pHY300PLK
$128.00
In stock
SKU
PL83913
100μg liquid plasmid(Instantly):$163
| name | pHY300PLK | another name | apHY300PLK |
| Insert Size (bp) | Plasmid host | Bacillus subtilis | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | p15A | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | low | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AAGCTTCTAG AGATCTGCAG GTCGACGGAT CCCCGGGAAT TCCTGTTATA AAAAAAGGAT CAATTTTGAA CTCTCTCCCA AAGTTGATCC CTTAACGATT TAGAAATCCC TTTGAGAATG TTTATATACA TTCAAGGTAA CCAGCCAACT AATGACAATG ATTCCTGAAA AAAGTAATAA CAAATTACTA TACAGATAAG TTGACTGATC AACTTCCATA GGTAACAACC TTTGATCAAG TAAGGGTATG GATAATAAAC CACCTACAAT TGCAATACCT GTTCCCTCTG ATAAAAAGCT GGTAAAGTTA AGCAAACTCA TTCCAGCACC AGCTTCCTGC TGTTTCAAGC TACTTGAAAC AATTGTTGAT ATAACTGTTT TGGTGAACGA AAGCCCACCT AAAACAAATA CGATTATAAT TGTCATGAAC CATGATGTTG TTTCTAAAAG AAAGGAAGCA GTTAAAAAGC TAACAGAAAG AAATGTAACT CCGATGTTTA ACACGTATAA AGGACCTCTT CTATCAACAA GTATCCCACC AATGTAGCCG AAAATAATGA CACTCATTGT TCCAGGGAAA ATAATTACAC TTCCGATTTC GGCAGTACTT AGCTGGTGAA CATCTTTCAT CATATAAGGA ACCATAGAGA CAAACCCTGC TACTGTTCCA AATATAATTC CCCCACAAAG AACTCCAATC ATAAAAGGTA TATTTTTCCC TAATCCGGGA TCAACAAAAG GATCTGTTAC TTTCCTGATA TGTTTTACAA ATATCAGGAA TGACAGCACG CTAACGATAA GAAAAGAAAT GCTATATGAT GTTGTAAACA ACATAAAAAA TACAATGCCT ACAGACATTA GTATAATTCC TTTGATATCA AAATGACCTT TTATCCTTAC TTCTTTCTTT AATAATTTCA TAAGAAACGG AACAGTGATA ATTGTTATCA TAGGAATGAG TAGAAGATAG GACCAATGAA TATAATGGGC TATCATTCCA CCAATCGCTG GACCGACTCC TTCTCCCATG GCTACTATCG ATCCAATAAG ACCAAATGCT TTACCCCTAT TTTCCTTTGG AATATAGCGC GCAACTACAA CCATTACGAG TGCTGGAAAT GCAGCTGCAC CAGCCCCTTG AATAAAACGA GCCATAATAA GTAAGGAAAA GAAAGAATGG CCAACAAACC CAATTACCGA CCCGAAACAA TTTATTATAA TTCCAAATAG GAGTAACCTT TTGATGCCTA ATTGATCAGA TAGCTTTCCA TATACAGCTG TTCCAATGGA AAAGGTTAAC ATAAAGGCTG TGTTCACCCA GTTTGTACTC GCAGGTGGTT TATTAAAATC ATTTGCAATA TCAGGTAATG AGACGTTCAA AACCATTTCA TTTAATACGC TAAAAAAAGA TAAAATGCAA AGCCAAATTA AAATTTGGTT GTGTCGTAAA TTCGATTGTG AATAGGATGT ATTCACATTT CACCCTCCAA TAATGAGGGC AGACGTAGTT TATAGGGTTA ATGATACGCT TCCCTCTTTT AATTGAACCC TGTTACATTC ATTATTCATT ACACTTCATA ATTAATTCCT CCTAAACTTG ATTAAAACAT TTTACCACAT ATAAACTAAG TTTTAAATTC AGTATTTCAT CACTTATACA ACAATATGGC CCGTTTGTTG AACTACTCTT TAATAAAATA ATTTTTCCGT TCCCAATTCC ACATTGCAAT AATAGAAAAT CCATCTTCAT CGGCTTTTTC GTCATCATCT GTATGAATCA AATCGCCTTC TTCTGTGTCA TCAAGGTTTA ATTTTTTATG TATTTCTTTT AACAAACCAC CATAGGAGAT TAACCTTTTA CGGTGTAAAC CTTCCTCCAA ATCAGACAAA CGTTTCAAAT TCTTTTCTTC ATCATCGGTC ATAAAATCCG TATCCTTTAC AGGATATTTT GCAGTTTCGT CAATTGCCGA TTGTATATCC GATTTATATT TATTTTTCGG TCGAATCATT TGAACTTTTA CATTTGGATC ATAGTCTAAT TTCATTGCCT TTTTCCAAAA TTGAATCCAT TGTTTTTGAT TCACGTAGTT TTCTGTATTC TTAAAATAAG TTGGTTCCAC ACATACCAAT ACATGCATGT GCTGATTATA AGAATTATCT TTATTATTTA TTGTCACTTC CGTTGCACGC ATAAAACCAA CAAGATTTTT ATTAATTTTT TTATATTGCA TCATTCGGCG AAATCCTTGA GCCATATCTG ACAAACTCTT ATTTAATTCT TCGCCATCAT AAACATTTTT AACTGTTAAT GTGAGAAACA ACCAACGAAC TGTTGGCTTT TGTTTAATAA CTTCAGCAAC AACCTTTTGT GACTGAATGC CATGTTTCAT TGCTCTCCTC CAGTTGCACA TTGGACAAAG CCTGGATTTA CAAAACCACA CTCGATACAA CTTTCTTTCG CCTGTTTCAC GATTTTGTTT ATACTCTAAT ATTTCAGCAC AATCTTTTAC TCTTTCAGCC TTTTTAAATT CAAGAATATG CAGAAGTTCA AAGTAATCAA CATTAGCGAT TTTCTTTTCT CTCCATGGTC TCACTTTTCC ACTTTTTGTC TTGTCCACTA AAACCCTTGA TTTTTCATCT GAATAAATGC TACTATTAGG ACACATAATA TTAAAAGAAA CCCCCATCTA TTTAGTTATT TGTTTGGTCA CTTATAACTT TAACAGATGG GGTTTTTCTG TGCAACCAAT TTTAAGGGTT TTCCAATACT TTAAAACACA TACATACCAA CACTTCAACG CACCTTTCAG CAACTAAAAT AAAAATGACG TTATTTCTAT ATGTATCAAG ATAAGAAAGA ACAAGTTCAA AACCATCAAA AAAAGACACC TTTTCAGGTG CTTTTTTTAT TTTATAAACT CATTCCCTGA TCTCGACTTC GTTCTTTTTT TACCTCTCGG TTATGAGTTA GTTCAAATTC GTTCTTTTTA GGTTCTAAAT CGTGTTTTTC TTGGAATTGT GCTGTTTTAT CCTTTACCTT GTCTACAAAC CCCTTAAAAA CGTTTTTAAA GGCTTTTAAG CGTCTGTACG TTCCTTAAGG AATTATTCCT TAGTGCTTTC TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTCCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCTTAA TAAGATGATC TTCTTGAGAT CGTTTTGGTC TGCGCGTAAT CTCTTGCTCT GAAAACGAAA AAACCGCCTT GCAGGGAGGT TTTTCGAAGG TTCTCTGAGC TACCAACTCT TTGAACCGAG GTAACTGGCT TGCAGGAGCG CAGTCACCAA AACTTGTCCT TTCAGTTTAG CCTTAACCGG CGCATGACTT CAAGACTAAC TCCTCTAAAT CAATTACCAG TGGCTGCTGC CAGTGGTGCT TTTGCATGTC TTTCCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GACTGAACGG GGGGTTCGTG CATACAGTCC AGCTTGGAGC GAACTGCCTA CCCGGAACTG AGTGTCAGGC GTGGAATGAG ACAAACGCGG CCATAACAGC GGAATGACAC CGGTAAACCG AAAGGCAGGA ACAGGAGAGC GCACGAGGGA GCCGCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCACTGATT TGAGCGTCAG ATTTCGTGAT GCTTGTCAGG GGGGCGGAGC CTATGGAAAA ACGGCTTTGC CC
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