pBin19
$128.00
In stock
SKU
PL83970
100μg liquid plasmid(Instantly):$149
| name | pBin19 | another name | apBin19 |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | Replicon | ||
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CCGGGCTGGT TGCCCTCGCC GCTGGGCTGG CGGCCGTCTA TGGCCCTGCA AACGCGCCAG AAACGCCGTC GAAGCCGTGT GCGAGACACC GCGGCCGCCG GCGTTGTGGA TACCTCGCGG AAAACTTGGC CCTCACTGAC AGATGAGGGG CGGACGTTGA CACTTGAGGG GCCGACTCAC CCGGCGCGGC GTTGACAGAT GAGGGGCAGG CTCGATTTCG GCCGGCGACG TGGAGCTGGC CAGCCTCGCA AATCGGCGAA AACGCCTGAT TTTACGCGAG TTTCCCACAG ATGATGTGGA CAAGCCTGGG GATAAGTGCC CTGCGGTATT GACACTTGAG GGGCGCGACT ACTGACAGAT GAGGGGCGCG ATCCTTGACA CTTGAGGGGC AGAGTGCTGA CAGATGAGGG GCGCACCTAT TGACATTTGA GGGGCTGTCC ACAGGCAGAA AATCCAGCAT TTGCAAGGGT TTCCGCCCGT TTTTCGGCCA CCGCTAACCT GTCTTTTAAC CTGCTTTTAA ACCAATATTT ATAAACCTTG TTTTTAACCA GGGCTGCGCC CTGTGCGCGT GACCGCGCAC GCCGAAGGGG GGTGCCCCCC CTTCTCGAAC CCTCCCGGCC CGCTAACGCG GGCCTCCCAT CCCCCCAGGG GCTGCGCCCC TCGGCCGCGA ACGGCCTCAC CCCAAAAATG GCAGCGCTGG CAGTCCTTGC CATTGCCGGG ATCGGGGCAG TAACGGGATG GGCGATCAGC CCGAGCGCGA CGCCCGGAAG CATTGACGTG CCGCAGGTGC TGGCATCGAC ATTCAGCGAC CAGGTGCCGG GCAGTGAGGG CGGCGGCCTG GGTGGCGGCC TGCCCTTCAC TTCGGCCGTC GGGGCATTCA CGGACTTCAT GGCGGGGCCG GCAATTTTTA CCTTGGGCAT TCTTGGCATA GTGGTCGCGG GTGCCGTGCT CGTGTTCGGG GGTGCGATAA ACCCAGCGAA CCATTTGAGG TGATAGGTAA GATTATACCG AGGTATGAAA ACGAGAATTG GACCTTTACA GAATTACTCT ATGAAGCGCC ATATTTAAAA AGCTACCAAG ACGAAGAGGA TGAAGAGGAT GAGGAGGCAG ATTGCCTTGA ATATATTGAC AATACTGATA AGATAATATA TCTTTTATAT AGAAGATATC GCCGTATGTA AGGATTTCAG GGGGCAAGGC ATAGGCAGCG CGCTTATCAA TATATCTATA GAATGGGCAA AGCATAAAAA CTTGCATGGA CTAATGCTTG AAACCCAGGA CAATAACCTT ATAGCTTGTA AATTCTATCA TAATTGGGTA ATGACTCCAA CTTATTGATA GTGTTTTATG TTCAGATAAT GCCCGATGAC TTTGTCATGC AGCTCCACCG ATTTTGAGAA CGACAGCGAC TTCCGTCCCA GCCGTGCCAG GTGCTGCCTC AGATTCAGGT TATGCCGCTC AATTCGCTGC GTATATCGCT TGCTGATTAC GTGCAGCTTT CCCTTCAGGC GGGATTCATA CAGCGGCCAG CCATCCGTCA TCCATATCAC CACGTCAAAG GGTGACAGCA GGCTCATAAG ACGCCCCAGC GTCGCCATAG TGCGTTCACC GAATACGTGC GCAACAACCG TCTTCCGGAG ACTGTCATAC GCGTAAAACA GCCAGCGCTG GCGCGATTTA GCCCCGACAT AGCCCCACTG TTCGTCCATT TCCGCGCAGA CGATGACGTC ACTGCCCGGC TGTATGCGCG AGGTTACCGA CTGCGGCCTG AGTTTTTTAA GTGACGTAAA ATCGTGTTGA GGCCAACGCC CATAATGCGG GCTGTTGCCC GGCATCCAAC GCCATTCATG GCCATATCAA TGATTTTCTG GTGCGTACCG GGTTGAGAAG CGGTGTAAGT GAACTGCAGT TGCCATGTTT TACGGCAGTG AGAGCAGAGA TAGCGCTGAT GTCCGGCGGT GCTTTTGCCG TTACGCACCA CCCCGTCAGT AGCTGAACAG GAGGGACAGC TGATAGACAC AGAAGCCACT GGAGCACCTC AAAAACACCA TCATACACTA AATCAGTAAG TTGGCAGCAT CACCCATAAT TGTGGTTTCA AAATCGGCTC CGTCGATACT ATGTTATACG CCAACTTTGA AAACAACTTT GAAAAAGCTG TTTTCTGGTA TTTAAGGTTT TAGAATGCAA GGAACAGTGA ATTGGAGTTC GTCTTGTTAT AATTAGCTTC TTGGGGTATC TTTAAATACT GTAGAAAAGA GGAAGGAAAT AATAAATGGC TAAAATGAGA ATATCACCGG AATTGAAAAA ACTGATCGAA AAATACCGCT GCGTAAAAGA TACGGAAGGA ATGTCTCCTG CTAAGGTATA TAAGCTGGTG GGAGAAAATG AAAACCTATA TTTAAAAATG ACGGACAGCC GGTATAAAGG GACCACCTAT GATGTGGAAC GGGAAAAGGA CATGATGCTA TGGCTGGAAG GAAAGCTGCC TGTTCCAAAG GTCCTGCACT TTGAACGGCA TGATGGCTGG AGCAATCTGC TCATGAGTGA GGCCGATGGC GTCCTTTGCT CGGAAGAGTA TGAAGATGAA CAAAGCCCTG AAAAGATTAT CGAGCTGTAT GCGGAGTGCA TCAGGCTCTT TCACTCCATC GACATATCGG ATTGTCCCTA TACGAATAGC TTAGACAGCC GCTTAGCCGA ATTGGATTAC TTACTGAATA ACGATCTGGC CGATGTGGAT TGCGAAAACT GGGAAGAAGA CACTCCATTT AAAGATCCGC GCGAGCTGTA TGATTTTTTA AAGACGGAAA AGCCCGAAGA GGAACTTGTC TTTTCCCACG GCGACCTGGG AGACAGCAAC ATCTTTGTGA AAGATGGCAA AGTAAGTGGC TTTATTGATC TTGGGAGAAG CGGCAGGGCG GACAAGTGGT ATGACATTGC CTTCTGCGTC CGGTCGATCA GGGAGGATAT CGGGGAAGAA CAGTATGTCG AGCTATTTTT TGACTTACTG GGGATCAAGC CTGATTGGGA GAAAATAAAA TATTATATTT TACTGGATGA ATTGTTTTAG TACCTAGATG TGGCGCAACG ATGCCGGCGA CAAGCAGGAG CGCACCGACT TCTTCCGCAT CAAGTGTTTT GGCTCTCAGG CCGAGGCCCA CGGCAAGTAT TTGGGCAAGG GGTCGCTGGT ATTCGTGCAG GGCAAGATTC GGAATACCAA GTACGAGAAG GACGGCCAGA CGGTCTACGG GACCGACTTC ATTGCCGATA AGGTGGATTA TCTGGACACC AAGGCACCAG GCGGGTCAAA TCAGGAATAA GGGCACATTG CCCCGGCGTG AGTCGGGGCA ATCCCGCAAG GAGGGTGAAT GAATCGGACG TTTGACCGGA AGGCATACAG GCAAGAACTG ATCGACGCGG GGTTTTCCGC CGAGGATGCC GAAACCATCG CAAGCCGCAC CGTCATGCGT GCGCCCCGCG AAACCTTCCA GTCCGTCGGC TCGATGGTCC AGCAAGCTAC GGCCAAGATC GAGCGCGACA GCGTGCAACT GGCTCCCCCT GCCCTGCCCG CGCCATCGGC CGCCGTGGAG CGTTCGCGTC GTCTCGAACA GGAGGCGGCA GGTTTGGCGA AGTCGATGAC CATCGACACG CGAGGAACTA TGACGACCAA GAAGCGAAAA ACCGCCGGCG AGGACCTGGC AAAACAGGTC AGCGAGGCCA AGCAGGCCGC GTTGCTGAAA CACACGAAGC AGCAGATCAA GGAAATGCAG CTTTCCTTGT TCGATATTGC GCCGTGGCCG GACACGATGC GAGCGATGCC AAACGACACG GCCCGCTCTG CCCTGTTCAC CACGCGCAAC AAGAAAATCC CGCGCGAGGC GCTGCAAAAC AAGGTCATTT TCCACGTCAA CAAGGACGTG AAGATCACCT ACACCGGCGT CGAGCTGCGG GCCGACGATG ACGAACTGGT GTGGCAGCAG GTGTTGGAGT ACGCGAAGCG CACCCCTATC GGCGAGCCGA TCACCTTCAC GTTCTACGAG CTTTGCCAGG ACCTGGGCTG GTCGATCAAT GGCCGGTATT ACACGAAGGC CGAGGAATGC CTGTCGCGCC TACAGGCGAC GGCGATGGGC TTCACGTCCG ACCGCGTTGG GCACCTGGAA TCGGTGTCGC TGCTGCACCG CTTCCGCGTC CTGGACCGTG GCAAGAAAAC GTCCCGTTGC CAGGTCCTGA TCGACGAGGA AATCGTCGTG CTGTTTGCTG GCGACCACTA CACGAAATTC ATATGGGAGA AGTACCGCAA GCTGTCGCCG ACGGCCCGAC GGATGTTCGA CTATTTCAGC TCGCACCGGG AGCCGTACCC GCTCAAGCTG GAAACCTTCC GCCTCATGTG CGGATCGGAT TCCACCCGCG TGAAGAAGTG GCGCGAGCAG GTCGGCGAAG CCTGCGAAGA GTTGCGAGGC AGCGGCCTGG TGGAACACGC CTGGGTCAAT GATGACCTGG TGCATTGCAA ACGCTAGGGC CTTGTGGGGT CAGTTCCGGC TGGGGGTTCA GCAGCCAGCG CTTTACTGGC ATTTCAGGAA CAAGCGGGCA CTGCTCGACG CACTTGCTTC GCTCAGTATC GCTCGGGACG CACGGCGCGC TCTACGAACT GCCGATAAAC AGAGGATTAA AATTGACAAT TGTGATTAAG GCTCAGATTC GACGGCTTGG AGCGGCCGAC GTGCAGGATT TCCGCGAGAT CCGATTGTCG GCCCTGAAGA AAGCTCCAGA GATGTTCGGG TCCGTTTACG AGCACGAGGA GAAAAAGCCC ATGGAGGCGT TCGCTGAACG GTTGCGAGAT GCCGTGGCAT TCGGCGCCTA CATCGACGGC GAGATCATTG GGCTGTCGGT CTTCAAACAG GAGGACGGCC CCAAGGACGC TCACAAGGCG CATCTGTCCG GCGTTTTCGT GGAGCCCGAA CAGCGAGGCC GAGGGGTCGC CGGTATGCTG CTGCGGGCGT TGCCGGCGGG TTTATTGCTC GTGATGATCG TCCGACAGAT TCCAACGGGA ATCTGGTGGA TGCGCATCTT CATCCTCGGC GCACTTAATA TTTCGCTATT CTGGAGCTTG TTGTTTATTT CGGTCTACCG CCTGCCGGGC GGGGTCGCGG CGACGGTAGG CGCTGTGCAG CCGCTGATGG TCGTGTTCAT CTCTGCCGCT CTGCTAGGTA GCCCGATACG ATTGATGGCG GTCCTGGGGG CTATTTGCGG AACTGCGGGC GTGGCGCTGT TGGTGTTGAC ACCAAACGCA GCGCTAGATC CTGTCGGCGT CGCAGCGGGC CTGGCGGGGG CGGTTTCCAT GGCGTTCGGA ACCGTGCTGA CCCGCAAGTG GCAACCTCCC GTGCCTCTGC TCACCTTTAC CGCCTGGCAA CTGGCGGCCG GAGGACTTCT GCTCGTTCCA GTAGCTTTAG TGTTTGATCC GCCAATCCCG ATGCCTACAG GAACCAATGT TCTCGGCCTG GCGTGGCTCG GCCTGATCGG AGCGGGTTTA ACCTACTTCC TTTGGTTCCG GGGGATCTCG CGACTCGAAC CTACAGTTGT TTCCTTACTG GGCTTTCTCA GCCCCAGATC TGGGGTCGAT CAGCCGGGGA TGCATCAGGC CGACAGTCGG AACTTCGGGT CCCCGACCTG TACCATTCGG TGAGCAATGG ATAGGGGAGT TGATATCGTC AACGTTCACT TCTAAAGAAA TAGCGCCACT CAGCTTCCTC AGCGGCTTTA TCCAGCGATT TCCTATTATG TCGGCATAGT TCTCAAGATC GACAGCCTGT CACGGTTAAG CGAGAAATGA ATAAGAAGGC TGATAATTCG GATCTCTGCG AGGGAGATGA TATTTGATCA CAGGCAGCAA CGCTCTGTCA TCGTTACAAT CAACATGCTA CCCTCCGCGA GATCATCCGT GTTTCAAACC CGGCAGCTTA GTTGCCGTTC TTCCGAATAG CATCGGTAAC ATGAGCAAAG TCTGCCGCCT TACAACGGCT CTCCCGCTGA CGCCGTCCCG GACTGATGGG CTGCCTGTAT CGAGTGGTGA TTTTGTGCCG AGCTGCCGGT CGGGGAGCTG TTGGCTGGCT GGTGGCAGGA TATATTGTGG TGTAAACAAA TTGACGCTTA GACAACTTAA TAACACATTG CGGACGTTTT TAATGTACTG GGGTGGTTTT TCTTTTCACC AGTGAGACGG GCAACAGCTG ATTGCCCTTC ACCGCCTGGC CCTGAGAGAG TTGCAGCAAG CGGTCCACGC TGGTTTGCCC CAGCAGGCGA AAATCCTGTT TGATGGTGGT TCCGAAATCG GCAAAATCCC TTATAAATCA AAAGAATAGC CCGAGATAGG GTTGAGTGTT GTTCCAGTTT GGAACAAGAG TCCACTATTA AAGAACGTGG ACTCCAACGT CAAAGGGCGA AAAACCGTCT ATCAGGGCGA TGGCCCACTA CGTGAACCAT CACCCAAATC AAGTTTTTTG GGGTCGAGGT GCCGTAAAGC ACTAAATCGG AACCCTAAAG GGAGCCCCCG ATTTAGAGCT TGACGGGGAA AGCCGGCGAA CGTGGCGAGA AAGGAAGGGA AGAAAGCGAA AGGAGCGGGC GCCATTCAGG CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG CCAGCTGGCG AAAGGGGGAT GTGCTGCAAG GCGATTAAGT TGGGTAACGC CAGGGTTTTC CCAGTCACGA CGTTGTAAAA CGACGGCCAG TGAATTCGAG CTCGGTACCC GGGGATCCTC TAGAGTCGAC CTGCAGGCAT GCAAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCCA AAGACAAAAG GGCGACATTC AACCGATTGA GGGAGGGAAG GTAAATATTG ACGGAAATTA TTCATTAAAG GTGAATTATC ACCGTCACCG ACTTGAGCCA TTTGGGAATT AGAGCCAGCA AAATCACCAG TAGCACCATT ACCATTAGCA AGGCCGGAAA CGTCACCAAT GAAACCATCG ATAGCAGCAC CGTAATCAGT AGCGACAGAA TCAAGTTTGC CTTTAGCGTC AGACTGTAGC GCGTTTTCAT CGGCATTTTC GGTCATAGCC CCCTTATTAG CGTTTGCCAT CTTTTCATAA TCAAAATCAC CGGAACCAGA GCCACCACCG GAACCGCCTC CCTCAGAGCC GCCACCCTCA GAACCGCCAC CCTCAGAGCC ACCACCCTCA GAGCCGCCAC CAGAACCACC ACCAGAGCCG CCGCCAGCAT TGACAGGAGG CCCGATCTAG TAACATAGAT GACACCGCGC GCGATAATTT ATCCTAGTTT GCGCGCTATA TTTTGTTTTC TATCGCGTAT TAAATGTATA ATTGCGGGAC TCTAATCATA AAAACCCATC TCATAAATAA CGTCATGCAT TACATGTTAA TTATTACATG CTTAACGTAA TTCAACAGAA ATTATATGAT AATCATCGCA AGACCGGCAA CAGGATTCAA TCTTAAGAAA CTTTATTGCC AAATGTTTGA ACGATCGGGG ATCATCCGGG TCTGTGGCGG GAACTCCACG AAAATATCCG AACGCAGCAA GATATCGCGG TGCATCTCGG TCTTGCCTGG GCAGTCGCCG CCGACGCCGT TGATGTGGAC GCCGGGCCCG ATCATATTGT CGCTCAGGAT CGTGGCGTTG TGCTTGTCGG CCGTTGCTGT CGTAATGATA TCGGCACCTT CGACCGCCTG TTCCGCAGAG ATCCCGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG CGGTCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCATCGCC GTCGGGCATG CGCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCCTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT CCAGATCCGG TGCAGATTAT TTGGATTGAG AGTGAATATG AGACTCTAAT TGGATACCGA GGGGAATTTA TGGAACGTCA GTGGAGCATT TTTGACAAGA AATATTTGCT AGCTGATAGT GACCTTAGGC GACTTTTGAA CGCGCAATAA TGGTTTCTGA CGTATGTGCT TAGCTCATTA AACTCCAGAA ACCCGCGGCT GAGTGGCTCC TTCAACGTTG CGGTTCTGTC AGTTCCAAAC GTAAAACGGC TTGTCCCGCG TCATCGGCGG GGGTCATAAC GTGACTCCCT TAATTCTCCG CTCATGATCA GATTGTCGTT TCCCGCCTTC AGTTTAAACT ATCAGTGTTT GACAGGATAT ATTGGCGGGT AAACCTAAGA GAAAAGAGCG TTTATTAGAA TAATCGGATA TTTAAAAGGG CGTGAAAAGG TTTATCCGTT CGTCCATTTG TATGTGCATG CCAACCACAG GGTTCCCCAG ATCTGGCGCC GGCCAGCGAG ACGAGCAAGA TTGGCCGCCG CCCGAAACGA TCCGACAGCG CGCCCAGCAC AGGTGCGCAG GCAAATTGCA CCAACGCATA CAGCGCCAGC AGAATGCCAT AGTGGGCGGT GACGTCGTTC GAGTGAACCA GATCGCGCAG GAGGCCCGGC AGCACCGGCA TAATCAGGCC GATGCCGACA GCGTCGAGCG CGACAGTGCT CAGAATTACG ATCAGGGGTA TGTTGGGTTT CACGTCTGGC CTCCGGACCA GCCTCCGCTG GTCCGATTGA ACGCGCGGAT TCTTTATCAC TGATAAGTTG GTGGACATAT TATGTTTATC AGTGATAAAG TGTCAAGCAT GACAAAGTTG CAGCCGAATA CAGTGATCCG TGCCGCCCTG GACCTGTTGA ACGAGGTCGG CGTAGACGGT CTGACGACAC GCAAACTGGC GGAACGGTTG GGGGTTCAGC AGCCGGCGCT TTACTGGCAC TTCAGGAACA AGCGGGCGCT GCTCGACGCA CTGGCCGAAG CCATGCTGGC GGAGAATCAT ACGCATTCGG TGCCGAGAGC CGACGACGAC TGGCGCTCAT TTCTGATCGG GAATGCCCGC AGCTTCAGGC AGGCGCTGCT CGCCTACCGC GATGGCGCGC GCATCCATGC CGGCACGCGA CCGGGCGCAC CGCAGATGGA AACGGCCGAC GCGCAGCTTC GCTTCCTCTG CGAGGCGGGT TTTTCGGCCG GGGACGCCGT CAATGCGCTG ATGACAATCA GCTACTTCAC TGTTGGGGCC GTGCTTGAGG AGCAGGCCGG CGACAGCGAT GCCGGCGAGC GCGGCGGCAC CGTTGAACAG GCTCCGCTCT CGCCGCTGTT GCGGGCCGCG ATAGACGCCT TCGACGAAGC CGGTCCGGAC GCAGCGTTCG AGCAGGGACT CGCGGTGATT GTCGATGGAT TGGCGAAAAG GAGGCTCGTT GTCAGGAACG TTGAAGGACC GAGAAAGGGT GACGATTGAT CAGGACCGCT GCCGGAGCGC AACCCACTCA CTACAGCAGA GCCATGTAGA CAACATCCCC TCCCCCTTTC CACCGCGTCA GACGCCCGTA GCAGCCCGCT ACGGGCTTTT TCATGCCCTG CCCTAGCGTC CAAGCCTCAC GGCCGCGCTC GGCCTCTCTG GCGGCCTTCT GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTTC CGCTGCATAA CCCTGCTTCG GGGTCATTAT AGCGATTTTT TCGGTATATC CATCCTTTTT CGCACGATAT ACAGGATTTT GCCAAAGGGT TCGTGTAGAC TTTCCTTGGT GTATCCAACG GCGTCAGCCG GGCAGGATAG GTGAAGTAGG CCCACCCGCG AGCGGGTGTT CCTTCTTCAC TGTCCCTTAT TCGCACCTGG CGGTGCTCAA CGGGAATCCT GCTCTGCGAG GCTGGCCGGC TACCGCCGGC GTAACAGATG AGGGCAAGCG GATGGCTGAT GAAACCAAGC CAACCAGGAA GGGCAGCCCA CCTATCAAGG TGTACTGCCT TCCAGACGAA CGAAGAGCGA TTGAGGAAAA GGCGGCGGCG GCCGGCATGA GCCTGTCGGC CTACCTGCTG GCCGTCGGCC AGGGCTACAA AATCACGGGC GTCGTGGACT ATGAGCACGT CCGCGAGCTG GCCCGCATCA ATGGCGACCT GGGCCGCCTG GGCGGCCTGC TGAAACTCTG GCTCACCGAC GACCCGCGCA CGGCGCGGTT CGGTGATGCC ACGATCCTCG CCCTGCTGGC GAAGATCGAA GAGAAGCAGG ACGAGCTTGG CAAGGTCATG ATGGGCGTGG TCCGCCCGAG GGCAGAGCCA TGACTTTTTT AGCCGCTAAA ACGGCCGGGG GGTGCGCGTG ATTGCCAAGC ACGTCCCCAT GCGCTCCATC AAGAAGAGCG ACTTCGCGGA GCTGGTGAAG TACATCACCG ACGAGCAAGG CAAGACCGAG CGCCTTTGCG ACGCTCA
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