pHT304
$55.00
In stock
SKU
PL83637
DB00107;pHT-304
100μg liquid plasmid(Instantly):$57
| name | pHT304 | another name | apHT304 |
| Insert Size (bp) | Plasmid host | Bacillus subtilis | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Empty vector | Prokaryotic resistance | Amp |
| Selective marker | Ery | Fluorescent labeling | |
| Promoter | Pgrac | Replicon | pUC,repB |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | M13R：CAGGAAACAGCTATGACC | |
| 3′ sequencing primer | M13F：TGTAAAACGACGGCCAGT | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CCATCCTCCA AAGTTGGAGA GTGAGTTTTA TGTCGCAAAT ATTAATGTTT CTGGTGAACC TTATCAAATT TTCGTTGATT TAATAGAAAC ATAGCGGTAA AATTAGCAGT AACTTAATAG AACGGAAATG AAAAAAGCCA CTCTCATATG CTATTGGCTA CCAACCTTTA GCGAGAATGA CTTAATCCTG TACAGCCATA CAGGACTTCG ACTTATAAGA GGCGCCAACC TCAAATAAGT TATTTGCCTT GTTTTCGCGA ACAAGGCTTA TTAGATACAC CTATTGTACC GTTACTCTAC GAATATTTCA ACTAGTAATT ACTAGCATTG TCATATACAT AATAAAACGG ATATAAAAGG GCGTTTTCTA TACCTAGAAG TCTTGTAAAT GTACAGGGCG TTTAGATATA GAGAACGCCC TTTTTGTGTT CCGTTCCAGT GGAGGCTACC ACTTTAAAAA GATGGTCTAG TGTAGCCAAT GCAGGAGAGT ACACTCGGAT ATCAGTTGTC GTTGCATTCA ACTGTCTGAC GTAAGCGAGG TAAAGGACAC AAGCCTTGCA TAAAACAAGC CTACGGGATG TAAATCCTAA TAATGATGAT AACCAAGACG TTAGCGGCAA AAAGTGTTGG GGGTTCAAAA TAAGACATGA TTGTGCGACT GGAGTTAAAC AGTTACTCGT AAGCGGCGAT CATGACACTG ATTCACGGCT ATTCTTGTAC AAACTTTATT ACAAGGATAT GCGGGTTATA TAGCGAATCA CCCGAAAGGG AACGGTGTTG GGCGTGAGAA ACGCACCGTA CGGCGCAATA CAATGCCAAT AAGCTATATA CGGACGGTAT AGTAGTTTTG TAAGCTATAA CCGTTTGTCG TCAATGCAAC CAATCTCAAT TCGAGACCTC GGCATCTAAG CCAGTACGAA TGAGTGGGCG TTTTAACCTC GTAAATTTTC AACAGGGGTT ACTATGCCCA AAACTACATT CAGATTTCCT AACAAACTCG CCAGTATGAA AACCTTAAGA CCTTAAAGTC AAGGGATTTG AAGGATTTTA ACCTCGATTA GCAAAAAATG TAGAGTACTG AAGCAACTAC CATTAACTAA GATAGTGGGG GATTGAGGAA GAATCCAGAG CTGTTTAAAT CAAGTGAAAG ACAAGATGAA ATTAAAAGAA TAGTGAAAGA TAGGGGAGTG GTTCTCTATG AGAAAGGAAA TGGCTAGAGA ACAAAGGCAG CGGTTTATTG ATCTATTGTT AGACTTTATG GTAAAGAATC CTCATTTATT TGTTAATGGT ACAGAGGATG AAAGTAATAA TGTTGTTACA AAATGTAATA GTGATATTAA AGAGGTTGCG GAGTCATATT TAACTCTTTT ATAGTGAGAG GGTTAAAACT AATTAATATG TATTAAGGCC CAATGTTGGA ATTATTGTAT TTCACTAGGC AACCTACTTA CTAAAAGTAA GATTATCCAT TAGTGGATGT TATAATATTG GGTTTTTTAA CACAATAATC ATCGCCTTTC GGTGTCGTTT GATAGAAAAG TAACCATTAG CGATGAAAAA GTCAATATAA AAAGCCATCC GTAAAAAACG GATGGCTTAC CGTACATAGG ATCGTTGGTA GGGCGGCGTA TCCTACATCT CTGGTAACTG ACCTAGCCAA TCAAATGCTT GAGAACGGCG GTTAGATAAG CGCGTGGGGA ACCTTTCCCA CCTCAAAGAT CCTATATCAT TATTATGTTA CTTTCTACAG GTAGTATACC ATGTTCTTAT ATTTTAGTAA ACTCCCCGTT AGCTTAACAG GTCTTTGTAA GCAATTAAAC GTCCACTATT CAATCGTCTT TGGATTTTCG CAGGACCGTT TTTTAGATCG AACATAGTTG ATAAGAACAA ATAACCGCTT GGGTCCAACT TTATAGCAAT TAGTATATGG TCATTTAAAA TCTTTACCAA TTCAACGCTA TTAGGTTCTT TAGGATTTTG CCCGACATAG TCGGGGTGTT CAACGATATC TTTTATGTGC GATGAATATT TTTCATAAAT ACCAGGATGT TGTTTCTTTA CGTGCTTTAT AAATCCGGGA AACATTTTTA CATCGTTAGA AGTGCAAGTC AAGTTATATG TATCTATAAT GATTTGTGGA AGTTTTGCCA CAACAGTTGG TTTATTTACA ATCTTTTTTT TATTAGCCGT CAAATTTCTC CCTCATCTCG TCTCTTTATA TCTTTATTTT ATCATAAAGG AGTATTTGAA CCGTCGCGCG GGACAGGTTT ATGATAGGGA TATTTTATTG AATAATTGAT GGTATAAGGG ACTTTCATGC TTGGAAAGTG GGGATTATGA ATTAGATGCT TGTCCACAAT ATGTTCCAAT GTAATTAAAA TTTATGTTCC CACCTTGACC AAACATCACG TCCATACTTA AATCGTCCCT CCTTTAATAG GTAAAATATT AATTTACCTT AATAAAAAAA TAATGGATAA TAGTATTCGT CTGAATTTAT ATAATCAGGG GGAACTATTG ATGCTGGGGA TACTATTTAC AGCGGCGCCA TCTACTGATG TCGTAAAGGA TTTGCAAGAT AAAGTTATAT CATTGCAGGA TCATGAGGTA GCGTTTTTGA ACACCACGAT ATCTAATATG TTGATCCCCT TAGAAGCAAA CTTAAGAGTG TGTTGATAGT GCAGTATCTT AAAATTTTGT GTATAATAGG AATTGAAGTT AAATTAGATG CTAAAAATTT GTAATTAAGA AGGAGGGATT CGTCATGTTG GTATTCCAAA TGCGTAATGT AGATAAAACA TCTACTGTTT TGAAACAGAC TAAAAACAGT GATTACGCAG ATAAATAAAT ACGTTAGATT AATTCCTACC AGTGACTAAT CTTATGACTT TTTAAACAGA TAACTAAAAT TACAAACAAA TCGTTTAACT TCTGTATTTA TTTACAGATG TAATCACTTC AGGAGTAATT ACATGAACAA AAATATAAAA TATTCTCAAA ACTTTTTAAC GAGTGAAAAA GTACTCAACC AAATAATAAA ACAATTGAAT TTAAAAGAAA CCGATACCGT TTACGAAATT GGAACAGGTA AAGGGCATTT AACGACGAAA CTGGCTAAAA TAAGTAAACA GGTAACGTCT ATTGAATTAG ACAGTCATCT ATTCAACTTA TCGACAGAAA AATTAAAACT GAACATTCGT GTCACTTTAA TTCACCAAGA TATTCTACAG TTTCAATTCC CTAACAAACA GAGGTATAAA ATTGTTGGGA GTATTCCTTA CCATTTAAGC ACACAAATTA TTAAAAAAGT GGTTTTTGAA AGCCATGCGT CTGACATCTA TCTGATTGTT GAAGAAGGAT TCTACAAGCG TACCTTGGAT ATTCACCGAA CACTAGGGTT GCTCTTGCAC ACTCAAGTCT CGATTCAGCA ATTGCTTAAG CTGCCAGCGG AATGCTTTCA TCCTAAACCA AAAGTAAACA GTGTCTTAAT AAAACTTACC CGCCATACCA CAGATGTTCC AGATAAATAT TGGAAGCTAT ATACGTACTT TGTTTCAAAA TGGGTCAATC GAGAATATCG TCAACTGTTT ACTAAAAATC AGTTTCATCA AGCAATGAAA CACGCCAAAG TAAACAATTT AAGTACCATT ACTTATGAGC AAGTATTGTC TATTTTTAAT AGTTATCTAT TATTTAACGG GAGGAAATAA TTCTATGAGT CGCTTTTTTA AATTTGGAAA GTTACACGTT ACTAAAGGGA ATGGAGATAA ATTATTAGAT ATACTACTGA CAGCTTCCAA GAAGGTAAAG AGGTCCCTAG CGCCTACGGG GAATTTGTAT CGGGATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA ATACGCAAAC CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAGTTA GCTCACTCAT TAGGCACCCC AGGCTTTACA CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGTGAGC GGATAACAAT TTCACACAGG AAACAGCTAT GACCATGATT ACGCCAAGCT TGCATGCCTG CAGGTCGACT CTAGAGGATC CCCGGGTACC GAGCTCGAAT TCACTGGCCG TCGTTTTACA ACGTCGTGAC TGGGAAAACC CTGGCGTTAC CCAACTTAAT CGCCTTGCAG CACATCCCCC TTTCGCCAGC TGGCGTAATA GCGAAGAGGC CCGCACCGAT CGCCCTTCCC AACAGTTGCG CAGCCTGAAT GGCGAATGGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT
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