pAAV-RC5
$99.00
In stock
SKU
PL83880
pAAV-5;pAAV-5
100μg liquid plasmid(Instantly):$120
| name | pAAV-RC5 | another name | apAAV-RC5 |
| Insert Size (bp) | Plasmid host | Mammary cell,Adeno-associated virus | |
| Plasmid uses | packaging aid | Fragment type | |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pUC | |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CACCTAAATT GTAAGCGTTA ATATTTTGTT AAAATTCGCG TTAAATTTTT GTTAAATCAG CTCATTTTTT AACCAATAGG CCGAAATCGG CAAAATCCCT TATAAATCAA AAGAATAGAC CGAGATAGGG TTGAGTGTTG TTCCAGTTTG GAACAAGAGT CCACTATTAA AGAACGTGGA CTCCAACGTC AAAGGGCGAA AAACCGTCTA TCAGGGCGAT GGCCCACTAC GTGAACCATC ACCCTAATCA AGTTTTTTGG GGTCGAGGTG CCGTAAAGCA CTAAATCGGA ACCCTAAAGG GAGCCCCCGA TTTAGAGCTT GACGGGGAAA GCCGGCGAAC GTGGCGAGAA AGGAAGGGAA GAAAGCGAAA GGAGCGGGCG CTAGGGCGCT GGCAAGTGTA GCGGTCACGC TGCGCGTAAC CACCACACCC GCCGCGCTTA ATGCGCCGCT ACAGGGCGCG TCCCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG CTATTACGCC AGCTGGCGAA AGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTG AATTGTAATA CGACTCACTA TAGGGCGAAT TGGAGCTCCA CCGCGGTGGC GGCCGCTCTA GAGTCCTGTA TTAGAGGTCA CGTGAGTGTT TTGCGACATT TTGCGACACC ATGTGGTCAC GCTGGGTATT TAAGCCCGAG TGAGCACGCA GGGTCTCCAT TTTGAAGCGG GAGGTTTGAA CGCGCAGCCA CCACGGCGGG GTTTTACGAG ATTGTGATTA AGGTCCCCAG CGACCTTGAC GGGCATCTGC CCGGCATTTC TGACAGCTTT GTGAACTGGG TGGCCGAGAA GGAATGGGAG TTGCCGCCAG ATTCTGACAT GGATCTGAAT CTGATTGAGC AGGCACCCCT GACCGTGGCC GAGAAGCTGC AGCGCGACTT TCTGACGGAA TGGCGCCGTG TGAGTAAGGC CCCGGAGGCC CTTTTCTTTG TGCAATTTGA GAAGGGAGAG AGCTACTTCC ACATGCACGT GCTCGTGGAA ACCACCGGGG TGAAATCCAT GGTTTTGGGA CGTTTCCTGA GTCAGATTCG CGAAAAACTG ATTCAGAGAA TTTACCGCGG GATCGAGCCG ACTTTGCCAA ACTGGTTCGC GGTCACAAAG ACCAGAAATG GCGCCGGAGG CGGGAACAAG GTGGTGGATG AGTGCTACAT CCCCAATTAC TTGCTCCCCA AAACCCAGCC TGAGCTCCAG TGGGCGTGGA CTAATATGGA ACAGTATTTA AGCGCCTGTT TGAATCTCAC GGAGCGTAAA CGGTTGGTGG CGCAGCATCT GACGCACGTG TCGCAGACGC AGGAGCAGAA CAAAGAGAAT CAGAATCCCA ATTCTGATGC GCCGGTGATC AGATCAAAAA CTTCAGCCAG GTACATGGAG CTGGTCGGGT GGCTCGTGGA CAAGGGGATT ACCTCGGAGA AGCAGTGGAT CCAGGAGGAC CAGGCCTCAT ACATCTCCTT CAATGCGGCC TCCAACTCGC GGTCCCAAAT CAAGGCTGCC TTGGACAATG CGGGAAAGAT TATGAGCCTG ACTAAAACCG CCCCCGACTA CCTGGTGGGC CAGCAGCCCG TGGAGGACAT TTCCAGCAAT CGGATTTATA AAATTTTGGA ACTAAACGGG TACGATCCCC AATATGCGGC TTCCGTCTTT CTGGGATGGG CCACGAAAAA GTTCGGCAAG AGGAACACCA TCTGGCTGTT TGGGCCTGCA ACTACCGGGA AGACCAACAT CGCGGAGGCC ATAGCCCACA CTGTGCCCTT CTACGGGTGC GTAAACTGGA CCAATGAGAA CTTTCCCTTC AACGACTGTG TCGACAAGAT GGTGATCTGG TGGGAGGAGG GGAAGATGAC CGCCAAGGTC GTGGAGTCGG CCAAAGCCAT TCTCGGAGGA AGCAAGGTGC GCGTGGACCA GAAATGCAAG TCCTCGGCCC AGATAGACCC GACTCCCGTG ATCGTCACCT CCAACACCAA CATGTGCGCC GTGATTGACG GGAACTCAAC GACCTTCGAA CACCAGCAGC CGTTGCAAGA CCGGATGTTC AAATTTGAAC TCACCCGCCG TCTGGATCAT GACTTTGGGA AGGTCACCAA GCAGGAAGTC AAAGACTTTT TCCGGTGGGC AAAGGATCAC GTGGTTGAGG TGGAGCATGA ATTCTACGTC AAAAAGGGTG GAGCCAAGAA AAGACCCGCC CCCAGTGACG CAGATATAAG TGAGCCCAAA CGGGTGCGCG AGTCAGTTGC GCAGCCATCG ACGTCAGACG CGGAAGCTTC GATCAACTAC GCAGACAGGT ACCAAAACAA ATGTTCTCGT CACGTGGGCA TGAATCTGAT GCTGTTTCCC TGCAGACAAT GCGAGAGAAT GAATCAGAAT TCAAATATCT GCTTCACTCA CGGACAGAAA GACTGTTTAG AGTGCTTTCC CGTGTCAGAA TCTCAACCCG TTTCTGTCGT CAAAAAGGCG TATCAGAAAC TGTGCTACAT TCATCATATC ATGGGAAAGG TGCCAGACGC TTGCACTGCC TGCGATCTGG TCAATGTGGA TTTGGATGAC TGCATCTTTG AACAATAAAT GATTTAAATA GTCATGTCTT TTGTTGATCA CCCTCCAGAT TGGTTGGAAG AAGTTGGTGA AGGTCTTCGC GAGTTTTTGG GCCTTGAAGC GGGCCCACCG AAACCAAAAC CCAATCAGCA GCATCAAGAT CAAGCCCGTG GTCTTGTGCT GCCTGGTTAT AACTATCTCG GACCCGGAAA CGGTCTCGAT CGAGGAGAGC CTGTCAACAG GGCAGACGAG GTCGCGCGAG AGCACGACAT CTCGTACAAC GAGCAGCTTG AGGCGGGAGA CAACCCCTAC CTCAAGTACA ACCACGCGGA CGCCGAGTTT CAGGAGAAGC TCGCCGACGA CACATCCTTC GGGGGAAACC TCGGAAAGGC AGTCTTTCAG GCCAAGAAAA GGGTTCTCGA ACCTTTTGGC CTGGTTGAAG AGGGTGCTAA GACGGCCCCT ACCGGAAAGC GGATAGACGA CCACTTTCCA AAAAGAAAGA AGGCTCGGAC CGAAGAGGAC TCCAAGCCTT CCACCTCGTC AGACGCCGAA GCTGGACCCA GCGGATCCCA GCAGCTGCAA ATCCCAGCCC AACCAGCCTC AAGTTTGGGA GCTGATACAA TGTCTGCGGG AGGTGGCGGC CCATTGGGCG ACAATAACCA AGGTGCCGAT GGAGTGGGCA ATGCCTCGGG AGATTGGCAT TGCGATTCCA CGTGGATGGG GGACAGAGTC GTCACCAAGT CCACCCGAAC CTGGGTGCTG CCCAGCTACA ACAACCACCA GTACCGAGAG ATCAAAAGCG GCTCCGTCGA CGGAAGCAAC GCCAACGCCT ACTTTGGATA CAGCACCCCC TGGGGGTACT TTGACTTTAA CCGCTTCCAC AGCCACTGGA GCCCCCGAGA CTGGCAAAGA CTCATCAACA ACTACTGGGG CTTCAGACCC CGGTCCCTCA GAGTCAAAAT CTTCAACATT CAAGTCAAAG AGGTCACGGT GCAGGACTCC ACCACCACCA TCGCCAACAA CCTCACCTCC ACCGTCCAAG TGTTTACGGA CGACGACTAC CAGCTGCCCT ACGTCGTCGG CAACGGGACC GAGGGATGCC TGCCGGCCTT CCCTCCGCAG GTCTTTACGC TGCCGCAGTA CGGTTACGCG ACGCTGAACC GCGACAACAC AGAAAATCCC ACCGAGAGGA GCAGCTTCTT CTGCCTAGAG TACTTTCCCA GCAAGATGCT GAGAACGGGC AACAACTTTG AGTTTACCTA CAACTTTGAG GAGGTGCCCT TCCACTCCAG CTTCGCTCCC AGTCAGAACC TGTTCAAGCT GGCCAACCCG CTGGTGGACC AGTACTTGTA CCGCTTCGTG AGCACAAATA ACACTGGCGG AGTCCAGTTC AACAAGAACC TGGCCGGGAG ATACGCCAAC ACCTACAAAA ACTGGTTCCC GGGGCCCATG GGCCGAACCC AGGGCTGGAA CCTGGGCTCC GGGGTCAACC GCGCCAGTGT CAGCGCCTTC GCCACGACCA ATAGGATGGA GCTCGAGGGC GCGAGTTACC AGGTGCCCCC GCAGCCGAAC GGCATGACCA ACAACCTCCA GGGCAGCAAC ACCTATGCCC TGGAGAACAC TATGATCTTC AACAGCCAGC CGGCGAACCC GGGCACCACC GCCACGTACC TCGAGGGCAA CATGCTCATC ACCAGCGAGA GCGAGACGCA GCCGGTGAAC CGCGTGGCGT ACAACGTCGG CGGGCAGATG GCCACCAACA ACCAGAGCTC CACCACTGCC CCCGCGACCG GCACGTACAA CCTCCAGGAA ATCGTGCCCG GCAGCGTGTG GATGGAGAGG GACGTGTACC TCCAAGGACC CATCTGGGCC AAGATCCCAG AGACGGGGGC GCACTTTCAC CCCTCTCCGG CCATGGGCGG ATTCGGACTC AAACACCCAC CGCCCATGAT GCTCATCAAG AACACGCCTG TGCCCGGAAA TATCACCAGC TTCTCGGACG TGCCCGTCAG CAGCTTCATC ACCCAGTACA GCACCGGGCA GGTCACCGTG GAGATGGAGT GGGAGCTCAA GAAGGAAAAC TCCAAGAGGT GGAACCCAGA GATCCAGTAC ACAAACAACT ACAACGACCC CCAGTTTGTG GACTTTGCCC CGGACAGCAC CGGGGAATAC AGAACCACCA GACCTATCGG AACCCGATAC CTTACCCGAC CCCTTTAACC CATTCATGTC GCATACCCTC AATAAACCGT GTATTCGTGT CAGTAAAATA CTGCCTCTTG TGGTCATTTC TAGAACTAGA ACTAGTGGAT CCCCCGGGCT GCAGGAATTC GATATCAAGC TTATCGATAC CGTCGACCTC GAGGGGGGGC CCGGTACCCA GCTTTTGTTC CCTTTAGTGA GGGTTAATTT CGAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGC
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