pBlueBacHis2 A
$99.00
In stock
SKU
PL84130
100μg liquid plasmid(Instantly):$120
| name | pBlueBacHis2 A | another name | apBlueBacHis2 A |
| Insert Size (bp) | Plasmid host | Insect cells | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | ||
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | M13F：TGTAAAACGACGGCCAGT | |
| 3′ sequencing primer | M13R：CAGGAAACAGCTATGACC | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GATATCATGG AGATAATTAA AATGATAACC ATCTCGCAAA TAAATAAGTA TTTTACTGTT TTCGTAACAG TTTTGTAATA AAAAACCTAT AAATATGCCG CGGGGTTCTC ATCATCATCA TCATCATGGT ATGGCTAGCA TGACTGGTGG ACAGCAAATG GGTCGGGATC TGTACGACGA TGACGATAAG GATCGATGGG GATCCGAGCT CGAGATCTGC AGCTGGTACC ATGGAATTCG AAGCTTGGAG TCGACTCTGC TGAAGAGGAG GAAATTCTCC TTGAAGTTTC CCTGGTGTTC AAAGTAAAGG AGTTTGCACC AGACGCACCT CTGTTCACTG GTCCGGCGTA TTAAAACACG ATACATTGTT ATTAGTACAT TTATTAAGCG CTAGATTCTG TGCGTTGTTG ATTTACAGAC AATTGTTGTA CGTATTTTAA TAATTCATTA AATTTATAAT CTTTAGGGTG GTATGTTAGA GCGAAAATCA AATGATTTTC AGCGTCTTTA TATCTGAATT TAAATATTAA ATCCTCAATA GATTTGTAAA ATAGGTTTCG ATTAGTTTCA AACAAGGGTT GTTTTTCCGA ACCGATGGCT GGACTATCTA ATGGATTTTC GCTCAACGCC ACAAAACTTG CCAAATCTTG TAGCAGCAAT CTAGCTTTGT CGATATTCGT TTGTGTTTTG TTTTGTAATA AAGGTTCGAC GTCGTTCAAA ATATTATGCG CTTTTGTATT TCTTTCATCA CTGTCGTTAG TGTACAATTG ACTCGACGTA AACACGTTAA ATAAAGCCTG GACATATTTA ACATCGGGCG TGTTAGCTTT ATTAGGCCGA TTATCGTCGT CGTCCCAACC CTCGTCGTTA GAAGTTGCTT CCGAAGACGA TTTTGCCATA GCCACACGAC GCCTATTAAT TGTGTCGGCT AACACGTCCG CGATCAAATT TGTAGTTGAG CTTTTTGGAA TTATTTCTGA TTGCGGGCGT TTTTGGGCGG GTTTCAATCT AACTGTGCCC GATTTTAATT CAGACAACAC GTTAGAAAGC GATGGTGCAG GCGGTGGTAA CATTTCAGAC GGCAAATCTA CTAATGGCGG CGGTGGTGGA GCTGATGATA AATCTACCAT CGGTGGAGGC GCAGGCGGGG CTGGCGGCGG AGGCGGAGGC GGAGGTGGTG GCGGTGATGC AGACGGCGGT TTAGGCTCAA ATTGTCTCTT TCAGGCAACA CAGTCGGCAC CTCAACTATT GTACTGGTTT CGGGCGTATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCAT CCTGCACCAT CGTCTGCTCA TCCATGACCT GACCATGCAG AGGATGATGC TCGTGACGGT TAACGCCTCG AATCAGCAAC GGCTTGCCGT TCAGCAGCAG CAGACCATTT TCAATCCGCA CCTCGCGGAA ACCGACATCG CAGGCTTCTG CTTCAATCAG CGTGCCGTCG GCGGTGTGCA GTTCAACCAC CGCACGATAG AGATTCGGGA TTTCGGCGCT CCACAGTTTC GGGTTTTCGA CGTTCAGACG TAGTGTGACG CGATCGGTAT AACCACCACG CTCATCGATA ATTTCACCGC CGAAAGGCGC GGTGCCGCTG GCGACCTGCG TTTCACCCTG CCATAAAGAA ACTGTTACCC GTAGGTAGTC ACGCAACTCG CCGCACATCT GAACTTCAGC CTCCAGTACA GCGCGGCTGA AATCATCATT AAAGCGAGTG GCAACATGGA AATCGCTGAT TTGTGTAGTC GGTTTATGCA GCAACGAGAC GTCACGGAAA ATGCCGCTCA TCCGCCACAT ATCCTGATCT TCCAGATAAC TGCCGTCACT CCAACGCAGC ACCATCACCG CGAGGCGGTT TTCTCCGGCG CGTAAAAATG CGCTCAGGTC AAATTCAGAC GGCAAACGAC TGTCCTGGCC GTAACCGACC CAGCGCCCGT TGCACCACAG ATGAAACGCC GAGTTAACGC CATCAAAAAT AATTCGCGTC TGGCCTTCCT GTAGCCAGCT TTCATCAACA TTAAATGTGA GCGAGTAACA ACCCGTCGGA TTCTCCGTGG GAACAAACGG CGGATTGACC GTAATGGGAT AGGTCACGTT GGTGTAGATG GGCGCATCGT AACCGTGCAT CTGCCAGTTT GAGGGGACGA CGACAGTATC GGCCTCAGGA AGATCGCACT CCAGCCAGCT TTCCGGCACC GCTTCTGGTG CCGGAAACCA GGCAAAGCGC CATTCGCCAT TCAGGCTGCG CAACTGTTGG GAAGGGCGAT CGGTGCGGGC CTCTTCGCTA TTACGCCAGC TGGCGAAAGG GGGATGTGCT GCAAGGCGAT TAAGTTGGGT AACGCCAGGG TTTTCCCAGT CACGACGTTG TAAAACGACG GGATCTATCA TTTTTAGCAG TGATTCTAAT TGCAGCTGCT CTTTGATACA ACTAATTTTA CGACGACGAT GCGAGCTTTT ATTCAACCGA GCGTGCATGT TTGCAATCGT GCAAGCGTTA TCAATTTTTC ATTATCGTAT TGTTGCACAT CAACAGGCTG GACACCACGT TGAACTCGCC GCAGTTTTGC GGCAAGTTGG ACCCGCCGCG CATCCAATGC AAACTTTCCG ACATTCTGTT GCCTACGAAC GATTGATTCT TTGTCCATTG ATCGAAGCGA GTGCCTTCGA CTTTTTCGTG TCCAGTGTGG CTT
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