pOTB7-LILRB1
$113.00
In stock
SKU
PL83714
LT742769.1;ILT2;LIR1;MIR7;CD85J;ILT-2;LIR-1;MIR-7
100μg liquid plasmid(Instantly):$134
| name | pOTB7-LILRB1 | another name | apOTB7-LILRB1 |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Gene template | Fragment type | cDNA |
| Fragment species | Human | Prokaryotic resistance | Chl |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pOTB7 | 5′ sequencing primer | M13F：TGTAAAACGACGGCCAGT |
| 3′ sequencing primer | M13R：CAGGAAACAGCTATGACC | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CGTTAGAACG CGGCTACAAT TAATACATAA CCTTATGTAT CATACACATA CGATTTAGGT GACACTATAG AACTCGAAGG ATCCACAAGT TTGTACAAAA AAGCAGGCTT GTAAAACGAC GGCCAGTAAC TATAACGGTC CTAAGGTAGC GAGGCCTGGG TGGCGAATTC GGCACGAGGG AGGAGGAACA GAAAAGAAAA GAAAAGAAAA AGTGGGAAAC AAATAATCTA AGAATGAGGA GAAAGCAAGA AGAGTGACCC CCTTGTGGGC ACTCCATTGG TTTTATGGCG CCTCTACTTT CTGGAGTTTG TGTAAAACAA AAATATTATG GTCTTTGTGC ACATTTACAT CAAGCTCAGC CTGGGCGGCA CAGCCAGATG CGAGATGCGT CTCTGCTGAT CTGAGTCTGC CTGCAGCATG GACCTGGGTC TTCCCTGAAG CATCTCCAGG GCTGGAGGGA CGACTGCCAT GCACCGAGGG CTCATCCATC CACAGAGCAG GGCAGTGGGA GGAGACGCCA TGACCCCCAT CCTCACGGTC CTGATCTGTC TCGGGCTGAG TCTGGGCCCC CGGACCCACG TGCAGGCAGG GCACCTCCCC AAGCCCACCC TCTGGGCTGA ACCAGGCTCT GTGATCACCC AGGGGAGTCC TGTGACCCTC AGGTGTCAGG GGGGCCAGGA GACCCAGGAG TACCGTCTAT ATAGAGAAAA GAAAACAGCA CCCTGGATTA CACGGATCCC ACAGGAGCTT GTGAAGAAGG GCCAGTTCCC CATCCCATCC ATCACCTGGG AACATGCAGG GCGGTATCGC TGTTACTATG GTAGCGACAC TGCAGGCCGC TCAGAGAGCA GTGACCCCCT GGAGCTGGTG GTGACAGGAG CCTACATCAA ACCCACCCTC TCAGCCCAGC CCAGCCCCGT GGTGAACTCA GGAGGGAATG TAACCCTCCA GTGTGACTCA CAGGTGGCAT TTGATGGCTT CATTCTGTGT AAGGAAGGAG AAGATGAACA CCCACAATGC CTGAACTCCC AGCCCCATGC CCGTGGGTCG TCCCGCGCCA TCTTCTCCGT GGGCCCCGTG AGCCCGAGTC GCAGGTGGTG GTACAGGTGC TATGCTTATG ACTCGAACTC TCCCTATGAG TGGTCTCTAC CCAGTGATCT CCTGGAGCTC CTGGTCCTAG GTGTTTCTAA GAAGCCATCA CTCTCAGTGC AGCCAGGTCC TATCGTGGCC CCTGAGGAGA CCCTGACTCT GCAGTGTGGC TCTGATGCTG GCTACAACAG ATTTGTTCTG TATAAGGACG GGGAACGTGA CTTCCTTCAG CTCGCTGGCG CACAGCCCCA GGCTGGGCTC TCCCAGGCCA ACTTCACCCT GGGCCCTGTG AGCCGCTCCT ACGGGGGCCA GTACAGATGC TACGGTGCAC ACAACCTCTC CTCCGAGTGG TCGGCCCCCA GCGACCCCCT GGACATCCTG ATCGCAGGAC AGTTCTATGA CAGAGTCTCC CTCTCGGTGC AGCCGGGCCC CACGGTGGCC TCAGGAGAGA ACGTGACCCT GCTGTGTCAG TCACAGGGAT GGATGCAAAC TTTCCTTCTG ACCAAGGAGG GGGCAGCTGA TGACCCATGG CGTCTAAGAT CAACGTACCA ATCTCAAAAA TACCAGGCTG AATTCCCCAT GGGTCCTGTG ACCTCAGCCC ATGCGGGGAC CTACAGGTGC TACGGCTCAC AGAGCTCCAA ACCCTACCTG CTGACTCACC CCAGTGACCC CCTGGAGCTC GTGGTCTCAG GACCGTCTGG GGGCCCCAGC TCCCCGACAA CAGGCCCCAC CTCCACATCT GGCCCTGAGG ACCAGCCCCT CACCCCCACC GGGTCGGATC CCCAGAGTGG TCTGGGAAGG CACCTGGGGG TTGTGATCGG CATCTTGGTG GCCGTCATCC TACTGCTCCT CCTCCTCCTC CTCCTCTTCC TCATCCTCCG ACATCGACGT CAGGGCAAAC ACTGGACATC GACCCAGAGA AAGGCTGATT TCCAACATCC TGCAGGGGCT GTGGGGCCAG AGCCCACAGA CAGAGGCCTG CAGTGGAGGT CCAGCCCAGC TGCCGATGCC CAGGAAGAAA ACCTCTATGC TGCCGTGAAG CACACACAGC CTGAGGATGG GGTGGAGATG GACACTCGGA GCCCACACGA TGAAGACCCC CAGGCAGTGA CGTATGCCGA GGTGAAACAC TCCAGACCTA GGAGAGAAAT GGCCTCTCCT CCTTCCCCAC TGTCTGGGGA ATTCCTGGAC ACAAAGGACA GACAGGCGGA AGAGGACAGG CAGATGGACA CTGAGGCTGC TGCATCTGAA GCCCCCCAGG ATGTGACCTA CGCCCAGCTG CACAGCTTGA CCCTTAGACG GAAGGCAACT GAGCCTCCTC CATCCCAGGA AGGGCCCTCT CCAGCTGTGC CCAGCATCTA CGCCACTCTG GCCATCCACT AGCCCAGGGG GGGACGCAGA CCCCACACTC CATGGAGTCT GGAATGCATG GGAGCTGCCC CCCCAGTGGA CACCATTGGA CCCCACCCAG CCTGGATCTA CCCCAGGAGA CTCTGGGAAC TTTTAGGGGT CACTCAATTC TGCAGTATAA ATAACTAATG TCTCTACAAT TTTGAAATAA AGCAACAGAC TTTTCAATAA TCAATGAAGT AGCTGAGAAA ACTAAGTCAG AAAGTGCATT AAACTGAATC CCAATGTAAA TATTACACAT CAAGCGATGA AACTGGAAAA CTACAAGCCC CGAATGAATG AATTAGGAAA GAAAAAAAGT AGGAAATGAA TGATTTTGGC TTTCCTATAA GAAATTTAGG GCAGGGCACG GTGGCTCACG CCTGTAATTC CAGCACTTTG GGAGGCCGAG GCGGGCAGAT CACGAGTTCA GGAGATCGAG ACCATCTTGG CCAACATGGT GAAACCCTGT CTCTCCTAAA AATACAAAAA TTAGCTGGAT GTGGTGGCAG TGCCTGTAAT CCCAGCTATT TGGGAGGCTG AGGCAGGAGA ATCGCTTGAA CCAGGGAGTC AGAGGTTTCA GTGAGCCAAG ATCGCACCCC TGCTCTCCAG CCTGGCGACA GAGGGAGACT CCATCTCAAA TTAAAAAAAA AAAAAAAAAA GAAAGAAAAA AAAAAAAAAA AAACTCGAGG CATCTATGTC GGGTGCGGAG AAAGAGGTAA TGAAATGGCA CATGGTCATA GCTGTTTCCT GACCCAGCTT TCTTGTACAA AGTGGTAGAT CTGCCGGTCT CCCTATAGTG AGTCGTATTA ATTTCGATAA GCCAGGTTAA CCTGCATTAA TGAATCGGCT GCAGTACCCG GGAATTTAAC CCGCCTAATG AGCGGGCTTT TTTTTGTGAT CCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGAT CACAACAAAA AGCCCGCTCA TTAGGCGGGC TAAATTCTCA TGTTTGACAG CTTATCATCG ATAAGCTAGC GGCCGCTAGC TTTAATGAGT TATCGAGATT TTCAGGAGCT AAGGAAGCTA AAATGGAGAA AAAAATCACT GGATATACCA CCGTTGATAT ATCCCAATGG CATCGTAAAG AACATTTTGA GGCATTTCAG TCAGTTGCTC AATGTACCTA TAACCAGACC GTTCAGCTGG ATATTACGGC CTTTTTAAAG ACCGTAAAGA AAAATAAGCA CAAGTTTTAT CCGGCCTTTA TTCACATTCT TGCCCGCCTG ATGAATGCTC ATCCGGAGTT CCGTATGGCA ATGAAAGACG GTGAGCTGGT GATATGGGAT AGTGTTCACC CTTGTTACAC CGTTTTCCAT GAGCAAACTG AAACGTTTTC ATCGCTCTGG AGTGAATACC ACGACGATTT CCGGCAGTTT CTACACATAT ATTCGCAAGA TGTGGCGTGT TACGGTGAAA ACCTGGCCTA TTTCCCTAAA GGGTTTATTG AGAATATGTT TTTCGTCTCA GCCAATCCCT GGGTGAGTTT CACCAGTTTT GATTTAAACG TGGCCAATAT GGACAACTTC TTCGCCCCCG TTTTCACCAT GGGCAAATAT TATACGCAAG GCGACAAGGT GCTGATGCCG CTGGCGATTC AGGTTCATCA TGCCGTTTGT GATGGCTTCC ATGTCGGCAG AATGCTTAAT GAATTACAAC AGTACTGCGA TGAGTGGCAG GGCGGGGCGT AATTGGTACG TCGA
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