pECTO
金黄葡萄球染体异位互补
100μg liquid plasmid(Instantly):$120
金黄葡萄球染体异位互补
100μg liquid plasmid(Instantly):$120
| name | pECTO | another name | apECTO |
| Insert Size (bp) | Plasmid host | Golden Staphylococcus | |
| Plasmid uses | Fragment type | ||
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | ||
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GAATTAATTC TCATGTTTGA CAGCTTATCA TCGATTCACA AAAAATAGGC ACACGAAAAA CAAGTTAAGG GATGCAGTTT ATGCATCCCT TAACTTACTT ATTAAATAAT TTATAGCTAT TGAAAAGAGA TAAGAATTGT TCAAAGCTAA TATTGTTTAA ATCGTCAATT CCTGCATGTT TTAAGGAATT GTTAAATTGA TTTTTTGTAA ATATTTTCTT GTATTCTTTG TTAACCCATT TCATAACGAA ATAATTATAC TTTTGTTTAT CTTTGTGTGA TATTCTTGAT TTTTTTCTAC TTAATCTGAT AAGTGAGCTA TTCACTTTAG GTTTAGGATG AAAATATTCT CTTGGAACCA TACTTAATAT AGAAATATCA ACTTCTGCCA TTAAAAGTAA TGCCAATGAG CGTTTTGTAT TTAATAATCT TTTAGCAAAC CCGTATTCCA CGATTAAATA AATCTCATTA GCTATACTAT CAAAAACAAT TTTGCGTATT ATATCCGTAC TTATGTTATA AGGTATATTA CCATATATTT TATAGGATTG GTTTTTAGGA AATTTAAACT GCAATATATC CTTGTTTAAA ACTTGGAAAT TATCGTGATC AACAAGTTTA TTTTCTGTAG TTTTGCATAA TTTATGGTCT ATTTCAATGG CAGTTACGAA ATTACACCTC TTTACTAATT CAAGGGTAAA ATGGCCTTTT CCTGAGCCGA TTTCAAAGAT ATTATCATGT TCATTTAATC TTATATTTGT CATTATTTTA TCTATATTAT GTTTTGAAGT AATAAAGTTT TGACTGTGTT TTATATTTTT CTCGTTCATT ATAACCCTCT TTAATTTGGT TATATGAATT TTGCTTATTA ACGATTCATT ATAACCACTT ATTTTTTGTT TGGTTGATAA TGAACTGTGC TGATTACAAA AATACTAAAA ATGCCCATAT TTTTTCCTCC TTATAAAATT AGTATAATTA TAGCACGAGC TCTGATAAAT ATGAACATGA TGAGTGATCG TTAAATTTAT ACTGCAATCG GATGCGATTA TTGAATAAAA GATATGAGAG ATTTATCTAA TTTCTTTTTT CTTGTAAAAA AAGAAAGTTC TTAAAGGTTT TATAGTTTTG GTCGTAGAGC ACACGGTTTA ACGACTTAAT TACGAAGTAA ATAAGTCTAG TGTGTTAGAC TTTATGAAAT CTATATACGT TTATATATAT TTATTATCCG GAGGTGTAGC ATGTCTCATT CAATTTTGAG GGTTGCCAGA GTTAAAGGAT CAAGTAATAC AAACGGGATA CAAAGACATA ATCAAAGAGA GAATAAAAAC TATAATAATA AAGACATAAA TCATGAGGAA ACATATAAAA ATTATGATTT GATTAACGCA CAAAATATAA AGTATAAAGA TAAAATTGAT GAAACGATTG ATGAGAATTA TTCAGGGAAA CGTAAAATTC GGTCAGATGC AATTCGACAT GTGGACGGAC TGGTTACAAG TGATAAAGAT TTCTTTGATG ATTTAAGCGG AGAAGAAATA GAACGATTTT TTAAAGATAG CTTGGAGTTT CTAGAAAATG AATACGGTAA GGAAAATATG CTGTATGCGA CTGTCCATCT GGATGAAAGA GTCCCACATA TGCACTTTGG TTTTGTCCCT TTAACAGAGG ACGGGAGATT GTCTGCAAAA GAACAGTTAG GCAACAAGAA AGACTTTACT CAATTACAAG ATAGATTTAA TGAGTATGTG AATGAGAAAG GTTATGAACT TGAAAGAGGC ACGTCCAAAG AGGTTACAGA ACGAGAACAT AAAGCGATGG ATCAGTACAA GAAAGATACT GTATTTCATA AACAGGAACT GCAAGAAGTT AAGGATGAGT TACAGAAGGC AAATAAGCAG TTACAGAGTG GAATAGAGCA TATGAGGTCT ACGAAACCCT TTGATTATGA AAATGAGCGT ACAGGTTTGT TCTCTGGACG TGAAGAGACT GGTAGAAAGA TATTAACTGC TGATGAATTT GAACGCCTGC AAGAAACAAT CTCTTCTGCA GAACGGATTG TTGATGATTA CGAAAATATT AAGAGCACAG ACTATTACAC AGAAAATCAA GAATTAAAAA AACGTAGAGA GAGTTTGAAA GAAGTAGTGA ATACATGGAA AGAGGGGTAT CACGAAAAAA GTAAAGAGGT TAATAAATTA AAGCGAGAGA ATGATAGTTT GAATGAGCAG TTGAATGTAT CAGAGAAATT TCAAGATAGT ACAGTGACTT TATATCGTGC TGCGAGGGCG AATTTCCCTG GGTTTGAGAA AGGGTTTAAT AGGCTTAAAG AGAAATTCTT TAATGATTCC AAATTCGAGC GTGTGGGACA GTTTATGGAT GTTGTACAGG ATAATGTCCA GAAGGTCGAT AGAAAGCGTG AGAAACAGCG TACAGACGAT TTAGAGATGT AGAGGTACTT TTATGCCGAG AAAACTTTTT GCGTGTGACA GTCCTTAAAA TATACTTAGA GCGTAAGCGA AAGTAGTAGC GACAGCTATT AACTTTCGGT TGCAAAGCTC TAGGATTTTT AATGGACGCA GCGCATCACA CGCAAAAAGG AAATTGGAAT AAATGCGAAA TTTGAGATGT TAATTAAAGA CCTTTTTGAG GTCTTTTTTT CTTAGATTTT TGGGGTTATT TAGGGGAGAA AACATAGGGG GGTACTACGA CCTCCCCCCT AGGTGTCCAT TGTCCATTGT CCAAACAAAT AAATAAATAT TGGGTTTTTA ATGTTAAAAG GTTGTTTTTT ATGTTAAAGT GAAAAAAACA GATGTTGGGA GGTACAGTGA TGGTTGTAGA TAGAAAAGAA GAGAAAAAAG TTGCTGTTAC TTTAAGACTT ACAACAGAAG AAAATGAGAT ATTAAATAGA ATCAAAGAAA AATATAATAT TAGCAAATCA GATGCAACCG GTATTCTAAT AAAAAAATAT GCAAAGGAGG AATACGGTGC ATTTTAAACA AAAAAAGATA GACAGCACTG GCATGCTGCC TATCTATGAC TAAATTTTGT TAAATGTATT AGCACCGTTA TTATATCATG AGCGAAAATG TAATAAAAGA AACTGAAAAC AAGAAAAATT CAAGAGGACG TAATTGGACA TTTGTTTTAT ATCCAGAATC AGCAAAAGCC GAGTGGTTAG AGTATTTAAA AGAGTTACAC ATTCAATTTG TAGTGTCTCC ATTACATGAT AGGGATACTG ATACAGAAGA TAGGATGAAA AAAGAGCATT ATCATATTCT AGTGATGTAT GAGGGTAATA AATCTTATGA ACAGATAAAA ATAATTACAG AAGAATTGAA TGCGACTATT CCGCAGATTG CAGGAAGTGT GAAAGGTCTT GTGAGATATA TGCTTCACAT GGACGATCCT AATAAATTTA AATATCAAAA AGAAGATATG ATAGTTTATG GCGGTGTAGA TGTTGATGAA TTATTAAAGA AAACAACAAC AGATAGATAT AAATTAATTA AAGAAATGAT TGAGTTTATT GATGAACAAG GAATCGTAGA ATTTAAGAGT TTAATGGATT ATGCAATGAA GTTTAAATTT GATGATTGGT TCCCGCTTTT ATGTGATAAC TCGGCGTATG TTATTCAAGA ATATATAAAA TCAAATCGGT ATAAATCTGA CCGATAGATT TTGAATTTAA GAGTGTCACA AGACACTCTT TTTTCGCACC AACGAAAACT GGTTTAAGCC GACTGCGCAA AAGACATAAT CGATAAGCTT TAATGCGGTA GTTTATCACA GTTAAATTGC TAACGCAGTC AGGCACCGTG TATGAAATCT AACAATGCGC TCATCGTCAT CCTCGGCACC GTCACCCTGG ATGCTGTAGG CATAGGCTTG GTTATGCCGG TACTGCCGGG CCTCTTGCGG GATCGAGGCC TTTAGCATAT TATGTTGCCA ACTGTCGGAA CGAGACTTCT CTAAACCTTC CCGGCTTCAT CATGCTCTCT TAACACTGCT ACATCAACCC CTTGAATCCT CAATTCACCT CCTCGAAAAC ATTTCCCATC AATCATATTC TGGTATATCT TATCTTCTGG CAGTGACAGC GTCACTTCGT AATCATTATG ATTGATGATA ATCAAATACT TCCATTCATC AGTCTCTCTT TGTTGCACCT CTACATTTTC AGCTACTTCA AGAATGGGAT TAATATGATG TTTAGCGAAA ACCTGTTCTA AAAGCCTGCC TAAATAATTA CTATCTGGAT AAGTACCGAC GTAAATCCCC TCTCCTTTAC CGTAGCAGTT ACGTGTAACC GCCGGAAGTC CTGCATACCA ATCCCCCTTA AATGTCGCTA GAGGTTCTGC CCCTTCTAAT CGGATTATGT CCGCCCACGT CGTACAATCA TATTCCCCAT CGTTACTATA TATTTTGTTT ACCTTTGTTT CTGGGTATGG TACAAATTCC TCAACAAAAA TCCCCAAAAT ATCTCGCAGA GGACCAGGAT ATCCGCCTAG ATGTACACGG TCATTTTCAT CTACAATGCC ACTGAAGAAA CTGACAATCA AAGTGCCACC GTTAGCAACA AATTGCCGTA AGTTTTCATC TTCTCCCTCT TTAACCATAT ATAACATTGG AGCAATAACT ACTTTGTATT TTGTTAGATC ATCAGATGGC CTTACAAAAT CGACAGCAAT ATTACGTTTA TATAATTCCC TATAATAAGC TTCAACTATA GGAATATATC TTAGTTTATT ATGTGGTTTG GAACTTAGTT CGACAGCCCA CCAGTTTTCC CAATCAAAAA TGATCGCGAC CTCTGCCTTG ATTCTAGATC CGACCAAACA ATCTAACTTT TTCAGCTCTT GCCCTAACTG TGTAACTTCC CTATAAATTC TATTATTCTC GTTCAAAAAG TGGGGCACCA TTGCACCGTG GAATTTTTCA GCTCCTGCTC TACTTTGACG CCACTGGAAA AACATAATAC CATCTGCACC ACGGGCAATA GTTGCATAAC TCCATAGACG CATTACACCT GGCGGTTTTG GAACATTAAT ATCGCGCCAG TTAACATGTG AGGTTACCTG CTCCATCAAA ATAAACGGTT GACCTTTTCT TAAACTACGC ATAAGGTCAT TCATCATGGC GTGCTGAATT GGCAAGCCCT CTCTGGGATC AGGATATGAG TCCCATGTCA CAATATCTAC ATGCTGAGCC CATTGAAAAT AGTTTAACGG TTTGAATGAA CCCATGAAAT TAGTTGATAC TGGAATATCT GGTGTTACCT CACGTAAAAT TTCCTTTTCT GTTAAAAACA ACTTGAGAAT TGAGTCATTC ATAAAACGGT AGTAATCAAG TTCTTGGGAT GGATTAATAA AAGTTGGTGC CTTTCTAGGG GGATTAATTT CATCCCAATG ATTGTATCGC TGTCCCCAAA AGTTTGTACC CCAACGTTCA TTTAATTCAT CGATTGTTTT ATATCTTTCC TTTAGCCACT TTCTAAACGC GACAGCACAA TTCTCACAAA AACACTTGGA AACGTGACAT GCATACTCAT TATTAACATG CCACATTTTG AGTGCCGGAT GATTTTTATA CCGTTCTGCT ATAGCCCTCA CAAGTCTCTT TATGTGCGTA ATTAATTGAG GATGATTAGG ACAATAATGT TGTCTACTGC CAAACGAGAG AATGACACCG CTTTCATCGA TCGGCAAAGA ATCTGGATAC TTTTTTACAA ACCAAGCTGG AGTAGTTGCA GTCGCCGTCC CCAAGTTAAT ATAAACACCG TGGTCATATA GTATATCTAT AACCTTGTCT AGCCATTCAA AGTCGAACAC TCCATCAGAC GGTTCGATCT TGCTCCAACT GAAAATCCCT AAAGATACTA AATTCACCCC CGCTTTTTGC ATCAACTTAG CATCTTCATA CCAAATTTCC TCTGGCCATT GCTCTGGGTT ATAATCTCCT CCGTAACAAA TTGAGGATAA CACATTCATA ATTATTCCCC CTAGCTAATT TTCGTTTAAT TATAAATTAA GTTAAAATTT AGGTACCTAC GTAGGATCGA TCCGACCTTA ATTATATTAT AGTCCCAATA TAATCATTTA TCAACTCTTT TACACTTAAA TTTCCTAATA TTTGTAAATA TTTTTTTGAA TAAAAAAATC TAGCTAATGT TACGTTACAC ATTAACTAGA CAGATCTATC GATGCATGCC ATGGTACCCG GGAGCTCGAA TTCGAGACCG CTAGCGCAGT TTGATCAATT AGGCCGTGAA AACTATTATA TGTATAAAAA GTTTGGTGTT TTACCAGAAA TGGAATACGA AATGGAAGAA GTTAAAGAAA TCGAGCAATA TATTAAAGAG CAAGATGAAG CTGAACTATA GGGGCGATAA CATGACTTTC GAAGAAAAGC TAAGTCAAAT GTACAATGAA ATTGCAAATG AAATCAGTGG AATGATACCA GTAGAATGGG AAAAAGTATA TACAATTGCC TACGTAGATG ATGAAGGTGG AGAGGTTGTT TTTAATTATA CTAAACCAGG AAGTGAAGAT TTGAATTATT ATTCAGATAT TCCTAAAGAT TGCAATGTCT CAAAAGATAT TTTTAAGAAT TCATGGTTTA AAGTTTATCG AATGTTTGAT GAGTTAAGAG AAACTTTTAA AAAAGAAGAT TTAGAACCGT GGACATCATG TGAATTTGAC TTTACAAGAA AGGGAAATTT AAAAGTATCA TTTGATTATA TAGATTGGAT TAAATTAGGT TTTGGCCCAT CAGGAAAGGA AAACTACTAT ATGTACAAAA AATTTGGTAT TTTACCAGAT ATGGAATATG AAATGGAAGA AATTCGAGCA GTAGAGAAGT ATGTTAAAGA GCAAGAGTAG CAGACATGTT ATAAAAGACT GTGCAAAATC ACCCTCGTTT TACATTTGAT TCAAAGAAGA AGGTAAAAGA TAAGATTATT TGCAACTTAA AAAGTCAATT AGCTTATCGG TATCCATACA TCATGGATAA ATGAGTTCAA CTAATTAACA AATCACGATA TAAATTTATC GATTGATAAG TCGACGTCTC CCCATGCGAG AGTAGGGAAC TGCCAGGCAT CAAATAAAAC GAAAGGCTCA GTCGGAAGAC TGGGCCTTTC GTTTTATCTG TTGTTTGTCG GTGAACGCTC TCCTGAGTAG GATTGTCATT AATTGAAAAG GTTATTGTAG TGTATTTTAT AAAGTTGATG TACATCGACT TATTTTTTAA AACTTATATC AAAAAAATAT GAGAAAAGTA TATACTATTA AAATGTTTTT TACTATAATT ATAAATAGTT AAATATTTGG GAATTAAATA AATATATAGG AGGCAATTTT TAGAGATACA TAGCAATGTA AAGGTATCAA ATAGTTATTT CATAATTTAT TATGATGACA TAATTTTCAG TAGATAGTTG TAAATTAACA CTGAAATAGA TGTTTATATT AAGAAGACTT TAATATCATA ATTAATCAAA ATCTTGTGTC AAACATTTAA ACGAATTTTA AGTTGATAAC AATTGGCAAA ACATTGTTCA AACATCACAA TGATAAAGCA TATTATCAGT ATTGTAGTGT GTGGAAAATG ACAGCCATCT AAGGAGAAAA ATGATGAAAA GAATATTGGT AGTATTTTTA ATGTTAGCAA TTATATTGGC AGGTTGTTCT AATAAAGGTG AAAAGTATCA AAAAGATATT GATAAAGTGT ACAAAGAACA GAATCAAATG AATAAAATTG CCTCGAAAGT ACAAAACACT ATTAAAACAG ACATTAAACA AGAAGACAGT AATACACATG TTTATAAAGA TGGTAAAGTC ATTGTTATTG GTATTCAATT ATATAAAGAT CGTGAAAAAA TGTATTATTT CGCATATGAA ATAAAAGATG GTAAGGCAGA GATTAATAGA GAAATAGACC CAATTAAGTA TATGAAAGAC CATAAAGCAG ATTATGAAGA TGAAGGGTCT CGGATCCGAT ATCGCCCGAC GCGAGGCTGG ATGGCCTTCC CCATTATGAT TCTTCTCGCT TCCGGCGGCA TCGGGATGCC CGCGTTGCAG GCCATGCTGT CCAGGCAGGT AGATGACGAC CATCAGGGAC AGCTTCAAGG ATCGCTCGCG GCTCTTACCA GCCTAACTTC GATCATTGGA CCGCTGATCG TCACGGCGAT TTATGCCGCC TCGGCGAGCA CATGGAACGG GTTGGCATGG ATTGTAGGCG CCGCCCTATA CCTTGTCTGC CTCCCCGCGT TGCGTCGCGG TGCATGGAGC CGGGCCACCT CGACCTGAAT GGAAGCCGGC GGCACCTCGC TAACGGATTC ACCACTCCAA GAATTGGAGC CAATCAATTC TTGCGGAGAA CTGTGAATGC GCAAACCAAC CCTTGGCAGA ACATATCCAT CGCGTCCGCC ATCTCCAGCA GCCGCACGCG GCGCATCTCG GGCAGCGTTG GGTCCTGGCC ACGGGTGCGC ATGATCGTGC TCCTGTCGTT GAGGACCCGG CTAGGCTGGC GGGGTTGCCT TACTGGTTAG CAGAATGAAT CACCGATACG CGAGCGAACG TGAAGCGACT GCTGCTGCAA AACGTCTGCG ACCTGAGCAA CAACATGAAT GGTCTTCGGT TTCCGTGTTT CGTAAAGTCT GGAAACGCGG AAGTCAGCGC CCTGCACCAT TATGTTCCGG ATCTGCATCG CAGGATGCTG CTGGCTACCC TGTGGAACAC CTACATCTGT ATTAACGAAG CGCTGGCATT GACCCTGAGT GATTTTTCTC TGGTCCCGCC GCATCCATAC CGCCAGTTGT TTACCCTCAC AACGTTCCAG TAACCGGGCA TGTTCATCAT CAGTAACCCG TATCGTGAGC ATCCTCTCTC GTTTCATCGG TATCATTACC CCCATGAACA GAAATCCCCC TTACACGGAG GCATCAGTGA CCAAACAGGA AAAAACCGCC CTTAACATGG CCCGCTTTAT CAGAAGCCAG ACATTAACGC TTCTGGAGAA ACTCAACGAG CTGGACGCGG ATGAACAGGC AGACATCTGT GAATCGCTTC ACGACCACGC TGATGAGCTT TACCGCAGCT GCCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC GCAGCCATGA CCCAGTCACG TAGCGATAGC GGAGTGTATA CTGGCTTAAC TATGCGGCAT CAGAGCAGAT TGTACTGAGA GTGCACCATA TGCGGTGTGA AATACCGCAC AGATGCGTAA GGAGAAAATA CCGCATCAGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGATC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ACACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTTCAA