pMAD
$113.00
In stock
SKU
PL84113
金黄葡萄球温敏
100μg liquid plasmid(Instantly):$134
| name | pMAD | another name | apMAD |
| Insert Size (bp) | Plasmid host | Golden Staphylococcus | |
| Plasmid uses | Fragment type | ||
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | ||
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GAATTAATTC TCATGTTTGA CAGCTTATCA TCGATTCACA AAAAATAGGC ACACGAAAAA CAAGTTAAGG GATGCAGTTT ATGCATCCCT TAACTTACTT ATTAAATAAT TTATAGCTAT TGAAAAGAGA TAAGAATTGT TCAAAGCTAA TATTGTTTAA ATCGTCAATT CCTGCATGTT TTAAGGAATT GTTAAATTGA TTTTTTGTAA ATATTTTCTT GTATTCTTTG TTAACCCATT TCATAACGAA ATAATTATAC TTTTGTTTAT CTTTGTGTGA TATTCTTGAT TTTTTTCTAC TTAATCTGAT AAGTGAGCTA TTCACTTTAG GTTTAGGATG AAAATATTCT CTTGGAACCA TACTTAATAT AGAAATATCA ACTTCTGCCA TTAAAAGTAA TGCCAATGAG CGTTTTGTAT TTAATAATCT TTTAGCAAAC CCGTATTCCA CGATTAAATA AATCTCATTA GCTATACTAT CAAAAACAAT TTTGCGTATT ATATCCGTAC TTATGTTATA AGGTATATTA CCATATATTT TATAGGATTG GTTTTTAGGA AATTTAAACT GCAATATATC CTTGTTTAAA ACTTGGAAAT TATCGTGATC AACAAGTTTA TTTTCTGTAG TTTTGCATAA TTTATGGTCT ATTTCAATGG CAGTTACGAA ATTACACCTC TTTACTAATT CAAGGGTAAA ATGGCCTTTT CCTGAGCCGA TTTCAAAGAT ATTATCATGT TCATTTAATC TTATATTTGT CATTATTTTA TCTATATTAT GTTTTGAAGT AATAAAGTTT TGACTGTGTT TTATATTTTT CTCGTTCATT ATAACCCTCT TTAATTTGGT TATATGAATT TTGCTTATTA ACGATTCATT ATAACCACTT ATTTTTTGTT TGGTTGATAA TGAACTGTGC TGATTACAAA AATACTAAAA ATGCCCATAT TTTTTCCTCC TTATAAAATT AGTATAATTA TAGCACGAGC TCTGATAAAT ATGAACATGA TGAGTGATCG TTAAATTTAT ACTGCAATCG GATGCGATTA TTGAATAAAA GATATGAGAG ATTTATCTAA TTTCTTTTTT CTTGTAAAAA AAGAAAGTTC TTAAAGGTTT TATAGTTTTG GTCGTAGAGC ACACGGTTTA ACGACTTAAT TACGAAGTAA ATAAGTCTAG TGTGTTAGAC TTTATGAAAT CTATATACGT TTATATATAT TTATTATCCG GAGGTGTAGC ATGTCTCATT CAATTTTGAG GGTTGCCAGA GTTAAAGGAT CAAGTAATAC AAACGGGATA CAAAGACATA ATCAAAGAGA GAATAAAAAC TATAATAATA AAGACATAAA TCATGAGGAA ACATATAAAA ATTATGATTT GATTAACGCA CAAAATATAA AGTATAAAGA TAAAATTGAT GAAACGATTG ATGAGAATTA TTCAGGGAAA CGTAAAATTC GGTCAGATGC AATTCGACAT GTGGACGGAC TGGTTACAAG TGATAAAGAT TTCTTTGATG ATTTAAGCGG AGAAGAAATA GAACGATTTT TTAAAGATAG CTTGGAGTTT CTAGAAAATG AATACGGTAA GGAAAATATG CTGTATGCGA CTGTCCATCT GGATGAAAGA GTCCCACATA TGCACTTTGG TTTTGTCCCT TTAACAGAGG ACGGGAGATT GTCTGCAAAA GAACAGTTAG GCAACAAGAA AGACTTTACT CAATTACAAG ATAGATTTAA TGAGTATGTG AATGAGAAAG GTTATGAACT TGAAAGAGGC ACGTCCAAAG AGGTTACAGA ACGAGAACAT AAAGCGATGG ATCAGTACAA GAAAGATACT GTATTTCATA AACAGGAACT GCAAGAAGTT AAGGATGAGT TACAGAAGGC AAATAAGCAG TTACAGAGTG GAATAGAGCA TATGAGGTCT ACGAAACCCT TTGATTATGA AAATGAGCGT ACAGGTTTGT TCTCTGGACG TGAAGAGACT GGTAGAAAGA TATTAACTGC TGATGAATTT GAACGCCTGC AAGAAACAAT CTCTTCTGCA GAACGGATTG TTGATGATTA CGAAAATATT AAGAGCACAG ACTATTACAC AGAAAATCAA GAATTAAAAA AACGTAGAGA GAGTTTGAAA GAAGTAGTGA ATACATGGAA AGAGGGGTAT CACGAAAAAA GTAAAGAGGT TAATAAATTA AAGCGAGAGA ATGATAGTTT GAATGAGCAG TTGAATGTAT CAGAGAAATT TCAAGCTAGT ACAGTGACTT TATATCGTGC TGCGAGGGCG AATTTCCCTG GGTTTGAGAA AGGGTTTAAT AGGCTTAAAG AGAAATTCTT TAATGATTCC AAATTTGAGC GTGTGGGACA GTTTATGGAT GTTGTACAGG ATAATGTCCA GAAGGTCGAT AGAAAGCGTG AGAAACAGCG TACAGACGAT TTAGAGATGT AGAGGTACTT TTATGCCGAG AAAACTTTTT GCGTGTGACA GTCCTTAAAA TATACTTAGA GCGTAAGCGA AAGTAGTAGC GACAGCTATT AACTTTCGGT TGCAAAGCTC TAGGATTTTT AATGGACGCA GCGCATCACA CGCAAAAAGG AAATTGGAAT AAATGCGAAA TTTGAGATGT TAATTAAAGA CCTTTTTGAG GTCTTTTTTT CTTAGATTTT TGGGGTTATT TAGGGGAGAA AACATAGGGG GGTACTACGA CCTCCCCCCT AGGTGTCCAT TGTCCATTGT CCAAACAAAT AAATAAATAT TGGGTTTTTA ATGTTAAAAG GTTGTTTTTT ATGTTAAAGT GAAAAAAACA GATGTTGGGA GGTACAGTGA TAGTTGTAGA TAGAAAAGAA GAGAAAAAAG TTGCTGTTAC TTTAAGACTT ACAACAGAAG AAAATGAGAT ATTAAATAGA ATCAAAGAAA AATATAATAT TAGCAAATCA GATGCAACCG GTATTCTAAT AAAAAAATAT GCAAAGGAGG AATACGGTGC ATTTTAAACA AAAAAAGATA GACAGCACTG GCATGCTGCC TATCTATGAC TAAATTTTGT TAAGTGTATT AGCACCGTTA TTATATCATG AGCGAAAATG TAATAAAAGA AACTGAAAAC AAGAAAAATT CAAGAGGACG TAATTGGACA TTTGTTTTAT ATCCAGAATC AGCAAAAGCC GAGTGGTTAG AGTATTTAAA AGAGTTACAC ATTCAATTTG TAGTGTCTCC ATTACATGAT AGGGATACTG ATACAGAAGG TAGGATGAAA AAAGAGCATT ATCATATTCT AGTGATGTAT GAGGGTAATA AATCTTATGA ACAGATAAAA ATAATTAACA GAAGAATTGA ATGCGACTAT TCCGCAGATT GCAGGAAGTG TGAAAGGTCT TGTGAGATAT ATGCTTCACA TGGACGATCC TAATAAATTT AAATATCAAA AAGAAGATAT GATAGTTTAT GGCGGTGTAG ATGTTGATGA ATTATTAAAG AAAACAACAA CAGATAGATA TAAATTAATT AAAGAAATGA TTGAGTTTAT TGATGAACAA GGAATCGTAG AATTTAAGAG TTTAATGGAT TATGCAATGA AGTTTAAATT TGATGATTGG TTCCCGCTTT TATGTGATAA CTCGGCGTAT GTTATTCAAG AATATATAAA ATCAAATCGG TATAAATCTG ACCGATAGAT TTTGAATTTA GGTGTCACAA GACACTCTTT TTTCGCACCA GCGAAAACTG GTTTAAGCCG ACTGCGCAAA AGACATAATC GATAAGCTTT AATGCGGTAG TTTATCACAG TTAAATTGCT AACGCAGTCA GGCACCGTGT ATGAAATCTA ACAATGCGCT CATCGTCATC CTCGGCACCG TCACCCTGGA TGCTGTAGGC ATAGGCTTGG TTATGCCGGT ACTGCCGGGC CTCTTGCGGG ATCGAGGCCT TTAGCATATT ATGTTGCCAA CTGTCGGAAC GAGACTTCTC TAAACCTTCC CGGCTTCATC ATGCTCTCTT AACACTGCTA CATCAACCCC TTGAATCCTC AATTCACCTC CTCGAAAACA TTTCCCATCA ATCATATTCT GGTATATCTT ATCTTCTGGC AGTGACAGCG TCACTTCGTA ATCATTATGA TTGATGATAA TCAAATACTT CCATTCATCA GTCTCTCTTT GTTGCACCTC TACATTTTCA GCTACTTCAA GAATGGGATT AATATGATGT TTAGCGAAAA CCTGTTCTAA AAGCCTGCCT AAATAATTAC TATCTGGATA AGTACCGACG TAAATCCCCT CTCCTTTACC GTAGCAGTTA CGTGTAACCG CCGGAAGTCC TGCATACCAA TCCCCCTTAA ATGTCGCTAG AGGTTCTGCC CCTTCTAATC GGATTATGTC CGCCCACGTC GTACAATCAT ATTCCCCATC GTTACTATAT ATTTTGTTTA CCTTTGTTTC TGGGTATGGT ACAAATTCCT CAACAAAAAT CCCCAAAATA TCTCGCAGAG GACCAGGATA TCCGCCTAGA TGTACACGGT CATTTTCATC TACAATGCCA CTGAAGAAAC TGACAATCAA AGTGCCACCG TTAGCAACAA ATTGCCGTAA GTTTTCATCT TCTCCCTCTT TAACCATATA TAACATTGGA GCAATAACTA CTTTGTATTT TGTTAGATCA TCAGATGGCC TTACAAAATC GACAGCAATA TTACGTTTAT ATAATTCCCT ATAATAAGCT TCAACTATAG GAATATATCT TAGTTTATTA TGTGGTTTGG AACTTAGTTC GACAGCCCAC CAGTTTTCCC AATCAAAAAT GATCGCGACC TCTGCCTTGA TTCTAGATCC GACCAAACAA TCTAACTTTT TCAGCTCTTG CCCTAACTGT GTAACTTCCC TATAAATTCT ATTATTCTCG TTCAAAAAGT GGGGCACCAT TGCACCGTGG AATTTTTCAG CTCCTGCTCT ACTTTGACGC CACTGGAAAA ACATAATACC ATCTGCACCA CGGGCAATAG TTGCATAACT CCATAGACGC ATTACACCTG GCGGTTTTGG AACATTAATA TCGCGCCAGT TAACATGTGA GGTTACCTGC TCCATCAAAA TAAACGGTTG ACCTTTTCTT AAACTACGCA TAAGGTCATT CATCATGGCG TGCTGAATTG GCAAGCCCTC TCTGGGATCA GGATATGAGT CCCATGTCAC AATATCTACA TGCTGAGCCC ATTGAAAATA GTTTAACGGT TTGAATGAAC CCATGAAATT AGTTGATACT GGAATATCTG GTGTTACCTC ACGTAAAATT TCCTTTTCTG TTAAAAACAA CTTGAGAATT GAGTCATTCA TAAAACGGTA GTAATCAAGT TCTTGGGATG GATTAATAAA AGTTGGTGCC TTTCTAGGGG GATTAATTTC ATCCCAATGA TTGTATCGCT GTCCCCAAAA GTTTGTACCC CAACGTTCAT TTAATTCATC GATTGTTTTA TATCTTTCCT TTAGCCACTT TCTAAACGCG ACAGCACAAT TCTCACAAAA ACACTTGGAA ACGTGACATG CATACTCATT ATTAACATGC CACATTTTGA GTGCCGGATG ATTTTTATAC CGTTCTGCTA TAGCCCTCAC AAGTCTCTTT ATGTGCGTAA TTAATTGAGG ATGATTAGGA CAATAATGTT GTCTACTGCC AAACGAGAGA ATGACACCGC TTTCATCGAT CGGCAAAGAA TCTGGATACT TTTTTACAAA CCAAGCTGGA GTAGTTGCAG TCGCCGTCCC CAAGTTAATA TAAACACCGT GGTCATATAG TATATCTATA ACCTTGTCTA GCCATTCAAA GTCGAACACT CCATCAGACG GTTCGATCTT GCTCCAACTG AAAATCCCTA AAGATACTAA ATTCACCCCC GCTTTTTGCA TCAACTTAGC ATCTTCATAC CAAATTTCCT CTGGCCATTG CTCTGGGTTA TAATCTCCTC CGTAACAAAT TGAGGATAAC ACATTCATAA TTATTCCCCC TAGCTAATTT TCGTTTAATT ATAAATTAAG TTAAAATTTA GGTACCTACG TAGGATCGAT CCGATCCTTA ATTATATTAT AGTCCCAATA TAATCATTTA TCAACTCTTT TACACTTAAA TTTCCTAATA TTTGTAAATA TTTTTTTGAA TAAAAAAATC TAGCTAATGT TACGTTACAC ATTAACTAGA CAGATCTATC GATGCATGCC ATGGTACCCG GGAGCTCGAA TTCTAGAAGC TTCTGCAGAC GCGTCGACGT CATATGGATC CGATATCGCC CGACGCGAGG CTGGATGGCC TTCCCCATTA TGATTCTTCT CGCTTCCGGC GGCATCGGGA TGCCCGCGTT GCAGGCCATG CTGTCCAGGC AGGTAGATGA CGACCATCAG GGACAGCTTC AAGGATCGCT CGCGGCTCTT ACCAGCCTAA CTTCGATCAC TGGACCGCTG ATCGTCACGG CGATTTATGC CGCCTCGGCG AGCACATGGA ACGGGTTGGC ATGGATTGTA GGCGCCGCCC TATACCTTGT CTGCCTCCCC GCGTTGCGTC GCGGTGCATG GAGCCGGGCC ACCTCGACCT GAATGGAAGC CGGCGGCACC TCGCTAACGG ATTCACCACT CCAAGAATTG GAGCCAATCA ATTCTTGCGG AGAACTGTGA ATGCGCAAAC CAACCCTTGG CAGAACATAT CCATCGCGTC CGCCATCTCC AGCAGCCGCA CGCGGCGCAT CTCGGGCAGC GTTGGGTCCT GGCCACGGGT GCGCATGATC GTGCTCCTGT CGTTGAGGAC CCGGCTAGGC TGGCGGGGTT GCCTTACTGG TTAGCAGAAT GAATCACCGA TACGCGAGCG AACGTGAAGC GACTGCTGCT GCAAAACGTC TGCGACCTGA GCAACAACAT GAATGGTCTT CGGTTTCCGT GTTTCGTAAA GTCTGGAAAC GCGGAAGTCA GCGCCCTGCA CCATTATGTT CCGGATCTGC ATCGCAGGAT GCTGCTGGCT ACCCTGTGGA ACACCTACAT CTGTATTAAC GAAGCGCTGG CATTGACCCT GAGTGATTTT TCTCTGGTCC CGCCGCATCC ATACCGCCAG TTGTTTACCC TCACAACGTT CCAGTAACCG GGCATGTTCA TCATCAGTAA CCCGTATCGT GAGCATCCTC TCTCGTTTCA TCGGTATCAT TACCCCCATG AACAGAAATT CCCCCTTACA CGGAGGCATC AAGTGACCAA ACAGGAAAAA ACCGCCCTTA ACATGGCCCG CTTTATCAGA AGCCAGACAT TAACGCTTCT GGAGAAACTC AACGAGCTGG ACGCGGATGA ACAGGCAGAC ATCTGTGAAT CGCTTCACGA CCACGCTGAT GAGCTTTACC GCAGCTGCCT CGCGCGTTTC GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT CGGGGCGCAG CCATGACCCA GTCACGTAGC GATAGCGGAG TGTATACTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTGCAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAACAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTCTAA GAAACCATTA TTATCATGAC ATTAACCTAT AAAAATAGGC GTATCACGAG GCCCTTTCGT CTTCAA
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