pHT43
$55.00
In stock
SKU
PL83644
DB00061;pHT-43
100μg liquid plasmid(Instantly):$57
| name | pHT43 | another name | apHT43 |
| Insert Size (bp) | Plasmid host | Bacillus subtilis | |
| Plasmid uses | Protein expression,Secretion expression | Fragment type | ORF |
| Fragment species | Empty vector | Prokaryotic resistance | Amp |
| Selective marker | Chl | Fluorescent labeling | |
| Promoter | Pgrac | Replicon | pUC,repB |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | M13F：TGTAAAACGACGGCCAGT | |
| 3′ sequencing primer | pHT-R：agcaagtcatctccaaattctt | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TTAAGTTATT GGTATGACTG GTTTTAAGCG CAAAAAAAGT TGCTTTTTCG TACCTATTAA TGTATCGTTT TAGAAAACCG ACTGTAAAAA GTACAGTCGG CATTATCTCA TATTATAAAA GCCAGTCATT AGGCCTATCT GACAATTCCT GAATAGAGTT CATAAACAAT CCTGCATGAT AACCATCACA AACAGAATGA TGTACCTGTA AAGATAGCGG TAAATATATT GAATTACCTT TATTAATGAA TTTTCCTGCT GTAATAATGG GTAGAAGGTA ATTACTATTA TTATTGATAT TTAAGTTAAA CCCAGTAAAT GAAGTCCATG GAATAATAGA AAGAGAAAAA GCATTTTCAG GTATAGGTGT TTTGGGAAAC AATTTCCCCG AACCATTATA TTTCTCTACA TCAGAAAGGT ATAAATCATA AAACTCTTTG AAGTCATTCT TTACAGGAGT CCAAATACCA GAGAATGTTT TAGATACACC ATCAAAAATT GTATAAAGTG GCTCTAACTT ATCCCAATAA CCTAACTCTC CGTCGCTATT GTAACCAGTT CTAAAAGCTG TATTTGAGTT TATCACCCTT GTCACTAAGA AAATAAATGC AGGGTAAAAT TTATATCCTT CTTGTTTTAT GTTTCGGTAT AAAACACTAA TATCAATTTC TGTGGTTATA CTAAAAGTCG TTTGTTGGTT CAAATAATGA TTAAATATCT CTTTTCTCTT CCAATTGTCT AAATCAATTT TATTAAAGTT CATTTGATAT GCCTCCTAAA TTTTTATCTA AAGTGAATTT AGGAGGCTTA CTTGTCTGCT TTCTTCATTA GAATCAATCC TTTTTTAAAA GTCAATATTA CTGTAACATA AATATATATT TTAAAAATAT CCCACTTTAT CCAATTTTCG TTTGTTGAAC TAATGGGTGC TTTAGTTGAA GAATAAAGAC CACATTAAAA AATGTGGTCT TTTGTGTTTT TTTAAAGGAT TTGAGCGTAG CGAAAAATCC TTTTCTTTCT TATCTTGATA ATAAGGGTAA CTATTGCCGT CGTCCATTCC GACAGCATCG CCAGTCACTA TGGCGTGCTG CTAGCGCCAT TCGCCATTCA GGCTGCGCAA CTGTTGGGAA GGGCGATCGG TGCGGGCCTC TTCGCTATTA CGCCAGCTGG CGAAAGGGGG ATGTGCTGCA AGGCGATTAA GTTGGGTAAC GCCAGGGTTT TCCCAGTCAC GACGTTGTAA AACGACGGCC AGTGAATTCG AGCTCAGGCC TTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCCA GGGTGGTTTT TCTTTTCACC AGTGAGACGG GCAACAGCTG ATTGCCCTTC ACCGCCTGGC CCTGAGAGAG TTGCAGCAAG CGGTCCACGC TGGTTTGCCC CAGCAGGCGA AAATCCTGTT TGATGGTGGT TGACGGCGGG ATATAACATG AGCTGTCTTC GGTATCGTCG TATCCCACTA CCGAGATATC CGCACCAACG CGCAGCCCGG ACTCGGTAAT GGCGCGCATT GCGCCCAGCG CCATCTGATC GTTGGCAACC AGCATCGCAG TGGGAACGAT GCCCTCATTC AGCATTTGCA TGGTTTGTTG AAAACCGGAC ATGGCACTCC AGTCGCCTTC CCGTTCCGCT ATCGGCTGAA TTTGATTGCG AGTGAGATAT TTATGCCAGC CAGCCAGACG CAGACGCGCC GAGACAGAAC TTAATGGGCC CGCTAACAGC GCGATTTGCT GGTGACCCAA TGCGACCAGA TGCTCCACGC CCAGTCGCGT ACCGTCTTCA TGGGAGAAAA TAATACTGTT GATGGGTGTC TGGTCAGAGA CATCAAGAAA TAACGCCGGA ACATTAGTGC AGGCAGCTTC CACAGCAATG GCATCCTGGT CATCCAGCGG ATAGTTAATG ATCAGCCCAC TGACGCGTTG CGCGAGAAGA TTGTGCACCG CCGCTTTACA GGCTTCGACG CCGCTTCGTT CTACCATCGA CACCACCACG CTGGCACCCA GTTGATCGGC GCGAGATTTA ATCGCCGCGA CAATTTGCGA CGGCGCGTGC AGGGCCAGAC TGGAGGTGGC AACGCCAATC AGCAACGACT GTTTGCCCGC CAGTTGTTGT GCCACGCGGT TGGGAATGTA ATTCAGCTCC GCCATCGCCG CTTCCACTTT TTCCCGCGTT TTCGCAGAAA CGTGGCTGGC CTGGTTCACC ACGCGGGAAA CGGTCTGATA AGAGACACCG GCATACTCTG CGACATCGTA TAACGTTACT GGTTTCATCA AAATCGTCTC CCTCCGTTTG AATATTTGAT TGATCGTAAC CAGATGAAGC ACTCTTTCCA CTATCCCTAC AGTGTTATGG CTTGAACAAT CACGAAACAA TAATTGGTAC GTACGATCTT TCAGCCGACT CAAACATCAA ATCTTACAAA TGTAGTCTTT GAAAGTATTA CATATGTAAG ATTTAAATGC AACCGTTTTT TCGGAAGGAA ATGATGACCT CGTTTCCACC GGAATTAGCT TGGTACCAGC TATTGTAACA TAATCGGTAC GGGGGTGAAA AAGCTAACGG AAAAGGGAGC GGAAAAGAAT GATGTAAGCG TGAAAAATTT TTTATCTTAT CACTTGAAAT TGGAAGGGAG ATTCTTTATT ATAAGAATTG TGGAATTGTG AGCGGATAAC AATTCCCAAT TAAAGGAGGA AGGATCAATG ATTCAAAAAC GAAAGCGGAC AGTTTCGTTC AGACTTGTGC TTATGTGCAC GCTGTTATTT GTCAGTTTGC CGATTACAAA AACATCAGCC GTAGGATCCT CTAGAGTCGA CGTCCCCGGG GCAGCCCGCC TAATGAGCGG GCTTTTTTCA CGTCACGCGT CCATGGAGAT CTTTGTCTGC AACTGAAAAG TTTATACCTT ACCTGGAACA AATGGTTGAA ACATACGAGG CTAATATCGG CTTATTAGGA ATAGTCCCTG TACTAATAAA ATCAGGTGGA TCAGTTGATC AGTATATTTT GGACGAAGCT CGGAAAGAAT TTGGAGATGA CTTGCTTAAT TCCACAATTA AATTAAGGGA AAGAATAAAG CGATTTGATG TTCAAGGAAT CACGGAAGAA GATACTCATG ATAAAGAAGC TCTAAAACTA TTCAATAACC TTACAATGGA ATTGATCGAA AGGGTGGAAG GTTAATGGTA CGAAAATTAG GGGATCTACC TAGAAAGCCA CAAGGCGATA GGTCAAGCTT AAAGAACCCT TACATGGATC TTACAGATTC TGAAAGTAAA GAAACAACAG AGGTTAAACA AACAGAACCA AAAAGAAAAA AAGCATTGTT GAAAACAATG AAAGTTGATG TTTCAATCCA TAATAAGATT AAATCGCTGC ACGAAATTCT GGCAGCATCC GAAGGGAATT CATATTACTT AGAGGATACT ATTGAGAGAG CTATTGATAA GATGGTTGAG ACATTACCTG AGAGCCAAAA AACTTTTTAT GAATATGAAT TAAAAAAAAG AACCAACAAA GGCTGAGACA GACTCCAAAC GAGTCTGTTT TTTTAAAAAA AATATTAGGA GCATTGAATA TATATTAGAG AATTAAGAAA GACATGGGAA TAAAAATATT TTAAATCCAG TAAAAATATG ATAAGATTAT TTCAGAATAT GAAGAACTCT GTTTGTTTTT GATGAAAAAA CAAACAAAAA AAATCCACCT AACGGAATCT CAATTTAACT AACAGCGGCC AAACTGAGAA GTTAAATTTG AGAAGGGGAA AAGGCGGATT TATACTTGTA TTTAACTATC TCCATTTTAA CATTTTATTA AACCCCATAC AAGTGAAAAT CCTCTTTTAC ACTGTTCCTT TAGGTGATCG CGGAGGGACA TTATGAGTGA AGTAAACCTA AAAGGAAATA CAGATGAATT AGTGTATTAT CGACAGCAAA CCACTGGAAA TAAAATCGCC AGGAAGAGAA TCAAAAAAGG GAAAGAAGAA GTTTATTATG TTGCTGAAAC GGAAGAGAAG ATATGGACAG AAGAGCAAAT AAAAAACTTT TCTTTAGACA AATTTGGTAC GCATATACCT TACATAGAAG GTCATTATAC AATCTTAAAT AATTACTTCT TTGATTTTTG GGGCTATTTT TTAGGTGCTG AAGGAATTGC GCTCTATGCT CACCTAACTC GTTATGCATA CGGCAGCAAA GACTTTTGCT TTCCTAGTCT ACAAACAATC GCTAAAAAAA TGGACAAGAC TCCTGTTACA GTTAGAGGCT ACTTGAAACT GCTTGAAAGG TACGGTTTTA TTTGGAAGGT AAACGTCCGT AATAAAACCA AGGATAACAC AGAGGAATCC CCGATTTTTA AGATTAGACG TAAGGTTCCT TTGCTTTCAG AAGAACTTTT AAATGGAAAC CCTAATATTG AAATTCCAGA TGACGAGGAA GCACATGTAA AGAAGGCTTT AAAAAAGGAA AAAGAGGGTC TTCCAAAGGT TTTGAAAAAA GAGCACGATG AATTTGTTAA AAAAATGATG GATGAGTCAG AAACAATTAA TATTCCAGAG GCCTTACAAT ATGACACAAT GTATGAAGAT ATACTCAGTA AAGGAGAAAT TCGAAAAGAA ATCAAAAAAC AAATACCTAA TCCTACAACA TCTTTTGAGA GTATATCAAT GACAACTGAA GAGGAAAAAG TCGACAGTAC TTTAAAAAGC GAAATGCAAA ATCGTGTCTC TAAGCCTTCT TTTGATACCT GGTTTAAAAA CACTAAGATC AAAATTGAAA ATAAAAATTG TTTATTACTT GTACCGAGTG AATTTGCATT TGAATGGATT AAGAAAAGAT ATTTAGAAAC AATTAAAACA GTCCTTGAAG AAGCTGGATA TGTTTTCGAA AAAATCGAAC TAAGAAAAGT GCAATAAACT GCTGAAGTAT TTCAGCAGTT TTTTTTATTT AGAAATAGTG AAAAAAATAT AATCAGGGAG GTATCAATAT TTAATGAGTA CTGATTTAAA TTTATTTAGA CTGGAATTAA TAATTAACAC GTAGACTAAT TAAAATTTAA TGAGGGATAA AGAGGATACA AAAATATTAA TTTCAATCCC TATTAAATTT TAACAAGGGG GGGATTAAAA TTTAATTAGA GGTTTATCCA CAAGAAAAGA CCCTAATAAA ATTTTTACTA GGGTTATAAC ACTGATTAAT TTCTTAATGG GGGAGGGATT AAAATTTAAT GACAAAGAAA ACAATCTTTT AAGAAAAGCT TTTAAAAGAT AATAATAAAA AGAGCTTTGC GATTAAGCAA AACTCTTTAC TTTTTCATTG ACATTATCAA ATTCATCGAT TTCAAATTGT TGTTGTATCA TAAAGTTAAT TCTGTTTTGC ACAACCTTTT CAGGAATATA AAACACATCT GAGGCTTGTT TTATAAACTC AGGGTCGCTA AAGTCAATGT AACGTAGCAT ATGATATGGT ATAGCTTCCA CCCAAGTTAG CCTTTCTGCT TCTTCTGAAT GTTTTTCATA TACTTCCATG GGTATCTCTA AATGATTTTC CTCATGTAGC AAGGTATGAG CAAAAAGTTT ATGGAATTGA TAGTTCCTCT CTTTTTCTTC AACTTTTTTA TCTAAAACAA ACACTTTAAC ATCTGAGTCA ATGTAAGCAT AAGATGTTTT TCCAGTCATA ATTTCAATCC CAAATCTTTT AGACAGAAAT TCTGGACGTA AATCTTTTGG TGAAAGAATT TTTTTATGTA GCAATATATC CGATACAGCA CCTTCTAAAA GCGTTGGTGA ATAGGGCATT TTACCTATCT CCTCTCATTT TGTGGAATAA AAATAGTCAT ATTCGTCCAT CTACCTATCC TATTATCGAA CAGTTGAACT TTTTAATCAA GGATCAGTCC TTTTTTTCAT TATTCTTAAA CTGTGCTCTT AACTTTAACA ACTCGATTTG TTTTTCCAGA TCTCGAGGGT AACTAGCCTC GCCGATCCCG CAAGAGGCCC GGCAGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCATGC
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