pCAG-D1R
$113.00
In stock
SKU
PL84114
100μg liquid plasmid(Instantly):$134
| name | pCAG-D1R | another name | apCAG-D1R |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Virus | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | |
| Promoter | CAG | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | pcaggs-F：GTTCGGCTTCTGGCGTGT | |
| 3′ sequencing primer | pcaggs-R：TATGTCCTTCCGAGTGAGAG | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GACATTGATT ATTGACTAGT TATTAATAGT AATCAATTAC GGGGTCATTA GTTCATAGCC CATATATGGA GTTCCGCGTT ACATAACTTA CGGTAAATGG CCCGCCTGGC TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA CGTATGTTCC CATAGTAACG CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC TGCCCACTTG GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA TGACGGTAAA TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG GTCGAGGTGA GCCCCACGTT CTGCTTCACT CTCCCCATCT CCCCCCCCTC CCCACCCCCA ATTTTGTATT TATTTATTTT TTAATTATTT TGTGCAGCGA TGGGGGCGGG GGGGGGGGGG GGGGGCGCCA GGCGGGGCGG GGCGGGGCGA GGGGCGGGGC GGGGCGAGGC GGAGAGGTGC GGCGGCAGCC AATCAGAGCG GCGCGCTCCG AAAGTTTCCT TTTATGGCGA GGCGGCGGCG GCGGCGGCCC TATAAAAAGC GAAGCGCGCG GCGGGCGGGA GTCGCTGCGT TGCCTTCGCC CCGTGCCCCG CTCCGCGCCG CCTCGCGCCG CCCGCCCCGG CTCTGACTGA CCGCGTTACT CCCACAGGTG AGCGGGCGGG ACGGCCCTTC TCCTCCGGGC TGTAATTAGC GCTTGGTTTA ATGACGGCTC GTTTCTTTTC TGTGGCTGCG TGAAAGCCTT AAAGGGCTCC GGGAGGGCCC TTTGTGCGGG GGGGAGCGGC TCGGGGGGTG CGTGCGTGTG TGTGTGCGTG GGGAGCGCCG CGTGCGGCTC CGCGCTGCCC GGCGGCTGTG AGCGCTGCGG GCGCGGCGCG GGGCTTTGTG CGCTCCGCAG TGTGCGCGAG GGGAGCGCGG CCGGGGGCGG TGCCCCGCGG TGCGGGGGGG GCTGCGAGGG GAACAAAGGC TGCGTGCGGG GTGTGTGCGT GGGGGGGTGA GCAGGGGGTG TGGGCGCGTC GGTCGGGCTG CAACCCCCCC TGCACCCCCC TCCCCGAGTT GCTGAGCACG GCCCGGCTTC GGGTGCGGGG CTCCGTACGG GGCGTGGCGC GGGGCTCGCC GTGCCGGGCG GGGGGTGGCG GCAGGTGGGG GTGCCGGGCG GGGCGGGGCC GCCTCGGGCC GGGGAGGGCT CGGGGGAGGG GCGCGGCGGC CCCCGGAGCG CCGGCGGCTG TCGAGGCGCG GCGAGCCGCA ACCATTGCCT TTTATGGTAA TCGTGCGAGA GGGCGCAAGG ACTTCCTTTG TCCCAAATCT GTGCGGAGCC GAAATCTGGG AGGCGCCGCC GCACCCCCTC TAGCGGGCGC GGGGCGAAGC GGTGCGGCGC CGGCAGGAAG GAAATGGGCG GGGAGGGCCT TCGTGCGTCG CCGCGCCGCC GTCCCCTTCT CCCTCTCCAG CCTCGGGGCT GTCCGCGGGG GGACGGCTGC CTTCGGGGGG GACGGGGCAG GGCGGGGTTC GGCTTCTGGC GTGTGACCGG CGGCTCTAGA GCCTCTGCTA ACCATGTTCA TGCCTTCTTC TTTTTCCTAC AGCTCCTGGG CAACGTGCTG GTTATTGTGC TGTCTCATCA TTTTGGCAAA GAATTAATTA AGTTTAAACG GCGCGCCGAT ATCTTAAGAT CTATGGATGC CAACGTAGTA TCATCTTCTA CTATTGCAAC GTATATAGAC GCTTTAGCGA AGAATGCTTC AGAATTAGAA CAGAGGTCTA CCGCATACGA AATAAATAAT GAATTGGAAC TAGTATTTAT TAAGCCGCCA TTAATTACTT TGACAAATGT AGTGAATATC TCTACGATTC AGGAATCGTT TATTCGATTT ACCGTTACTA ATAAGGAAGG TGTTAAAATT AGAACTAAGA TTCCATTATC TAAGGTACAT GGTCTAGATG TAAAAAATGT ACAGTTAGTA GATGCTATAG ATAACATAGT TTGGGAAAAG AAATCATTAG TGACGGAAAA TCGTCTTCAC AAAGAATGCT TGTTGAGACT ATCGACAGAG GAACGTTATA TATTTTTGGA TTACAAGAAA TATGGATCCT CTATCCGACT AGAATTAGTC AATCTTATTC AAGCAAAAAC AAAAAACTTT ACGATAGACT TTAAGCTAAA ATATTTTCTA GGATCCGGTG CCCAGTCTAA AAGTTCTTTA TTACACGCTA TTAATCATCC AAAGTCAAGG CCTAATACAT CTCTGGAAAT AGAATTCACA CCTAGAGACA ATGAAAAAGT TCCATATGAT GAACTAATAA AGGAATTGAC AACTCTATCA CGTCATATAT TTATGGCTTC TCCAGAGAAT GTAATTCTTT CTCCGCCTAT TAACGCGCCT ATAAAAACCT TTATGTTGCC TAAACAAGAT ATAGTAGGTT TGGATCTGGA AAATCTATAT GCCGTAACTA AGACTGACGG CATTCCTATA ACTATCAGAG TTACATCAAA CGGGTTGTAT TGTTATTTTA CACATCTTGG TTATATTATT AGATATCCTG TTAAGAGAAT AATAGATTCC GAAGTAGTAG TCTTTGGTGA GGCAGTTAAG GATAAGAACT GGACCGTATA TCTCATTAAG CTAATAGAGC CTGTGAATGC AATCAATGAT AGACTAGAAG AAAGTAAGTA TGTTGAATCT AAACTAGTGG ATATTTGTGA TCGGATAGTA TTCAAGTCAA AGAAATATGA AGGTCCGTTT ACTACAACTA GTGAAGTCGT CGATATGTTA TCTACATATT TACCAAAGCA ACCAGAAGGT GTTATTCTGT TCTATTCAAA GGGACCTAAA TCTAACATTG ATTTTAAAAT TAAAAAGGAA AATACTATAG ACCAAACTGC AAATGTAGTA TTTAGGTACA TGTCCAGTGA ACCAATTATC TTTGGAGAAT CGTCTATCTT TGTAGAGTAT AAGAAATTTA GCAACGATAA AGGCTTTCCT AAAGAATATG GTTCTGGTAA GATTGTGTTA TATAACGGCG TTAATTATCT AAATAATATC TATTGTTTGG AATATATTAA TACACATAAT GAAGTGGGTA TTAAGTCCGT GGTTGTACCT ATTAAGTTTA TAGCAGAATT CTTAGTTAAT GGAGAAATAC TTAAACCTAG AATTGATAAA ACCATGAAAT ATATTAACTC AGAAGATTAT TATGGAAATC AACATAATAT CATAGTCGAA CATTTAAGAG ATCAAAGCAT CAAAATAGGA GATATCTTTA ACGAGGATAA ACTATCGGAT GTGGGACATC AATACGCCAA TAATGATAAA TTTAGATTAA ATCCAGAAGT TAGTTATTTT ACGAATAAAC GAACTAGAGG ACCGTTGGGA ATTTTATCAA ACTACGTCAA GACTCTTCTT ATTTCTATGT ATTGTTCCAA AACATTTTTA GACGATTCCA ACAAACGAAA GGTATTGGCG ATTGATTTTG GAAACGGTGC TGACCTGGAA AAATACTTTT ATGGAGAGAT TGCGTTATTG GTAGCGACGG ATCCGGATGC TGATGCTATA GCTAGAGGAA ATGAAAGATA CAACAAATTA AACTCTGGAA TTAAAACCAA GTACTACAAA TTTGACTACA TTCAGGAAAC TATTCGATCC GATACATTTG TCTCTAGTGT CAGAGAAGTA TTCTATTTTG GAAAGTTTAA TATCATCGAC TGGCAGTTTG CTATCCATTA TTCTTTTCAT CCGAGACATT ATGCTACCGT CATGAATAAC TTATCCGAAC TAACTGCTTC TGGAGGCAAG GTATTAATCA CTACCATGGA CGGAGACAAA TTATCAAAAT TAACAGATAA AAAGACTTTT ATAATTCATA AGAATTTACC TAGTAGCGAA AACTATATGT CTGTAGAAAA AATAGCTGAT GATAGAATAG TGGTATATAA TCCATCAACA ATGTCTACTC CAATGACTGA ATACATTATC AAAAAGAACG ATATAGTCAG AGTGTTTAAC GAATACGGAT TTGTTCTTGT AGATAACGTT GATTTCGCTA CAATTATAGA ACGAAGTAAA AAGTTTATTA ATGGCGCATC TACAATGGAA GATAGACCGT CTACAAAAAA CTTTTTCGAA CTAAATAGAG GAGCCATTAA ATGTGAAGGT TTAGATGTCG AAGACTTACT TAGTTACTAT GTTGTTTATG TCTTTTCTAA GCGGTAAAGA TCTTCGAATT CACTCCTCAG GTGCAGGCTG CCTATCAGAA GGTGGTGGCT GGTGTGGCCA ATGCCCTGGC TCACAAATAC CACTGAGATC GATCTTTTTC CCTCTGCCAA AAATTATGGG GACATCATGA AGCCCCTTGA GCATCTGACT TCTGGCTAAT AAAGGAAATT TATTTTCATT GCAATAGTGT GTTGGAATTT TTTGTGTCTC TCACTCGGAA GGACATATGG GAGGGCAAAT CATTTAAAAC ATCAGAATGA GTATTTGGTT TAGAGTTTGG CAACATATGC CCATATGCTG GCTGCCATGA ACAAAGGTTG GCTATAAAGA GGTCATCAGT ATATGAAACA GCCCCCTGCT GTCCATTCCT TATTCCATAG AAAAGCCTTG ACTTGAGGTT AGATTTTTTT TATATTTTGT TTTGTGTTAT TTTTTTCTTT AACATCCCTA AAATTTTCCT TACATGTTTT ACTAGCCAGA TTTTTCCTCC TCTCCTGACT ACTCCCAGTC ATAGCTGTCC CTCTTCTCTT ATGGAGATCC CTCGACCTGC AGCCCAAGCT CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTTCCGCC CTTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTTGCATG CCTGCAGGTC GGCCGCCACG ACCGGTGCCG CCACCATCCC CTGACCCACG CCCCTGACCC CTCACAAGGA GACGACCTTC CATGACCGAG TACAAGCCCA CGGTGCGCCT CGCCACCCGC GACGACGTCC CCCGGGCCGT ACGCACCCTC GCCGCCGCGT TCGCCGACTA CCCCGCCACG CGCCACACCG TCGACCCGGA CCGCCACATC GAGCGGGTCA CCGAGCTGCA AGAACTCTTC CTCACGCGCG TCGGGCTCGA CATCGGCAAG GTGTGGGTCG CGGACGACGG CGCCGCGGTG GCGGTCTGGA CCACGCCGGA GAGCGTCGAA GCGGGGGCGG TGTTCGCCGA GATCGGCCCG CGCATGGCCG AGTTGAGCGG TTCCCGGCTG GCCGCGCAGC AACAGATGGA AGGCCTCCTG GCGCCGCACC GGCCCAAGGA GCCCGCGTGG TTCCTGGCCA CCGTCGGCGT CTCGCCCGAC CACCAGGGCA AGGGTCTGGG CAGCGCCGTC GTGCTCCCCG GAGTGGAGGC GGCCGAGCGC GCCGGGGTGC CCGCCTTCCT GGAGACCTCC GCGCCCCGCA ACCTCCCCTT CTACGAGCGG CTCGGCTTCA CCGTCACCGC CGACGTCGAG GTGCCCGAAG GACCGCGCAC CTGGTGCATG ACCCGCAAGC CCGGTGCCTG ACGCCCGCCC CACGACCCGC AGCGCCCGAC CGAAAGGAGC GCACGACCCC ATGGCTCCGA CCGAAGCCAC CCGGGGCGGC CCCGCCGACC CCGCACCCGC CCCCGAGGCC CACCGACTCT AGAGGATCAT AATCAGCCAT ACCACATTTG TAGAGGTTTT ACTTGCTTTA AAAAACCTCC CACACCTCCC CCTGAACCTG AAACATAAAA TGAATGCAAT TGTTGTTGTT AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT GGATCCGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGGGTC
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
