pBC1
$128.00
In stock
SKU
PL83761
山羊乳特异性乳汁
100μg liquid plasmid(Instantly):$149
| name | pBC1 | another name | apBC1 |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | ||
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TCGACTCTAG AGGGACAGCC CCCCCCCAAA GCCCCCAGGG ATGTAATTAC GTCCCTCCCC CGCTAGGGGC AGCAGCGAGC CGCCCGGGGC TCCGCTCCGG TCCGGCGCTC CCCCCGCATC CCCGAGCCGG CAGCGTGCGG GGACAGCCCG GGCACGGGGA AGGTGGCACG GGATCGCTTT CCTCTGAACG CTTCTCGCTG CTCTTTGAGC CTGCAGACAC CTGGGGGGAT ACGGGGAAAA AGCTTTAGGC TGAAAGAGAG ATTTAGAATG ACAGAATCAT AGAACGGCCT GGGTTGCAAA GGAGCACAGT GCTCATCCAG ATCCAACCCC CTGCTATGTG CAGGGTCATC AACCAGCAGC CCAGGCTGCC CAGAGCCACA TCCAGCCTGG CCTTGAATGC CTGCAGGGAT GGGGCATCCA CAGCCTCCTT GGGCAACCTG TTCAGTGCGT CACCACCCTC TGGGGGAAAA ACTGCCTCCT CATATCCAAC CCAAACCTCC CCTGTCTCAG TGTAAAGCCA TTCCCCCTTG TCCTATCAAG GGGGAGTTTG CTGTGACATT GTTGGTCTGG GGTGACACAT GTTTGCCAAT TCAGTGCATC ACGGAGAGGC AGATCTTGGG GATAAGGAAG TGCAGGACAG CATGGACGTG GGACATGCAG GTGTTGAGGG CTCTGGGACA CTCTCCAAGT CACAGCGTTC AGAACAGCCT TAAGGATAAG AAGATAGGAT AGAAGGACAA AGAGCAAGTT AAAACCCAGC ATGGAGAGGA GCACAAAAAG GCCACAGACA CTGCTGGTCC CTGTGTCTGA GCCTGCATGT TTGATGGTGT CTGGATGCAA GCAGAAGGGG TGGAAGAGCT TGCCTGGAGA GATACAGCTG GGTCAGTAGG ACTGGGACAG GCAGCTGGAG AATTGCCATG TAGATGTTCA TACAATCGTC AAATCATGAA GGCTGGAAAG CCTCCAAGAT CCCCAAGACC AACCCCAACC CACCCACCGT GCCCACTGGC CATGTCCCTC AGTGCCACAT CCCCACAGTT CTTCATCACC TCCAGGGACG GTGACCCCCC CACCTCCGTG GGCAGCTGTG CCACTGCAGC ACCGCTCTTT GGAGAAGGTA AATCTTGCTA AATCCAGCCC GACCCTCCCC TGGCACAACG TAAGGCCATT ATCTCTCATC CAACTCCAGG ACGGAGTCAG TGAGGATGGG GCTCTAGAGG GACAGCCCCC CCCCAAAGCC CCCAGGGATG TAATTACGTC CCTCCCCCGC TAGGGGCAGC AGCGAGCCGC CCGGGGCTCC GCTCCGGTCC GGCGCTCCCC CCGCATCCCC GAGCCGGCAG CGTGCGGGGA CAGCCCGGGC ACGGGGAAGG TGGCACGGGA TCGCTTTCCT CTGAACGCTT CTCGCTGCTC TTTGAGCCTG CAGACACCTG GGGGGATACG GGGAAAAAGC TTTAGGCTGA AAGAGAGATT TAGAATGACA GAATCATAGA ACGGCCTGGG TTGCAAAGGA GCACAGTGCT CATCCAGATC CAACCCCCTG CTATGTGCAG GGTCATCAAC CAGCAGCCCA GGCTGCCCAG AGCCACATCC AGCCTGGCCT TGAATGCCTG CAGGGATGGG GCATCCACAG CCTCCTTGGG CAACCTGTTC AGTGCGTCAC CACCCTCTGG GGGAAAAACT GCCTCCTCAT ATCCAACCCA AACCTCCCCT GTCTCAGTGT AAAGCCATTC CCCCTTGTCC TATCAAGGGG GAGTTTGCTG TGACATTGTT GGTCTGGGGT GACACATGTT TGCCAATTCA GTGCATCACG GAGAGGCAGA TCTTGGGGAT AAGGAAGTGC AGGACAGCAT GGACGTGGGA CATGCAGGTG TTGAGGGCTC TGGGACACTC TCCAAGTCAC AGCGTTCAGA ACAGCCTTAA GGATAAGAAG ATAGGATAGA AGGACAAAGA GCAAGTTAAA ACCCAGCATG GAGAGGAGCA CAAAAAGGCC ACAGACACTG CTGGTCCCTG TGTCTGAGCC TGCATGTTTG ATGGTGTCTG GATGCAAGCA GAAGGGGTGG AAGAGCTTGC CTGGAGAGAT ACAGCTGGGT CAGTAGGACT GGGACAGGCA GCTGGAGAAT TGCCATGTAG ATGTTCATAC AATCGTCAAA TCATGAAGGC TGGAAAGCCT CCAAGATCCC CAAGACCAAC CCCAACCCAC CCACCGTGCC CACTGGCCAT GTCCCTCAGT GCCACATCCC CACAGTTCTT CATCACCTCC AGGGACGGTG ACCCCCCCAC CTCCGTGGGC AGCTGTGCCA CTGCAGCACC GCTCTTTGGA GAAGGTAAAT CTTGCTAAAT CCAGCCCGAC CCTCCCCTGG CACAACGTAA GGCCATTATC TCTCATCCAA CTCCAGGAAC GGAGTCAGTG AGGATGGGGC TCTAGAGGAT CCCTCGACCT GCAGGTCAAC GGATCACAAC AAACTGGAAA ATTCTTCAAG AGAAGAATAC CAGACCACCC TACCTGCTTC CTGAGAAATC TGTTTGCTGC TCAGAAGCAA CAGTTAGAAC CAGACATGGA ACAACAGACT GGTTCCAAAT CAGGAAAGGA GTATGTCAAG GCTGTATATC GTCACCCTGA TTATTTAACT TATATGCATA GTACATAATA CAAAATGCCA GGCTGGATGA ATCGCAAGCT GGAATCAAGA TTTCTGGGAG AAATATCAAT AAACGAGATA CAAAGATACA CCACACTTAT GGCAGAAAAC TAAGAAGAAC TAAAGAGCCT CTTGATGAAA GTGAAAGAGG AGAGTGAAAA AGCCAGCTTA AAACCCAACA TTCAAAATCA AGATCATCAT TTCATGGCAA ATAAATGGGG AAACAATGGA AACAGTGAGA GACTTTATTT TCTTGGGCTC CAAAATCACT GCAGATTGTG ACTACAGCCA TGATTAAAAG ATGCTTGCTC CTTGGAAGAG AAGCTATTAC CAAACTAGAA AGCATATTAA AAAGCAGAGA CGTTACTTTG CTGACTAAGT TCTGTCTAGT CAAACCTATG GTTTTTCCAG TAGTCATATA TGGATGTGAG TTGAACTATA AAGAAAGCTG AGCACCAAAG AATTGATGCT TTTGAAATTT GGTGTTGGAG AAGTCTCTTG AGAGTCCCTT GAACCTGCAA GGAGATCCAA CCAGTCCATC CTAAAGGAAA TCAGTCCTGA ATATTCATTG GAAGGACTGA TGCTGAAATT GAAGATTAAC GTTTTGGACT CACCTAATGC AGAAGAGCCA ACTCACTAGA AAAGACCCCA TGTTGGCAAA AATTGAAGCC AGGAAGAGAA GTGAATGACA GAGGATGAGA TGGTTGGATG GCATCGTTGA CTGAATGGAC ATGAGTCTGA TCAAGTTCCG GGAGACAGCA AAGGACAGGG CTGCCTGGTC TGCTGCAGTC CATGGGGTTG CAAAGAGTCG GTCTCAAATG AGTAACTAAA CAACAACCAA GCAGTAGAAA AATAAATAAA ATTTGTCTCT GAGATCTCAG TACCTCTTTC TGTGCATATC CGTCTCCTGT TATTGTACTT TGTCTTCTGC TTGTAATAAA GCTGTCCTGT TAGTAAAATC TGTTTGGGTC CTCTGAATTC TTTTAGCTAT CAAAAATGGA AGGTGATTAT TGTGCAATGT CCACCTCTGA GTAATATACA GAGAATAAAA GAAGGGAGAA ATTATGTGCA AGTTCTCTCT CATCTCCTGC TTCTCATTTA AAAGATTCTA CCTCAGTGGG GGCTAAAACT CCACATTTAA CAGTAGCAAA AACCAATATT CCATAGCTTC TTAGGAAACC ATTTTTTATA CTCTTGTATG TAATTACATT CAAGCTCAAA AGCAAAGAAG TGATTCTGCG TTGGTGAAGG CCCAACCATA GAAAAGAGGA AGAAAATAGG CCACATACTG TGCTTCCCCC ATAGCTCAGT TGGTAAAGAA TCTACCTACA ATGCAGGAGG CCTGGGCTTG ATCCCTGGGT AAGGGAGATC CCCTGGAGAA GGAAATGGTA ACCCACTCCA GTACTCTTGC CTGTAAATCC CATGGACGGA GGAGCCTGGC AGCTACAGCC TTGGGGTGGC AAGAGTTGGA CATGATTAAC AACTAAACCA CTGCCACCAC TCCACATACT GAGTGCTCCC CAGTGGCACT AGTGGTAAAG AACCACCTGC CGGTGCAGAA GACATTAAAG ACACTGGCTC TATCCCTGCT TGGGAAGTAG GGAAGATCCC CTAGAGAGGG AAATAGCAAC CCACTCCAGA ATTCTTGCCT GGAAAATCCC ATGAATGAAG ACTGGCGGGC TGTAGTAACT GGGGTCACAA AGAGTTAAAC ATGATTTAGC AACTAAACAT CACCACATTA AAAAAATTAC CACCAAAATA GTCATATTCC AGGCTAAGGG GAATAATAGC ACTAGTACCT GAGAGAACTT TCTCAGATTC TCTGTCAAGT TCTTCCTTCT CTCATATAAC CAGTAGTCTA GTTTACCTCA TCAGATATTA ACTACTCATC GATTCTAAAT TATCTAATTA TGGGGGGGGG CACTACATTG CATTATATTT TGTGTCCATT GACTATCACT CAATTTATTT ATAAAAAATT CATCCATGTT GTTTCTGTGA CAGTAACTCA TTCACATTAA TTGTAATATC TCATTGCATT GTATACTACA ATTTATTTAT ACAAAATACT ATTATTCACA CTTCTGTTGA TTTTAATTTG GAACATCAAC AATAACGTGG CTGAGAAGCT TCTTTCTTTA GTATATTGTT AAGGATTTCC TTGATCAAGA TTTTACCTAC TTTTCTGGTC CAATTGGTGA GAGACAGTCA TAAGGAAATG CTGTGTTTAT TGCACAATAT GTAAAGCATC TTCCTGAGAA AATAAAAGGG AAATGTTGAA TGGGAAGGAT ATGCTTTCTT TTGTATTCCT TTTCTGAGAA ATCAGACTTT TTCACCTTGG CCTTGGCCAC CAAAAGCTAA CAAATAAAGG CATATGAAGT AGCCAAGGCC TTTTCTAGTT ATATCTATGA CACTGAGTTC ATTTCATCAT TTATTTTCCT GACTTCCTCC TGGGTCCATA TGAGCAGTCT TAGAATGAAT ATTAGCTGAA TAATCCAAAT ACATAGTAGA TGTTGATTTG GGTTTTCTAA GCAATCCAAG ACTTGTATGA CAGTAAGATG TATTACCATC CAACACACAT CTCAGCATGA TATAAATGCA AGGTATATTG TGAAGAAAAA TTTTTAATTA TGTCAAAGTG CTTACTTTAG AAGGTCATCT ATCTGTCCCA AAGCTGTGAA TATATATATT GAAGGTAATG AATAGATGAA GCTAACCTTG TAAAAATGAG TAGTGTGAAA TACAACTACA ATTATGAACA TCTGTCACTA AAGAGGCAAA GAAACTTGAA GATTGCTTTT GCAAATGGGC TCCTATTAAT AAAAAGTACT TTTGAGGTCT GGCTCAGACT CTATTGTAGT ACTTAGGGTA AGACCCTCCT CCTGTATGGG CTTTCATTTT CTTTCTTGCT TCCCTCATTT GCCCTTCCAT GAATACTAGC TGATAAACAT TGACTATAAA AGATATGAGG CCAAACTTGA GCTGTCCCAT TTTAATAAAT CTGTATAAAT AATATTTGTT CTACAAAAGT ATTATCTAAA TAAATGTTAC TTTCTGTCTT AAAATCCCTC AACAAATCCC CACTATCTAG AGAATAAGAT TGACATTCCC TGGAATCACA GCATGCTTTG TCTGCCATTA TCTGACCCCT TTCTCTTTCT CTCTTCTCAC CTCCATCTAC TCCTTTTTCC TTGCAATTCA TGACCCAGAT TCACTGTTTG ATTTGGCTTG CATGTGTGTG TGCTGAGTTG CGTCTGACTG TTATCAACCC CATGAATGAT AGTCCACCAG GCTCTACTGT CCATGAAATT TTCCAGTCAA GAATACTGGA GTGGATTGCA TTTCCTACTC CATTTGATTA ATTTAGTGAC TTTTAAATTT CTTTTTCCAT ATTCGGGAGC CTATTCTTCC TTTTTAGTCT ATACTCTCTT CACTCTTCAG GTCTAAGGTA TCATCGTGTG CTTGTTAGCT TGTTACTTTC TCCATTATAG CTTAAGCACT AACAACTGTT CAGGTTGGCA TGAAATTGTG TTCTTTGTGT GGCCTGTATA TTTCTGTTGT GTATTAGAAT TTACCCCAAG ATCTCAAAGA CCCACTGAAT ACTAAAGAGA CCTCATTGTG GTTACAATAA TTTGGGGACT GGGCCAAAAC TTCCGTGCAT CCCAGCCAAG ATCTGTAGCT ACTGGACAAT TTCATTTCCT TTATCAGATT GTGAGTTATT CCTGTTAAAA TGCTCCCCAG AATTTCTGGG GACAGAAAAA TAGGAAGAAT TCATTTCCTA ATCATGCAGA TTTCTAGGAA TTCAAATCCA CTGTTGGTTT TATTTCAAAC CACAAAATTA GCATGCCATT AAATACTATA TATAAACAGC CACTAAATCA GATCATTATC CATTCAGCTT CTCCTTCACT TCTTCTCCTC TACTTTGGAA AAAAGGTAAG AATCTCAGAT ATAATTTCAG TGTATCTGCT ACTCATCTTT ATTTTGGACT AGGTTAAAAT GTAGAAAGAA CATAATTGCT TAAAATAGAT CTTAAAAATA AGGGTGTTTA AGATAAGGTT TACACTATTT TCAGCAGATA TGTTAAAAAA TAGAAGTGAC TATAAATACT TGATAAAAAT TATAGTGACT GCAAATGTTT TAGGAATATA ATAAGATATA ATAACAGTGG TTGCTATTTT CTTTAGCACA AGACTAGTTA ACAGGCTGTA TTAAAAGATC TTTTCTTGAA TTAAATATTT TCAATTTGAT TAAACCTACC TCAGCCATAA AGGCAAGCAC ATTTCATTTA TACTATGGGG ATTTGAATAA TTATTACTGA AGAAGCTCTA CCAACAAAAA GTTTATAGAG CTATCATATT TAGTCAAGAG ATAAAGAGGG TTGTTAGGAT ATATATGCTA TTTGAAAGGT ATTTATAAAA GAAGAGTATA TTTATCAAAA TTTCTCAAGA ACATCCAAAT TTCAAGTTTA TCATTTATCT TACAATATTT CAAAAATATT AAAATAGATA CATGAAATAC AGAAGTAAAT TAAAGAGAAA GTATTTTACT TGGTAAAAAA ATTCTAGGTT GGACAGAGAG TGCCAGGAAA CAAAAACAAT GAAAAATGTG ACCTGACAGG AATTATAGCT CAAAGTATAG TAGTAAGTAA TGAAATGGCT TAAAAATTGG TATATAAAAT GCTAGTTATA AAATAAACAA AATGCAATAA TATCCTCCCT ACATGTAATG AATTCTAGGT ATTATGATTA TGCTCTTTTT TGAAGTCTTG ACAATAAAAA TTTTTTTAGA AGTTTATAGG CATCTTGAAT AAAGTGAAAC AAATTAAGAA TTAGTATCCA TGAGAAAAAT ATAGAACAAT TTTCCTAATT TAGTTTGAAA ATCTGGGATT GAAGATGTGT GTCAAGAGAT GTTGGTGGCA AGAACATTTT TTTTTCAAGA ACTTATAAAA ATGCAACAAA ACAAACCATT TAATACATTT TGGTCAAAAT CAATAATGTA TTTTATTTTA TGCTCCAAGG AGCATAAAAT TGGGGACTGG GCAAGAGAAA CTGACACCCT GGTAAATTAC CAAGAGATAA GTACACAGTT ACTATAGTAG AAAATAAGCA TAGTGTATGA TCTCTAAAAT TATGTGAGAC AAAGGAGAGA TGACATTAGG CATGTGGGGA TGAAGACTGA GTAGAGAAGA AACAATCTAA TCAGTCCAAG AAAACATCTC GATCAGTGGA ACAAATAGAA GAAATGCTAA AATGAAACAG AAGTCTTACT GGAAATAAAA GATATGCATA AGACAAAAAT TCATGAAAAT CACTTAGTTT AGCAGAGAAA AGATAAAAAT AAAGTATGAC CTTCTTCATA TACATTGTTT GATCATATGC ACCTCAATAA AACTGAGTCT CCAACAGAAA TGAAACATTA ATATTTTGTT CACTGCTCTA ATCCCAGAAT CTAAGCGATA TCTGGCAATA AAAATAATAA ATATATATTT TTTAATAAAT GAATCAACCA CTTAATTTTT CTGTAAATAT CTGTAACTTC TCTTCTGTCT TTCCAAAAAC ACTCATAAGT ACTGTGAATG AGATGAAAAA GAGTGAAGTA GGATATAGGC TGTTAGCAGA AAACATCTGA ATGGCTGGCA GTGAAACATT AACTTGAAAT GTAAGATTAA TGAGTAATAG TAAATTTTAA CCTTGGCCAT ATGATAAAAT GTTCATTAAT ATTTTTCTAG AATACAGGGC TTTTTGTTTT TGCCATGAGG TTTGCAGGAT CTTGGTTCCC TGACCAGGGA TCAAACCTGC ACACCAGGGA TCAAACCTGC ACTCCCCTGG AAGCATGGAG TCTTGGACAT TTGTATTATA CACTATCTTT GGTTCCTTTT AAAGGGAAGT AATTTTACTT AAATAAGAAA ATAGATTGAC AAGTAATACG CTGTTTCCTC ATCTTCCCAT TCACAGGAAT CGCGGATCCT CGAGGATCCG GACCCTTCCC TATTCTTGTA AGTCTAAATT TACTAACTGT GCTGTTTAAC TTCTGATGTT TGTATGATAT TTGAGTAATT AAGAGCCCTA CAAAAAAATC AATAATGAAT GGTTCCAAAA TAAGCATAGC TGAGATTAAT GATTCTCAGC ATTAGTTATA AATAGAATAA GCTGGAAAAC CTTCACCTCC CCTCCACCAC CAGATCTCAA TGTCTAGGCT TACCCATGGA GATTCTGATT AACTGTTCTT TCTATGTAGA AGAAACTTAT TGGGAAGAAA TAATATAATG GACTATGATT TAATTGGTCT GTTGAGAATT TAGATGAAGG GGATTAAGTT ACAATAAAGC CAGAATTTAA CTTGATAATC TCATTTGGCT AAGAATAACA AACCTAAGAA GGTTTGCTAT TTTCTACAAT TTTGAAGTTT TCCTTATGCA CAATTATTTC ACCACATGAC TCATTTCACA TCTTGTTTTT GATATATGAG CATATGAGGG CAAAATACTG AAGATGCTTA TTTCAATACT CAGGGAAAAT TTTCTTGCCA AAAGGCAAGA ATTGTATAAT TCATTCACTT ATTTTATTTT TTTTAATTTT TAAGGTCTAA GAGGATTTCA AAGTGAATGC CCCCTCCTCA CTTTTGGTAA GCTTTAGGAG ATTGGAGGCA GACTGATCAT TTTTATAGTT AATATCTTTT ACATTTCATC TTCCTGGATA AGCCCCAATA GTAGCAATTT CTATCAGTAT ACCAGCATAA AGATTAGTTT TAAATTTATT TTCAGTGATT GACTGTTATT TACTGACCTG AAATTATGTA TCTGTTATAT TTCAAATAAT GCAAAACTGT ATATATATGG TGTTGACAGA TTTGATTGGT TTTCTTTCAA TTGCCTATAT CCTTATTATT GATTGTAATC ATTTATAGAA AAAACAAAAT AATTTCTTAT ACTTTTATGT AAACCTGTTA GAGCTTATTT TAAAGATCAA CTGCATTCAC ATTTCTAATC TAGTCATTAT GAGCTTCAAT TGTTTTATCT CACTTAAAAT TTATATATTG TCTTTTAATT CATGAGTCAA AATACAATCT CACAGTCCAG ATATGGGACT TAAAAGGGGA ATAGCATATA GTTTTGATAT TCTTAAAGAT ATACATCTTT TTGTGATCAT GATTCAGCAG ACATTTTAAT AAAACAATTC CAAGTGAGCC GACACTTGGT CCTAGAGGAA TTTTTATAAC CTTAAGATAA GGCACAGCAT GGTGTTTTTG TAATAAGATT TCTTTTATGA AAAAGTCACA CCAAAATTGG AAATGGGGTG AGATGAAGAG TTATAACATA TAACTAAATG GACATTTGTT CTCTATTCCA CAGAATTGAC TGCGACTGGA AATATGGCAA CTTTTCAATC CTTGCATCAT GCTACTAAGA TAATTTTTAA ATGAGTATAC ATGGAACAAA AAATGAAACT TTATTCCTTT ATTTATATTA TGCTTTTTCA TCTTAATTTG AATTTGAGTC ATAAACCATA TACTTTCAAA ATGTTAATTC AACATTAGCA TAAAAGTTCA ATTTTAACTT GGAAATATCA TGAACATATC AAATTATGTA TAAAAATAAT TTCTGGAATT GTGATTATTA TTTCTTTAAG AATCTATTTC CTAACCAGTC ATTTCAATAA ATTAACCCTT AGGCATATTT AAGTTTTCTT GTCTTTATTA TATTTTTAAA AATGAAATTG GTCTCTTTAT TGTTAACTTA AATTTATCTT TGATGTTAAA AATAGCTGTG GAAAATTAAA ATTGAATAGA ATTCTTTGAA TTGAGTTCCA AAGGATATCA AAAAGTGAGG GAAAAGATAG GGTGAGCCTA TGCTGCATAT GTCCTTAGAA AGTCTTGGTT TATACCTGTT ACCTAAGTTA AACAATTATA CTTGTTCCTT TCACTCTCGA AAGTACCCAG CATTGGATGT TAAATTTTAT AGTCATCCTA GACAAAAAAA AAAAAAAAAA CAAACAACCC TCAAATGTGA TATCTGAATC ACAGCTCTAC AGTGTGGTAG CTAAGTGGTG CTGTGTAAGT TAGTCTCCAA GAGATTCCAT TTCTACATTT ATAAACAGTC AATTTAAGGT GTTTTATTGA AGTTTTAATG TGAAAAGTGC ACTATATGGT GCATGATAGG AGTTCCTGGT TGAATCTCAT TTCTGACATC ACTGACACCA GTGCAGCAAG GACTAGTGTT ACAATCAGAA GGAGCTGAGT TGTGTAATTT TAGCCATTAA TGCCCAAGAG ACTAGAACTT ACACAAAGCT CTAATATCCA TTGTCTCTGT CTGTGGAGTA ATTATTTCAT TGCCATGAAT TATCTGTCTG TCATATCCTG CATTTTTATA CATGATTCAG TTCCCTTCAG TTCACACAAT GACTTGTCTA ATTTCATCTT TCCTGCATCC TCCATGTTTT CCTCACTTCA GGATTAAGTG AAGCCGTACT TAGGCACAAT ATTTCTTATC TTTAAAGAAA AATTCCATCT TTGAGAGTTG TTATTGTTCA GTCACTAGGT CATGTCCAAC TCTTTGTGAC CCCATGCACT GCAGCATGCC AGGCTTCCCT GCCCTTCGCT CTCTCCTGGA GTTTGCTCAG ACTCATGTAG ATTGAGTCGG TGATGGTATC CAACTATCTC ATCAACTGTT GTGCCCTTCT CCTCCTACCC TCAGTCTTTA CCAGCATCAG AGTCTTTCTC AGATTCTTCA GGTTATTATA TAACAACTAT CATAAAAGGA GTATCTAAAT GGCTGTGTCC ATTATTTCAC ATGTTATTCT CTCTTTAACT TGCTCCAATC CCAATTTTAT CCCTATGGGA ACTGCTTTAT TGAAGATCAC CAACAACTTT TATTTTACTA ATCGTTTTGT TTTACCCAAC CTCTCAGTGA GTGTTATGAG GTAGAGTTGA CTATTTCTTC ATTTTGAAAT ATTACGCTTC ATTTCATTTG ATATCCTAAA GCTCATAAGG TGTGGTTTTT CTCTTAACTC ACTAGACACT TCTTTGAAGT CTCTCTTCTG GCATTTTCTC CTTTTCCAAA ATTTTAATGG TTGGAGTACC CTAGATTTTA GCCTTAATTT GTTTGATGTT GTTCAGTTCC ATTCTCAGCT CAGAGCTTCC AACTGTATGT CTCCAAACTT ACTCGTTTTG TAAACTCCAA ACTCATGCAC TCAACTGCAT TCTTGACCTC CACACTGAAT TATCTAATTA ATGTCCTAAA TCTGGCATGA CCAAGCATAC ATTTTTGTCT GAATCCAGTC CCCAACTTGC TCAAAATTTA ATTAAACGTA ATTCAGTTAC AAAGGCAGCT GATATTGTAT GCAATAGACC TGAATGGGAA CTTCACAAAA GAAGTTATCT TAATTGTCAA TAAAAACATG AAAAATACTC TACATCATCA ATCTTCAGAA AAATGCAAAT TAAAGGTGCC TAATAATATC ATGACACAAC CGTCAGAATG ACTGAAATGA AAAGAATTGT AATACAGTTC AGTTCAGTTC AGTTACTCAG TCGTCTCCAA CTCTTTGTGA CCCCATGAAC TGCAGCATGA CAGACCTTCC TGTCCATCAC CAACTCCCAG AGTTTACTCA GACTATGTCC ATTGAGTTGA TGATGCCATC CAACCATCTC ATCCTCTGTC GTCCCCTTCT CCTCCTGCCC TCAGTCTTTC CCAGCATCAG GGTCTTTTCC AATGAGTCAG CTCTTCGCAT CAGGTGGCTA AAGTATTGGA GTTTCAGCTT CAACATCAGT CCTTCTAATT AACACCCAGG ACTGATCTCT TTTAGGATGG ACTAGTTGGA TCTCCTTGCA GTCCAAGGGA CTCTCAAGAG TCTTCTCCAA CACCACAGTT CAAAAGCATC AATTCCTTGG CACTCAGCTT TCCTTATAGT CCATGTCTCA CATCCACACA TGACTATTGG AAAAACCATA GCCTTGACTA GGTGGACCTT TGTTGACAAA GTAATGTCTC TGCTTTTTAA TATGTTGTCT AGATTGGTCA TAACTTTCCT TCCAAGAAGT AATTGTCTTT TAATTTCATG GCTGCAGTCA CCATCTGCAG TGATTTTGGA GCCCCAAAAT ATAAAGTCAG CTGCTGTTTC CACTGTTGCC CCATCTACCC CATCTATTTG CCATGAAGTG ATGGGACTGG ATGCCACTAT CTTAGTTTTC TGAATGTTGA GCTTTAAGCC AGCCTTTTTA CTCTCCTCTT TCACTTTCAT CAAGAGGCTC TTTAGTTCCT CTTCACTTTC TGCCATAAGG GTGGTGTCAT CTGCATATCT GAGGTTATTG ATATTTCTCT TGGCAATTTT GATTCCAGCC TGCACTTCTT CCAGCCCAGT GTTTCTCATG ATGTACTCTG CATATAAATT AAATAAGCAG AGTGACAATA TACAGCCTTG ACATACTCTT TTTCCTATTT GGAACCAGTC TGTTGTTCCA TGTCCAGTTC TAACTGTTGT TTCCTGACCT GCATACAGGT TTCTCAAGAG GCAAGTCAGG TGGTCTGGTA TTCTCACCTG TTTCAGAATT TTCCACAGTT TATTGTGATC CACACAGTCA AAGGCTTTGG CATAGCCAAT AAAGCAGAAA GAGATGTTTT TCTGGAACTC TCTTACTTTT TTGATGATCC AGTGGATGTT GGCAATTTGA TCTCTGGTTC CTCTGCCTTT TCTAAAACCA GCTTTAACAT CTGGAAGTTC ATGGTTCACG TAATACAAAA TGTAATACAA AATGTCTGCA AAAACAAAGG AATGAAAAGT AATGCTAAAA AATGTTAATA TTTACAGAAA TTTTTATAGT AGTAAAGAAT TCACCTGCAA TACAGGAGAA CCGGGTTAGA TCCCTGGGTT GGAAGACCTC CTGGAGAAGG AAATGGCTAC CCAATCTAGT ATTCTTGTCT GGAGAAGGCA AGAATGGACA GAGAAGCCCA GCGGGCTATG GTCCATCGGG TCACAAAGAG TCAGAAGCTA CCTTGCACAC AGCAAGCACG GTGCGCGCGC GTGCACACAC ACACACACAC ACACACACAG ACACACACAC ACTCTAAAAC ATTTACCCAA GCTTGTCCAA TGGAAAATCA AAAAGCCAGC AATTTAAGAT GACATCAGGT ACCACTGTCC AGGTAAGCCT CAGAACACAA TGACCAGTAA GAAGCAAAGT GCCATATGAG CAACTCGAAT TTTTGCAATG TTACCTAAGA GCTTCCATTT TTATAATGCA AAAGAATTTC ATATGGGGAA ATTGTATTAG ATAACCCTGA ATGAGGAGCA AGATATAGTC AAAGTAAGAT GCTCTAGTAC TATTTTTTAT AAGCATGATT TGTTCAGCCA AAGGTTTTTT CCCATATGGC CAATGAACTG AAATATGCAG TCCTGAGATT TGCATATATT TCTAGCTGAA ACCAAGTAAA TAATATCCTC AAGAAAGAAA TCAATAGAAA AGTTGGATGA AGAGTACAAT AAAGGGACCA AAAATATTCA GAAATAAGAA CTAGAGGAGA TATTGGGAAA TCCCTGGTGA GTCCAGTTTA GGATTTTGTA CTTTCACTGC AGTTGGCATG GATATAATCC CTCACTGGGG AACTAAGATC CCATAAGCTG TGTTGGATTG CCAAAAAAAT AAATATTAAG AGATATCATT CATAGAATAT TTTAAAGATA TTTTAGAGAA GAGGAAATTA AGGATGTGAG AATTTGTATT ACTTTTTCAA GATACTAAAG CTATTTAGAG ATAGAGCTGT TACTAAAAAC TTCAGTTTCC TAAAAATTAT TTGAAGCACT GTTTAATAAA TTCCAAAATA TAGAGGAAGG AAAACAAAAT ACTGAGGATT CATATAATGA TTCAGATTTA GAAACAATAT AACACAGAAT TAGTGAATTC TGACAAATTA TTAGGTAGGA GTAGATAGTT CAGCATTACT CGTATAGATG GAGTATTTAA TCCTTTCCAT GAGATTATCC AAATATAATA ATTTCGTATC TATGTGAAGT ATAACTATTA AGATTACTTT ATAAAGTAAA TCAAGAACCA GAGAATAAGA AAAATGTTTT GTGAACCAGC AGATACTATG AACACATAAA ACTCAGAACC CTGATTCCTA AGACACACAG CTAATCCTGA TTATTCTTCC TTTACATGTG ACCATAGAAC TTCACACAAG TTCAAGATAC ATTTGTTGAG CACATCAGTA TCAGTTCAGT CACTCAGTCA TGTCCGAATC TTTGTGACCT TGTGGACTGC AGCACGCCAG GCTTTCCTGT CCACCACCAA CCCCTGGAGC TTACTCAAAC TCATGTCCAT TGAGTCAGTG ATCCCATCCA ACCATCTCAT CCTCTGTCAT CCTCTTCTCC TGCCTTCAAT CTTTCCCAGA CATTGGAGTC TTTTCCAATG AGTCAGATCT TCACATTAGG TGGCCAAAGT ATAGGAGTTT CAGCTTCAGC ATCAATCCTT CCAATGAATA TTCCTTGATG TACCCCTTTC GCAGTTTGGA ACCAGTCTGT TGTTCCATGT CCAGTTCTAA CTGCTGCTTC TGGACCTGTA TACAGATTTC TCAGGAGGCA GGTAAAGTGG TCTGGTATTC CCATCTCTTG AAGAATTTTC CACAGTTTAT TGTGATCCAC ACAATCAAAG GCTTTAGCGT AGTCAATAAA GCAGATGTTT TTCTGGAACT CTCGTGCTTT TTTGATGATC CAATGGATGT TGGCAATTTG ATCTCTGGTT CCTCTGCCTT TTCTAAATCC AGCTTGAACA TCTGGAAGTT CATGGTCCAC GTACTGTTGA AGCCTGGCTT GGAGAATTTT GAGAGTTATT TTGCTAGCAT GTGAGATGAG TGCAATCATG TGGGTGTTTG AACATACTTT GTCATTGCTT TTCTTTGGGA TTGTGGCAGT CCTGTGGCCA CTGCTGAGTT TTCCAAATTT GCTGACATAT TGAGTGCAGC ACTTTCACAG CATCACCTTT TAAGATTTGA AATAGCTCAA CTGGAATTCC ATCACCTCCA CTAGCTTTGT TCATAGTGAG GCTTTCTAAG GCCGTTTGAC TTTGCATTCC AGGGTGTCTG GCTCTAGGTG AGTGATCCGT TGACCTGCAG CGGCCGCAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGAGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTTGAT GCCTCCGTGT AAGGGGGAAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCTATTAA TACGATACCT GCGTCATAAT TGATTATTTG ACGTGGTTTG ATGGCCTCCA CGCACGTTGT GATATGTAGA TGATAATCAT TATCACTTTA CGGGTCCTTT CCGGTGATCC GACAGGTTAC GGGGCGGCGA CCTCGCGGGT TTTCGCTATT TATGAAAATT TTCCGGTTTA AGGCGTTTCC GTTCTTCTTC GTCATAACTT AATGTTTTTA TTTAAAATAC CCTCTGAAAA GAAAGGAAAC GACAGGTGCT GAAAGCGAGG CTTTTTGGCC TCTGTCGTTT CCTTTCTCTG TTTTTGTCCG TGGAATGAAC AATGGAAGTC AACAAAAAGC AGCTGGCTGA CATTTTCGGT GCGAGTATCC GTACCATTCA GAACTGGCAG GAACAGGGAA TGCCCGTTCT GCGAGGCGGT GGCAAGGGTA ATGAGGTGCT TTATGACTCT GCCGCCGTCA TAAAATGGTA TGCCGAAAGG GATGCTGAAA TTGAGAACGA
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