pENTR223-LYN
$84.00
In stock
SKU
PL83874
NM_002350.3;JTK8;p53Lyn;p56Lyn
100μg liquid plasmid(Instantly):$105
| name | pENTR223-LYN | another name | apENTR223-LYN |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Gene template | Fragment type | cDNA |
| Fragment species | Human | Prokaryotic resistance | Spe |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | M13F：TGTAAAACGACGGCCAGT | |
| 3′ sequencing primer | M13R：CAGGAAACAGCTATGACC | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC CTGTTCGTTG CAACAAATTG ATAAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA AGTTGGCATG GGATGTATAA AATCAAAAGG GAAAGACAGC TTGAGTGACG ATGGAGTAGA TTTGAAGACT CAACCAGTAC GTAATACTGA AAGAACTATT TATGTGAGAG ATCCAACGTC CAATAAACAG CAAAGGCCAG TTCCAGAATC TCAGCTTTTA CCTGGACAGA GGTTTCAAAC TAAAGATCCA GAGGAACAAG GAGACATTGT GGTAGCTTTG TACCCCTATG ATGGCATCCA CCCGGACGAC TTGTCTTTCA AGAAAGGAGA GAAGATGAAA GTCCTGGAGG AGCATGGAGA ATGGTGGAAA GCAAAGTCCC TTTTAACAAA AAAAGAAGGC TTCATCCCCA GCAACTATGT GGCCAAACTC AACACCTTAG AAACAGAAGA GTGGTTTTTC AAGGATATAA CCAGGAAGGA CGCAGAAAGG CAGCTTTTGG CACCAGGAAA TAGCGCTGGA GCTTTCCTTA TTAGAGAAAG TGAAACATTA AAAGGAAGCT TCTCTCTGTC TGTCAGAGAC TTTGACCCTG TGCATGGTGA TGTTATTAAG CACTACAAAA TTAGAAGTCT GGATAATGGG GGCTATTACA TCTCTCCACG AATCACTTTT CCCTGTATCA GCGACATGAT TAAACATTAC CAAAAGCAGG CAGATGGCTT GTGCAGAAGA TTGGAGAAGG CTTGTATTAG TCCCAAGCCA CAGAAGCCAT GGGATAAAGA TGCCTGGGAG ATCCCCCGGG AGTCCATCAA GTTGGTGAAA AGGCTTGGCG CTGGGCAGTT TGGGGAAGTC TGGATGGGTT ACTATAACAA CAGTACCAAG GTGGCTGTGA AAACCCTGAA GCCAGGAACT ATGTCTGTGC AAGCCTTCCT GGAAGAAGCC AACCTCATGA AGACCCTGCA GCATGACAAG CTCGTGAGGC TCTACGCTGT GGTCACCAGG GAGGAGCCCA TTTACATCAT CACCGAGTAC ATGGCCAAGG GCAGTTTGCT GGATTTCCTG AAGAGCGATG AAGGTGGCAA AGTGCTGCTT CCAAAGCTCA TTGACTTTTC TGCTCAGATT GCAGAGGGAA TGGCATACAT CGAGCGGAAG AACTACATTC ACCGGGACCT GCGAGCAGCT AATGTTCTGG TCTCCGAGTC ACTCATGTGC AAAATTGCAG ATTTTGGCCT TGCTAGAGTA ATTGAAGATA ATGAGTACAC AGCAAGGGAA GGTGCTAAGT TCCCTATTAA GTGGACGGCT CCAGAAGCAA TCAACTTTGG ATGTTTCACT ATTAAGTCTG ATGTGTGGTC CTTTGGAATC CTCCTATACG AAATTGTCAC CTATGGGAAA ATTCCCTACC CAGGGAGAAC TAATGCCGAC GTGATGACCG CCCTGTCCCA GGGCTACAGG ATGCCCCGTG TGGAGAACTG CCCAGATGAG CTCTATGACA TTATGAAAAT GTGCTGGAAA GAAAAGGCAG AAGAGAGACC AACGTTTGAC TACTTACAGA GCGTCCTGGA TGATTTCTAC ACAGCCACGG AAGGGCAATA CCAGCAGCAG CCTTTCCCAA CTTTCTTGTA CAAAGTTGGC ATTATAAGAA AGCATTGCTT ATCAATTTGT TGCAACGAAC AGGTCACTAT CAGTCAAAAT AAAATCATTA TTTGCCATCC AGCTGATATC CCCTATAGTG AGTCGTATTA CATGGTCATA GCTGTTTCCT GGCAGCTCTG GCCCGTGTCT CAAAATCTCT GATGTTACAT TGCACAAGAT AAAAATATAT CATCATGCCT CCTCTAGACC AGCCAGGACA GAAATGCCTC GACTTCGCTG CTGCCCAAGG TTGCCGGGTG ACGCACACCG TGGAAACGGA TGAAGGCACG AACCCAGTGG ACATAAGCCT GTTCGGTTCG TAAGCTGTAA TGCAAGTAGC GTATGCGCTC ACGCAACTGG TCCAGAACCT TGACCGAACG CAGCGGTGGT AACGGCGCAG TGGCGGTTTT CATGGCTTGT TATGACTGTT TTTTTGGGGT ACAGTCTATG CCTCGGGCAT CCAAGCAGCA AGCGCGTTAC GCCGTGGGTC GATGTTTGAT GTTATGGAGC AGCAACGATG TTACGCAGCA GGGCAGTCGC CCTAAAACAA AGTTAAACAT CATGAGGGAA GCGGTGATCG CCGAAGTATC GACTCAACTA TCAGAGGTAG TTGGCGTCAT CGAGCGCCAT CTCGAACCGA CGTTGCTGGC CGTACATTTG TACGGCTCCG CAGTGGATGG CGGCCTGAAG CCACACAGTG ATATTGATTT GCTGGTTACG GTGACCGTAA GGCTTGATGA AACAACGCGG CGAGCTTTGA TCAACGACCT TTTGGAAACT TCGGCTTCCC CTGGAGAGAG CGAGATTCTC CGCGCTGTAG AAGTCACCAT TGTTGTGCAC GACGACATCA TTCCGTGGCG TTATCCAGCT AAGCGCGAAC TGCAATTTGG AGAATGGCAG CGCAATGACA TTCTTGCAGG TATCTTCGAG CCAGCCACGA TCGACATTGA TCTGGCTATC TTGCTGACAA AAGCAAGAGA ACATAGCGTT GCCTTGGTAG GTCCAGCGGC GGAGGAACTC TTTGATCCGG TTCCTGAACA GGATCTATTT GAGGCGCTAA ATGAAACCTT AACGCTATGG AACTCGCCGC CCGACTGGGC TGGCGATGAG CGAAATGTAG TGCTTACGTT GTCCCGCATT TGGTACAGCG CAGTAACCGG CAAAATCGCG CCGAAGGATG TCGCTGCCGA CTGGGCAATG GAGCGCCTGC CGGCCCAGTA TCAGCCCGTC ATACTTGAAG CTAGACAGGC TTATCTTGGA CAAGAAGAAG ATCGCTTGGC CTCGCGCGCA GATCAGTTGG AAGAATTTGT CCACTACGTG AAAGGCGAGA TCACCAAGGT AGTCGGCAAA TAACCCTCGA GCCACCCATG ACCAAAATCC CTTAACGTGA GTTACGCGTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCATT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCA
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