAATCGGC AAAAAAAGAA AAGCTCCGGA TCAAGATTGT ACGTAAGGTG ACAAGCTATT TTTCAATAAA GAATATCTTC CACTACTGCC ATCTGGCGTC ATAACTGCAA AGTACACATA TATTACGATG CTGTTCTATT AAATGCTTCC TATATTATAT ATATAGTAAT GTCGTTGATC TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GTATGATCCA ATATCAAAGG AAATGATAGC ATTGAAGGAT GAGACTAATC CAATTGAGGA GTGGCAGCAT ATAGAACAGC TAAAGGGTAG TGCTGAAGGA AGCATACGAT ACCCCGCATG GAATGGGATA ATATCACAGG AGGTACTAGA CTACCTTTCA TCCTACATAA ATAGACGCAT ATAAGTACGC ATTTAAGCAT AAACACGCAC TATGCCGTTC TTCTCATGTA TATATATATA CAGGCAACAC GCAGATATAG GTGCGACGTG AACAGTGAGC TGTATGTGCG CAGCTCGCGT TGCATTTTCG GAAGCGCTCG TTTTCGGAAA CGCTTTGAAG TTCCTATTCC GAAGTTCCTA TTCTCTAGAA AGTATAGGAA CTTCAGAGCG CTTTTGAAAA CCAAAAGCGC TCTGAAGACG CACTTTCAAA AAACCAAAAA CGCACCGGAC TGTAACGAGC TACTAAAATA TTGCGAATAC CGCTTCCACA AACATTGCTC AAAAGTATCT CTTTGCTATA TATCTCTGTG CTATATCCCT ATATAACCTA CCCATCCACC TTTCGCTCCT TGAACTTGCA TCTAAACTCG ACCTCTACAT TTTTTATGTT TATCTCTAGT ATTACTCTTT AGACAAAAAA ATTGTAGTAA GAACTATTCA TAGAGTGAAT CGAAAACAAT ACGAAAATGT AAACATTTCC TATACGTAGT ATATAGAGAC AAAATAGAAG AAACCGTTCA TAATTTTCTG ACCAATGAAG AATCATCAAC GCTATCACTT TCTGTTCACA AAGTATGCGC AATCCACATC GGTATAGAAT ATAATCGGGG ATGCCTTTAT CTTGAAAAAA TGCACCCGCA GCTTCGCTAG TAATCAGTAA ACGCGGGAAG TGGAGTCAGG CTTTTTTTAT GGAAGAGAAA ATAGACACCA AAGTAGCCTT CTTCTAACCT TAACGGACCT ACAGTGCAAA AAGTTATCAA GAGACTGCAT TATAGAGCGC ACAAAGGAGA AAAAAAGTAA TCTAAGATGC TTTGTTAGAA AAATAGCGCT CTCGGGATGC ATTTTTGTAG AACAAAAAAG AAGTATAGAT TCTTTGTTGG TAAAATAGCG CTCTCGCGTT GCATTTCTGT TCTGTAAAAA TGCAGCTCAG ATTCTTTGTT TGAAAAATTA GCGCTCTCGC GTTGCATTTT TGTTTTACAA AAATGAAGCA CAGATTCTTC GTTGGTAAAA TAGCGCTTTC GCGTTGCATT TCTGTTCTGT AAAAATGCAG CTCAGATTCT TTGTTTGAAA AATTAGCGCT CTCGCGTTGC ATTTTTGTTC TACAAAATGA AGCACAGATG CTTCGTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGG
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