pRSETB-ECFP-GABABR-m-Ypet
$113.00
In stock
SKU
PL83666
100μg liquid plasmid(Instantly):$134
| name | pRSETB-ECFP-GABABR-m-Ypet | another name | apRSETB-ECFP-GABABR-m-Ypet |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | T7 | Replicon | pBR322 |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | T7：TAATACGACTCACTATAGGG | |
| 3′ sequencing primer | T7ter：TGCTAGTTATTGCTCAGCGG | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GATCTCGATC CCGCGAAATT AATACGACTC ACTATAGGGA GACCACAACG GTTTCCCTCT AGAAATAATT TTGTTTAACT TTAAGAAGGA GATATACATA TGCGGGGTTC TCATCATCAT CATCATCATG GTATGGCTAG CATGACTGGT GGACAGCAAA TGGGTCGGGA TCTGTACGAC GATGACGATA AGGATCCGAT GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC CTGACCTGGG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC AACTACATCA GCCACAACGT CTATATCACC GCCGACAAGC AGAAGAACGG CATCAAGGCC AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCACC CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGCTCGA GATGGGCCCG GGGGGACCCT GTACCCCGGT GGGGTGGCCG CTGCCTCTTC TGCTGGTGAT GGCGGCTGGG GTGGCTCCGG TGTGGGCCTC TCACTCCCCT CATCTCCCGC GGCCTCACCC GAGGGTCCCC CCGCACCCCT CCTCAGAACG GCGTGCAGTA TACATCGGGG CGCTGTTTCC CATGAGCGGG GGCTGGCCGG GGGGCCAGGC CTGCCAGCCT GCGGTGGAGA TGGCGCTGGA GGACGTTAAC AGCCGCAGAG ACATCCTGCC GGACTACGAG CTCAAGCTTA TCCACCACGA CAGCAAGTGC GACCCAGGGC AAGCCACCAA GTACTTGTAT GAACTACTCT ACAACGACCC CATCAAGATC ATCCTCATGC CCGGCTGCAG CTCTGTGTCC ACACTGGTAG CCGAGGCTGC CCGGATGTGG AACCTTATTG TGCTCTCATA TGGCTCCAGC TCACCAGCCT TGTCAAACCG ACAGCGGTTT CCAACGTTCT TTCGGACACA TCCATCCGCC ACACTCCACA ATCCCACCCG GGTGAAACTC TTCGAAAAGT GGGGCTGGAA GAAGATTGCC ACCATCCAGC AGACTACCGA GGTCTTCACC TCAACACTGG ATGACCTGGA GGAGCGAGTG AAAGAGGCTG GGATTGAGAT CACTTTTCGA CAGAGTTTCT TCTCAGATCC AGCTGTGCCT GTTAAAAACC TGAAGCGTCA AGATGCTCGA ATCATCGTGG GACTTTTCTA TGAGACCGAA GCCCGGAAAG TTTTTTGTGA GGTCTATAAG GAACGGCTCT TTGGGAAGAA GTATGTCTGG TTTCTCATCG GGTGGTATGC TGACAACTGG TTCAAAACCT ATGACCCGTC AATCAATTGT ACAGTAGAAG AGATGACTGA GGCGGTGGAG GGCCATATCA CCACGGAGAT TGTCATGCTG AACCCTGCCA ACACCCGAAG CATTTCCAAC ATGACATCAC AGGAATTTGT GGAGAAACTA ACCAAGCGGC TGAAAAGACA CCCTGAGGAG ACTGGAGGCT TCCAGGAGGC ACCACTGGCC TATGATGCTA TCTGGGCCTT GGCTTTGGCC TTGAACAAGA CCTCTGGAGG AGGTGGCCGT TCAGGAGTGC GCCTGGAGGA CTTTAACTAC AACAACCAGA CCATTACAGA CCAAATCTAC CGGGCCATGA ACTCCTCCTC CTTTGAGGGT GTTTCTGGCC ACGTGGTCTT TGATGCCAGC GGCTCCCGGA TGGCATGGAC GCTTATCGAG CAGCTACAGG GCGGCAGCTA CAAGAAGATC GGCTACTACG ACAGCACCAA GGATGATCTT TCCTGGTCCA AAACAGACAA GTGGATCGGA GGGTCTCCCC CAGCCGACCA GACCTTGGTC ATCAAGACAT TCCGTTTCCT GTCACAGAAA CTCTTTATCT CCGTCTCAGT TCTCTCCAGC CTGGGCGGTA CCATGTCTAA AGGTGAAGAA TTATTCACTG GTGTTGTCCC AATTTTGGTT GAATTAGATG GTGATGTTAA TGGTCACAAA TTTTCTGTCT CCGGTGAAGG TGAAGGTGAT GCTACGTACG GTAAATTGAC CTTAAAATTA CTCTGTACTA CTGGTAAATT GCCAGTTCCA TGGCCAACCT TAGTCACTAC TTTAGGTTAT GGTGTTCAAT GTTTTGCTAG ATACCCAGAT CATATGAAAC AACATGACTT TTTCAAGTCT GCCATGCCAG AAGGTTATGT TCAAGAAAGA ACTATTTTTT TCAAAGATGA CGGTAACTAC AAGACCAGAG CTGAAGTCAA GTTTGAAGGT GATACCTTAG TTAATAGAAT CGAATTAAAA GGTATTGATT TTAAAGAAGA TGGTAACATT TTAGGTCACA AATTGGAATA CAACTATAAC TCTCACAATG TTTACATCAC TGCTGACAAA CAAAAGAATG GTATCAAAGC TAACTTCAAA ATTAGACACA ACATTGAAGA TGGTGGTGTT CAATTAGCTG ACCATTATCA ACAAAATACT CCAATTGGTG ATGGTCCAGT CTTGTTACCA GACAACCATT ACTTATCCTA TCAATCTGCC TTATTCAAAG ATCCAAACGA AAAGAGAGAC CACATGGTCT TGTTAGAATT TTTGACTGCT GCTGGTATTA CCGAGGGTAT GAATGAATTG TACAAATGAG AATTCGAAGC TTGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATCT GGCGTAATAG CGAAGAGGCC CGCACCGATC GCCCTTCCCA ACAGTTGCGC AGCCTGAATG GCGAATGGGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT AACAAAATAT TAACGCTTAC AATTTAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA G
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