pCAMBIA0390
$128.00
In stock
SKU
PL83920
100μg liquid plasmid(Instantly):$163
| name | pCAMBIA0390 | another name | apCAMBIA0390 |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pVS1 | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | low | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CTAGCCACCA CCACCACCAC CACGTGTGAA TTACAGGTGA CCAGCTCGAA TTTCCCCGAT CGTTCAAACA TTTGGCAATA AAGTTTCTTA AGATTGAATC CTGTTGCCGG TCTTGCGATG ATTATCATAT AATTTCTGTT GAATTACGTT AAGCATGTAA TAATTAACAT GTAATGCATG ACGTTATTTA TGAGATGGGT TTTTATGATT AGAGTCCCGC AATTATACAT TTAATACGCG ATAGAAAACA AAATATAGCG CGCAAACTAG GATAAATTAT CGCGCGCGGT GTCATCTATG TTACTAGATC GGGAATTAAA CTATCAGTGT TTGACAGGAT ATATTGGCGG GTAAACCTAA GAGAAAAGAG CGTTTATTAG AATAACGGAT ATTTAAAAGG GCGTGAAAAG GTTTATCCGT TCGTCCATTT GTATGTGCAT GCCAACCACA GGGTTCCCCT CGGGATCAAA GTACTTTGAT CCAACCCCTC CGCTGCTATA GTGCAGTCGG CTTCTGACGT TCAGTGCAGC CGTCTTCTGA AAACGACATG TCGCACAAGT CCTAAGTTAC GCGACAGGCT GCCGCCCTGC CCTTTTCCTG GCGTTTTCTT GTCGCGTGTT TTAGTCGCAT AAAGTAGAAT ACTTGCGACT AGAACCGGAG ACATTACGCC ATGAACAAGA GCGCCGCCGC TGGCCTGCTG GGCTATGCCC GCGTCAGCAC CGACGACCAG GACTTGACCA ACCAACGGGC CGAACTGCAC GCGGCCGGCT GCACCAAGCT GTTTTCCGAG AAGATCACCG GCACCAGGCG CGACCGCCCG GAGCTGGCCA GGATGCTTGA CCACCTACGC CCTGGCGACG TTGTGACAGT GACCAGGCTA GACCGCCTGG CCCGCAGCAC CCGCGACCTA CTGGACATTG CCGAGCGCAT CCAGGAGGCC GGCGCGGGCC TGCGTAGCCT GGCAGAGCCG TGGGCCGACA CCACCACGCC GGCCGGCCGC ATGGTGTTGA CCGTGTTCGC CGGCATTGCC GAGTTCGAGC GTTCCCTAAT CATCGACCGC ACCCGGAGCG GGCGCGAGGC CGCCAAGGCC CGAGGCGTGA AGTTTGGCCC CCGCCCTACC CTCACCCCGG CACAGATCGC GCACGCCCGC GAGCTGATCG ACCAGGAAGG CCGCACCGTG AAAGAGGCGG CTGCACTGCT TGGCGTGCAT CGCTCGACCC TGTACCGCGC ACTTGAGCGC AGCGAGGAAG TGACGCCCAC CGAGGCCAGG CGGCGCGGTG CCTTCCGTGA GGACGCATTG ACCGAGGCCG ACGCCCTGGC GGCCGCCGAG AATGAACGCC AAGAGGAACA AGCATGAAAC CGCACCAGGA CGGCCAGGAC GAACCGTTTT TCATTACCGA AGAGATCGAG GCGGAGATGA TCGCGGCCGG GTACGTGTTC GAGCCGCCCG CGCACGTCTC AACCGTGCGG CTGCATGAAA TCCTGGCCGG TTTGTCTGAT GCCAAGCTGG CGGCCTGGCC GGCCAGCTTG GCCGCTGAAG AAACCGAGCG CCGCCGTCTA AAAAGGTGAT GTGTATTTGA GTAAAACAGC TTGCGTCATG CGGTCGCTGC GTATATGATG CGATGAGTAA ATAAACAAAT ACGCAAGGGG AACGCATGAA GGTTATCGCT GTACTTAACC AGAAAGGCGG GTCAGGCAAG ACGACCATCG CAACCCATCT AGCCCGCGCC CTGCAACTCG CCGGGGCCGA TGTTCTGTTA GTCGATTCCG ATCCCCAGGG CAGTGCCCGC GATTGGGCGG CCGTGCGGGA AGATCAACCG CTAACCGTTG TCGGCATCGA CCGCCCGACG ATTGACCGCG ACGTGAAGGC CATCGGCCGG CGCGACTTCG TAGTGATCGA CGGAGCGCCC CAGGCGGCGG ACTTGGCTGT GTCCGCGATC AAGGCAGCCG ACTTCGTGCT GATTCCGGTG CAGCCAAGCC CTTACGACAT ATGGGCCACC GCCGACCTGG TGGAGCTGGT TAAGCAGCGC ATTGAGGTCA CGGATGGAAG GCTACAAGCG GCCTTTGTCG TGTCGCGGGC GATCAAAGGC ACGCGCATCG GCGGTGAGGT TGCCGAGGCG CTGGCCGGGT ACGAGCTGCC CATTCTTGAG TCCCGTATCA CGCAGCGCGT GAGCTACCCA GGCACTGCCG CCGCCGGCAC AACCGTTCTT GAATCAGAAC CCGAGGGCGA CGCTGCCCGC GAGGTCCAGG CGCTGGCCGC TGAAATTAAA TCAAAACTCA TTTGAGTTAA TGAGGTAAAG AGAAAATGAG CAAAAGCACA AACACGCTAA GTGCCGGCCG TCCGAGCGCA CGCAGCAGCA AGGCTGCAAC GTTGGCCAGC CTGGCAGACA CGCCAGCCAT GAAGCGGGTC AACTTTCAGT TGCCGGCGGA GGATCACACC AAGCTGAAGA TGTACGCGGT ACGCCAAGGC AAGACCATTA CCGAGCTGCT ATCTGAATAC ATCGCGCAGC TACCAGAGTA AATGAGCAAA TGAATAAATG AGTAGATGAA TTTTAGCGGC TAAAGGAGGC GGCATGGAAA ATCAAGAACA ACCAGGCACC GACGCCGTGG AATGCCCCAT GTGTGGAGGA ACGGGCGGTT GGCCAGGCGT AAGCGGCTGG GTTGTCTGCC GGCCCTGCAA TGGCACTGGA ACCCCCAAGC CCGAGGAATC GGCGTGAGCG GTCGCAAACC ATCCGGCCCG GTACAAATCG GCGCGGCGCT GGGTGATGAC CTGGTGGAGA AGTTGAAGGC CGCGCAGGCC GCCCAGCGGC AACGCATCGA GGCAGAAGCA CGCCCCGGTG AATCGTGGCA AGCGGCCGCT GATCGAATCC GCAAAGAATC CCGGCAACCG CCGGCAGCCG GTGCGCCGTC GATTAGGAAG CCGCCCAAGG GCGACGAGCA ACCAGATTTT TTCGTTCCGA TGCTCTATGA CGTGGGCACC CGCGATAGTC GCAGCATCAT GGACGTGGCC GTTTTCCGTC TGTCGAAGCG TGACCGACGA GCTGGCGAGG TGATCCGCTA CGAGCTTCCA GACGGGCACG TAGAGGTTTC CGCAGGGCCG GCCGGCATGG CCAGTGTGTG GGATTACGAC CTGGTACTGA TGGCGGTTTC CCATCTAACC GAATCCATGA ACCGATACCG GGAAGGGAAG GGAGACAAGC CCGGCCGCGT GTTCCGTCCA CACGTTGCGG ACGTACTCAA GTTCTGCCGG CGAGCCGATG GCGGAAAGCA GAAAGACGAC CTGGTAGAAA CCTGCATTCG GTTAAACACC ACGCACGTTG CCATGCAGCG TACGAAGAAG GCCAAGAACG GCCGCCTGGT GACGGTATCC GAGGGTGAAG CCTTGATTAG CCGCTACAAG ATCGTAAAGA GCGAAACCGG GCGGCCGGAG TACATCGAGA TCGAGCTAGC TGATTGGATG TACCGCGAGA TCACAGAAGG CAAGAACCCG GACGTGCTGA CGGTTCACCC CGATTACTTT TTGATCGATC CCGGCATCGG CCGTTTTCTC TACCGCCTGG CACGCCGCGC CGCAGGCAAG GCAGAAGCCA GATGGTTGTT CAAGACGATC TACGAACGCA GTGGCAGCGC CGGAGAGTTC AAGAAGTTCT GTTTCACCGT GCGCAAGCTG ATCGGGTCAA ATGACCTGCC GGAGTACGAT TTGAAGGAGG AGGCGGGGCA GGCTGGCCCG ATCCTAGTCA TGCGCTACCG CAACCTGATC GAGGGCGAAG CATCCGCCGG TTCCTAATGT ACGGAGCAGA TGCTAGGGCA AATTGCCCTA GCAGGGGAAA AAGGTCGAAA AGGTCTCTTT CCTGTGGATA GCACGTACAT TGGGAACCCA AAGCCGTACA TTGGGAACCG GAACCCGTAC ATTGGGAACC CAAAGCCGTA CATTGGGAAC CGGTCACACA TGTAAGTGAC TGATATAAAA GAGAAAAAAG GCGATTTTTC CGCCTAAAAC TCTTTAAAAC TTATTAAAAC TCTTAAAACC CGCCTGGCCT GTGCATAACT GTCTGGCCAG CGCACAGCCG AAGAGCTGCA AAAAGCGCCT ACCCTTCGGT CGCTGCGCTC CCTACGCCCC GCCGCTTCGC GTCGGCCTAT CGCGGCCGCT GGCCGCTCAA AAATGGCTGG CCTACGGCCA GGCAATCTAC CAGGGCGCGG ACAAGCCGCG CCGTCGCCAC TCGACCGCCG GCGCCCACAT CAAGGCACCC TGCCTCGCGC GTTTCGGTGA TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGAGACG GTCACAGCTT GTCTGTAAGC GGATGCCGGG AGCAGACAAG CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG CGCAGCCATG ACCCAGTCAC GTAGCGATAG CGGAGTGTAT ACTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCAT ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG CATTCTAGGT ACTAAAACAA TTCATCCAGT AAAATATAAT ATTTTATTTT CTCCCAATCA GGCTTGATCC CCAGTAAGTC AAAAAATAGC TCGACATACT GTTCTTCCCC GATATCCTCC CTGATCGACC GGACGCAGAA GGCAATGTCA TACCACTTGT CCGCCCTGCC GCTTCTCCCA AGATCAATAA AGCCACTTAC TTTGCCATCT TTCACAAAGA TGTTGCTGTC TCCCAGGTCG CCGTGGGAAA AGACAAGTTC CTCTTCGGGC TTTTCCGTCT TTAAAAAATC ATACAGCTCG CGCGGATCTT TAAATGGAGT GTCTTCTTCC CAGTTTTCGC AATCCACATC GGCCAGATCG TTATTCAGTA AGTAATCCAA TTCGGCTAAG CGGCTGTCTA AGCTATTCGT ATAGGGACAA TCCGATATGT CGATGGAGTG AAAGAGCCTG ATGCACTCCG CATACAGCTC GATAATCTTT TCAGGGCTTT GTTCATCTTC ATACTCTTCC GAGCAAAGGA CGCCATCGGC CTCACTCATG AGCAGATTGC TCCAGCCATC ATGCCGTTCA AAGTGCAGGA CCTTTGGAAC AGGCAGCTTT CCTTCCAGCC ATAGCATCAT GTCCTTTTCC CGTTCCACAT CATAGGTGGT CCCTTTATAC CGGCTGTCCG TCATTTTTAA ATATAGGTTT TCATTTTCTC CCACCAGCTT ATATACCTTA GCAGGAGACA TTCCTTCCGT ATCTTTTACG CAGCGGTATT TTTCGATCAG TTTTTTCAAT TCCGGTGATA TTCTCATTTT AGCCATTTAT TATTTCCTTC CTCTTTTCTA CAGTATTTAA AGATACCCCA AGAAGCTAAT TATAACAAGA CGAACTCCAA TTCACTGTTC CTTGCATTCT AAAACCTTAA ATACCAGAAA ACAGCTTTTT CAAAGTTGTT TTCAAAGTTG GCGTATAACA TAGTATCGAC GGAGCCGATT TTGAAACCGC GGTGATCACA GGCAGCAACG CTCTGTCATC GTTACAATCA ACATGCTACC CTCCGCGAGA TCATCCGTGT TTCAAACCCG GCAGCTTAGT TGCCGTTCTT CCGAATAGCA TCGGTAACAT GAGCAAAGTC TGCCGCCTTA CAACGGCTCT CCCGCTGACG CCGTCCCGGA CTGATGGGCT GCCTGTATCG AGTGGTGATT TTGTGCCGAG CTGCCGGTCG GGGAGCTGTT GGCTGGCTGG TGGCAGGATA TATTGTGGTG TAAACAAATT GACGCTTAGA CAACTTAATA ACACATTGCG GACGTTTTTA ATGTACTGAA TTAACGCCGA ATTAATTCCT AGGCCACCAT GTTGGGCCCG GCGCGCCAAG CTTGGCTGCA GGTCGACGGA TCCCCGGGAA TTCTAAGAGG AGTCCACCAT GGTAGATCTG ACTAGTGTTA ACG
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