pSV40-Acss2-m-T736C
$99.00
In stock
SKU
PL83999
BC051432.1;1110017C11Rik;ACAS;AceCS1;Acs1
100μg liquid plasmid(Instantly):$120
| name | pSV40-Acss2-m-T736C | another name | apSV40-Acss2-m-T736C |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Gene template | Fragment type | cDNA |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | T7：TAATACGACTCACTATAGGG | |
| 3′ sequencing primer | pGEX-3'：CCGGGAGCTGCATGTGTCAGAGG | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GGTACCTTCT GAGGCGGAAA GAACCAGCCG GATCCCTCGA GGGATCCAGA CATGATAAGA TACATTGATG AGTTTGGACA AACCACAACT AGAATGCAGT GAAAAAAATG CTTTATTTGT GAAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG GCTGATTATG ATCCGGCTGC CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCGC AGCCATGACC CAGTCACGTA GCGATAGCGG AGTGTATACT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTAAATTGT AAGCGTTAAT ATTTTGTTAA AATTCGCGTT AAATTTTTGT TAAATCAGCT CATTTTTTAA CCAATAGGCC GAAATCGGCA AAATCCCTTA TAAATCAAAA GAATAGACCG AGATAGGGTT GAGTGTTGTT CCAGTTTGGA ACAAGAGTCC ACTATTAAAG AACGTGGACT CCAACGTCAA AGGGCGAAAA ACCGTCTATC AGGGCGATGG CCCACTACGT GAACCATCAC CCTAATCAAG TTTTTTGGGG TCGAGGTGCC GTAAAGCACT AAATCGGAAC CCTAAAGGGA GCCCCCGATT TAGAGCTTGA CGGGGAAAGC CGGCGAACGT GGCGAGAAAG GAAGGGAAGA AAGCGAAAGG AGCGGGCGCT AGGGCGCTGG CAAGTGTAGC GGTCACGCTG CGCGTAACCA CCACACCCGC CGCGCTTAAT GCGCCGCTAC AGGGCGCGTC CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG CCTCTTCGCT ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG TAACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGAG CGCGTCGACG GTATCGATAA GCTTGGCTGT GGAATGTGTG TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCGGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCTCGAGGAA CTGAAAAACC AGAAAGTTAA CTGGTAAGTT TAGTCTTTTT GTCTTTTATT TCAGGTCCCG GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGTTCGAA ATGACCGACC AAGCGACGCC CAACCTGCCG GGATCCAGAC ATGATAAGAT ACATTGATGA GTTTGGACAA ACCACAACTA GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT TTATTTGTAA CCATTATAAG CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT ATGTTTCAGG TTCAGGGGGA GGTGTGGGAG GTTTTTTAGC TTGGCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC TCGAGGAACT GAAAAACCAG AAAGTTAACT GGTAAGTTTA GTCTTTTTGT CTTTTATTTC AGGTCCCGGA TCCGGTGGTG GTGCAAATCA AAGAACTGCT CCTCAGTGGA TGTTGCCTTT ACTTCTAGGC CTGTACGGAA GTGTTACTTC TGCTCTAAAA GCTGCTGCAG GAGCTCGGAC CGGGCCCTTA GGACGCGTAA TACGACTCAC TATAGGGAAT TCGACGTCTA GATCCCCGGG GTTTTCCCCG CCCCGGCACC CGCAGCGACC GCAACGGCGG ACAAGGTGTT CGGAACTTGA TGCGATGGGG CTTCCCGAGG AGCGGCGCAA GAGCGGCAGT GGAAGCCGGG CTCGTGAGGA GACCGGCGCC GAGGGCCGGG TGCGGGGTTG GTCCCCGCCG CCGGAGGTCA GACGCTCGGC GCACGTCCCC TCTCTGCAGC GCTACCGCGA GCTGCACCGG CGTTCTGTGG AGGAGCCACG GGAGTTTTGG GGAAACATTG CCAAGGAATT TTACTGGAAA ACTGCATGCC CTGGCCCATT CCTCCAGTAC AACTTTGATG TGACTAAAGG GAAAATATTC ACTGAGTGGA TGAAAGGAGC AACTACAAAC ATCTGCTACA ACGTGCTGGA TCGAAATGTC CATGAGAAAA AACTTGGCGA CAAAGTTGCT TTTTACTGGG AGGGCAATGA GCCAGGGGAG ACCACCAAGA TCACATACCG TGAACTCCTG GTCCAGGTGT GTCAGTTCAG CAATGTTCTC CGTAAACAGG GCATTCAGAA GGGTGACCGA GTGGCCATCT ACATGCCTAT GATCTTGGAA CTTGTGGTGG CTATGCTGGC ATGTGCTCGC CTTGGAGCTT TGCACTCCAT TGTGTTTGCA GGCTTCTCTG CAGAGTCTCT CTGTGAAAGG ATCTTGGATT CCAGTTGCTG CCTGCTCATC ACTACAGATG CCTTCTACAG GGGGGAAAAA CTTGTGAACC TGAAGGAGCT GGCTGATGAG TCCTTGGAGA AGTGCCGAGA GAAGGGCTTC CCAGTGAGAT GCTGCATTGT GGTCAAACAT CTGGGGCGGG CAGAGCTGGG CATGAATGAC TCCCCCAGCC AGTCCCCACC AGTTAAGAGG CCATGTCCAG ATGTCCAGAT CTGCTGGAAC GAAGGGGTTG ACTTATGGTG GCATGAACTC ATGCAGCAGG CAGGAGACGA GTGTGAGCCT GAGTGGTGTG ATGCTGAGGA CCCACTCTTC ATCTTGTACA CCAGTGGCTC CACAGGCAAA CCTAAGGGTG TGGTGCACAC AATTGGAGGC TATATGCTCT ATGTGGCTAC AACTTTCAAG TATGTGTTTG ATTTCCACCC GGAAGATGTG TTCTGGTGCA CAGCAGACAT CGGCTGGATC ACTGGTCATT CCTATGTCAC CTATGGGCCA CTGGCTAATG GTGCCACTAG TGTTTTGTTT GAGGGGATCC CCACATACCC AGATGAAGGG CGCTTGTGGA GCATTGTGGA CAAATACAAG GTGACCAAGT TCTACACGGC ACCAACAGCC ATCCGGATGC TCATGAAGTT TGGAGATGAT CCTGTCACCA AGCATAGCCG GGCATCCCTG CAGGTGCTGG GCACAGTAGG TGAACCCATC AACCCTGAAG CCTGGCTATG GTACCACCGG GTAGTAGGTT CCCAGCGCTG CCCCATTGTA GACACCTTCT GGCAAACAGA AACAGGTGGC CATATGCTGA CCCCTCTCCC TGGCGCCACA CCCATGAAAC CTGGTTCTGC TTCTTTCCCA TTCTTCGGTG TAGCGCCTGC AATCCTGAAT GAGTCCGGGG AGGAGCTGGA AGGGGAAGCT GAAGGTTATC TGGTGTTCAA GCAGCCCTGG CCAGGGATCA TGCGCACAGT CTATGGGAAC CACACACGGT TTGAGACCAC CTACTTTAAG AAGTTCCCTG GCTACTATGT GACCGGAGAT GGCTGCCGAC GGGATCAGGA TGGCTATTAC TGGATCACGG GCAGGATTGA TGACATGCTC AATGTGTCTG GACATCTCCT GAGTACAGCA GAGGTGGAAT CGGCACTTGT GGAACACGAG GCTGTCGCAG AGGCAGCTGT GGTGGGCCAC CCTCATCCTG TGAAGGGCGA ATGCCTCTAC TGCTTTGTTA CCTTGTGTGA TGGCCATACC TTCAGCCCCA CACTCACAGA GGAACTCAAG AAGCAGATTA GAGAAAAGAT TGGCCCCATT GCCACACCAG ACTACATCCA GAATGCACCT GGCTTGCCTA AAACACGCTC AGGGAAAATC ATGAGGCGAG TTCTCCGGAA GATTGCTCAG AATGACCATG ACCTGGGGGA TACATCTACG GTGGCTGACC CATCTGTCAT CAACCATCTC TTCAGTCACC GCTGCCTGAC CACCCAGTGA ACAAGACTCT TGTCCAGGAT TTCCCTGTGC TTGGCTCTCC AAATTTCTGC CCATCCATCC TGCCCCTCAC ACAGTAGTAC TAGTTGACAG TACTAGTTGG TTGAAACTAG GGAGACAGTT ACCTAGACTA CCAGGTAGAA GGGACAGGGG TCAGGTCAAC CTCACACTGA TATGCTCCTC GACTAGAGAG GTCTCCAAGC CCAGAACAGA GACTTGAACA CCATGTCCTT AACCAAAGTC AGCTCTTCAG AGTGTAAATG AGGGTCTGAC TGGAAGCAGG GCAGCAGCTG TAATTCTTCC AGCTCTCTCC AAAAGCACCA GTTCTTATCT TGGGCCTGCA CTAGGCTTTA GAAATGACAG TTTGTGAGTC TATGAATAAT TATTTTTTTA AAAACTTTGT TGTCTCTGTT CTGAGTCTGA ATTCCTTGCA GATGGTAGCA TTGTCTACCA GTTGGTTCAG TCTCCATAGG ACTTTCATAT CATCTGCTAC ATTGAAAGAA TAAGATATAC TGATCCTAAA ACCAAGGGAA TTAACATTCC TAATCCTGCA CTATGAACAG AACCCTGCCC ATGAGAGGCA CTTGTAGGTG AAACTCTCTT TTCTTGTTCT TTTTGTGCTC AAGACCCACT GTTATAGGAA TAAAGTTTAT GAGCTCAAGA ACAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAGCGGCCGC CCTTTAGTGA GGGTTAATTT AAATCGTACG TCGCGATTAA TTAACCGC
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